Effects of Acid-fermented Food Wastewater in Microwave-based Direct Lipid Extraction from Wet Microalgae

2021 ◽  
Author(s):  
Kyoung-Woo Kim ◽  
Hyeon-Jin Jeon ◽  
Jung-Hyeon Kim ◽  
Kyeong-Hwan Kang ◽  
Im-Gyu Byun
Keyword(s):  
Lipid Extraction ◽  
Fermented Food ◽  
Food Wastewater ◽  
Wet Microalgae

Disintegration of wet microalgae biomass with deep-eutectic-solvent-assisted hydrothermal treatment for sustainable lipid extraction

2022 ◽  
Author(s):  
Rui Huang ◽  
Yu He ◽  
Xianrui Yao ◽  
Yujie Yu ◽  
Wenlu Song ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Hydrothermal Treatment ◽  
Lipid Extraction ◽  
Deep Eutectic Solvent ◽  
Microalgae Biomass ◽  
Wet Microalgae

Deep eutectic solvent (DES) with abundant hydrogen bond acceptors and donors was employed to promote disintegration of microalgae biomass with hydrothermal treatment (HTT) for green lipid extraction. The lipid extraction...

scholarly journals Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae

Energies ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3813 ◽  
Author(s):  
Shuai Zhang ◽  
Yuyong Hou ◽  
Zhiyong Liu ◽  
Xiang Ji ◽  
Di Wu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Lipid Extraction ◽  
Extraction Yield ◽  
Biofuel Production ◽  
Transmission Electron ◽  
Cell Wall Disruption ◽  
Dry Cell Weight ◽  
Major Bottleneck ◽  
Wet Microalgae ◽  
Disruption Method

Currently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of Nannochloropsis oceanica IMET1. The results showed that 1.27 mM of hydroxide radical (HO•) was generated under the optimal conditions with 9.1 mM FeSO4 in a 16.4 mA·cm−2 current density for 37.0 min. After the electro-Fenton treatment, the neutral lipid extraction yield of microalgae (~155 mg) increased from 40% to 87.5%, equal to from 12.2% to 26.7% dry cell weight (DCW). In particular, the fatty acid composition remained stable. The cell wall disruption and lipid extraction processes were displayed by the transmission electron microscope (TEM) and fluorescence microscopy (FM) observations, respectively. Meanwhile, the removal efficiency of algal cells reached 85.2% within 2 h after the reaction was terminated. Furthermore, the biomass of the microalgae cultured in the electrolysis wastewater treated with fresh nutrients reached 3 g/L, which is 12-fold higher than that of the initial after 24 days. These finds provided an economic and efficient method for lipid extraction from wet microalgae, which could be easily controlled by current magnitude regulation.

Current Research and Perspectives of Lipid Extraction from Wet Microalgae

2014 ◽  
Vol 1004-1005 ◽  
pp. 873-876
Author(s):  
Xin Miao Xu ◽  
Zhi Xiang Fan ◽  
Ying Shen
Keyword(s):  
Large Scale ◽  
Lipid Extraction ◽  
Extraction Process ◽  
Enzyme Treatment ◽  
Scale Production ◽  
Innovative Methods ◽  
Large Scale Production ◽  
Microalgae Biomass ◽  
High Pressure Homogenizer ◽  
Wet Microalgae

Lipid extraction process claimed about 10-20% of the total cost of microalgal fuels. Cell disruption (bead beater, a high-pressure homogenizer, ultrasoniaction, etc) followed with solvent extraction (hexane, chloroform and methyl, etc) are commonly used in lab-scale experiments. However, it is time consuming and costly to apply these methods in large-scale production. Recently, some innovative methods, such as microwave-assisted process and enzyme treatment are also found effective in lipid extraction from wet microalgae biomass. This article reviewed the current techniques commonly used in lipid extraction from wet microalgae.

scholarly journals Optimization of cell wall disruption and lipid extraction methods by combining different solvents from wet microalgae

2021 ◽  
Author(s):  
Daryush Arabian
Keyword(s):  
Cell Wall ◽  
Lipid Extraction ◽  
Dry Weight ◽  
Extraction Methods ◽  
Biodiesel Production ◽  
Cell Wall Disruption ◽  
Bead Mill ◽  
Wet Microalgae ◽  
Disruption Method ◽  
Chloroform Methanol

Microalgae have emerged as one of the most promising options for biodiesel production over the past few decades. Lipid extraction from microalgae for biodiesel production as a bottleneck of biodiesel production technology was the main purpose of this study. In this study different methods of the cell wall disruption were compared. Then, two methods of ultrasound and bead mill were used as methods of the cell wall disruption. The maximum lipid extracted by ultrasound was 17.10% and by bead mill was 15.16% (based on microalgae biomass dry weight). After the cell wall disruption of microalgae, for lipid extraction, chloroform-methanol solvent combination was used as a high extraction method and hexane-ethanol solvent combination was used as an environmentally friendly method. In this regard, the effect of solvent to biomass ratio, temperature and extraction time was investigated and the optimal results for chloroform-methanol solvent combination were 8 ml/g, 45°C and 60 minutes, respectively, and for hexane-ethanol combination were 6 ml/g, 35◦C and 73 minutes, respectively. Under these optimal conditions, the highest amount of extracted lipid from Chlorella vulgaris with a moisture content of 87.50%, and ultrasound as a cell wall disruption method were obtained 20.39% and 16.41% (based on microalgae dry weight) with a combination of chloroform-methanol solvents and hexane-ethanol respectively. Also the highest extraction rates of 17.63% and 13.85% were obtained for the combination of chloroform-methanol and hexane-ethanol solvents, respectively by bead milling as cell wall disruption method

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