Switchable solvent N, N, N′, N′-tetraethyl-1, 3-propanediamine was dissociated into cationic surfactant to promote cell disruption and lipid extraction from wet microalgae for biodiesel production

Some species of microalgae have high productivity and lipid content, which makes them good candidates for biodiesel production. Biomass separation and cell disruption are important steps in biodiesel production from microalgae. In this work, we explored the fundamentals of electroflotation by alternating current (EFAC) with non-consumable electrodes to simultaneously harvest microalgae and disrupt cells from mixed microalgae obtained from waste stabilization ponds. The harvesting efficiency was evaluated using chlorophyll-a and turbidity, which reached removals of 99% and 95%, respectively, during a batch time of 140 min. Cell disruption was evaluated using lipid extraction, and the best results were achieved with a batch time of 140 min, which resulted in a 14% yield. Therefore, EFAC was shown to be an attractive potential technology for simultaneous microalgal harvesting and cell disruption.

Download Full-text

Comparison of cell disruption techniques prior to lipid extraction from Scenedesmus sp. slurries for biodiesel production using liquid CO2

Green Chemistry ◽

10.1039/c8gc01695j ◽

2018 ◽

Vol 20 (18) ◽

pp. 4330-4338 ◽

Cited By ~ 8

Author(s):

K. J. Viner ◽

P. Champagne ◽

P. G. Jessop

Keyword(s):

Microwave Radiation ◽

Lipid Extraction ◽

Cell Disruption ◽

Biodiesel Production ◽

Liquid Co2

Microwave radiation exhibits the highest potential for releasing lipids from Scenedesmus sp.

Download Full-text

Different Cell Disruption and Lipid Extraction Methods from Microalgae for Biodiesel Production

Microalgae Biotechnology for Development of Biofuel and Wastewater Treatment ◽

10.1007/978-981-13-2264-8_12 ◽

2019 ◽

pp. 265-292 ◽

Cited By ~ 4

Author(s):

Vinod Kumar ◽

Neha Arora ◽

Manisha Nanda ◽

Vikas Pruthi

Keyword(s):

Lipid Extraction ◽

Extraction Methods ◽

Cell Disruption ◽

Biodiesel Production

Download Full-text

Comparison of Nannochloropsis sp. cells disruption between hydrodynamic cavitation and conventional extraction

MATEC Web of Conferences ◽

10.1051/matecconf/201815401023 ◽

2018 ◽

Vol 154 ◽

pp. 01023 ◽

Cited By ~ 1

Author(s):

Martomo Setyawan ◽

Panut Mulyono ◽

Sutijan ◽

Arief Budiman

Keyword(s):

Mass Transfer ◽

Transfer Coefficient ◽

Distribution Coefficient ◽

Mass Transfer Coefficient ◽

Lipid Extraction ◽

Cell Disruption ◽

Hydrodynamic Cavitation ◽

Biodiesel Production ◽

Experimental Result ◽

Conventional Extraction

Biodiesel production from microalgae is one of the solution of the future energy problem, but its production cost is still high. One of the costly stages of this process is the lipid extraction process. It can be reduced by microalgae cell disruption. One of the mechanical method to cell disruption with the lowest energy requirement is hydrodynamic cavitation. This aim of this study is to evaluate the distribution coefficient and the mass transfer coefficient value of lipid extraction of Nannochloropsis sp. assisted by hydrodynamic cavitation and compare with conventional extraction. The hydrodynamic cavitation extraction was done at 34 °C, 1 atm. The conventional extraction was done at 34 °C, 1 atm with stirring speed 260 and 1000 rpm. The experimental result shows that the distribution coefficient dependent on the temperature with the values for 50, 44, 38 and 34 °C were 0.502, 0.394, 0.349, and 0.314 respectively. And it was according to Van’ Hoff equation with the values of ΔH° was 20.718 kJ/mol and ΔS° was 58.05 J/mol/K. The hydrodynamic cavitation extraction was faster than conventional. The mass transfer coefficient values for hydrodynamic cavitation, conventional 260 rpm and 1000 rpm were 7.373, 0.534 and 0.121 1/s respectively.

Download Full-text

Optimization of cell wall disruption and lipid extraction methods by combining different solvents from wet microalgae

10.22541/au.161832642.25069136/v1 ◽

2021 ◽

Author(s):

Daryush Arabian

Keyword(s):

Cell Wall ◽

Lipid Extraction ◽

Dry Weight ◽

Extraction Methods ◽

Biodiesel Production ◽

Cell Wall Disruption ◽

Bead Mill ◽

Wet Microalgae ◽

Disruption Method ◽

Chloroform Methanol

Microalgae have emerged as one of the most promising options for biodiesel production over the past few decades. Lipid extraction from microalgae for biodiesel production as a bottleneck of biodiesel production technology was the main purpose of this study. In this study different methods of the cell wall disruption were compared. Then, two methods of ultrasound and bead mill were used as methods of the cell wall disruption. The maximum lipid extracted by ultrasound was 17.10% and by bead mill was 15.16% (based on microalgae biomass dry weight). After the cell wall disruption of microalgae, for lipid extraction, chloroform-methanol solvent combination was used as a high extraction method and hexane-ethanol solvent combination was used as an environmentally friendly method. In this regard, the effect of solvent to biomass ratio, temperature and extraction time was investigated and the optimal results for chloroform-methanol solvent combination were 8 ml/g, 45°C and 60 minutes, respectively, and for hexane-ethanol combination were 6 ml/g, 35◦C and 73 minutes, respectively. Under these optimal conditions, the highest amount of extracted lipid from Chlorella vulgaris with a moisture content of 87.50%, and ultrasound as a cell wall disruption method were obtained 20.39% and 16.41% (based on microalgae dry weight) with a combination of chloroform-methanol solvents and hexane-ethanol respectively. Also the highest extraction rates of 17.63% and 13.85% were obtained for the combination of chloroform-methanol and hexane-ethanol solvents, respectively by bead milling as cell wall disruption method

Download Full-text

Biodiesel Production of Amphora sp. and Navicula sp. by Different Cell Disruption and Lipid Extraction Methods

Journal of Biobased Materials and Bioenergy ◽

10.1166/jbmb.2015.1563 ◽

2015 ◽

Vol 9 (6) ◽

pp. 588-595

Author(s):

Haifa Chtourou ◽

Ines Dahmen ◽

Fatma Karray ◽

Sami Sayadi ◽

Abdelhafidh Dhouib

Keyword(s):

Lipid Extraction ◽

Extraction Methods ◽

Cell Disruption ◽

Biodiesel Production

Download Full-text

Downstream integration of microalgae harvesting and cell disruption by means of cationic surfactant-decorated Fe3O4 nanoparticles

Green Chemistry ◽

10.1039/c6gc00904b ◽

2016 ◽

Vol 18 (14) ◽

pp. 3981-3989 ◽

Cited By ~ 49

Author(s):

Jung Yoon Seo ◽

Ramasamy Praveenkumar ◽

Bohwa Kim ◽

Jeong-Cheol Seo ◽

Ji-Yeon Park ◽

...

Keyword(s):

Cationic Surfactant ◽

Fe3o4 Nanoparticles ◽

Cationic Surfactants ◽

Lipid Extraction ◽

Cell Disruption ◽

Microalgae Harvesting

The functionalization of cationic surfactants on Fe3O4 nanoparticles serves two roles at the same time: microalgae harvesting and cell disruption for lipid extraction.

Download Full-text

AN INSIGHT ON ALGAL CELL DISRUPTION FOR BIODIESEL PRODUCTION

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i2.22481 ◽

2018 ◽

Vol 11 (2) ◽

pp. 21 ◽

Cited By ~ 2

Author(s):

Aarthy A ◽

Smita Kumari ◽

Prachi Turkar ◽

Sangeetha Subramanian

Keyword(s):

Cell Wall ◽

Enhanced Recovery ◽

Algal Cell ◽

Lipid Extraction ◽

Algal Species ◽

Cost Effective ◽

Downstream Processing ◽

Cell Disruption ◽

Biodiesel Production ◽

Cell Wall Disruption

Objective: This review article deals with the effect that various cell disruption techniques have on the efficiency of lipid extraction. We have reviewed existing algal cell disruption techniques that aid the biodiesel production process.Methods: Current rise in demand for energy has led the researcher to focus on the production of sustainable fuels, among which biodiesel has received greater attention. This is due to its larger lipid content, higher growth rate, larger biomass production, and lower land use. Extraction of lipid from algae (micro and macro) for the production of biodiesel involves numerous downstream processing steps, of which cell wall disruption is a crucial step. Bead milling, high-pressure homogenization, ultra-sonication, freeze-drying, acid treatment, and enzymatic lysis are some methods of cell disruption. The cell disruption technique needs to be optimized based on the structure and biochemical composition of algae.Result: The lipid extraction efficiency varies depending on the algal species and the cell disruption technique used.Conclusion: In-depth research and development of new techniques are required to further enhance the cell disruption of the algal cell wall for the enhanced recovery of lipids. In addition, the operating costs and energy consumption should also be optimized for the cost-effective recovery.

Download Full-text