Triterpenoid saponins from the roots of Rosa rugosa Thunb. as rat intestinal sucrase inhibitors

AbstractPlatycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally. Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus. An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use. The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly. Platycodin D and total polysaccharide content of calli was found higher than that of leaves. While, platycoside E and total polysaccharide content of calli was found lower than that of leaves. Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus. Totally, a candidate gene encoding enzyme involved in converting platycoside E to platycodin D, and putative genes involved in polysaccharide synthesis in P. grandiflorus had been identified. This study will help uncover the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus.

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A chemical procedure for determining the sidedness of the NH2 terminus in a membrane protein. The small intestinal sucrase-isomaltase.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)43779-3 ◽

1983 ◽

Vol 258 (24) ◽

pp. 15114-15119

Author(s):

R Bürgi ◽

J Brunner ◽

G Semenza

Keyword(s):

Membrane Protein ◽

Small Intestinal ◽

Chemical Procedure ◽

Intestinal Sucrase

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Five novel triterpenoid saponins from Hovenia dulcis and their Nrf2 inhibitory activities

Arabian Journal of Chemistry ◽

10.1016/j.arabjc.2021.103292 ◽

2021 ◽

pp. 103292

Author(s):

Wanna Cai ◽

Shengmei Xu ◽

Ting Ma ◽

Xiaoqi Zhang ◽

Bo Liu ◽

...

Keyword(s):

Triterpenoid Saponins ◽

Inhibitory Activities ◽

Hovenia Dulcis

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The Effect of Ripening Stages on the Accumulation of Carotenoids, Polyphenols and Vitamin C in Rosehip Species/Cultivars

Applied Sciences ◽

10.3390/app11156761 ◽

2021 ◽

Vol 11 (15) ◽

pp. 6761

Author(s):

Brigita Medveckienė ◽

Jurgita Kulaitienė ◽

Dovilė Levickienė ◽

Ewelina Hallmann

Keyword(s):

High Performance Liquid Chromatography ◽

Vitamin C ◽

High Performance ◽

Hplc Method ◽

Total Carotenoid ◽

Ripening Stage ◽

Rosa Rugosa ◽

Total Polyphenols ◽

Rosa Canina ◽

Ripening Stages

Our research was aimed at assessing the effect of accumulation of carotenoids, polyphenols, vitamin C and ripening stage in the rosehip fruits of two species—Rosa canina, Rosa rugosa and two cultivar—Rosa rugosa ‘Rubra’ and Rosa rugosa ‘Alba’. The amounts of carotenoids, polyphenols and vitamin C were determined using the high-performance liquid chromatography (HPLC) method. The obtained results showed that the significantly highest amount (107.15 mg 100 g−1) of total carotenoid was determined in the fruits of Rosa canina at ripening Stage V. While results indicated that significant amount of total polyphenols were established at Stages I and II in the Rosa Rugosa ‘Alba’ and Rosa rugosa ‘Rubra’ cultivars (110.34 mg 100 g−1, 107.88 mg 100 g−1 and 103.20 mg 100 g−1 103.39 mg 100 g−1). At ripening Stage I, in the fruits of Rosa rugosa the greatest increases were established in the contents of vitamin C (3036.08 mg 100 g−1).

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Extracts from Pulsatilla patens target cancer-related signaling pathways in HeLa cells

Scientific Reports ◽

10.1038/s41598-021-90136-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Grażyna Łaska ◽

Magdalena Maciejewska-Turska ◽

Elwira Sieniawska ◽

Łukasz Świątek ◽

David S. Pasco ◽

...

Keyword(s):

Signaling Pathways ◽

Hela Cells ◽

Luciferase Reporter ◽

Cervical Cancer Cell Line ◽

Phytochemical Analysis ◽

Root Extract ◽

Triterpenoid Saponins ◽

Cancer Signaling ◽

Methanolic Extracts ◽

Pulsatilla Patens

AbstractThe purpose of this study was to determine if a methanolic extract of the Pulsatilla patens (L.) Mill. can inhibit the progression of cancer through the modulation of cancer-related metabolic signaling pathways. We analyzed a panel of 13 inducible luciferase reporter gene vectors which expression is driven by enhancer elements that bind to specific transcription factors for the evaluation of the activity of cancer signaling pathways. The root extract of P. patens exhibited strong inhibition of several signaling pathways in HeLa cells, a cervical cancer cell line, and was found to be the most potent in inhibiting the activation of Stat3, Smad, AP-1, NF-κB, MYC, Ets, Wnt and Hdghog, at a concentration of 40 µg/mL. The methanolic extracts of P. patens enhanced apoptotic death, deregulated cellular proliferation, differentiation, and progression towards the neoplastic phenotype by altering key signaling molecules required for cell cycle progression. This is the first study to report the influence of Pulsatilla species on cancer signaling pathways. Further, our detailed phytochemical analysis of the methanolic extracts of the P. patens allowed to deduce that compounds, which strongly suppressed the growth and proliferation of HeLa cancer cells were mainly triterpenoid saponins accompanied by phenolic acids.

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