Characterization of a mutant strain of a filamentous fungus Cladosporium phlei for the mass production of the secondary metabolite phleichrome

2011 ◽  
Vol 49 (4) ◽  
pp. 680-683 ◽  
Author(s):  
Min-Hee Yi ◽  
Jung-Ae Kim ◽  
Jung-Mi Kim ◽  
Jin-Ah Park ◽  
Beom-Tae Kim ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Mutant Strain ◽  
Filamentous Fungus ◽  
Mass Production ◽  
Cladosporium Phlei

scholarly journals Molecular Characterization of Xyloglucanase cel74a from Trichoderma reesei

2021 ◽  
Vol 22 (9) ◽  
pp. 4545
Author(s):  
Douglas Christian Borges Lopes ◽  
Cláudia Batista Carraro ◽  
Roberto Nascimento Silva ◽  
Renato Graciano de Paula
Keyword(s):  
Molecular Characterization ◽  
Lignocellulosic Biomass ◽  
Trichoderma Reesei ◽  
Mutant Strain ◽  
Filamentous Fungus ◽  
Significant Contribution ◽  
Genetic Regulation ◽  
Null Mutant ◽  
Carbon Utilization

Background: The filamentous fungus Trichoderma reesei is used on an industrial scale to produce enzymes of biotechnological interest. This fungus has a complex cellulolytic system involved in the degradation of lignocellulosic biomass. However, several aspects related to the regulation of the expression of holocellulolytic genes and the production of cellulases by this fungus are still understood. Methods: Here, we constructed a null mutant strain for the xyloglucanase cel74a gene and performed the characterization of the Δcel74a strain to evaluate the genetic regulation of the holocellulases during sugarcane bagasse (SCB) cultivation. Results: Our results demonstrate that the deletion of xyloglucanase cel74a may impact the regulation of holocellulase expression during SCB cultivation. The expression of cellulases cel7a, cel7b, and cel6a was reduced in Δcel74a strain, while the hemicellulases xyn1 and xyn2 were increased in the presence of SCB. The cel74a mutation also affected the xyloglucan hydrolysis patterns. In addition, CEL74A activity was modulated in the presence of calcium, suggesting that this ion may be required for efficient degradation of xyloglucan. Conclusions: CEL74A affects the regulation of holocellulolytic genes and the efficient degradation of SCB in T. reesei. This data makes a significant contribution to our understanding of the carbon utilization of fungal strains as a whole.

Biochemical characterization of the dicing activity of Dicer-like 2 in the model filamentous fungus Neurospora crassa

2021 ◽  
Vol 146 ◽  
pp. 103488
Author(s):  
Midori Tabara ◽  
Hisashi Koiwa ◽  
Nobuhiro Suzuki ◽  
Toshiyuki Fukuhara
Keyword(s):  
Neurospora Crassa ◽  
Filamentous Fungus ◽  
Biochemical Characterization

Characterization of three multicopper oxidases in the filamentous fungus Podospora anserina : A new role of an ABR1-like protein in fungal development?

2018 ◽  
Vol 116 ◽  
pp. 1-13 ◽  
Author(s):  
Ning Xie ◽  
Gwenaël Ruprich-Robert ◽  
Philippe Silar ◽  
Eric Herbert ◽  
Roselyne Ferrari ◽  
...  
Keyword(s):  
Filamentous Fungus ◽  
Podospora Anserina ◽  
Multicopper Oxidases ◽  
Fungal Development

scholarly journals ISOLATION, CHARACTERIZATION AND SECONDARY METABOLITE ENDOPHYTIC FUNGAL ISOLATE FROM PERONEMA CANESCENS JACK LEAF AND COPTOSAPELTA TOMENTOSA VAL. K. HEYNE ROOT

2019 ◽  
Vol 4 (5) ◽  
pp. 215-225
Author(s):  
Arsyik Ibrahim ◽  
M. Arifuddin ◽  
Wisnu Cahyo P ◽  
Wahyu Widayat ◽  
Mahfuzun Bone
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Secondary Metabolite ◽  
Fungal Isolate ◽  
Chromatography Method ◽  
Fungal Isolates ◽  
Reaction Test ◽  
Layer Chromatography ◽  
Spray Reagents

Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chromatography) method with specific spray reagents. The data of this study were obtained based on the number of endophytic fungal that can be isolated, observing macroscopic and microscopic morphological profiles, chromatogram profile and secondary metabolites of each endophytic fungal isolated. The results showed that endophytic fungal that can be isolated from P. canencens leaves four isolates, and two isolates from C. tomentosa root. Morphological profile macroscopic endophytic fungal of the six isolates showed a greenish-colored colony, white gray, clear black. Microscopic profile of each fungal isolate having spores, sprangiosphora, sporangium, conidia, hyphae and stolon. The identified secondary metabolites are: alkaloids, terpenoids, and flavonoids, and polyphenols.

scholarly journals Chemical Components and Bioactive of Gorgonian Sea Plumes (Genus Rumphella and Hicksonella) from Raijua Islands-East Nusa Tenggara

2015 ◽  
Vol 18 (1) ◽  
Author(s):  
Yunialdi Happynes Teffu ◽  
Ruddy Suwandi ◽  
Nurjanah Nurjanah
Keyword(s):  
Heavy Metal ◽  
Secondary Metabolite ◽  
Bioactive Compounds ◽  
Proximate Analysis ◽  
Chemical Components ◽  
Metal Analysis ◽  
Pharmaceutical Ingredients ◽  
Quality Testing ◽  
Protein Components

Sea plumes is mostly used by people as jewellery and arthritis drug. The aim of this research was to characterize the chemicals and the bioactive compounds of the sea plumes for pharmaceutical ingredients. Characterization of sea plumes was carried out schlerit form identification, proximate analysis, heavy metal analysis using Atomic Absorption Spectrofotometer, radium content using gamma spectrometer, extraction by maceration method for 24 hour and phytochemicals quality testing based on color appearance. The sea plumes was identified as Rumphella and Hicksonella contains the highest protein components. Heavy metal Pb exceed the standar set to National Standar of Indonesia (SNI) and Medicines and Foods Security Agency. Akar bahar containing radium 0.65-0.79 Becquerel/kilogram, highest yield using methanol and containing secondary metabolite which targeted as bioactive compounds alkaloids, flavonoids, phenols hydroquinone, steroids, triterpenoids and saponins. Keywords : Hicksonella, secondary metabolite, radium, Rumphella, sklerit

Enzymatic characterization of agmatinase (AGM-1) from the filamentous fungus Neurospora crassa

2021 ◽  
pp. 103634
Author(s):  
Luis L. Pérez-Mozqueda ◽  
Rafael Vazquez-Duhalt ◽  
Ernestina Castro-Longoria
Keyword(s):  
Neurospora Crassa ◽  
Filamentous Fungus ◽  
Enzymatic Characterization
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