Genetic modification of Gossypium arboreum universal stress protein (GUSP1) improves drought tolerance in transgenic cotton (Gossypium hirsutum)

2021 ◽  
Vol 27 (8) ◽  
pp. 1779-1794
Author(s):  
Sameera Hassan ◽  
Aftab Ahmad ◽  
Fatima Batool ◽  
Bushra Rashid ◽  
Tayyab Husnain
Keyword(s):  
Drought Tolerance ◽  
Gossypium Hirsutum ◽  
Genetic Modification ◽  
Stress Protein ◽  
Transgenic Cotton ◽  
Gossypium Arboreum ◽  
Universal Stress Protein

Universal stress protein in Malus sieversii confers enhanced drought tolerance

2019 ◽  
Vol 132 (6) ◽  
pp. 825-837
Author(s):  
Meiling Yang ◽  
Shiyou Che ◽  
Yunxiu Zhang ◽  
Hongbin Wang ◽  
Tao Wei ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Stress Protein ◽  
Universal Stress Protein ◽  
Malus Sieversii

Comparative evaluation of Gossypium arboreum L. and Gossypium hirsutum L. genotypes for drought tolerance

2019 ◽  
Vol 17 (6) ◽  
pp. 506-513
Author(s):  
Muhammad Iqbal ◽  
Mueen Alam Khan ◽  
Waqas Shafqat Chattha ◽  
Khalid Abdullah ◽  
Asif Majeed
Keyword(s):  
Drought Tolerance ◽  
Gossypium Hirsutum ◽  
Negative Impact ◽  
Primary Root ◽  
Dry Weight ◽  
Fibre Strength ◽  
Gossypium Arboreum ◽  
Cotton Production ◽  
Chlorophyll Contents ◽  
Cotton Species

AbstractDrought stress negatively affects the cotton production all over the world. The negative impact of drought varies for different species due to some morphological and root attributes that help some species to better stand under drought. But the extent of disturbance varies for different cotton species. To find out such variation, two cotton species (Gossypium hirsutum and Gossypium arboreum) were studied under normal and drought conditions for 2 years. Two genotypes for each species were included, i.e. PC-1 and COMILLA (G. arboreum) and IUB-13 and IUB-65 (G. hirsutum). The experiment was laid out under a completely randomized design following factorial arrangement. Genotype × treatment × year interaction of cotton genotypes was studied for different root, morphological, physiological and fibre-related traits. Traits such as above ground dry biomass, above ground fresh biomass, chlorophyll contents, leaf area, seed cotton yield, sympodial branches/plant, fibre strength and ginning out-turn were higher in G. hirsutum genotypes as compared to G. arboreum genotypes. However less reduction under drought in all above mentioned traits was recorded for G. arboreum, than G. hirsutum. Furthermore, root traits; primary root length, lateral root numbers, root fresh weight and root dry weight were enriched under drought condition in G. arboreum genotypes than in G. hirsutum genotypes, which is a clear manifestation of higher drought tolerance ability in G. arboreum genotypes transferrable to G. hirsutum genotypes through interspecific crossing or other means.

scholarly journals SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato

2012 ◽  
Vol 63 (15) ◽  
pp. 5593-5606 ◽  
Author(s):  
Rachid Loukehaich ◽  
Taotao Wang ◽  
Bo Ouyang ◽  
Khurram Ziaf ◽  
Hanxia Li ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Stress Protein ◽  
Universal Stress Protein

scholarly journals Mutant Gossypium universal stress protein-2 (GUSP-2) gene confers resistance to various abiotic stresses in E. coli BL-21 and CIM-496-Gossypium hirsutum

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Muhammad Nadeem Hafeez ◽  
Mohsin Ahmad Khan ◽  
Bilal Sarwar ◽  
Sameera Hassan ◽  
Qurban Ali ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Stress Protein ◽  
In Silico Analysis ◽  
Gene Transcript ◽  
Adaptive Mechanism ◽  
Bacterial Cells ◽  
Universal Stress Protein ◽  
E Coli

AbstractGossypium arboreum is considered a rich source of stress-responsive genes and the EST database revealed that most of its genes are uncharacterized. The full-length Gossypium universal stress protein-2 (GUSP-2) gene (510 bp) was cloned in E. coli and Gossypium hirsutum, characterized and point mutated at three positions, 352–354, Lysine to proline (M1-usp-2) & 214–216, aspartic acid to serine (M2-usp-2) & 145–147, Lysine to Threonine (M3-usp-2) to study its role in abiotic stress tolerance. It was found that heterologous expression of one mutant (M1-usp-2) provided enhanced tolerance against salt and osmotic stresses, recombinant cells have higher growth up to 10-5dilution in spot assay as compared to cells expressing W-usp-2 (wild type GUSP-2), M2-usp-2 and M3-usp-2 genes. M1-usp-2 gene transcript profiling exhibited significant expression (8.7 fold) in CIM-496-Gossypium hirsutum transgenic plants and enhance drought tolerance. However, little tolerance against heat and cold stresses in bacterial cells was observed. The results from our study concluded that the activity of GUSP-2 was enhanced in M1-usp-2 but wipe out in M2-usp-2 and M3-usp-2 response remained almost parallel to W-usp-2. Further, it was predicted through in silico analysis that M1-usp-2, W-usp-2 and M3-usp-2 may be directly involved in stress tolerance or function as a signaling molecule to activate the stress adaptive mechanism. However, further investigation will be required to ascertain its role in the adaptive mechanism of stress tolerance.

Spatial and temporal regulation of a soybean (Glycine max) lectin promoter in transgenic cotton (Gossypium hirsutum)

2002 ◽  
Vol 29 (7) ◽  
pp. 835 ◽  
Author(s):  
Belinda J. Townsend ◽  
Danny J. Llewellyn
Keyword(s):  
Glycine Max ◽  
Gossypium Hirsutum ◽  
Embryo Development ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Transgenic Cotton ◽  
Gus Activity ◽  
Mature Seed ◽  
Lectin Gene ◽  
Lectin Promoter

The activity of a soybean (Glycine max L. Merrill) lectin gene promoter was investigated in transgenic cotton plants (Gossypium hirsutum L.) with the view to using this promoter for the seed-specific alteration of gossypol, a secondary metabolite in cotton that has adverse effects on the nutritional value of cottonseed products like oil and protein-rich meal. Agrobacterium-mediated transformation generated stable transformants containing a construct with the lectin promoter fused to the β-glucuronidase reporter gene (pLeGUS). Fluorometric GUS assays and northern hybridization detected strong promoter activity during embryo development. GUS activity in developing embryos was detected as early as 10 d post-anthesis (dpa), peaking late in embryo maturation. Enzyme activity persisted in imbibed mature seed, and negligible activity remained detectable in the roots and cotyledons of 7-d-old seedlings. No GUS activity was detected in leaves and squares of mature plants. GUS transcripts increased during embryo development to peak about 35 dpa, declining to a low level in imbibed mature seed. No transcripts were detected in roots, cotyledons, leaves or squares. Histochemical GUS activity staining indicated promoter activity in all cells of the cotyledons, including the flattened cells of the gossypol glands, the presumed site of synthesis of gossypol. This study concluded that the soybean lectin gene promoter is a useful tool for the seed-specific expression of transgenes in cotton.

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