Cloning, antibody production, expression and cellular localization of universal stress protein gene (USP1-GFP) in transgenic cotton

2017 ◽  
Author(s):  
Sameera Hassan ◽  
Tahir Rehman Samiullah ◽  
Mahmood ur Rahman Ansari ◽  
Bushra Rashid ◽  
Tayyab Husnain
Keyword(s):  
Antibody Production ◽  
Cellular Localization ◽  
Protein Gene ◽  
Stress Protein ◽  
Transgenic Cotton ◽  
Universal Stress Protein

scholarly journals CLONING AND EXPRESSION OF UNIVERSAL STRESS PROTEIN 2 (USP2) GENE IN ESCHERICHIA COLI

2021 ◽  
Vol 2021 (1) ◽  
Author(s):  
A Akram ◽  
K Arshad ◽  
MN Hafeez
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Protein Gene ◽  
Abiotic Stress Tolerance ◽  
Stress Protein ◽  
Crop Plants ◽  
Cloning And Expression ◽  
Major Type ◽  
Transgenic Crop ◽  
Universal Stress Protein

Different types of abiotic stresses inhibit the normal growth of plants by changing their physical biochemical, morphological, and molecular traits. It links to the polygenic traits, which is controlled with the help of different genes, due to this polygenetic the manipulation of foreign genetic makeup is very difficult. Drought stress is the very major type of threat to reduce the yield of cash crops in Pakistan and as well as in all over the world. Gene manipulation is the solution to face this problem by producing genetically modified crop plants that have the ability to survive in drought conditions. Universal stress protein gene has been already identified in bacteria which showed its response under stressed conditions, by manipulation of universal stress protein gene. It was found from our study that the bacterial cells transformed with the USP2 gene isolated from cotton induced abiotic stress tolerance under heat, osmotic, and salt stress. It was suggested from our findings that the USP2 gene could be used to produce abiotic stress tolerance transgenic crop plants to enhance crop plant yield and quality.

The bacterial universal stress protein: function and regulation

2003 ◽  
Vol 6 (2) ◽  
pp. 140-145 ◽  
Author(s):  
Kristian Kvint ◽  
Laurence Nachin ◽  
Alfredo Diez ◽  
Thomas Nyström
Keyword(s):  
Protein Function ◽  
Stress Protein ◽  
Universal Stress Protein

scholarly journals Universal stress protein Rv2624c alters abundance of arginine and enhances intracellular survival by ATP binding in mycobacteria

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Qiong Jia ◽  
Xinling Hu ◽  
Dawei Shi ◽  
Yan Zhang ◽  
Meihao Sun ◽  
...  
Keyword(s):  
Mycobacterium Smegmatis ◽  
Metabolic Pathways ◽  
Biochemical Analysis ◽  
Stress Proteins ◽  
Stress Protein ◽  
Human Monocyte ◽  
Dependent Manner ◽  
Proline Metabolism ◽  
Universal Stress Protein ◽  
Induced Proteins

Abstract The universal stress protein family is a family of stress-induced proteins. Universal stress proteins affect latency and antibiotic resistance in mycobacteria. Here, we showed that Mycobacterium smegmatis overexpressing M. tuberculosis universal stress protein Rv2624c exhibits increased survival in human monocyte THP-1 cells. Transcriptome analysis suggested that Rv2624c affects histidine metabolism, and arginine and proline metabolism. LC-MS/MS analysis showed that Rv2624c affects the abundance of arginine, a modulator of both mycobacteria and infected THP-1 cells. Biochemical analysis showed that Rv2624c is a nucleotide-binding universal stress protein, and an Rv2624c mutant incapable of binding ATP abrogated the growth advantage in THP-1 cells. Rv2624c may therefore modulate metabolic pathways in an ATP-dependent manner, changing the abundance of arginine and thus increasing survival in THP-1 cells.

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