Transcriptional activities of medaka Oryzias latipes peroxisome proliferator-activated receptors and their gene expression profiles at different temperatures

2009 ◽  
Vol 76 (1) ◽  
pp. 167-175 ◽  
Author(s):  
Hidehiro Kondo ◽  
Ryohei Misaki ◽  
Shugo Watabe
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Oryzias Latipes ◽  
Peroxisome Proliferator ◽  
Different Temperatures ◽  
Peroxisome Proliferator Activated Receptors

scholarly journals Transcriptomic Profiles in Zebrafish Liver Permit the Discrimination of Surface Water with Pollution Gradient and Different Discharges

2018 ◽  
Vol 15 (8) ◽  
pp. 1648 ◽  
Author(s):  
Zhou Zhang ◽  
Wei Liu ◽  
Yuanyuan Qu ◽  
Xie Quan ◽  
Ping Zeng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Surface Water ◽  
Expression Profiles ◽  
Oxygen Demand ◽  
Gene Expression Profiles ◽  
Peroxisome Proliferator ◽  
Pollution Gradient ◽  
Aquatic Vertebrates ◽  
Hun River ◽  
Peroxisome Proliferator Activated Receptors

The present study aims to evaluate the potential of transcriptomic profiles in evaluating the impacts of complex mixtures of pollutants at environmentally relevant concentrations on aquatic vertebrates. The changes in gene expression were determined using microarray in the liver of male zebrafish (Danio rerio) exposed to surface water collected from selected locations on the Hun River, China. The numbers of differentially expressed genes (DEGs) in each treatment ranged from 728 to 3292, which were positively correlated with chemical oxygen demand (COD). Predominant transcriptomic responses included peroxisome proliferator-activated receptors (PPAR) signaling and steroid biosynthesis. Key pathways in immune system were also affected. Notably, two human diseases related pathways, insulin resistance and Salmonella infection were enriched. Clustering analysis and principle component analysis with DEGs differentiated the upstream and downstream site of Shenyang City, and the mainstream and the tributary sites near the junction. Comparison the gene expression profiles of zebrafish exposed to river surface water with those to individual chemicals found higher similarity of the river water with estradiol than several other organic pollutants and metals. Results suggested that the transcriptomic profiles of zebrafish is promising in differentiating surface water with pollution gradient and different discharges and in providing valuable information to support discharge management.

Functional genomic characterization of delipidation elicited by trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) in a polygenic obese line of mice

2005 ◽  
Vol 21 (3) ◽  
pp. 351-361 ◽  
Author(s):  
Ralph L. House ◽  
Joseph P. Cassady ◽  
Eugene J. Eisen ◽  
Thomas E. Eling ◽  
Jennifer B. Collins ◽  
...  
Keyword(s):  
Gene Expression ◽  
Linoleic Acid ◽  
Glucose Transporter ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Liver Weight ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Adult Mice ◽  
Brown Adipose

Gene expression was measured during t10c12-CLA-induced body fat reduction in a polygenic obese line of mice. Adult mice ( n = 185) were allotted to a 2 × 2 factorial experiment consisting of either nonobese (ICR-control) or obese (M16-selected) mice fed a 7% fat, purified diet containing either 1% linoleic acid (LA) or 1% t10c12-CLA. Body weight (BW) by day 14 was 12% lower in CLA- compared with LA-fed mice ( P < 0.0001). By day 14, t10c12-CLA reduced weights of epididymal, mesenteric, and brown adipose tissues, as a percentage of BW, in both lines by 30, 27, and 58%, respectively, and increased liver weight/BW by 34% ( P < 0.0001). Total RNA was isolated and pooled (4 pools per tissue per day) from epididymal adipose ( days 5 and 14) of the obese mice to analyze gene expression profiles using Agilent mouse oligo microarray slides representing >20,000 genes. Numbers of genes differentially expressed by greater than or equal to twofold in epididymal adipose ( days 5 and 14) were 29 and 125, respectively. It was concluded that, in adipose tissue, CLA increased expression of uncoupling proteins (1 and 2), carnitine palmitoyltransferase system, tumor necrosis factor-α ( P < 0.05), and caspase-3 but decreased expression of peroxisome proliferator-activated receptor-γ, glucose transporter-4, perilipin, caveolin-1, adiponectin, resistin, and Bcl-2 ( P < 0.01). In conclusion, this experiment has revealed candidate genes that will be useful in elucidating mechanisms of adipose delipidation.

Gene expression profiles in the testis associated with testis–ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol

Chemosphere ◽  
2012 ◽  
Vol 87 (7) ◽  
pp. 668-674 ◽  
Author(s):  
Ikumi Hirakawa ◽  
Shinichi Miyagawa ◽  
Yoshinao Katsu ◽  
Yoshihiro Kagami ◽  
Norihisa Tatarazako ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Oryzias Latipes ◽  
Japanese Medaka

scholarly journals Comparison of gene expression profiles between human and mouse monocyte subsets

Blood ◽  
2010 ◽  
Vol 115 (3) ◽  
pp. e10-e19 ◽  
Author(s):  
Molly A. Ingersoll ◽  
Rainer Spanbroek ◽  
Claudio Lottaz ◽  
Emmanuel L. Gautier ◽  
Marion Frankenberger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Surface Protein ◽  
Human Monocyte ◽  
Peroxisome Proliferator ◽  
Cell Surface Protein ◽  
Monocyte Subsets ◽  
Peroxisome Proliferator Activated Receptor ◽  
Human And Mouse

Abstract Blood of both humans and mice contains 2 main monocyte subsets. Here, we investigated the extent of their similarity using a microarray approach. Approximately 270 genes in humans and 550 genes in mice were differentially expressed between subsets by 2-fold or more. More than 130 of these gene expression differences were conserved between mouse and human monocyte subsets. We confirmed numerous of these differences at the cell surface protein level. Despite overall conservation, some molecules were conversely expressed between the 2 species' subsets, including CD36, CD9, and TREM-1. Other differences included a prominent peroxisome proliferator-activated receptor γ (PPARγ) signature in mouse monocytes, which is absent in humans, and strikingly opposed patterns of receptors involved in uptake of apoptotic cells and other phagocytic cargo between human and mouse monocyte subsets. Thus, whereas human and mouse monocyte subsets are far more broadly conserved than currently recognized, important differences between the species deserve consideration when models of human disease are studied in mice.

Alteration of gene expression profiles in the brain of Japanese medaka (Oryzias latipes) exposed to KC-400 or PCB126

2008 ◽  
Vol 57 (6-12) ◽  
pp. 460-466 ◽  
Author(s):  
Kei Nakayama ◽  
Naomi Sei ◽  
Yuji Oshima ◽  
Kosuke Tashiro ◽  
Yohei Shimasaki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Oryzias Latipes ◽  
Japanese Medaka ◽  
The Brain

scholarly journals Gene Expression Profiles Induced by a Novel Selective Peroxisome Proliferator-Activated Receptor α Modulator (SPPARMα) Pemafibrate

2019 ◽  
Vol 20 (22) ◽  
pp. 5682 ◽  
Author(s):  
Yusuke Sasaki ◽  
Sana Raza-Iqbal ◽  
Toshiya Tanaka ◽  
Kentaro Murakami ◽  
Motonobu Anai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Vascular Endothelial Cells ◽  
Expression Profiles ◽  
Microvascular Complications ◽  
Density Lipoprotein ◽  
Gene Expression Profiles ◽  
Atherogenic Dyslipidemia ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor

Pemafibrate is the first clinically-available selective peroxisome proliferator-activated receptor α modulator (SPPARMα) that has been shown to effectively improve hypertriglyceridemia and low high-density lipoprotein cholesterol (HDL-C) levels. Global gene expression analysis reveals that the activation of PPARα by pemafibrate induces fatty acid (FA) uptake, binding, and mitochondrial or peroxisomal oxidation as well as ketogenesis in mouse liver. Pemafibrate most profoundly induces HMGCS2 and PDK4, which regulate the rate-limiting step of ketogenesis and glucose oxidation, respectively, compared to other fatty acid metabolic genes in human hepatocytes. This suggests that PPARα plays a crucial role in nutrient flux in the human liver. Additionally, pemafibrate induces clinically favorable genes, such as ABCA1, FGF21, and VLDLR. Furthermore, pemafibrate shows anti-inflammatory effects in vascular endothelial cells. Pemafibrate is predicted to exhibit beneficial effects in patients with atherogenic dyslipidemia and diabetic microvascular complications.

scholarly journals Fatty degeneration and wnt10b expression in the supraspinatus muscle after surgical repair of torn rotator cuff tendon

2019 ◽  
Vol 27 (3) ◽  
pp. 230949901986481
Author(s):  
Yoshiyuki Kuwahara ◽  
Koshi N Kishimoto ◽  
Yoshiaki Itoigawa ◽  
Hiroshi Okuno ◽  
Taku Hatta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rotator Cuff ◽  
Expression Profiles ◽  
Rabbit Model ◽  
Central Area ◽  
Gene Expression Profiles ◽  
Fatty Degeneration ◽  
Peroxisome Proliferator ◽  
Messenger Ribonucleic Acid ◽  
Oil Red O

Purpose: In the torn rotator cuff muscles, decreased expression of wnt10b prior to elevation of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα) has previously been reported. The purpose of this study is to elucidate the expression profiles of these adipogenesis-related genes after rotator cuff detachment and reattachment in a rabbit model. Methods: We investigated gene expression profiles of PPARγ, C/EBPα, and wnt10b in different parts of rabbit supraspinatus (SSP) muscle after tendon detachment ( n = 6 for each time point). In addition, we assessed expression of the same genes after SSP reattachment with different intervals from initial detachment ( n = 6). Fatty degeneration of the SSP muscle was examined by Oil red-O staining. Gene expression profiles were examined by quantitative real-time polymerase chain reaction. Results: After SSP detachment, Oil red-O-positive oil deposits increased after 3 weeks. In the SSP reattachment model, numerous Oil red-O-positive cells were present at 5-week reattachment, following 2- and 3-week detachment. PPARγ and C/EBPα messenger ribonucleic acid expression exhibited a significant increase at 2 and 3 weeks after SSP detachment and remained increased at 5-week reattachment after 2- and 3-week detachment. A decreased expression of wnt10b was observed from 1 week after SSP detachment. Expression of wnt10b was recovered not in the central area of the SSP muscle but in the periphery after reattachment. Adipogenic change was not observed when SSP tendon was reattached after 1-week detachment. Conclusions: These results may suggest that once the adipogenic transcription factors, PPARγ and C/EBPα, were elevated, repair surgery after rotator cuff tear could not prevent the emergence of fat in the SSP muscle.

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