Probiotic Properties and Cellular Antioxidant Activity of Lactobacillus plantarum MA2 Isolated from Tibetan Kefir Grains

2017 ◽  
Vol 10 (3) ◽  
pp. 523-533 ◽  
Author(s):  
Wei Tang ◽  
Chao Li ◽  
Zengguo He ◽  
Fen Pan ◽  
Shuo Pan ◽  
...  
2021 ◽  
pp. 8-12
Author(s):  
Елизавета Владимировна Залисская

Полноценное и здоровое питание является неотъемлемым фактором, прямо влияющим на развитие организма и общего здоровья человека. В мире все больший интерес представляет разработка и производство функциональных продуктов питания. Среди них сегмент молочных продуктов считается одним из самых быстрорастущих и перспективных. Особый интерес для научного сообщества представляют природные ассоциации, нашедшие применение в пищевой промышленности. Кефирный грибок - естественная спонтанная ассоциация нескольких видов микроорганизмов, широко используемая для производства кисломолочного продукта кефир. В состав кефира входят более 50 видов различных молочнокислых бактерий и дрожжей, что отличает его от других пробиотических продуктов. Исследователи, занимающиеся изучением кефира, открывают новые, ранее неизвестные его свойства. Большое количество исследований проводится по пробиотическим свойствам кефира, в частности широко изучаются его антимикробные, противоопухолевые, иммуномодулирующие, противовоспалительные и ранозаживляющие действия. Подобные пробиотические свойства кефира связывают в основном с присутствием лактобацилл в составе кефирных грибков. Также в ряде исследований указывается, что кефир обладает более выраженной антиоксидантной активностью, чем известный антиоксидант витамин Е. В статье описаны такие механизмы антиоксидантной активности бактерий, входящих в состав кефира, как ферментативная защита клеток, хелатирующая способность лактобактерий и иные механизмы, нейтрализующие действия свободных радикалов. Также собраны и представлены наиболее популярные методы определения антиоксидантной активности, такие как ABTS, DPPH, ORAC, FRAP. Приведены результаты исследований кефира, приготовленного с использованием молока различных сельскохозяйственных животных. A complete and healthy diet is an integral factor that directly affects the development of the body and general human health. The development and production of functional food products are of increasing interest in the world. Among them, the segment of dairy products is considered one of the fastest-growing and most promising. Natural associations that have found application in the food industry are of particular interest to the scientific community. Kefir grains are a natural spontaneous association of several types of microorganisms, widely used for the production of the fermented milk product kefir. Kefir contains more than 50 different species of lactic acid bacteria and yeast, which distinguishes it from other probiotic products. Researchers studying kefir are finding new and hitherto unknown properties. A large number of studies are carried out on the probiotic properties of kefir, in particular, its antimicrobial, antitumor, immunomodulating, anti-inflammatory, and wound-healing effects are widely studied. Such probiotic properties of kefir are mainly associated with the presence of lactobacilli in kefir grains. Several studies indicate that kefir has a more pronounced antioxidant activity than the well-known antioxidant vitamin E. The article describes mechanisms of the antioxidant activity of bacteria that make up kefir, such as enzymatic antioxidant protection of cells, the chelating ability of lactobacilli, and other mechanisms of antioxidant activity. Also collected and presented the most popular methods for determining antioxidant activity, such as ABTS, DPPH, ORAC, FRAP. The results of studies of kefir prepared using milk from various farm animals are presented.


Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 544 ◽  
Author(s):  
Łukasz Łopusiewicz ◽  
Emilia Drozłowska ◽  
Paulina Siedlecka ◽  
Monika Mężyńska ◽  
Artur Bartkowiak ◽  
...  

Flaxseed oil cake (FOC) was evaluated as a potential substrate for the production of a novel kefir-like fermented beverage. Three variants containing 5%, 10%, and 15% (w/w) of FOC were inoculated with kefir grains and incubated at 25 °C for 24 h. After processing, beverages were stored in refrigerated conditions (6 °C) for 21 days. Changes in microbial population, pH, acidity, levels of proteins, polyphenolics, flavonoids, ascorbic acid, and reducing sugars were estimated. Additionally, viscosity, firmness, color, and antioxidant properties were determined. Results showed that lactic acid bacteria as well as yeast were capable of growing well in the FOC without any supplementation. During refrigerated storage, the viability of the microorganisms were over the recommended minimum level for kefir products. As a result of fermentation, the beverages showed excellent antioxidant activity. Because of the functional characteristics conferred to the FOC beverages, the use of kefir grains showed adequate potential for the industrial application. Therefore, this beverages could be used as a new, non-dairy vehicle for beneficial microflora consumption, especially by vegans and lactose-intolerant consumers.


2013 ◽  
Vol 96 (5) ◽  
pp. 2816-2825 ◽  
Author(s):  
Ying Huang ◽  
Fei Wu ◽  
Xiaojun Wang ◽  
Yujie Sui ◽  
Longfei Yang ◽  
...  

2014 ◽  
Vol 158 ◽  
pp. 385-391 ◽  
Author(s):  
Dongxiao Su ◽  
Huihui Ti ◽  
Ruifen Zhang ◽  
Mingwei Zhang ◽  
Zhengchen Wei ◽  
...  

2018 ◽  
Vol 26 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Barbara Kazuń ◽  
Krzysztof Kazuń ◽  
Joanna Żylińska ◽  
Andrzej K. Siwicki

Abstract The presence of lactic acid bacteria (LAB) favors the stabilization of intestinal flora, facilitates digestion, improves the assimilability of fodder, and has an immunomodulatory effect on the immune system. According to current research, the application of LAB following antibiotic treatment prevents the development of opportunistic bacteria inhabiting the digestive tract. In the study the potential probiotic properties of Lactobacillus plantarum strains, which can be administered as an alternative to antibiotic treatment in aquaculture, were investigated under in vitro conditions. The strains of L. plantarum were characterized for important properties such as the ability to grow in the presence of 10% fish bile, a tolerance of low pH, and antagonism to pathogens dangerous for fish such as Aeromonas salmonicida and Pseudomonas fluorescens; therefore, they meeting the criteria for strains with probiotic properties. In view of currently increasing resistance to antibiotics and a decrease of their efficiency, probiotic bacteria can serve to support immunity to infections in the future.


ACS Omega ◽  
2021 ◽  
Author(s):  
Shen Song ◽  
Xiaoyuan Liu ◽  
Baotang Zhao ◽  
Mohamed Aamer Abubaker ◽  
Yulong Huang ◽  
...  

2017 ◽  
Vol 221 ◽  
pp. 1642-1649 ◽  
Author(s):  
Wei Tang ◽  
Zhuqing Xing ◽  
Chao Li ◽  
Jinju Wang ◽  
Yanping Wang

2010 ◽  
Vol 61 (6) ◽  
pp. 584-589 ◽  
Author(s):  
Willem A. Prins ◽  
Marlie Botha ◽  
Marelize Botes ◽  
Michele de Kwaadsteniet ◽  
Akihito Endo ◽  
...  

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