scholarly journals A serological study of Leishmania infantum in dogs of Khorasan Razavi province, Iran

2012 ◽  
Vol 37 (2) ◽  
pp. 189-191 ◽  
Author(s):  
S. Sabzevari ◽  
G. R. Razmi ◽  
A. Naghibi ◽  
J. Khoshnegah
Keyword(s):  
Leishmania Infantum ◽  
Serological Study

scholarly journals Canine Leishmaniosis in Greece: An Updated Countrywide Serological Study and Associated Risk Factors

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1129
Author(s):  
Isaia Symeonidou ◽  
Athanasios Angelou ◽  
Alexandros Theodoridis ◽  
Georgios Sioutas ◽  
Elias Papadopoulos
Keyword(s):  
Risk Factors ◽  
Leishmania Infantum ◽  
Current Knowledge ◽  
Annual Rainfall ◽  
Canine Leishmaniosis ◽  
Serological Study ◽  
Effective Prevention ◽  
Test Kit ◽  
Seropositive Animal ◽  
Canine Population

Canine leishmaniosis (Leishmania infantum) is a zoonotic disease that affects dogs worldwide. Greece is enzootic for this disease, and updated data for its current distribution are of major importance. The aim of this cross-sectional serological study was primarily to update the current knowledge of Leishmania infantum seropositivity status within the asymptomatic Greek canine population and, furthermore, to assess the possible climatological and other risk factors. In total, sera of 1265 asymptomatic dogs were collected from all prefectures of the country. A questionnaire that included all individual dog information was completed for all animals. The Speed Leish K® canine Leishmania antibody test kit (BVT Groupe Virbac, France) was employed. Potential risk factors were evaluated utilizing logistic regression models. Overall, 13.8% (n = 175) of the sampled dogs were seropositive to Leishmania infantum originating from all geographical departments of the country, whereas most prefectures had at least one seropositive animal. Outdoor living, high mean humidity, low mean wind speed and high total annual rainfall were found to increase the seropositivity status against the parasite. Conclusively, Leishmania infantum remains a common parasite challenge in the asymptomatic canine population of Greece, and therefore, its early diagnosis and effective prevention are significant in the country.

Leishmanicidal Activity of Plant Extracts from Sefrou, a Moroccan Focus of Leishmaniasis, against Various Leishmania Parasites in the Promastigote Stage

2018 ◽  
Vol 17 (2) ◽  
pp. 83-89 ◽  
Author(s):  
I. Zeouk ◽  
A. Et-Touys ◽  
M. Balouiri ◽  
H. Fellah ◽  
A. El Ouali Lalami ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Plant Extracts ◽  
Leishmania Infantum ◽  
Leishmania Major ◽  
Local Population ◽  
Public Health Problem ◽  
World Health ◽  
Total Phenolic ◽  
Leishmania Tropica ◽  
Cistus Salviifolius

According to the World Health Organization, leishmaniasis remains a major worldwide public health problem. The province of Sefrou located in the center of Morocco is a focus of cutaneous leishmaniasis. The present study aims at evaluating the antileishmanial potential of Berberis sp.,Crataegus oxyacantha, Cistus salviifolius, Ephedra altissima and Lavandula dentatafrequently used by the local population. Methanolic extracts were tested against the promastigote form ofLeishmania tropica, Leishmania majorandLeishmania infantumusing tetrazolium-based colorimetric (MTT) assay. The total phenol and flavonoids content of all extracts were determined using the Folin–Ciocalteu reagent, aluminum chloride, and potassium acetate solutions respectively. The plant extracts exhibited antileishmanial activity with variability depending on the tested strain and the plant species compared to Glucantime® used as control (IC50 (the half maximal inhibitory concentration) > 1,000 μg/mL). The best inhibition was observed with Berberis sp., againstLeishmania major(IC50 = 394.40 ± 3.02 μg/ml), andEphedra altissima(reported for the first time) againstLeishmania infantum(IC50 = 490.84 ± 3.15 μg/mL).Leishmania tropicahas shown the same sensitivity behavior toward the five extracts (in average IC50 = 540 ± 11.20 μg/mL). The total phenolic content was higher forCrataegus oxyacanthaandCistus salviifolius(140.67 ± 3.17 μg eq Gallic Acid (GA)/ mg of Extract (E) and 133.83 ± 9.03 μg eq GA/mg of E respectively), while flavonoid was higher forCistus salviifoliusandLavandula dentata(57.92 ± 2.46 μg eq Quercetin (Que)/ mg of Extract (E) and 41.53 ± 1.74 μg eq Que/mg of E). All the tested extracts present some promising aspects that may cure cutaneous leishmaniasis in the center of Morocco; further bioguided assays are needed to isolate the fractions and the bioactive molecule.

Novel Scaffolds for Leishmania infantum Trypanothione Reductase Inhibitors Derived from Brazilian Natural Products Biodiversity

2021 ◽  
Vol 18 (4) ◽  
pp. 398-418
Author(s):  
Vinícius Guimarães da Paixão ◽  
Samuel Silva da Rocha Pita
Keyword(s):  
Natural Products ◽  
Leishmania Infantum ◽  
In Vitro Assays ◽  
Current Therapy ◽  
Pharmacokinetic Analysis ◽  
Trypanothione Reductase ◽  
Resistant Species ◽  
Reductase Inhibitors ◽  
Thiol Redox

Background: Leishmania infantum causes the most lethal form of Leishmaniasis: Visceral leishmaniasis. Current therapy for this disease is related to the development of drug-resistant species and toxicity. Trypanothione Reductase (LiTR), a validated target for the drug discovery process, is involved with parasites' thiol-redox metabolism. Objective: In this study, through Virtual Screening employing two distinct Natural Products Brazilian databases, we aimed to identify novel inhibitor scaffolds against LiTR. Results: Thus, the “top 10” LiTR-ligand energies have been selected and their interaction profiles into LiTR sites through the AuPosSOM server have been verified. Finally, Pred-hERG, Aggregator Advisor, FAF-DRUGS, pkCSM and DataWarrior were employed and their results allowed us to evaluate, respectively, the cardiotoxicity, aggregation capacity, presence of false-positive compounds (PAINS) and their toxicities. Conclusion: Three molecules that overcame the in silico pharmacokinetic analysis and have a good interaction with LiTR, were chosen to use in vitro assays hoping that our computational results reported here would aid the development of new anti-leishmanial compounds.

A Serological Study on Bovine Leukemia Virus Infection in Ten Provinces of Iran between 2010 and 2012

2012 ◽  
Vol 6 (3) ◽  
pp. 1-7 ◽  
Author(s):  
M Kazemimanesh ◽  
O Madadgar ◽  
MR Mahzoonieh ◽  
T Zahraei-Salehi ◽  
F Steinbach ◽  
...  
Keyword(s):  
Virus Infection ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Leukemia Virus Infection ◽  
Serological Study

scholarly journals MYCOLOGICAL AND SEROLOGICAL STUDY OF PULMONARY ASPERGILLOSIS IN CENTRAL INDIA

2002 ◽  
Vol 20 (3) ◽  
pp. 141-144
Author(s):  
AM Kurhade ◽  
JM Deshmukh ◽  
RP Fule ◽  
C Chande ◽  
S Akulwar
Keyword(s):  
Central India ◽  
Serological Study ◽  
Pulmonary Aspergillosis

scholarly journals Cutaneous Leishmaniasis in Algeria; Highlight on the Focus of M’Sila

2021 ◽  
Vol 9 (5) ◽  
pp. 962
Author(s):  
Razika Beniklef ◽  
Karim Aoun ◽  
Karim Boudrissa ◽  
Meriem Ben Abid ◽  
Kamel Cherif ◽  
...  
Keyword(s):  
Risk Factors ◽  
Visceral Leishmaniasis ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Infantum ◽  
Psammomys Obesus ◽  
Phlebotomus Papatasi ◽  
Wild Rodents ◽  
High Incidence ◽  
Medical Importance ◽  
Underlying Risk

Algeria ranks second after Afghanistan for the incidence of cutaneous leishmaniasis (CL) worldwide. Here, we report a 34-years retrospective analysis of CL in Algeria and focused on the most affected region, the M’Sila province. All 66 cutaneous isolates corresponded to Leishmania (L.) major. Our study of the sandfly and rodent fauna further highlighted the high density of Phlebotomus papatasi and additional phlebotomine species of medical importance, not previously identified in M’Sila. Wild rodents belonging to nine species were trapped in M’Sila, and Psammomys obesus and Meriones shawi were found infected by L. major. In addition, Leishmania infantum was isolated from two visceral leishmaniasis cases, one dog and its proven vectors (P. perniciosus, P. longicuspis, and P. perfiliewi) inventoried during the survey. The high incidence of CL in the M’Sila province is likely a consequence of the increase in minimum temperatures recorded that constitutes suitable conditions for establishing a high endemicity and leads to an explosive rise in leishmaniases cases in this region. A thorough investigation of the underlying risk factors is urgently needed to detect new cases earlier. All these would improve the preparedness to fight the disease.

Leishmania infantum (syn. L. chagasi) parasites affect the release of soluble CD14 by infected macrophages

2021 ◽  
Author(s):  
Michelle Barreto Gomes Melo ◽  
Luana Celina Seraphim Cunha ◽  
Cárcia Santana Passos Barreto ◽  
Fabrícia Alvisi de Oliveira Mendonça ◽  
Micheli Luize Barbosa Santos ◽  
...  
Keyword(s):  
Leishmania Infantum ◽  
Soluble Cd14
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