The pH-stat Butyric Acid Feeding Strategy Coupled with Gas-Stripping for n-Butanol Production by Clostridium beijerinckii

Low-cost nitrogen sources, i.e., dried spent yeast (DSY), rice bran (RB), soybean meal (SM), urea and ammonium sulfate were used for batch butanol fermentation from sugarcane molasses by Clostridium beijerinckii TISTR 1461 under anaerobic conditions. Among these five low-cost nitrogen sources, DSY at 1.53 g/L (nitrogen content equal to that of 1 g/L of yeast extract) was found to be the most suitable. At an initial sugar level of 60 g/L, the maximum butanol concentration (PB), productivity (QB) and yield (YB/S) were 11.19 g/L, 0.23 g/L·h and 0.31 g/g, respectively. To improve the butanol production, the concentrations of initial sugar, DSY and calcium carbonate were varied using response surface methodology (RSM) based on Box–Behnken design. It was found that the optimal conditions for high butanol production were initial sugar, 50 g/L; DSY, 6 g/L and calcium carbonate, 6.6 g/L. Under these conditions, the highest experimental PB, QB and YB/S values were 11.38 g/L, 0.32 g/L·h and 0.40 g/g, respectively with 50% sugar consumption (SC). The PB with neither DSY nor CaCO3 was only 8.53 g/L. When an in situ gas stripping system was connected to the fermenter to remove butanol produced during the fermentation, the PB was increased to 15.33 g/L, whereas the YB/S (0.39 g/g) was not changed. However, the QB was decreased to 0.21 g/L·h with 75% SC.

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Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii: Part II—Fed-batch fermentation

Biomass and Bioenergy ◽

10.1016/j.biombioe.2007.07.005 ◽

2008 ◽

Vol 32 (2) ◽

pp. 176-183 ◽

Cited By ~ 76

Author(s):

Nasib Qureshi ◽

Badal C. Saha ◽

Michael A. Cotta

Keyword(s):

Wheat Straw ◽

Batch Fermentation ◽

Simultaneous Saccharification And Fermentation ◽

Clostridium Beijerinckii ◽

Butanol Production ◽

Fed Batch ◽

Fed Batch Fermentation ◽

Simultaneous Saccharification

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Fed-batch fermentation for n-butanol production from cassava bagasse hydrolysate in a fibrous bed bioreactor with continuous gas stripping

Bioresource Technology ◽

10.1016/j.biortech.2011.10.089 ◽

2012 ◽

Vol 104 ◽

pp. 380-387 ◽

Cited By ~ 163

Author(s):

Congcong Lu ◽

Jingbo Zhao ◽

Shang-Tian Yang ◽

Dong Wei

Keyword(s):

Batch Fermentation ◽

Butanol Production ◽

Gas Stripping ◽

Fed Batch ◽

Fibrous Bed Bioreactor ◽

Cassava Bagasse ◽

Fed Batch Fermentation

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Comparison of different Vetiver grass pretreatment techniques and their impact on immobilized butanol production by Clostridium beijerinckii TISTR 1461

Cellulose ◽

10.1007/s10570-021-04101-4 ◽

2021 ◽

Author(s):

Fuangfa Srisuk ◽

Piyawat Chinwatpaiboon ◽

Thanaphat Atjayutpokin ◽

Akarin Boonsombuti ◽

Ancharida Savarajara ◽

...

Keyword(s):

Clostridium Beijerinckii ◽

Vetiver Grass ◽

Butanol Production

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Production of butyric acid at constant pH by a solventogenic strain of Clostridium beijerinckii

Czech Journal of Food Sciences ◽

10.17221/95/2020-cjfs ◽

2020 ◽

Vol 38 (No. 3) ◽

pp. 185-191 ◽

Cited By ~ 2

Author(s):

Marek Drahokoupil ◽

Petra Patáková

Keyword(s):

Flow Cytometry ◽

Butyric Acid ◽

Population Viability ◽

Clostridium Beijerinckii ◽

Clostridium Tyrobutyricum ◽

Fluorescence Staining ◽

Low Concentration ◽

Ph Values ◽

Constant Ph ◽

Acetone Butanol Ethanol

A solventogenic strain of Clostridium beijerinckii, NRRL B-598, was cultured for the production of butyric acid as the main fermentation product. However, unlike typical acetone-butanol-ethanol (ABE) fermentations, where pH is not regulated, in this study the pH was kept constant during fermentation. From the five pH values tested, 6.0, 6.5, 7.0, 7.5 and 8.0, pH 6.5 and 7.0 resulted in the highest concentrations of butyric acid, at 9.69 ± 0.09 g L–1 and 11.5 ± 0.39 g L–1, respectively. However, a low concentration of solvents, 1.8 ± 0.22 g L–1, was only reached at pH 7.0. These results are comparable with those from typical butyric acid producers, i.e. Clostridium butyricum and Clostridium tyrobutyricum strains. At pH 7.0, we succeeded in suppressing sporulation and prolonging the population viability, which was confirmed by flow cytometry combined with double fluorescence staining.

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Developing a coculture for enhanced butanol production by Clostridium beijerinckii and Saccharomyces cerevisiae

Bioresource Technology Reports ◽

10.1016/j.biteb.2019.03.006 ◽

2019 ◽

Vol 6 ◽

pp. 223-228 ◽

Cited By ~ 10

Author(s):

Jiwen Wu ◽

Lili Dong ◽

Chunshuang Zhou ◽

Bingfeng Liu ◽

Liping Feng ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Clostridium Beijerinckii ◽

Butanol Production

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Milling byproducts are an economically viable substrate for butanol production using clostridial ABE fermentation

Applied Microbiology and Biotechnology ◽

10.1007/s00253-020-10882-8 ◽

2020 ◽

Vol 104 (20) ◽

pp. 8679-8689

Author(s):

Nils Thieme ◽

Johanna C. Panitz ◽

Claudia Held ◽

Birgit Lewandowski ◽

Wolfgang H. Schwarz ◽

...

Keyword(s):

Clostridium Beijerinckii ◽

Abe Fermentation ◽

Liquid Fuels ◽

Butanol Production ◽

Enzymatic Pretreatment ◽

Profitability Analysis ◽

Key Points ◽

Wide Range ◽

Wheat Middlings ◽

Acetone Butanol Ethanol

Abstract Butanol is a platform chemical that is utilized in a wide range of industrial products and is considered a suitable replacement or additive to liquid fuels. So far, it is mainly produced through petrochemical routes. Alternative production routes, for example through biorefinery, are under investigation but are currently not at a market competitive level. Possible alternatives, such as acetone-butanol-ethanol (ABE) fermentation by solventogenic clostridia are not market-ready to this day either, because of their low butanol titer and the high costs of feedstocks. Here, we analyzed wheat middlings and wheat red dog, two wheat milling byproducts available in large quantities, as substrates for clostridial ABE fermentation. We could identify ten strains that exhibited good butanol yields on wheat red dog. Two of the best ABE producing strains, Clostridium beijerinckii NCIMB 8052 and Clostridium diolis DSM 15410, were used to optimize a laboratory-scale fermentation process. In addition, enzymatic pretreatment of both milling byproducts significantly enhanced ABE production rates of the strains C. beijerinckii NCIMB 8052 and C. diolis DSM 15410. Finally, a profitability analysis was performed for small- to mid-scale ABE fermentation plants that utilize enzymatically pretreated wheat red dog as substrate. The estimations show that such a plant could be commercially successful. Key points • Wheat milling byproducts are suitable substrates for clostridial ABE fermentation. • Enzymatic pretreatment of wheat red dog and middlings increases ABE yield. • ABE fermentation plants using wheat red dog as substrate are economically viable.

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