Antifungal Efficacy of Chitosan-Stabilized Biogenic Silver Nanoparticles against Pathogenic Candida spp. Isolated from Human

2020 ◽  
Vol 10 (4) ◽  
pp. 974-982
Author(s):  
Smitha Vijayan ◽  
K. Divya ◽  
Sherin Varghese ◽  
M. S. Jisha
Keyword(s):  
Silver Nanoparticles ◽  
Candida Spp ◽  
Biogenic Silver Nanoparticles ◽  
Antifungal Efficacy

scholarly journals Biodirected Synthesis of Silver Nanoparticles Using Aqueous Honey Solutions and Evaluation of Their Antifungal Activity against Pathogenic Candida Spp.

2021 ◽  
Vol 22 (14) ◽  
pp. 7715
Author(s):  
Grzegorz Czernel ◽  
Dominika Bloch ◽  
Arkadiusz Matwijczuk ◽  
Jolanta Cieśla ◽  
Monika Kędzierska-Matysek ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Fungal Growth ◽  
Disc Diffusion ◽  
Candida Spp ◽  
Antifungal Effect ◽  
Synthesis Of Nanoparticles ◽  
Honey Solution ◽  
Diffusion Assay

Silver nanoparticles (AgNPs) were synthesized using aqueous honey solutions with a concentration of 2%, 10%, and 20%—AgNPs-H2, AgNPs-H10, and AgNPs-H20. The reaction was conducted at 35 °C and 70 °C. Additionally, nanoparticles obtained with the citrate method (AgNPs-C), while amphotericin B (AmB) and fluconazole were used as controls. The presence and physicochemical properties of AgNPs was affirmed by analyzing the sample with ultraviolet–visible (UV–Vis) and fluorescence spectroscopy, scanning electron microscopy (SEM), and dynamic light scattering (DLS). The 20% honey solution caused an inhibition of the synthesis of nanoparticles at 35 °C. The antifungal activity of the AgNPs was evaluated using opportunistic human fungal pathogens Candida albicans and Candida parapsilosis. The antifungal effect was determined by the minimum inhibitory concentration (MIC) and disc diffusion assay. The highest activity in the MIC tests was observed in the AgNPs-H2 variant. AgNPs-H10 and AgNPs-H20 showed no activity or even stimulated fungal growth. The results of the Kirby–Bauer disc diffusion susceptibility test for C. parapsilosis strains indicated stronger antifungal activity of AgNPs-H than fluconazole. The study demonstrated that the antifungal activity of AgNPs is closely related to the concentration of honey used for the synthesis thereof.

scholarly journals Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles

2016 ◽  
Vol 205 (6) ◽  
pp. 603-613 ◽  
Author(s):  
M. Składanowski ◽  
P. Golinska ◽  
K. Rudnicka ◽  
H. Dahm ◽  
M. Rai
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Biogenic Silver Nanoparticles

Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles

2021 ◽  
Vol 297 ◽  
pp. 113434
Author(s):  
Federico N. Spagnoletti ◽  
Florencia Kronberg ◽  
Cecilia Spedalieri ◽  
Eliana Munarriz ◽  
Romina Giacometti
Keyword(s):  
Silver Nanoparticles ◽  
Protein Corona ◽  
Biogenic Silver Nanoparticles

Optimization and Characterization of Biogenic Silver Nanoparticles Synthesized by Leaves Extract of Alphonsea Madraspatana

2021 ◽  
Vol 17 ◽  
Author(s):  
Amita Sahu ◽  
Sudhanshu Shekhar Swain ◽  
Goutam Ghosh ◽  
Deepak Pradhan ◽  
Dipak Kumar Sahu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Volume Ratio ◽  
Xrd Analysis ◽  
Biofilm Inhibition ◽  
Nano Silver ◽  
E Coli ◽  
Biogenic Silver Nanoparticles ◽  
Icp Ms ◽  
Anti Oxidant

Background: Literature evidences as well as traditional uses of genus Alphonsea reveal significant antimicrobial and anti-oxidant activity, which encourages to consider A. madraspatana to have potent antimicrobials, there by offering potential adjuncts to synthesize improved antimicrobial Silver nanoparticles (AgNPs). The objective of the present exposition is to optimize reaction parameters to synthesize antimicrobial Biogenic Silver nanoparticles (BAgNPs) from extract of A. madraspatana leaves (AML) and to evaluate the effect against bacteria. Methods: BAgNPs was synthesized by optimized reaction. The Synthesized nanoparticles were characterized by UV, IR, ICP-MS and XRD analysis. The antibacterial potency of optimized BAgNPs was evaluated against E. coli by comparing with positive controls. Results: Results of optimization process indicate nanoscale BAgNPs were produced at operating temp. of 45°C for 120 min at pH 8 with 1:5 volume ratio of AgNO3 and extract. Optimized BAgNPs exhibits relatively higher antimicrobial activity (31±1mm) compared to Ciprofloxacin (27±1mm) and marketed nano silver (28± 2 mm). The developed BAgNPs shows comparable biofilm inhibition (86.50%) as compared to marketed nano silver (88.10%) and Ciprofloxacin (83.10%). Conclusion: Experimental evidence suggests methanolic extract of AML under predefined conditions successfully generate nano-template of silver with better antibacterial response against E. coli.

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