Manganese and cobalt complexes have been designed and prepared with a tetradentate ligand i.e.
isopthalic acid-bis(thiosemicarbozone) (IPBT), which bind to metal ions via donor atoms present in
ligand. Different spectroscopic techniques viz. nuclear magnetic resonance, infra red, mass, electronic
spin resonance and analytical studies have been used to determine the chemical composition of
synthesized IPBT and its Mn(II) and Co(II) complexes. The spectroscopic data exposed that IPBT
behaves in a tetradentate (N2S2) mode by having ability to bind with metal ions through N2S2 atoms.
An octahedral structure for manganese and cobalt complexes has been suggested on the basis of
spectroscopic as well as analytical studies. The ligand (IPBT) and its metal(II) complexes have been
screened to determine their antipathogenic activity against some selective microorganisms S. aureus,
P. aeruginosa, E. coli, A. niger, M. phasolina and P. glomerata. In this experimental work, well diffusion
and poisoned food techniques have been introduced for screening purpose and as standard drugs
neomycin and chlorothalonil have been used. Data for antipathogenic screening exposed that metal
complexes exerted higher activity towards all examined microbes (bacteria and fungi) even than ligand.
The production of cinnamic acid secondary metabolites through in vitro culture of callus Camellia sinensis L with the elicitor of cobalt metal ions