scholarly journals Bonding Ability of Isopthalic Acid-bis(thiosemicarbozone) to Manganese and Cobalt Metal Ions: Preparation, Spectral Investigation, Computational and in vitro Antipathogenic Screening

2022 ◽  
Vol 34 (2) ◽  
pp. 311-318
Author(s):  
S. Gautam ◽  
P. Pipil ◽  
A. Kumar ◽  
Alka ◽  
J. Singh ◽  
...  
Keyword(s):  
Metal Ions ◽  
Cobalt Complexes ◽  
Cobalt Metal ◽  
Spectroscopic Techniques ◽  
E Coli ◽  
Electronic Spin ◽  
Analytical Studies ◽  
Spin Resonance ◽  
Bacteria And Fungi

Manganese and cobalt complexes have been designed and prepared with a tetradentate ligand i.e. isopthalic acid-bis(thiosemicarbozone) (IPBT), which bind to metal ions via donor atoms present in ligand. Different spectroscopic techniques viz. nuclear magnetic resonance, infra red, mass, electronic spin resonance and analytical studies have been used to determine the chemical composition of synthesized IPBT and its Mn(II) and Co(II) complexes. The spectroscopic data exposed that IPBT behaves in a tetradentate (N2S2) mode by having ability to bind with metal ions through N2S2 atoms. An octahedral structure for manganese and cobalt complexes has been suggested on the basis of spectroscopic as well as analytical studies. The ligand (IPBT) and its metal(II) complexes have been screened to determine their antipathogenic activity against some selective microorganisms S. aureus, P. aeruginosa, E. coli, A. niger, M. phasolina and P. glomerata. In this experimental work, well diffusion and poisoned food techniques have been introduced for screening purpose and as standard drugs neomycin and chlorothalonil have been used. Data for antipathogenic screening exposed that metal complexes exerted higher activity towards all examined microbes (bacteria and fungi) even than ligand.

scholarly journals Biosurfactants Induce Antimicrobial Peptide Production through the Activation of TmSpatzles in Tenebrio molitor

2020 ◽  
Vol 21 (17) ◽  
pp. 6090
Author(s):  
Tariku Tesfaye Edosa ◽  
Yong Hun Jo ◽  
Maryam Keshavarz ◽  
In Seon Kim ◽  
Yeon Soo Han
Keyword(s):  
Antimicrobial Peptide ◽  
Immune Activation ◽  
Tenebrio Molitor ◽  
Antimicrobial Activities ◽  
Iturin A ◽  
E Coli ◽  
Fat Bodies ◽  
Escherichia Coli Bacteria ◽  
Bacteria And Fungi

Biosurfactant immunomodulatory activities in mammals, nematodes, and plants have been investigated. However, the immune activation property of biosurfactants in insects has not been reported. Therefore, here, we studied the defense response triggered by lipopeptides (fengycin and iturin A), glycolipids (rhamnolipid), and cyclic polypeptides (bacitracin) in the coleopteran insect, mealworm Tenebrio molitor. The in vitro antimicrobial activities against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and fungi (Candida albicans) were assessed by mixing these pathogens with the hemolymph of biosurfactant-immune-activated larvae. E. coli growth was remarkably inhibited by this hemolymph. The antimicrobial peptide (AMP) induction results also revealed that all biosurfactants tested induced several AMPs, exclusively in hemocytes. The survivability analysis of T. molitor larvae challenged by E. coli (106 CFU/µL) at 24 h post biosurfactant-immune activation showed that fengycin, iturin A, and rhamnopid significantly increased survivability against E. coli. Biosurfactant-induced TmSpatzles activation was also monitored, and the results showed that TmSpz3 and TmSpz-like were upregulated in the hemocytes of iturin A-injected larvae, while TmSpz4 and TmSpz6 were upregulated in the fat bodies of the fengycin-, iturin A-, and rhamnolipid-injected larvae. Overall, these results suggest that lipopeptide and glycolipid biosurfactants induce the expression of AMPs in T. molitor via the activation of spätzle genes, thereby increasing the survivability of T. molitor against E. coli.

scholarly journals Phytochemical characterization, anti-cancer and antimicrobial activity of isolated fractions of Alysicarpus vaginalis

2017 ◽  
Vol 12 (1) ◽  
pp. 77 ◽  
Author(s):  
Ganesh Tapadiya ◽  
Mayura A. Kale ◽  
Shweta Saboo
Keyword(s):  
Antimicrobial Activity ◽  
Cell Lines ◽  
Methanolic Extract ◽  
Hep G2 ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Anti Cancer ◽  
Proliferative Assay ◽  
Bacteria And Fungi

<p class="Abstract">The methanolic extract of <em>Alysicarpus </em>vaginalis was selected for fractionation due to its known reported biological activity. The four fractions were separated and subjected for<em> in vitro</em> antimitotic and anti-proliferative assays along with anti-cancer activity on two human cancers cell lines (SK-MEL-2 and Hep-G2). The antimicrobial potential of fractions had been evaluated against bacteria and fungi. From all fractions, acetone and n-butanol fractions were effective against the cell lines. They show strong inhibitory action with mitotic index 6.2 and 8.4 mg/mL and IC<sub>50 </sub>values of anti-proliferative assay in between 19.7 to 14.2 mg/mL respectively, which was found to be comparable to the standard methothrexate 5.9 mg/mL and 13.2 mg/mL respectively. In antimicrobial activity, the zone of inhibition had been observed in the range of 12-27 mm and MIC value was found in the range of 0.2-0.1 mg/mL. The acetone fraction was found to be most active against fungi, and<em> E. coli</em> whereas chloroform and n-butanol fractions were more effective against <em>S. aureus</em> and <em>B. </em>subtilis. The phytochemical characterization by HPLC analysis indicated the presence of important polyphenolic and steroidal compounds.</p>

scholarly journals In vitro Effects of Muscodor albus and Three Volatile Components on Growth of Selected Postharvest Microorganisms

HortScience ◽  
2005 ◽  
Vol 40 (7) ◽  
pp. 2109-2114 ◽  
Author(s):  
Ali A. Ramin ◽  
P. Gordon Braun ◽  
Robert K. Prange ◽  
John M. DeLong
Keyword(s):  
Erwinia Carotovora ◽  
Isobutyric Acid ◽  
Penicillium Expansum ◽  
Volatile Components ◽  
E Coli ◽  
In Vitro Effects ◽  
Bacteria And Fungi ◽  
Air Circulation ◽  
Muscodor Albus

Biofumigation by volatiles of Muscodor albus Worapong, Strobel & W.M. Hess, an endophytic fungus, was investigated for the biological control of three postharvest fungi, Botrytis cinerea Pers., Penicillium expansum Link, and Sclerotinia sclerotiorum (Lib) de Bary, and three bacteria, Erwinia carotovora pv. carotovora (Jones) Bergey et al., Pseudomonas fluorescens Migula (isolate A7B), and Escherichia coli (strain K12). Bacteria and fungi on artificial media in petri dishes were exposed to volatiles produced by M. albus mycelium growing on rye seeds in sealed glass 4-L jars with or without air circulation for up to 48 hours. The amount of dry M. albus–rye seed culture varied from 0.25 to 1.25 g·L–1 of jar volume. Fan circulation of volatiles in jars increased efficacy and 0.25 g·L–1 with fan circulation was sufficient to kill or suppress all fungi and bacteria after 24 and 48 hours, respectively. Two major volatiles of M. albus, isobutyric acid (IBA) and 2-methyl-1-butanol (MB), and one minor one, ethyl butyrate (EB), varied in their control of the same postharvest fungi and bacteria. Among the three fungi, IBA killed or suppressed S. sclerotiorum, B. cinerea, and P. expansum at 40, 25, and 45 μL·L –1, respectively. MB killed or suppressed S. sclerotiorum, B. cinerea, and P. expansum at 75, 100, and 100 μL·L –1, respectively. EB was only able to kill S. sclerotiorum at 100 μL·L –1. Among the three bacteria, IBA killed or suppressed E. coli (K12), E. carotovora pv. carotovora, and P. fluorescens at 5, 12.5, and 12.5 μL·L–1, respectively. MB killed or suppressed E. coli (K12), E. carotovora pv. carotovora, and P. fluorescens at 100, 75, and 100 μL·L–1, respectively. EB did not control growth of the three bacteria. This study demonstrates the need for air circulation in M. albus, MB, and IBA treatments to optimize the efficacy of these potential postharvest agents of disease control.

scholarly journals Synthesis, Structural Characterization, Catalytic, Biological and α-Glucosidase Inhibitory Studies of Metal Complexes with Flavone Derivatives

2021 ◽  
Vol 36 (1) ◽  
pp. 265-276
Author(s):  
R.R. Krishna Jyothi ◽  
Sharow Geeth Vincent ◽  
J. Joseph
Keyword(s):  
Metal Complexes ◽  
Heterocyclic Compounds ◽  
Selective Inhibition ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
Metal Chelates ◽  
Composition Analysis ◽  
Spectroscopic Techniques ◽  
E Coli

Heterocyclic compounds, in particular oxygen-containing heterocyclic compounds, are of special interest to medicinal chemists because of their unusual biological properties. In the present study, the highly conjugated nitrogen heterocyclic scaffold comprised of flavone derivative with metal acetates to form metal chelates of the type [MIIL(OAc)2], flavone analogues (L); M=Co2+, Zn2+, Cu2+ and Ni2+. The above title compounds were characterized using composition analysis o fCHN and spectroscopic techniques. Based on spectroscopic and analytical measurements confirmed that square planar arrangements for the Co2+, Zn2+, Cu2+ and Ni2+ complexes. Antimicrobial efficacy of prepared complexes were assessed against A.flavus, A.niger, B.subtilis, E. coli, C. albicans and S.aureus. The anti-mycobacterial (H37Rv) efficacy of flavone analogues and its complexes were screened using MABA approach and compared with standard. The acetylcholinesterase (AChE) inhibitory effect of the ligand was examined to find out the therapeutic efficiency of compound in the treatment of neurodegenerative disorders. The synthesized ligand exhibited selective inhibition (AChE & BuChe) values (IC50:0.20(flavone analogue), 2.41 (Rivastigmine) and 3.01 µM (Galantamine), respectively. Further, the in vitro ant-inflammatory efficiency of metal chelates were performed with the help of egg albumin method. The α-glucosidase inhibition activity was also carried out for the prepared metal complexes.

Synthesis and Structural Elucidation of Aminoacetylenic Derivatives of 7-Methoxy- 2-Naphthole as Antimicrobial Agents

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
Abu- Safieh Rana ◽  
Muhi- Eldeen Zuhair ◽  
Alsarahni Aseel ◽  
Al-Kaissi Elham
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Naphthalene Derivatives ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Bacteria And Fungi ◽  
Aminoacetylenic Derivatives ◽  
Derivatives Of ◽  
Mic Value

A new series of 7-methoxy-2-[4-(t-amino-1-yl)oxy]-naphthalene derivatives; 7-methoxy-2-{[4-(2-methylpiperidine)but-2-yn-1-yl]oxy}-naphthalene (RZ2), 7-methoxy-2-{[4-(2,6-dimethylpiperidine)but-2-yn-1-yl]oxy}-naphthalene (RZ3), 7-methoxy-2{[4-(piperidine)but-2-yn-1-yl]oxy}-naphthalene (RZ4), 7-methoxy-2-{[4-(pyrrolidine)but-2-yn-1-yl]oxy}-naphthalene (RZ5), 7-methoxy-2-{[4-(N-methylpiperazine)but-2-yn-1-yl]oxy}-naphthalene (RZ6), 7-methoxy -2-{[4-(hexamethyleneimine)but-2-yn-1-yl]oxy}-naphthalene (RZ7) were synthesized and screened in vitro as potential antimicrobial agents. Antimicrobial activity were evaluated by measuring the minimum inhibitory and bactericidal/fungicidal concentration (MIC, MBC and MFC). RZ2, RZ5, RZ6 and RZ7 showed the highest antimicrobial activity against S. aureus with MIC value 62.5 µg/ml, compounds RZ2, RZ4, RZ5, and RZ7 have the highest antimicrobial activity against B. subtilis with MIC vale 62.5 µg/ml, RZ3, RZ6 have the same antimicrobial activity with MIC value 125µg/ml, compounds. RZ4, RZ5, RZ6 and RZ7 have the highest antimicrobial activity against E. coli with MIC value 125 µg/ml, all compounds have the same MIC value against P. aeruginosa (125 µg/ml). RZ2, RZ4, RZ5, RZ6, RZ7 showed the highest antifungal activity with MIC of 62.5 µg/ml. In conclusion, the synthesized compounds showed good antimicrobial activity and promising potency against gram positive bacteria, gram negative bacteria and fungi.

scholarly journals Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Babe Guyasa ◽  
Yadessa Melaku ◽  
Milkyas Endale
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Fungal Infections ◽  
2D Nmr ◽  
Stem Bark ◽  
Spectroscopic Techniques ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
E Coli

Embelia schemperi Vatke is one of the medicinal plants used traditionally for treatment of intestinal tape worm, dysmenorrheal, bacterial, and fungal infections. Phytochemical screening test of the dichloromethane/methanol (1 : 1) and methanol extracts revealed the presence of phenols, alkaloids, tannins, and flavonoids whereas terpenoids, glycoside, and phytosterols were absent. Silica gel column chromatographic separation of the methanol extract afforded 3,5,7,3′,4′-pentahydroxyflavan, named epicatechin (1), along with a close flavan derivative (2). Structures of the compounds were elucidated by spectroscopic techniques (1D and 2D NMR, FTIR, and UV-Vis). The crude extracts and isolated compounds were screened for in vitro antibacterial activity against strains of Klebsiella pneumoniae, Escherichia coli, Cryptococcus neoformans, Shigella dysentriae, and Staphylococcus aureus. Epicatechin (1) exhibited comparable antibacterial activity against S. aureus and E. coli (15 and 12 mm zone of inhibition, resp.) to that of the control antibiotic gentamicin, with zone of inhibition of 15 and 12 mm, respectively, at a concentration of 20 µg/mL.

scholarly journals Synthesis, characterization, and biological properties of oxidovanadium(IV) complexes of acetylsalicylhydroxamic acid (N-acetyloxy-2-hydroxybenzamide) as potential antimicrobials

2020 ◽  
Vol 44 (7-8) ◽  
pp. 460-470
Author(s):  
Bhanu Priya ◽  
Abhishek Kumar ◽  
Neeraj Sharma
Keyword(s):  
Electron Spin Resonance ◽  
Electron Spin ◽  
Magnetic Moment ◽  
Biological Properties ◽  
Molar Ratio ◽  
Pyramidal Geometry ◽  
Concentration Method ◽  
Spin Resonance ◽  
Bacteria And Fungi

New oxidovanadium(IV) complexes of composition [VO(AcSHA)2] 1 and [VO(acac)(AcSHA)] 2 are synthesized by reactions of VOSO4.5H2O and [VO(acac)2] with acetylsalicylhydroxamic acid AcSH2A (C6H4(OH)(CONHOCOCH3)) in a 1:2 molar ratio in absolute ethanol. The compounds are characterized by the Fourier-transform infrared spectroscopy, ultraviolet–visible spectroscopy, electron spin resonance, and mass spectrometry along with elemental analyses, molar conductivity, and magnetic moment measurements. The infrared spectra of the complexes suggest bonding through carbonyl and phenolic oxygen atoms (O,O coordination). The magnetic moment, electron spin resonance, and mass spectra of the complexes indicate that both exist as monomers, and a distorted square pyramidal geometry around vanadium is proposed. The thermal behavior of the complexes is studied by thermogravimetry and differential thermal analysis techniques under an N2 atmosphere, yielding VO2 as the decomposition product. The in vitro antimicrobial assays against pathogenic Gram-positive bacteria, Gram-negative bacteria, and fungi (minimum inhibitory concentration method) show the appreciable antimicrobial potential relative to the respective standard drugs, tetracycline hydrochloride, and fluconazole.

scholarly journals Antioxidant Flavonoids from Asteriscus Maritimus

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200
Author(s):  
Habiba Daroui-Mokaddem ◽  
Ahmed Kabouche ◽  
Naima Boutaghane ◽  
Claude-Alain Calliste ◽  
Jean-Luc Duroux ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Flavonol Glycoside ◽  
Spectroscopic Techniques ◽  
Dpph Radical ◽  
Antioxidative Effect ◽  
H Nmr ◽  
Chemical Structures ◽  
Electronic Spin ◽  
Spin Resonance ◽  
C Nmr

One new flavonol glycoside, patuletin-7- O-[6- O-caffeoyl-2- O-[(S)-3-hydroxyisobutanoyl]glucopyranoside] (astermaritimoside) (1) and four known flavonoids, patuletin-7- O-β-[-6- O-[( S)-3-hydroxyisobutanoyl]glucopyranoside] (2), patuletin-7- O-[6- O-caffeoylglucopyranoside] (3), patuletin-7- O-β-D-glucopyranoside (4) and quercetin-3- O-β-rutinoside (5) have been isolated from Asteriscus maritimus (L.) Less. Chemical structures were elucidated by mass spectrometric, and 1H NMR, 13C NMR, COSY, HMQC and HMBC spectroscopic techniques. The antioxidative effect was evaluated using an electronic spin resonance (ESR) method in order to visualize the inhibition of the DPPH radical. The n-butanol fraction was found to possess a remarkable antioxidant activity that was correlated with the total amount of phenolics. The isolated compounds exhibited good antioxidant activity.

scholarly journals Novel Zinc(II) Complexes of Heterocyclic Ligands as Antimicrobial Agents: Synthesis, Characterisation, and Antimicrobial Studies

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Ramesh S. Yamgar ◽  
Y. Nivid ◽  
Satish Nalawade ◽  
Mustapha Mandewale ◽  
R. G. Atram ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Schiff Base ◽  
Metal Complexes ◽  
Antimicrobial Agents ◽  
Spectroscopic Techniques ◽  
Schiff Base Ligands ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Antimicrobial Studies

The synthesis and antimicrobial activity of novel Zn(II) metal complexes derived from three novel heterocyclic Schiff base ligands 8-[(Z)-{[3-(N-methylamino)propyl]imino}methyl]-7-hydroxy-4-methyl-2H-chromen-2-one, 2-[(E)-{[4-(1H-1,2,4-triazol-1-ylmethyl)phenyl]imino}methyl]phenol, and (4S)-4-{4-[(E)-(2-hydroxybenzylidene)amino]benzyl}-1,3-oxazolidin-2-one have been described. These Schiff base ligands and metal complexes are characterised by spectroscopic techniques. According to these data, we propose an octahedral geometry to all the metal complexes. Antimicrobial activity of the Schiff base ligand and its metal complexes was studied against Gram negative bacteria:E. coliandPseudomonas fluorescens, Gram positive bacteria:Staphylococcus aureus,and also against fungi, that is,C. albicansandA. niger. Some of the metal complexes show significant antifungal activity (MIC < 0.2 μg/mL). The “in vitro” data has identified [Zn(NMAPIMHMC)2]·2H2O, [Zn(TMPIMP)2]·2H2O, and [Zn(HBABO)2]·2H2O as potential therapeutic antifungal agents againstC. albicansandA. niger.

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