Angiolipoleiomyoma or HMB-45 Negative Angiomyolipoma of Uterus: Need for Uniformity in Terminology

2021 ◽  
Author(s):  
Neha Garg ◽  
Nadeem Tanveer
Keyword(s):  
Hmb 45

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

scholarly journals A Case of Primary Subglottic Malignant Melanoma with a Successful Surgical Treatment

2014 ◽  
Vol 2014 ◽  
pp. 1-3
Author(s):  
Shahzad Ahmad ◽  
Mahmoud Abdelghany ◽  
Curtis Goldblatt ◽  
Owen Stark ◽  
Nicholas Masciotra
Keyword(s):  
Surgical Treatment ◽  
Malignant Melanoma ◽  
Excisional Biopsy ◽  
Human Melanoma ◽  
Primary Malignant Melanoma ◽  
Shortness Of Breath ◽  
S 100 ◽  
Hmb 45

Primary subglottic malignant melanoma is a very rare and underdiagnosed neoplasm. We are reporting a case of primary malignant melanoma of subglottic mucosa in a 78-year-old woman who presented to our hospital with shortness of breath and hoarseness of voice. Laryngoscopy and excisional biopsy along with immunoreactivity to S-100 and human melanoma black-45 (HMB-45) confirmed the diagnosis. The patient was treated with laryngectomy followed by radiotherapy. Five years following surgical treatment, she continues to be asymptomatic. To our knowledge, there is only one reported case of primary malignant melanoma of subglottic mucosa in the medical literatures.

Differential Expression of Melanocytic Markers in Myoid, Lipomatous, and Vascular Components of Renal Angiomyolipomas

2007 ◽  
Vol 131 (1) ◽  
pp. 122-125 ◽  
Author(s):  
Andres A. Roma ◽  
Cristina Magi-Galluzzi ◽  
Ming Zhou
Keyword(s):  
Smooth Muscle ◽  
Blood Vessel ◽  
Blood Vessels ◽  
Differential Expression ◽  
Tumor Cells ◽  
Tissue Microarray ◽  
Expression Patterns ◽  
Renal Angiomyolipoma ◽  
Melanocytic Lesions ◽  
Hmb 45

Abstract Context.—Renal angiomyolipoma is a tumor composed of varying amounts of fat, smooth muscle, and blood vessels. Characteristically, tumor cells express melanocytic markers such as HMB-45 and Melan-A. Recently, several other markers have been described as having excellent diagnostic sensitivity in cutaneous melanocytic lesions. Objectives.—To compare the sensitivities of 5 melanocytic markers in renal angiomyolipoma and to study the expression patterns of these markers in the 3 different components of angiomyolipoma. Design.—A tissue microarray of 20 renal angiomyolipomas was constructed. For each case, 3 cores containing fat, blood vessels, and smooth muscle were taken. The tissue microarray was then stained for HMB-45, Melan-A, tyrosinase, NK1-C3, and CD117. Results.—HMB-45 was positive in 95%, Melan-A in 85%, NK1-C3 in 70%, tyrosinase in 50%, and CD117 in 40% of the cases. All (20/20) were positive for HMB-45 and Melan-A combined. These 5 markers had different sensitivities in the 3 components. HMB-45 was positive in 90%, 85%, and 80% of fat, smooth muscle, and blood vessel components, respectively; Melan-A in 70%, 60%, and 40%; NK1-C3 in 55%, 55%, and 45%; tyrosinase in 30%, 40%, and 10%; and CD117 in 20%, 40%, and 10%, respectively, of these 3 components. Conclusions.—HMB-45 and Melan-A combined were positive in 100% of the renal angiomyolipomas. We recommend the use of these 2 markers in the workup of this entity, including those with predominantly 1 component. Other melanocytic markers are of limited use. A tissue block comprising predominantly fat or smooth muscle components should be used when performing melanocytic marker immunostain.

Clear Cell Ductal Adenocarcinoma of Pancreas: A Case Report and Review of the Literature

2004 ◽  
Vol 128 (6) ◽  
pp. 693-696 ◽  
Author(s):  
Sourav Ray ◽  
Zhengbin Lu ◽  
Swaminathan Rajendiran
Keyword(s):  
Clear Cell ◽  
Clear Cell Carcinoma ◽  
Ductal Adenocarcinoma ◽  
Molecular Evidence ◽  
Cytokeratin 20 ◽  
Ras Oncogene ◽  
Cytokeratin 7 ◽  
Review Of The Literature ◽  
Hyaline Globules ◽  
Hmb 45

Abstract We present a unique carcinoma of the pancreas with predominantly clear cell morphology (>95% clear cells). Mucicarmine stain revealed abundant intraluminal and intracytoplasmic mucin. Immunohistochemically, the cells were positive for the epithelial markers cytokeratin 7 and CAM 5.2, and were focally positive for cytokeratin 20. These cells also expressed monoclonal carcinoembryonic antigen. Stains for the neuroendocrine markers synaptophysin and chromogranin were negative, as were stains for vimentin, p53, HMB-45, and CD10. An additional outstanding feature was the presence of dense intraluminal and intracytoplasmic hyaline globules, which were immunohistochemically positive for α1-antitrypsin. Sequencing of the K-ras oncogene revealed a point mutation in codon 12, providing molecular evidence of ductal origin. In the proper morphologic context supported by immunohistochemistry, clear cell carcinoma can be regarded as a rare variant of ductal adenocarcinoma.

Atypical Angiomyolipoma of Kidney in a Patient With Tuberous Sclerosis: A Case Report With p53 Gene Mutation Analysis

2005 ◽  
Vol 129 (5) ◽  
pp. 676-679 ◽  
Author(s):  
Linglei Ma ◽  
Diane Kowalski ◽  
Khurram Javed ◽  
Pei Hui
Keyword(s):  
Tuberous Sclerosis ◽  
P53 Gene ◽  
Epithelioid Cell ◽  
P53 Mutation ◽  
P53 Gene Mutation ◽  
Perivascular Epithelioid Cell ◽  
The Family ◽  
P53 Immunoreactivity ◽  
Gene Mutation Analysis ◽  
Hmb 45

Abstract Angiomyolipoma (AML) is the most common benign mesenchymal tumor of the kidney. It belongs to the family of perivascular epithelioid cell tumors and is typically composed of blood vessels, adipose tissue, and smooth muscle– like cells, which are characteristically positive for HMB-45. Results of recent studies suggest that p53 mutation may play an important role in AML progression. Here, we describe a locally destructive renal AML in a patient with tuberous sclerosis. The tumor consisted of mostly epithelioid cells with marked nuclear pleomorphism and frequent mitoses and was positive for HMB-45. The diagnosis of atypical epithelioid AML was made. Codon alteration in the p53 gene was not detected, despite focal p53 immunoreactivity and single nucleotide polymorphism at exon 6. Our finding indicates no definite link between p53 abnormalities and the atypical appearance of AML. To the best of our knowledge, this is the second renal AML case investigated for p53 mutations.

Pagetoid Bowen Disease

2000 ◽  
Vol 124 (3) ◽  
pp. 427-430 ◽  
Author(s):  
John D. Williamson ◽  
Maria I. Colome ◽  
Aysegul Sahin ◽  
Alberto G. Ayala ◽  
L. Jeffrey Medeiros
Keyword(s):  
Differential Diagnosis ◽  
Carcinoma In Situ ◽  
Clinical Evidence ◽  
Malignant Neoplasm ◽  
The Other ◽  
Paget Disease ◽  
Melanoma In Situ ◽  
Bowen Disease ◽  
Hmb 45

Abstract Bowen disease is a variant of squamous cell carcinoma in situ. In some cases a pagetoid growth pattern can be observed with cytologically atypical clear cells arranged singly and in nests. The differential diagnosis of pagetoid Bowen disease includes primarily Paget disease and malignant melanoma in situ, as well as other less common entities. Two cases of pagetoid Bowen disease are described, one in a 65-year-old man with a thigh lesion and the other in a 25-year-old man with a lesion in the penile/scrotal region. Neither patient had clinical evidence of an internal malignant neoplasm. In both cases, the neoplastic cells were positive for cytokeratin (CK) 7 and CK 19 and were negative for CK 18, CK 20, carcinoembryonic antigen, GCDFP-15, c-erbB2, S100, and HMB-45. In aggregate, these findings support the diagnosis of pagetoid Bowen disease. Previously, others have shown that CK 7 is an almost invariable marker of Paget disease. Thus, we report these two cases to illustrate that CK 7 can be expressed by pagetoid Bowen disease and should not be a cause of confusion in the differential diagnosis.

Tyrosinase Expression in Malignant Melanoma, Desmoplastic Melanoma, and Peripheral Nerve Tumors

2002 ◽  
Vol 126 (7) ◽  
pp. 816-822 ◽  
Author(s):  
Jenny L. Boyle ◽  
Helen M. Haupt ◽  
Jere B. Stern ◽  
Hinke A. B. Multhaupt
Keyword(s):  
Peripheral Nerve ◽  
S100 Protein ◽  
Dermatofibrosarcoma Protuberans ◽  
Antigen Retrieval ◽  
Nerve Sheath Tumors ◽  
Peripheral Nerve Sheath Tumors ◽  
Desmoplastic Melanoma ◽  
Nerve Sheath ◽  
Tyrosinase Expression ◽  
Hmb 45

Abstract Context.—Pathologists may encounter problems in the differential diagnosis of malignant melanoma, spindle and epithelioid neoplasms of peripheral nerves, and fibrohistiocytic tumors. Tyrosinase has been demonstrated to be a sensitive marker for melanoma. Objective.—To determine the specificity of tyrosinase expression in the differential diagnosis of melanoma, desmoplastic melanoma, and peripheral nerve sheath tumors. Design.—Immunoreactivity for tyrosinase, HMB-45 (anti-gp100 protein), S100 protein, CD34, and vimentin was studied in 70 tumors, including 15 melanomas (5 desmoplastic, 4 amelanotic, 6 melanotic), 13 malignant peripheral nerve sheath tumors; 10 schwannomas (1 pigmented), 12 neurofibromas (4 pigmented), and 20 fibrohistiocytic tumors (10 dermatofibrosarcoma protuberans and 10 dermatofibromas). Microwave-based antigen retrieval was performed in 10mM citrate buffer, pH 6.0, for 20 minutes at 121°C. Results.—All melanomas demonstrated positive immunostaining for tyrosinase, HMB-45, and S100 protein. Immunoreactivity for HMB-45 was generally stronger than that for tyrosinase in amelanotic lesions and significantly stronger in 1 of the desmoplastic lesions. The 4 pigmented neurofibromas were focally positive for tyrosinase, but did not stain for HMB-45. The pigmented schwannoma was focally positive for both tyrosinase and HMB-45. The malignant peripheral nerve sheath tumors, dermatofibrosarcoma protuberans, and dermatofibromas were nonreactive for tyrosinase and HMB-45. Conclusions.—Our results support the sensitivity of tyrosinase expression and demonstrate the relative specificity of tyrosinase as a marker for melanocytic lesions, including desmoplastic melanoma, although pigmented peripheral nerve tumors may demonstrate focal positive staining. Immunoreactivity for tyrosinase and HMB-45 may have been enhanced by the microwave-based antigen-retrieval technique used in this study.

scholarly journals PECOMA DE COLO UTERINO: UM RARO RELATO DE CASO

2021 ◽  
Vol 7 (3) ◽  
pp. 1-5
Author(s):  
Welington Lombardi ◽  
◽  
Luciana Borges Lombardi ◽  
Marina Abud Ferreira ◽  
Flávia Vicentin Silva ◽  
...  
Keyword(s):  
Hmb 45

Introdução: Os Pecomas são tumores de células epitelióides, de origem mesenquimal, normalmente benignos e extremamente raros. São encontrados mais frequentemente no retroperitônio, em região abdominopélvica, no trato gastrointestinal e no trato ginecológico, podendo ser encontrada também em tecidos moles e ossos. Recentemente foram publicados aproximadamente 100 casos de Pecoma, e cerca de 30% desses são de origem uterina, sendo o corpo do útero a região mais acometida. Em sua grande maioria possuem comportamento benigno, sendo raro o acometimento de estruturas ou órgãos vizinhos. Relato do caso: Trata-se de uma paciente de 38 anos, que apresentava sangramento uterino persistente, volume uterino aumentado e anemia associada. O ultrassom transvaginal mostrava a presença de vários nódulos sugestivos de leiomiomas. Após tratamento cirúrgico com histerectomia abdominal total, o anatomopatológico confirmou a presença de miomatose uterina, neoplasia intraepitelial cervical grau III e Pecoma de colo uterino. O exame de imunohistoquímica confirmou o diagnóstico de Pecoma, através da positividade para HMB-45, no achado cirúrgico. Conclusão: Portanto, por haver um número pequeno de relatos e estudos sobre o diagnóstico e tratamento do Pecoma, é importante e relevante estudarmos mais sobre essa afecção para melhorar os níveis de evidências e conhecimento sobre tal doença.

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