scholarly journals Evaluation of seven different drying treatments in respect to total flavonoid, phenolic, vitamin C content, chlorophyll, antioxidant activity and color of green tea (Camellia sinensis or C. assamica) leaves

2015 ◽  
Vol 53 (1) ◽  
pp. 721-729 ◽  
Author(s):  
Sahar Roshanak ◽  
Mehdi Rahimmalek ◽  
Sayed Amir Hossein Goli
2018 ◽  
Vol 23 (3) ◽  
pp. 124
Author(s):  
Fransiska Lisa Anindya Putri ◽  
Akhmad Kharis Nugroho ◽  
Erna Prawita Setyowati

Green tea (Camellia sinensis L.) is known to have ability to protect skin against free radicals. This is supported by polyphenol compound catechin. This research aims to determine the optimum Hydrophilic-Lipophilic Balance (HLB) value of Tween 60 and Span 80 compositions on the optimum cream formula of ethanol extract of green tea leaves. Tea leaves are extracted by macerating using 70% ethanol. Catechin in extract is known from Thin Layer Chromatography (TLC) test with silica gel 60 F254 as stationary phase and ethyl acetate:aquadest:formic acid (18:1:1 v/v) as mobile phase. Antioxidant activity is determined by 2,2-Diphenyl-1-picryhydrazyl (DPPH) method and value of Inhibition Concentration 50% (IC50) is then calculated. Formula optimization using Design Expert® version 7.1.5 (DX 7) software, Simplex Lattice Design (SLD) method with two components Tween 60 and Span 80. Cream is characterized according to physical properties organoleptic, homogeneity, viscosity, pH, spreadability, adhesiveness, and cream type. The optimum formula obtained is then tested for physical stability for 4 weeks at room temperature (28±2°C) and data are statistically analyzed using one-way ANOVA. The extract contains catechin proved with Retention factor (Rf) value 0.8 and has antioxidant activity with IC50 value 56.35 ppm. 6.4% Tween 60 and 3.6% Span 80 result an optimum HLB value 11.1. It has viscosity 2897.50±35.94 mPa.s, spreadability 18.44±0.06 cm2, adhesiveness 0.85±0.05 seconds, and pH 4.530±0.002. Statistical test shows that the cream is significantly altered at pH, but does not significantly change in viscosity, spreadability, and adhesiveness after being stored for 4 weeks.


2019 ◽  
Vol 10 (2) ◽  
pp. 119-128
Author(s):  
Desi Nawangsari

Abstract Green tea (Camellia sinensis L.) has the main efficacy as an antioxidant and contains polyphenol compounds in green tea leaves with the main component of polyphenol fraction namely Epigallocatechin-3-gallate (EGCG) which has strong activity to prevent free radicals that cause premature aging. Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.


2019 ◽  
Vol 10 (2) ◽  
pp. 129-134
Author(s):  
Desi Nawangsari

Abstract   Green tea (Camellia sinensis L.) has the main efficacy as an antioxidant and contains polyphenol compounds in green tea leaves with the main component of polyphenol fraction namely Epigallocatechin-3-gallate (EGCG) which has strong activity to prevent free radicals that cause premature aging. Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.


2019 ◽  
Vol 11 (4) ◽  
pp. 771-776
Author(s):  
Karmika Indarti ◽  
Elsa Fitria Apriani ◽  
Agung Eru Wibowo ◽  
Partomuan Simanjuntak

Author(s):  
Lucilene Dornelles Mello ◽  
Tiele Garcia Tunes

Objective: This study estimated the Permangante Index (IKMnO4) and evaluated its correlation with parameters as total phenols (FT) and total antioxidant activity (TAA) of commercial teas of Camellia sinensis, fermented, unfermented and blended with other plants.Methods: The aqueous extracts were prepared from commercial samples of green and black teas, green tea blended with lemon (Camellia sinensis with Citrus limonium), green tea blended with mint (Camellia sinensis with Mentha piperita), green tea blended with peach (Camellia sinensis with Prunus persica) and green tea blended with orange (Camellia sinensis with Citrus sinensis). The Permangante index was determined by titrating the tea extract with potassium permanganate solution according to AOAC method (AOAC, 1980) with modifications. The concentration of phenols in the extracts was determined by spectrophotometry according to the standard procedure of Folin-Ciocalteau. The antioxidant activity of the extracts was evaluated using the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH—) method. All the determinations were performed in triplicate, and the results presented as mean ± standard deviation (SD). The data were evaluated by univariate statistical analysis (ANOVA), F-test and mean test (Tukey´s test) for comparative results. Pearson’s correlation was used to measure the degree of linear correlation and the quantitative variables were from a normal population.Results: The results for Permanganate Index show a variation ranged from 0.40 to 0.80 mEq/l. The ANOVA analysis to IKMnO4 shown there is significant statistically difference at the 5% probability level (p < 0.05) between the types of tea as well as the amount of herb used to prepare the infusion. Therefore, it was observed that there significant contrast between tea extracts, the mixture of different plants in blended extracts as well as the tea manufacture process affect the polyphenols tannins content influencing the permanganate index parameter. Studies correlation shown strong correlations (r > 0.7) among the parameters. For the Camellia sinensis extracts the high correlations between IKMnO4 and TAA; FT and TAA demonstrates the suitability of the permanganate index in the evaluation of antioxidant activity.Conclusion: The results suggest that the Permanganate Index could be used for quality control of tea as an additional parameter.


2015 ◽  
Vol 14 ◽  
pp. 232-238 ◽  
Author(s):  
Lia Kusmita ◽  
Ika Puspitaningrum ◽  
Leenawaty Limantara

2021 ◽  
Vol 10 (1) ◽  
pp. 67
Author(s):  
Maria Christinela Domithesa ◽  
I Nengah Kencana Putra ◽  
Anak Agung Istri Sri Wiadnyani

This study aims to determine the effect of various solvent towards antioxidant activity of kejompot (crassocephalum crepioides) extract and to know the right solvent to obtained extract with the highest antioxidant activity. This study used a Completely Randomized Design Method with 4 treatment (water, methanol 70%, ethanol 70%, acetone 70%,) using maseration method. The treatment was repeated four times to obtain 16 experimental units. If there was a significant effect on the observed parameters, then followed by Duncan multipple range test. The result showed that the treatment significantly effected (P<0.05) on each of parameters such as : yield, tanin total, flavonoid, vitamin C, antioxidant activity. This study showed that methanol 70% as the best treatment that obtained yield, tannin, flavonoid, Vitamin C and antioxidant activity respectively 18.76 %, 1.05 mg TAE/g, 7.59 mg QE/g, 8.68 mg AAE/g, and 103.1 ppm antioxidant activity based on IC50  .


HortScience ◽  
2013 ◽  
Vol 48 (10) ◽  
pp. 1275-1282 ◽  
Author(s):  
Shiva Ram Bhandari ◽  
Bo-Deul Jung ◽  
Hum-Young Baek ◽  
Young-Sang Lee

To understand ripening-dependent changes in phytonutrients, five commercial cultivars of red peppers (Capsicum annuum L.) grown in an open field in Taean, South Korea, were selected and their fruits were harvested at green mature (GM), intermediate breaker (BR), and red ripe (RR) stages and their phytonutrient contents and antioxidant activities were compared. Three major patterns in relation to ripening progress were observed. First, continuous increases were observed in vitamin C, total phenol, vitamin E (especially α-tocopherol), total free sugar, β-carotene, linolenic acid content, and antioxidant activity. Second, decreasing patterns were observed in phytosterols (campesterol, stigmasterol, and β-sitosterol) and linoleic acid. Third, total flavonoid and squalene contents were relatively higher at the BR stage compared with the GM and RR stages. These results indicate that each phytonutrient has a unique pattern of accumulation and degradation during the fruit-maturing process. Unlike the mentioned phytonutrients, which showed similar patterns in all tested cultivars, capsaicinoids exhibited quite different patterns of ripening-dependent changes among the cultivars. Throughout the ripening processes, positive correlations with antioxidant activity were observed in vitamin E (r = 0.814**), β-carotene (r = 0.772*), vitamin C (r = 0.610**), and total phenol (r = 0.595**) contents, whereas capsaicinoids, total flavonoid, and phytosterols exhibited no or slightly negative correlations. In conclusion, the ripening of red pepper fruits is accompanied by continuous increments in various phytonutrients and subsequent antioxidant activity.


Sign in / Sign up

Export Citation Format

Share Document