Isolation and characterization of water-deficit stress-responsive α-expansin 1 (EXPA1) gene from Saccharum complex

Abstract Methyl chavicol and methyl eugenol are important phenylpropanoid compounds previously purified from basil. These compounds are significantly enhanced by the water deficit stress-dependent mechanism. Here, for the first time, pObCVOMT and pObEOMT promoters were extracted by the genome walking method. They were then cloned into the upstream of the β-glucuronidase (GUS) reporter gene to identify the pattern of GUS water deficit stress-specific expression. Histochemical GUS assays showed in transgenic tobacco lines bearing the GUS gene driven by pObCVOMT and pObEOMT promoters, GUS was strongly expressed under water deficit stress. qRT-PCR analysis of pObCVOMT and pObEOMT transgenic plants confirmed the histochemical assays, indicating that the GUS expression is also significantly induced and up-regulated by increasing density of water deficit stress. This indicates these promoters are able to drive inducible expression. The cis-acting elements analysis showed that the pObCVOMT and pObEOMT promoters contained dehydration or water deficit-related transcriptional control elements.

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Functional Characterization of Water-Deficit Stress Responsive Genes Using RNAi

Methods in Molecular Biology - Plant Stress Tolerance ◽

10.1007/978-1-60761-702-0_11 ◽

2010 ◽

pp. 193-206 ◽

Cited By ~ 2

Author(s):

Muthappa Senthil-Kumar ◽

Makarla Udayakumar ◽

Kirankumar S. Mysore

Keyword(s):

Water Deficit ◽

Functional Characterization ◽

Water Deficit Stress ◽

Stress Responsive Genes

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Genome-Wide Association Mapping through 90K SNP Array for Quality and Yield Attributes in Bread Wheat against Water-Deficit Conditions

Agriculture ◽

10.3390/agriculture10090392 ◽

2020 ◽

Vol 10 (9) ◽

pp. 392

Author(s):

Hafiz Ghulam Muhu-Din Ahmed ◽

Muhammad Sajjad ◽

Yawen Zeng ◽

Muhammad Iqbal ◽

Sultan Habibullah Khan ◽

...

Keyword(s):

Grain Yield ◽

Candidate Genes ◽

Water Deficit ◽

Bread Wheat ◽

Genome Wide Association ◽

Water Deficit Stress ◽

Yield Attributes ◽

Yield And Quality ◽

Isolation And Characterization ◽

Genome Wide

The decrease in water resources is a serious threat to food security world-wide. In this regard, a genome-wide association study (GWAS) was conducted to identify grain yield and quality-related genes/loci under normal and water-deficit conditions. Highly significant differences were exhibited among genotypes under both conditions for all studied traits. Water-deficit stress caused a reduction in grains yield and an increase in grains protein contents (GPC) and gluten contents (GLC). Population structure divided the 96 genotypes into four sub-populations. Out of 72 significant marker-trait associations (MTAs), 28 and 44 were observed under normal and water-deficit stress conditions, respectively. Pleiotropic loci (RAC875_s117925_244, BobWhite_c23828_341 and wsnp_CAP8_c334_304253) for yield and quality traits were identified on chromosomes 5A, 6B and 7B, respectively, under normal conditions. Under a water-deficit condition, the pleiotropic loci (Excalibur_c48047_90, Tdurum_contig100702_265 and BobWhite_c19429_95) for grain yield per plant (GYP), GPC and GLC were identified on chromosomes 3A, 4A and 7B, respectively. The pleiotropic loci (BS00063551_51 and RAC875_c28721_290) for GPC and GLC on chromosome 1B and 3A, respectively, were found under both conditions. Besides the validation of previously reported MTAs, some new MTAs were identified for flag leaf area (FLA), thousand grain weight (TGW), GYP, GPC and GLC under normal and water-deficit conditions. Twenty SNPs associated with the traits were mapped in the coding DNA sequence (CDS) of the respective candidate genes. The protein functions of the identified candidate genes were predicted and discussed. Isolation and characterization of the candidate genes, wherein, SNPs were mapped in CDS will result in discovering novel genes underpinning water-deficit tolerance in bread wheat.

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Corrigendum to “Functional characterization of three water deficit stress-induced genes in tobacco and Arabidopsis: An approach based on gene down regulation” [Plant Physiol. Biochem. 48 (2010) 35–44]

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2010.06.007 ◽

2010 ◽

Vol 48 (9) ◽

pp. 805

Author(s):

Muthappa Senthil-Kumar ◽

Ramanna Hema ◽

Thumu Rao Suryachandra ◽

H.V. Ramegowda ◽

Ramaswamy Gopalakrishna ◽

...

Keyword(s):

Water Deficit ◽

Functional Characterization ◽

Water Deficit Stress ◽

Down Regulation ◽

Induced Genes

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Characterization of physiological responses and fatty acid compositions of Camelina sativa genotypes under water deficit stress and symbiosis with Micrococcus yunnanensis

Symbiosis ◽

10.1007/s13199-020-00733-5 ◽

2020 ◽

Author(s):

Safoora Borzoo ◽

Sasan Mohsenzadeh ◽

Ali Moradshahi ◽

Danial Kahrizi ◽

Hajar Zamani ◽

...

Keyword(s):

Fatty Acid ◽

Water Deficit ◽

Physiological Responses ◽

Camelina Sativa ◽

Water Deficit Stress ◽

Fatty Acid Compositions

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Isolation and Characterization of Four Ascorbate Peroxidase cDNAs Responsive to Water Deficit in Cowpea Leaves

Annals of Botany ◽

10.1093/aob/mcj010 ◽

2005 ◽

Vol 97 (1) ◽

pp. 133-140 ◽

Cited By ~ 51

Author(s):

AGNÈS D'ARCY-LAMETA ◽

ROSELYNE FERRARI-ILIOU ◽

DOMINIQUE CONTOUR-ANSEL ◽

ANH-THU PHAM-THI ◽

YASMINE ZUILY-FODIL

Keyword(s):

Water Deficit ◽

Ascorbate Peroxidase ◽

Isolation And Characterization

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147 A 27-kDa Rhododendron Deacclimation Protein is Related to ABA Stress Ripening/Water Deficit Stress Inducible Family of Proteins

HortScience ◽

10.21273/hortsci.35.3.415a ◽

2000 ◽

Vol 35 (3) ◽

pp. 415A-415

Author(s):

Rajeev Arora ◽

Chon-Chong Lim

Keyword(s):

Amino Acids ◽

Water Deficit ◽

Aromatic Amino Acids ◽

Water Deficit Stress ◽

Compositional Bias ◽

Sds Page ◽

The Family ◽

Specific Proteins ◽

Controlled Freezing

Many reports have shown the accumulation of specific proteins associated with cold acclimation in plants. However, there is a scarcity of data on the physiological and/or biochemical changes associated with deacclimation process. This study was initiated to determine protein changes specifically associated with deacclimation in Rhododendron. Current-year leaves were collected from three Rhododendron cultivars (`Chionoides', `Grumpy Yellow', and `Vulcanís Flame'; ≈4-year-old rooted cuttings) during natural non-acclimated (June), cold-acclimated (January), and deacclimated (May) state. Leaf freezing tolerance was evaluated using controlled freezing protocol (Lim et al. 1998, J. Amer. Soc. Hort. Sci. 123:246–252). Seasonal SDS-PAGE profiles exhibited a distinct accumulation of 27 kDa protein in deacclimated and nonacclimated tissues, but this protein was essentially undetectable in cold acclimated tissues of all three cultivars. Further characterization of this polypeptide, labeled as RhDAP27 (for rhododendron deacclimation protein), revealed that it has an iso-electric point of 6.5, has a compositional bias for Glu/Gln (13.9%), His (11.4%), Gly (11%), Ala (10%), Lys (8.3%), and Asp/Asn (8.1%)—hydrophilic amino acids constitutedabout 54% of the total amino acids while 40% were nonpolar, aliphatic amino acids (Gly, Ala, Val, Leu, Ile, Pro) and only 6% were aromatic amino acids (Phe and Tyr). Micro-sequencing of the four peptides produced by partial cleavage of RhDAP27 revealed a striking homology of RhDAP27 with two proteins (from Mesembryanthemum crystallinum and Pinus taeda) that belong to the family of ABA stress ripening/water deficit stress inducible proteins.

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