Fat mass- and obesity-associated (FTO) gene promoted myoblast differentiation through the focal adhesion pathway in chicken

AbstractSeveral studies have shown that rs9939609 and rs1421085 in fat mass and obesity-associated (FTO) gene rs17782313 and rs12970134 in melanocortin-4 receptor (MC4R) gene influence obesity. In the present study, we aimed to determine association between rs9939609, rs1421085, rs17782313, and rs12970134 polymorphism, and their relation with body mass index (BMI), glucose, insulin, homeostasis model assessment of insulin resistance (HOMA-IR) and lipid values in obese children. We included 100 newly diagnosed obese children and 100 healthy children. The rs1421085 (CC/CT) (p = 0.019) and rs9939609 (AA/AT) (p = 0.002) polymorphism regions were higher in the obese group. Additionally, we found that both the rs1421085 (CC/CT) and rs9939609 (AA/AT) polymorphism associated with high-density lipoprotein cholesterol (p = 0.011 and p = 0.003) and triglycerides (p = 0.01 and p = 0.004) level, respectively. Further, the rs9939609 and rs1421085 variants of FTO gene associated with HDL-cholesterol and triglycerides levels in obese children; however, updated studies with a large sample size are required to establish strong links with genetic variants and risk factors in childhood obesity.

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Cytotoxic Effect of RecombinantMycobacterium tuberculosisCFP-10/ESAT-6 Protein on the Crucial Pathways of WI-38 Cells

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/917084 ◽

2009 ◽

Vol 2009 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Kun-Nan Tsai ◽

Err-Cheng Chan ◽

Tsung-Yeh Tsai ◽

Kuei-Tien Chen ◽

Chun-Yu Chen ◽

...

Keyword(s):

Focal Adhesion ◽

Microarray Data ◽

Cytotoxic Effect ◽

Time Course ◽

Essential Role ◽

Topological Properties ◽

Regulatory Effect ◽

Qrt Pcr ◽

Focal Adhesion Pathway ◽

Pathway Databases

To unravel the cytotoxic effect of the recombinant CFP-10/ESAT-6 protein (rCFES) on WI-38 cells, an integrative analysis approach, combining time-course microarray data and annotated pathway databases, was proposed with the emphasis on identifying the potentially crucial pathways. The potentially crucial pathways were selected based on a composite criterion characterizing the average significance and topological properties of important genes. The analysis results suggested that the regulatory effect of rCFES was at least involved in cell proliferation, cell motility, cell survival, and metabolisms of WI-38 cells. The survivability of WI-38 cells, in particular, was significantly decreased to 62% with 12.5 μMrCFES. Furthermore, the focal adhesion pathway was identified as the potentially most-crucial pathway and 58 of 65 important genes in this pathway were downregulated by rCFES treatment. Using qRT-PCR, we have confirmed the changes in the expression levels of LAMA4, PIK3R3, BIRC3, and NFKBIA, suggesting that these proteins may play an essential role in the cytotoxic process in the rCFES-treated WI-38 cells.

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Danggui Buxue Tang promotes the adhesion and migration of bone marrow stromal cells via the focal adhesion pathway in vitro

Journal of Ethnopharmacology ◽

10.1016/j.jep.2018.11.018 ◽

2019 ◽

Vol 231 ◽

pp. 90-97 ◽

Cited By ~ 4

Author(s):

Huaben Bo ◽

Junhua He ◽

Xiao Wang ◽

Ruilan Du ◽

Haikang Bei ◽

...

Keyword(s):

Bone Marrow ◽

Focal Adhesion ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Danggui Buxue Tang ◽

And Migration ◽

Focal Adhesion Pathway

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Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

Veterinary World ◽

10.14202/vetworld.2019.1160-1166 ◽

2019 ◽

Vol 12 (7) ◽

pp. 1160-1166

Author(s):

Amalia Puji Rahayu ◽

Tety Hartatik ◽

Agung Purnomoadi ◽

Edy Kurnianto

Keyword(s):

Fatty Acid ◽

Single Nucleotide Polymorphisms ◽

Fat Mass ◽

Milk Protein ◽

Fatty Acid Synthase ◽

Nucleotide Polymorphisms ◽

Fat Percentage ◽

Fto Gene ◽

Milk Traits ◽

Single Nucleotide

Aim: The study aimed to identify fatty acid synthase (FASN), LOC514211, and fat mass and obesity-associated (FTO) gene polymorphisms and to investigate their associations with milk traits in an Indonesian-Holstein dairy cow population. Materials and Methods: A total of 100 Indonesian-Holstein cows consisting of 50 heads (0th generation; G0) and 50 heads of their daughters (1st generation; G1) were used. Polymerase chain reaction-restriction fragment length polymorphism was performed to genotype three single nucleotide polymorphisms: rs41919985 in the FASN gene, rs42688595 in the LOC514211 gene, and g.1371T>A in the FTO gene. Results: FASN rs41919985 was associated with milk protein percentage (p<0.05), FTO g.1371T>A was associated with milk fat percentage (p<0.05), and LOC514211 rs42688595 was not associated with any trait (p>0.05). Heterozygote variants showed a higher protein percentage for FASN and the highest fat percentage for FTO. These associations were consistent in the G0 and G1 populations. Conclusion: Our results indicate that the milk protein and fat percentages can be improved by increasing the frequency of the AG genotype of FASN and the AT genotype of FTO, respectively.

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