Mapping QTL for important seed traits in an interspecific F2 population of pigeonpea

The study of diversity in common bean was based on morphological and agronomical characteristics, differentiation of collected accessions by morphological and molecular markers, detection of genetic variation, and duplicates detection in bean landraces. The analysed 82 accessions of common bean (Phaseolus vulgaris L.) were collected in the Western andEastern Carpatien as landrace mixtures. Their seeds were segregated and pooled according to their characteristics; they were further multiplicated, and introduced into the collection. An extensive variation in plant and seed traits was discovered in thirty-three morphological and agronomical characteristics. Nevertheless, some of the accessions were identical in these characteristics. Cluster analysis grouped genotypes into two main branches, reflecting the growth type, seed size parameters, and thousand-seed weight. Molecular differentiation studies were performed by multilocus polymorphism detection in microsatellite and minisatellite DNA regions. Cluster analysis based on molecular data also grouped genotypes but no linkage to morphological traits was revealed. Bean accessions with very similar or identical morphological characters were clearly distinguished by DNA banding patterns. The presence of duplicates was excluded.  

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Analysis and Mapping of Gene Families Encoding β-1,3-Glucanases of Soybean

Genetics ◽

10.1093/genetics/153.1.445 ◽

1999 ◽

Vol 153 (1) ◽

pp. 445-452

Author(s):

Wei Jin ◽

Harry T Horner ◽

Reid G Palmer ◽

Randy C Shoemaker

Keyword(s):

Gene Families ◽

Mrna Levels ◽

Linkage Groups ◽

Flower Bud ◽

Cross Hybridization ◽

Coding Regions ◽

F2 Population ◽

Young Leaves ◽

Glycine Max L ◽

Stem Leaf

Abstract Oligonucleotide primers designed for conserved sequences from coding regions of β-1,3-glucanase genes from different species were used to amplify related sequences from soybean [Glycine max (L.) Merr.]. Sequencing and cross-hybridization of amplification products indicated that at least 12 classes of β-1,3-glucanase genes exist in the soybean. Members of classes mapped to 34 loci on five different linkage groups using an F2 population of 56 individuals. β-1,3-Glucanase genes are clustered onto regions of five linkage groups. Data suggest that more closely related genes are clustered together on one linkage group or on duplicated regions of linkage groups. Northern blot analyses performed on total RNA from root, stem, leaf, pod, flower bud, and hypocotyl using DNA probes for the different classes of β-1,3-glucanase genes revealed that the mRNA levels of all classes were low in young leaves. SGlu2, SGlu4, SGlu7, and SGlu12 mRNA were highly accumulated in young roots and hypocotyls. SGlu7 mRNA also accumulated in pods and flower buds.

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QTL Mapping for Gummy Stem Blight Resistance in Watermelon (Citrullus spp.)

Plants ◽

10.3390/plants10030500 ◽

2021 ◽

Vol 10 (3) ◽

pp. 500

Author(s):

Eun Su Lee ◽

Do-Sun Kim ◽

Sang Gyu Kim ◽

Yun-Chan Huh ◽

Chang-Gi Back ◽

...

Keyword(s):

Chromosome 8 ◽

Chromosome 6 ◽

Potential Candidate ◽

Stem Blight ◽

Gummy Stem Blight ◽

F2 Population ◽

Leaf Lesion ◽

Phenotypic Variations ◽

A Minor ◽

Selection Of

Watermelon (Citrulluslanatus) is an economically important fruit crop worldwide. Gummy stem blight (GSB) is one of the most damaging diseases encountered during watermelon cultivation. In the present study, we identified quantitative trait loci (QTLs) associated with GSB resistance in an F2 population derived from a cross between maternal-susceptible line ‘920533’ (C. lanatus) and the paternal-resistant line ‘PI 189225’ (C. amarus). The resistance of 178 F2 plants was assessed by two different evaluation methods, including leaf lesion (LL) and stem blight (SB). To analyze the QTLs associated with GSB resistance, a linkage map was constructed covering a total genetic distance of 1070.2 cM. QTL analysis detected three QTLs associated with GSB resistance on chromosome 8 and 6. Among them, two QTLs, qLL8.1 and qSB8.1 on chromosome 8 identified as major QTLs, explaining 10.5 and 10.0% of the phenotypic variations localizing at same area and sharing the same top markers for both LL and SB traits, respectively. A minor QTL, qSB6.1, explains 9.7% of phenotypic variations detected on chromosome 6 only for the SB trait. High-throughput markers were developed and validated for the selection of resistant QTLs using watermelon accessions, and commercial cultivars. Four potential candidate genes were predicted associated with GSB resistance based on the physical location of flanking markers on chromosome 8. These findings will be helpful for the development of watermelon cultivars resistant to GSB.

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