Development of tetra-primer amplicon refractory mutation system (T-ARMS) strategy for identification of the dark green mutant allele in tomato

3 Biotech ◽  
2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Arnab Mukherjee ◽  
Shirin Akhtar ◽  
Tirthartha Chattopadhyay
Keyword(s):  
Mutant Allele ◽  
Mutation System ◽  
Dark Green ◽  
Green Mutant

Genetic Variation in TNF-α, its Relation with Inflammatory Biomarkers and Susceptibility to Rheumatoid Arthritis

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Khadiga Ahmed Ismail
Keyword(s):  
Rheumatoid Arthritis ◽  
Serum Level ◽  
Functional Disability ◽  
Serum Levels ◽  
Amplification Refractory Mutation System ◽  
Reactive Protein ◽  
Tnf Α ◽  
Mutation System ◽  
Potential Factor ◽  
Factor Α

Background: Tumor necrosis Factor-α (TNF-α) is encoded and controlled by TNF-α gene, which is involved in rheumatoid arthritis (RA) susceptibility. This research aimed to identify genetic variations of TNF-α (G308A) and to establish its association with inflammatory markers in Rheumatoid Arthritis predisposition. Methods: In the present study, fifty RA patients and fifty volunteers were involved and evaluated for the C-reactive protein, rheumatoid factor, and TNF-α were estimated by ELISA, Erythrocyte Sedimentation Rate (ESR) by Wintergreen method and for TNF-α-308 G>A polymorphism by polymerase chain reaction with amplification refractory mutation system (PCR-ARMS). Results: The CRP, RF, ESR and TNF-α were significantly elevated in RA patients relative to controls. The serum level TNF-α was also significantly elevated in female patients and in patients ≥50 years. Analysis of TNF-308 gene polymorphism revealed that GG genotypes were more prevalent in RA patients than in the healthy individuals and that GG genotype may be a potential factor to RA. The G allele was more common in RA than in the control. Elevated TNF-α serum levels were significantly associated the GG genotype and functional disability in RA patients. Conclusion: TNF-α promoter 308polymorphism GG genotype may be considered as a risk factor for RA and the TNF-α serum level was significantly related to the functional disability in the disease.

INTERRELATION OF ALLELIC VARIANTS OF HEMOSTASIS SYSTEM GENES ON THE DEVELOPMENT AND COURSE OF LUPUS NEPHRITIS

2019 ◽  
Vol 23 (2) ◽  
pp. 77-81
Author(s):  
E. N. Borisov ◽  
L. V. Ivanitsky ◽  
L. M. Samokhodskaya ◽  
T. N. Krasnova ◽  
E. P. Pavlikova ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Mutant Allele ◽  
Impaired Renal Function ◽  
Mthfr C677t ◽  
Mthfr Gene ◽  
Renal Survival ◽  
Mutant Genotype ◽  
Hemostatic System ◽  
Allelic Variations ◽  
Pai 1

THE AIM: to evaluate the effect of allelic variations in the hemostatic system genes on the development and course of lupus nephritis. PATIENTS AND METHODS. The study analyzed 100 patients with SLE Caucasians. 80 women and 20 men aged 16 to 73 years (mean age 37, ± 14 years). The duration of observation was for 73 patients over 5 years, for 18 – from 1 year to 5 years and for 9 – less than 1 year A rise in the level of creatinine in the blood above or equal to 2 mg / dl was considered a significant sign of impaired renal function. RESULTS. Among the patients included in the study, kidney damage was detected in 61 people (61%). In 33 of them (54.1%), a variant of renal pathology was observed according to the type of rapidly progressive lupus nephritis (BPVN). In patients with BH, mutations in the MTHFR (C677T) gene were statistically significantly more frequent (p = 0.033). The OR for the mutant genotype is 6.146 with 95% CI from 1.692 to 22.326. In patients with PWHD, mutations in the MTHFR (C677T) gene were statistically significantly more frequent (p = 0.031). The OR for the mutant genotype is 1.625 with 95% CI from 1.034 to 4.771. The five-year renal survival in carriers of the mutant allele of the MTHFR gene (C677T) is statistically significantly lower (72.8%) than in patients without this mutation (81.9%) (p = 0.027). Ten-year renal survival in carriers of the mutant allele of the MTHFR gene (C677T) is statistically significantly less (55.6%) than in patients without this mutation (70.5%) (p = 0.016). In patients with BH, mutations in the PAI-1 gene (4G / 5G 675) were statistically significantly more frequent (p = 0.046). OR for mutant genotype – 1.766 with 95% CI from 1.061 to 4.758. CONCLUSION. The mutant alleles of the MTHFR (C677T) and PAI-1 (4G / 5G 675) genes are likely to be associated with the development of BH. Polymorphism of the MTHFR gene (C677T) is associated with an unfavorable course of HH.

Silene lulakabadensis (Caryophyllaceae), a New Species from Iran

2020 ◽  
Vol 28 (1) ◽  
pp. 24-28
Author(s):  
Mahnaz Heidari Rikan ◽  
Farrokh Ghahremaninejad ◽  
Mostafa Assadi
Keyword(s):  
New Species ◽  
Conservation Status ◽  
Green Plant ◽  
Critically Endangered ◽  
Rare Plant ◽  
Soil Slopes ◽  
Dark Green ◽  
A New Species

Silene lulakabadensis Heidari, F. Ghahrem. & Assadi is described as a new species from Zanjan Province, Iran. The new species is a dark green plant, perennial and woody at the base, that was collected on marl soil slopes at 2100 m. It is believed to be closely related to S. eriocalycina Boiss. from section Auriculatae (Boiss.) Schischk. but is a smaller plant, with much shorter internodes, and pinkish-white retuse to emarginate petals with very small or no scales. It is a very rare plant and its conservation status is assessed as Critically Endangered.

Influences of Growth Regulators and Cultivars on Callus and Shoot Production of Alfalfa

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 461f-462
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Shoot Organogenesis ◽  
Multiple Shoots ◽  
Apparent Difference ◽  
Shoot Production ◽  
Callus Initiation ◽  
Dark Green ◽  
Number Of Shoots ◽  
Alfalfa Seeds

Alfalfa seeds of Cimarron VR, CW1446, CW2440, C94-118, C94-785, and WL311 were used as explants. BA, zeatin, and TDZ were evaluated on callus initiation, development, and shoot production. Callus initiation and development toward shoot organogenesis were enhanced when BA was added in the culture medium. Calli produced from BA treatments were compact, solid, and dark green. Similar results were obtained when zeatin was added in the culture medium. However, no shoots were produced from such calli. Multiple shoots were produced directly from each individual explant when TDZ was added to culture medium. However, when higher concentration of TDZ was used, number of shoots per explant was decreased, and dwarf shoots were produced. No apparent difference on shoot production was observed among the cultivars tested so far. Data on number of shoots per explant from two of these cultivars need to be statistically analyzed.

Biological removal of algae in an integrated pond system

1995 ◽  
Vol 31 (12) ◽  
pp. 21-31 ◽  
Author(s):  
P. G. J. Meiring ◽  
R. A. Oellermann
Keyword(s):  
Adsorptive Capacity ◽  
Trickling Filter ◽  
Laboratory Findings ◽  
Oxidation Pond ◽  
Trickling Filters ◽  
Biological Removal ◽  
In Series ◽  
The Media ◽  
Oxidation Ponds ◽  
Dark Green

A system of oxidation ponds in series with a biological trickling filter is described. It was known that this arrangement was incapable of reducing effectively the levels of algae present in the pond liquid even though nitrification was effected because of autotrophic conditions prevailing in the trickling filters. This very low trophic level explained the lack of adsorptive capacity present. By shortcircuiting less than 10 percent of the effluent from a fully loaded primary facultative oxidation pond to the trickling filter, the autotrophuc nature or the film in the trickling filter was sufficiently shifted towards a heterotrophic state that had sufficient adsorptive capacity to retain the majority of the algae. It is concluded that the algae, although being absorbed, stay alive on the film and do not contribute significantly to the carbonaceous load on the trickling filter. Further more the algae, although secluded from all sunlight, actually partake in the purification process, producing an effluent which, unlike a normal humus tank effluent, is surprisingly sparkling clear. This significant observation appears to be in line with laboratory findings by others who, when they artificially immobilised certain species of algae and passed water over them, concluded that the algae retained the potential to remove certain compounds from the water. Conglomerates of biologically flocculated dark-green algae are scoured off the film (or sloughed off as part of the film) and, having been photosynthetically inactive for some days, tend not to float, but settle very rapidly. A very significantly aspect of this development is the great potential it has for practical application in developing countries. The algae sloughed off the media are easily thickened and available for ultimate recovery from the water phase without the addition of chemicals.

scholarly journals MULTIPLE ALLELE APPROACH TO THE STUDY OF GENES IN DROSOPHILA MELANOGASTER THAT ARE INVOLVED IN IMAGINAL DISC DEVELOPMENT

Genetics ◽  
1978 ◽  
Vol 89 (2) ◽  
pp. 355-370 ◽  
Author(s):  
Allen Shearn ◽  
Grafton Hersperger ◽  
Evelyn Hersperger ◽  
Ellen Steward Pentz ◽  
Paul Denker
Keyword(s):  
Drosophila Melanogaster ◽  
Phenotypic Variation ◽  
Mutant Allele ◽  
Imaginal Disc ◽  
Reference Allele ◽  
Multiple Allele ◽  
Significant Difference ◽  
Chromosome Mutations ◽  
Cold Sensitive

ABSTRACT The phenotypes of five different lethal mutants of Drosophila melanogaster that have small imaginal discs were analyzed in detail. From these results, we inferred whether or not the observed imaginal disc phenotype resulted exclusively from a primary imaginal disc defect in each mutant. To examine the validity of these inferences, we employed a multiple-allele method. Lethal alleles of the five third-chromosome mutations were identified by screening EMS-treated chromosomes for those which fail to complement with a chromosome containing all five reference mutations. Twenty-four mutants were isolated from 13,197 treated chromosomes. Each of the 24 was then tested for complementation with each of the five reference mutants. There was no significant difference in the mutation frequencies at these five loci. The stage of lethality and the imaginal disc morphology of each mutant allele were compared to those of its reference allele in order to examine the range of defects to be found among lethal alleles of each locus. In addition, hybrids of the alleles were examined for intracistronic complementation. For two of the five loci, we detected no significant phenotypic variation among lethal alleles. We infer that each of the mutant alleles at these two loci cause expression of the null activity phenotype. However, for the three other loci, we did detect significant phenotypic variation among lethal alleles. In fact, one of the mutant alleles at each of these three loci causes no detectable imaginal disc defect. This demonstrates that attempting to assess the developmental role of a gene by studying a single mutant allele may lead to erroneous conclusions. As a byproduct of the mutagenesis procedure, we have isolated two dominant, cold-sensitive mutants.

Sign in / Sign up

Export Citation Format

Share Document