Elicitor-mediated enhancement of biomass, polyphenols, mangiferin production and antioxidant activities in callus cultures of Salacia chinensis L.

3 Biotech ◽  
2021 ◽  
Vol 11 (6) ◽  
Author(s):  
Jaykumar J. Chavan ◽  
Parthraj R. Kshirsagar ◽  
Sharad G. Jadhav ◽  
Virdhaval M. Nalavade ◽  
Swati T. Gurme ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Callus Cultures

scholarly journals Total Polyphenol Content, in vitro Antifungal and Antioxidant Activities of Callus Cultures from Inula crithmoides

2013 ◽  
Vol 8 (11) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Anahi Bucchini ◽  
Laura Giamperi ◽  
Donata Ricci
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Leaf Explants ◽  
Basal Medium ◽  
Alternaria Solani ◽  
Antimicrobial Activities ◽  
Callus Cultures ◽  
Naphthaleneacetic Acid ◽  
Stable Free Radical

This is the first report on the antioxidant and antifungal activities of callus cultures from Inula crithmoides L. (Asteraceae). Callus cultures were initiated from leaf sections, on initial culture MS basal medium supplemented with various concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid), NAA (1-naphthaleneacetic acid) and IBA (indole-3-butyric acid) and a 72% survival was achieved. Significant differences between the various auxins used as phytohormones on callus growth were found. Maximum callusing was noticed on the leaf explants grown on MS basal medium supplemented with 1 mgL–1 2,4-D. Subsequently the antioxidant and antimicrobial activities of the methanol extract from calli were investigated. Antioxidant studies suggested that the methanol extracts of dark-grown and light-grown callus were able to reduce the stable free radical 2,2-diphenyl-1-picrilhydrazyl (DPPH). In the inhibition against lipid peroxidation, extracts of dark-grown callus showed the strongest effect with IC50 values better than those of the standards. The methanol extract of callus cultures had significant antifungal activity only against two of the fungi tested: Alternaria solani and Phytophthora cryptogea. Against all the other tested fungi, the I. crithmoides calli extracts showed fungistatic activity.

scholarly journals Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)

2021 ◽  
Vol 10 (2) ◽  
pp. 79-94
Author(s):  
Farouk K. El-Baz ◽  
Amal A. Mohamed ◽  
Sami I. Ali
Keyword(s):  
Total Phenolics ◽  
Antioxidant Activities ◽  
Callus Formation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Callus Cultures ◽  
Scavenging Activity ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Callus cultures from stems, leaves and roots of colocynth were initiated on MS media supplemented with various combinations of 2,4 dichlorophenoxyacetic acid (2,4-D) with kinetin (KIN) and benzyladenine (BA) with α-naphthaleneacetic acid (NAA). The highest percentage of callus formation frequency (98.9%) was obtained from stem explants grown on MS media supplemented with (1.0 mg/L) 2,4-D + (1.0 mg/L) KIN. The total phenolics and flavonoid content of the colocynth callus cultures were measured. The results showed that the MS medium supplemented with 6.0 mg/L 2,4-D + 2.0 mg/L KIN (MD3) gave the highest content of total phenolics (19.2 mg/100g d.w.) in leaf-derived calli. The highest content of flavonoids (47.3 mg/100g d.w.) was obtained in stem derived calli grown on the same medium (MD3). Antioxidant activities of extracts were determined using different assays, including DPPH radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity and ferric reducing power. Leaf-derived calli cultured on MS medium + 2.0 mg/L 2,4-D + 1.0 mg/L KIN (MD1) showed the highest DPPH radical scavenging activity (85.3%). The highest percentage of H2O2 scavenging activity (61.4%) was detected in leaf explant-derived calli growing on MD1. The leaf-derived calli growing on (MD3) gave the highest ferric reducing power (22.3 μg/g d.w.), compared to the activities of stems, leaves and roots of in vitro grown seedlings (3.28, 12.9 and 2.85 μg/g d.w.), which were used as controls. On the basis of the current findings, we conclude that MS media supplemented with different combinations of 2,4-D and KIN yields higher phenolics, flavonoids contents and antioxidant activities than MS media supplemented with BA and NAA.

scholarly journals RAPID IN VITRO CALLOGENESIS AND PHYTOCHEMICAL SCREENING OF LEAF, STEM AND LEAF CALLUS OF MUSSAENDA FRONDOSA LINN. - A MEDICINAL PLANT.

2017 ◽  
Vol 10 (6) ◽  
pp. 81 ◽  
Author(s):  
Manasa Dj ◽  
Chandrashekar Kr ◽  
Bhagya N
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Leaf Explants ◽  
Reducing Power ◽  
Callus Cultures ◽  
Phytochemical Analysis ◽  
Qualitative And Quantitative

Objective: To standardise the protocol for rapid callogenesis in Mussaenda frondosa L. using leaf explants. Qualitative and quantitative phytochemical analysis of leaf, stem and callus cultures.Methods: The leaf explants were inoculated onto MS medium supplemented with varying concentrations of growth regulators such as 2, 4 - D, NAA, BAP, Kn for the induction of callus. Qualitative and quantitative analysis of total phenol, flavonoids and alkaloids contents of leaf, stem and callus were tested by standard methods.  The antioxidant activities were investigated using DPPH radical scavenging method and reducing power assay. The anti - inflammatory activity was evaluated by membrane stabilizing activity.Results: Pale green, healthy, friable and fast growing callus was obtained on the medium enriched with NAA (2mg/l) + Kn (4mg/l). Quantitative determination showed the highest concentration of total phenolics in the methanolic extract of in vitro grown callus (10 ± 1.1 mg of GA/g of extract), flavonoids in methanolic stem extract (137±1.6 mg of Quercitin/g of extract) and alkaloids in methanolic extract of leaf (118.3±1.5 mg/10g of extract). The methanolic leaf extract exhibited highest free radical scavenging activity with IC50 value of 40.6±10.06 μg/ml. The highest membrane stabilizing activity was shown by chloroform extract of the leaf (66.02%).Conclusion: The present preliminary phytochemical and pharmacological analysis may form the basis for drug development in future using callus cultures of M. frondosa.   

scholarly journals Polyphenol contents and antioxidant activity of carob callus cultures

2021 ◽  
pp. e652
Author(s):  
Assia Lozzi ◽  
Rachid Mentag ◽  
Driss Alami-Halimi ◽  
Rabha Abdelwahd ◽  
Abdelhadi Abousalim
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Correlation Coefficients ◽  
Biomass Accumulation ◽  
Callus Cultures ◽  
Lower Amount ◽  
Medicinal Uses ◽  
Light Regimes ◽  
Enhanced Production ◽  
Mediterranean Plant

Carob (Ceratonia siliqua L.) is an important Mediterranean plant species with worldwide commercial and medicinal uses. The establishment of a callus culture protocol as an alternative system to produce polyphenols of chemical and pharmaceutical interest was made in the present study for the first time in carob. Explant type and the light regime are two important factors that influence morphogenic responses and biochemical production. Maximal callus induction (100 %) and biomass accumulation were obtained in cotyledon explants under both tested light regimes (16-hour photoperiod and darkness). However, leaf callus produced a higher amount of polyphenols (TPC) and flavonoids (TFC) but a lower amount of total condensed tannins (TCT) as compared to cotyledon callus. Light treatment has significantly increased TCT content but decreased the antioxidant activity in carob callus cultures. Strong and positive correlations were obtained between TPC and TFC and the antioxidant activities with correlation coefficients ranging between 0.68 and 0.98. The obtained results indicate that calli of C. siliqua have the potential for enhanced production of phenolic compounds with antioxidant activity that is favored by culture under dark condition.

scholarly journals Polyphenols profiles and antioxidant activities of extracts from Capsicum chinense in vitro plants and callus cultures

2019 ◽  
Vol 2 (1) ◽  
pp. 30 ◽  
Author(s):  
Gergana Sherova ◽  
Atanas Pavlov ◽  
Vasil Georgiev
Keyword(s):  
Callus Culture ◽  
Antioxidant Activities ◽  
Sinapic Acid ◽  
Callus Cultures ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Capsicum Chinense ◽  
Practical Applications ◽  
In Vitro Plants

In vitro plants of Capsicum chinense cv. Carolina Reaper have been obtained by planting seeds on sterile MS medium. The plants were used to initiate callus culture on half strength MS medium, supplied with 2 mg/l 1-Naphthaleneacetic acid and 0.5 mg/L 6-Benzylaminopurine. The polyphenol profiles (phenolic acids and flavonoids) of methanol extracts from obtained callus and in vitro plants have been analyzed by HPLC. The main constituents in plant extract were protocatechuic acid, sinapic acid, rutin, hesperetin and myricetin, whereas in callus extract the major compounds were found to be chlorogenic acid, ferulic acid, rutin, hyperoside, myricetin and hesperetin. The antioxidant capacity of both extracts have been evaluated by using DPPH, TEAC, FRAP and CUPRAC assays. In our knowledge, this is the first report for obtaining of callus culture from Capsicum chinense cv. Carolina Reaper and evaluation of phytochemical profiles and antioxidant activities of its extract. Practical applications: The research outlines the potential of Capsicum in vitro systems as a renewable source of active ingredients for application in cosmetic and food products.

Evaluation of Growth and Antioxidant Activity in Suaeda monoica and Suaeda nudiflora Callus Cultures under Sequential Exposure to Saline Conditions

2019 ◽  
Vol 8 (1) ◽  
pp. 42-52
Author(s):  
Abhishek Joshi ◽  
Bhanupriya Kanthaliya ◽  
Jaya Arora
Keyword(s):  
Antioxidant Activity ◽  
Callus Culture ◽  
Antioxidant Activities ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Specific Cell ◽  
Strong Positive Correlation ◽  
Ms Medium ◽  
Callus Regeneration

Background: Plant in vitro culture systems serve as a useful tool to study the regulatory routes which are related to plant growth and survival under altered environmental conditions. Methods: Callus culture of Suaeda monoica and Suaeda nudiflora were established for studying the salt tolerance mechanism at the cellular level. Calli of both the species were induced from seedling’s epicotyls on Murashige and Skoog (MS) medium supplemented with a different combination of auxin and cytokinins. A sequential stress treatment was given to the callus of both the species. The growth rate of callus, osmolytes and antioxidant activities was investigated after 28 days. A control callus was maintained in each experiment without any salt in the growth medium. Results: Efficient callus regeneration was obtained by exposing the callus tissue to MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l), benzylaminopurine (BAP, 0.5 mg/l) and 2,4-D (0.5 mg/l), kinetin (Kn, 0.25 mg/l) for S. monoica and S. nudiflora, respectively. A substantial increase was observed in proline content and a strong positive correlation was found between the total phenolic content and antioxidant activity under increasing salt concentrations. Conclusion: This is the first report on S. monoica callus regeneration. The specific cell lines which were generated through callus culture under sequential saline conditions provide a promising foundation for studying salinity induced expression of enzymes. Further comparison of transcriptomic profiles of control and salt-treated callus cultures can serve as a promising system for the detection of genes responsible for the change in expression under salt stress.

SEM and fluorescence imaging of callose deposition in root hair tips and suspension cultures of Streptanthus tortuosus

1990 ◽  
Vol 48 (3) ◽  
pp. 698-699
Author(s):  
K.S. Walters ◽  
R.D. Sjolund ◽  
K.C. Moore
Keyword(s):  
Cell Wall ◽  
Root Hair ◽  
Cultured Cells ◽  
Root Hairs ◽  
Callus Cultures ◽  
Callose Deposition ◽  
Culture Cells ◽  
Wall Structures ◽  
Ultraviolet Illumination ◽  
Callose Formation

Callose, B-1,3-glucan, a component of cell walls, is associated with phloem sieve plates, plasmodesmata, and other cell wall structures that are formed in response to wounding or infection. Callose reacts with aniline blue to form a fluorescent complex that can be recognized in the light microscope with ultraviolet illumination. We have identified callose in cell wall protuberances that are formed spontaneously in suspension-cultured cells of S. tortuosus and in the tips of root hairs formed in sterile callus cultures of S. tortuosus. Callose deposits in root hairs are restricted to root hair tips which appear to be damaged or deformed, while normal root hair tips lack callose deposits. The callose deposits found in suspension culture cells are restricted to regions where unusual outgrowths or protuberances are formed on the cell surfaces, specifically regions that are the sites of new cell wall formation.Callose formation has been shown to be regulated by intracellular calcium levels.

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