Polyphenol contents and antioxidant activity of carob callus cultures

Carob (Ceratonia siliqua L.) is an important Mediterranean plant species with worldwide commercial and medicinal uses. The establishment of a callus culture protocol as an alternative system to produce polyphenols of chemical and pharmaceutical interest was made in the present study for the first time in carob. Explant type and the light regime are two important factors that influence morphogenic responses and biochemical production. Maximal callus induction (100 %) and biomass accumulation were obtained in cotyledon explants under both tested light regimes (16-hour photoperiod and darkness). However, leaf callus produced a higher amount of polyphenols (TPC) and flavonoids (TFC) but a lower amount of total condensed tannins (TCT) as compared to cotyledon callus. Light treatment has significantly increased TCT content but decreased the antioxidant activity in carob callus cultures. Strong and positive correlations were obtained between TPC and TFC and the antioxidant activities with correlation coefficients ranging between 0.68 and 0.98. The obtained results indicate that calli of C. siliqua have the potential for enhanced production of phenolic compounds with antioxidant activity that is favored by culture under dark condition.

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Green synthesized silver and copper nanoparticles induced changes in biomass parameters, secondary metabolites production, and antioxidant activity in callus cultures of Artemisia absinthium L.

Green Processing and Synthesis ◽

10.1515/gps-2021-0010 ◽

2021 ◽

Vol 10 (1) ◽

pp. 61-72

Author(s):

Khizar Hayat ◽

Shahid Ali ◽

Saif Ullah ◽

Yujie Fu ◽

Mubashir Hussain

Keyword(s):

Antioxidant Activity ◽

Copper Nanoparticles ◽

Biomass Accumulation ◽

Callus Cultures ◽

Total Phenolic ◽

Ag Nps ◽

Artemisia Absinthium ◽

Culture Production ◽

Metabolites Production ◽

Induced Changes

Abstract Artemisia absinthium L. is a highly medicinal plant with a broad range of biomedical applications. A. absinthium callus cultures were established in response to bio-fabricated single NPs (Ag and Cu) or a combination of both NPs (Ag and Cu) in different ratios (1:2, 2:1, 1:3, and 3:1) along with thidiazuron (TDZ) (4 mg/L) to elicit the biomass accumulation, production of non-enzymatic compounds, antioxidative enzymes, and antioxidant activity. Silver and copper nanoparticles (Ag and Cu NPs) were synthesized using the leaves of Moringa oleifera as reducing and capping agent and further characterized through UV-Visible spectroscopy and SEM. The 30 µg/L suspension of Ag and Cu NPs (1:2, 2:1) and 4 mg/L TDZ showed 100% biomass accumulation as compared to control (86%). TDZ in combination with Ag NPs enhanced biomass in the log phases of growth kinetics. The Cu NPs alone enhanced the superoxide dismutase activity (0.56 nM/min/mg FW) and peroxidase activity (0.31 nM/min/mg FW) in callus cultures. However, the combination of Ag and Cu NPs with TDZ induced significant total phenolic (7.31 µg/g DW) and flavonoid contents (9.27 µg/g DW). Furthermore, the antioxidant activity was highest (86%) in the Ag and Cu NPs (3:1) augmented media. The present study provides the first evidence of bio-fabricated single NPs (Ag and Cu) or a combination of both NPs (Ag and Cu) in different ratios (1:2, 2:1, 1:3, and 3:1) along with TDZ (4 mg/L) on the development of callus culture, production of endogenous enzymes, non-enzymatic components, and further antioxidant activity in callus cultures of A. absinthium.

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Enhanced production of bioactive compound in the callus culture of Glycyrrhiza glabra L. with the improved biomass accumulation

10.21203/rs.3.rs-162959/v1 ◽

2021 ◽

Author(s):

Nancy Jaiswal ◽

Yashodhara Verma ◽

Pragati Misra

Keyword(s):

Callus Culture ◽

Biomass Accumulation ◽

Bioactive Compound ◽

Callus Cultures ◽

Glycyrrhiza Glabra ◽

Enhanced Production ◽

Adenine Sulphate ◽

Wide Range ◽

Metabolite Content

Abstract Licorice ( Glycyrrhiza glabra ) of Fabaceae family is known to have wide range of medicinal properties due to the metabolites found in the plant’s tissue. Callus cultures from Glycyrrhiza glabra were previously initiated in vitro using leaf as an explant. The current study was designed in a way to examine the possible role of different elicitors concentrations on the stimulation of biomass and their effects on different metabolite content such as total carbohydrates, protein, proline, phenol, alkaloid, flavonoid and glycyrrhizin. Elicitation with different concentration of elicitors increased the biomass and metabolite content in callus culture of G. glabra at different rates. The optimum concentration of adenine sulphate for maximum biomass accumulation (16.79 g/flask) was found to be at 50 mg/l on the incubation of 20 th days. Adenine sulphate as well as putrescine was also found to stimulate the different metabolite content to 3-4 folds in the callus cultures as compared to that of control. Results showed that the metabolite content and antioxidant enzyme of Glycyrrhiza glabra can be enhanced by appropriate forms of elicitation.

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Evaluation of Pigments, Phenolic and Volatile Compounds, and Antioxidant Activity of a Spontaneous Population of Portulaca oleracea L. Grown in Tunisia

Agriculture ◽

10.3390/agriculture10080353 ◽

2020 ◽

Vol 10 (8) ◽

pp. 353

Author(s):

Samia Dabbou ◽

Karima Lahbib ◽

Gaetano Pandino ◽

Sihem Dabbou ◽

Sara Lombardo

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Biochemical Characterization ◽

Antioxidant Activities ◽

Portulaca Oleracea ◽

Gas Chromatography Mass Spectrometry ◽

African Countries ◽

Potential Health ◽

Medicinal Uses ◽

Phytochemical Compounds

Portulaca oleracea L., commonly known as purslane, is a weed with worldwide distribution and considerable medicinal uses due to its high levels of phytochemical compounds. However, until now, few studies have been conducted on the biochemical characterization of P. oleracea grown in Tunisia, a possible area of its origin as other North African countries. Therefore, the aim of the present study was to determine the phytochemical composition and antioxidant potential of leaves and stems from a Tunisian spontaneous population of purslane. Particularly, samples were analyzed for their proximate composition, pigments, and volatiles, whereas ethanolic and aqueous extracts were evaluated for their composition in phenolic compounds and in vitro antioxidant activities. Stems showed higher content of moisture (89.9%) and anthocyanins (4.61 µg g−1 dry matter, DM), whereas leaves revealed higher chlorophyll concentrations (7.42 mg g−1 DM). Significantly higher levels of phenolic compounds and antioxidant capacities (p < 0.05) were obtained in ethanolic extracts, compared with water extracts, irrespective of the analyzed plant part. A high antioxidant activity of stems was obtained, especially when extracted with ethanol. Headspace gas chromatography-mass spectrometry (GC-MS) analyses revealed six volatile classes with monoterpene hydrocarbons, oxygenated monoterpenes, and non-terpene derivatives as the highly represented compounds. Limonene (17.3–32.2%), carvone (38–46%), 2,6-dimethylcyclohexanol (2.2–6.4%), and nonanal (3.4–3.8%) were the most abundant volatiles. Based on the results of the present study, Tunisian purslane should deserve major consideration as an edible vegetable due to its richness in phytochemical compounds and, hence, for its potential health effects.

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Evaluation of Growth and Antioxidant Activity in Suaeda monoica and Suaeda nudiflora Callus Cultures under Sequential Exposure to Saline Conditions

Current Biotechnology ◽

10.2174/2211550108666190507122304 ◽

2019 ◽

Vol 8 (1) ◽

pp. 42-52

Author(s):

Abhishek Joshi ◽

Bhanupriya Kanthaliya ◽

Jaya Arora

Keyword(s):

Antioxidant Activity ◽

Callus Culture ◽

Antioxidant Activities ◽

Callus Cultures ◽

Total Phenolic ◽

Specific Cell ◽

Strong Positive Correlation ◽

Ms Medium ◽

Callus Regeneration

Background: Plant in vitro culture systems serve as a useful tool to study the regulatory routes which are related to plant growth and survival under altered environmental conditions. Methods: Callus culture of Suaeda monoica and Suaeda nudiflora were established for studying the salt tolerance mechanism at the cellular level. Calli of both the species were induced from seedling’s epicotyls on Murashige and Skoog (MS) medium supplemented with a different combination of auxin and cytokinins. A sequential stress treatment was given to the callus of both the species. The growth rate of callus, osmolytes and antioxidant activities was investigated after 28 days. A control callus was maintained in each experiment without any salt in the growth medium. Results: Efficient callus regeneration was obtained by exposing the callus tissue to MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l), benzylaminopurine (BAP, 0.5 mg/l) and 2,4-D (0.5 mg/l), kinetin (Kn, 0.25 mg/l) for S. monoica and S. nudiflora, respectively. A substantial increase was observed in proline content and a strong positive correlation was found between the total phenolic content and antioxidant activity under increasing salt concentrations. Conclusion: This is the first report on S. monoica callus regeneration. The specific cell lines which were generated through callus culture under sequential saline conditions provide a promising foundation for studying salinity induced expression of enzymes. Further comparison of transcriptomic profiles of control and salt-treated callus cultures can serve as a promising system for the detection of genes responsible for the change in expression under salt stress.

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Chemical Composition and Antiproliferative and Antioxidant Activities of Methanolic Extract of Alcea setosa A. Malvaceae

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.01115 ◽

2021 ◽

pp. 6447-6454

Author(s):

Hana R. Bajes ◽

Sawsan A. Oran ◽

Yasser K. Bustanji

Keyword(s):

Antioxidant Activity ◽

Cancer Cells ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Human Fibroblasts ◽

Wild Plant ◽

Scavenging Activity ◽

Medicinal Uses ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

Alcea setosa A. (Malvaceae) is a wild plant that grows in Jordan and have several traditional medicinal uses. This study aims to collect and chemically analyze the methanolic extract from Alcea setosa A. from Jordan and to evaluate its cytotoxic and antioxidant activity against human breast cancer cells (T47D), colorectal adenocarcinoma cells (CACO2), and normal human fibroblasts (MRC5). The extract was extracted by methanol solvent and analyzed by liquid chromatography coupled with a mass spectrometer. Cell viability was assessed using trypan blue, neutral red, and MTT assays, and antioxidant activity was evaluated using DPPH scavenging activity assay. A total of 290 compounds, 12 among which were identified when compared to available standards, the extract contained six flavons derivatives, Two fatty acids, one ketone derivative, one flavonol derivative, one organic acid, and one coumarin derivative. The results also revealed that the IC50 values of the viability assays were higher among normal cells compared to the human cancer cell lines, and the viability inhibition was significant at higher concentrations compared to untreated cells. Nevertheless, moderate antioxidant activity was observed for the extract in the DPPH scavenging activity test. To sum up, this study indicates that samples of A. setosa collected from Jordan is likely to be an effective antioxidant, is optimistically potential to be utilized in breast and colon cancers treatment due to its preferential cytotoxicity against cancer cells.

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Antibacterial, Antifungal and Antioxidant activity of leaf and fruit of Alangium salviifolium L. (Cornaceae)

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00484 ◽

2021 ◽

pp. 2745-2749

Author(s):

Lavanya D ◽

Pooja Rao ◽

Soundarya S ◽

Surabhi T.S ◽

Prashith Kekuda T.R

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

Antioxidant Activities ◽

Diffusion Method ◽

Fruit Extract ◽

Medicinal Uses ◽

Fruit Extracts ◽

The Family ◽

Reducing Activity ◽

Antibacterial And Antifungal

Objectives: Alangium salviifolium L. is one of the medicinally important plant belonging to the family Cornaceae. The present study evaluates antibacterial, antifungal and antioxidant activity of leaf and fruit extracts of A. salviifolium. Methods: Extraction of shade dried and powdered leaf and fruit was carried out by maceration technique. Antibacterial and antifungal activity was evaluated by agar well diffusion method and poisoned food technique, respectively. Antioxidant activity was determined by antiradical (DPPH and ABTS assays) and ferric reducing assays. Results: Both leaf and fruit extracts displayed inhibitory activity against test bacteria. Fruit extract was shown to be more effective against test bacteria when compared to leaf extract. Leaf extract was more effective in causing inhibition of mycelial growth of test fungi when compared to fruit extract. Both leaf and fruit extracts scavenged DPPH and ABTS radicals dose dependently and exhibited ferric reducing activity. Conclusions: The results of the present study indicated the potential of leaf as well as fruit of A. salviifolium to exhibit antimicrobial and antioxidant activities which justifies the traditional medicinal uses of the plant.

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Determination of Antioxidant Activities and Total Phenolic Contents of Three Cyanobacterial Species

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.625.826 ◽

2014 ◽

Vol 625 ◽

pp. 826-829 ◽

Cited By ~ 2

Author(s):

Hamdy Elsayed Ahmed Ali ◽

Sanaa Mahmoud Metwally Shanab ◽

Mervat Aly Mohamed Abo-State ◽

Emad Ahmed Ahmed Shalaby ◽

Usama Mohamed Nour El Demerdash ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Water Extract ◽

Correlation Coefficients ◽

Ethanolic Extract ◽

Natural Antioxidants ◽

Total Phenolic ◽

Phenolic Contents ◽

Cyanobacterial Species ◽

Dpph Scavenging

The antioxidant activity and total phenolic content of different extracts (hexane, chloroform, ethyl acetate, ethanol (70%) and water) of three tested cyanobacterial species (Phormidium sp., Oscillatoria sp. and Nostoc muscorum ) were evaluated, using 2,2 ́-diphenyl-1-picrylhydrazyl (DPPH) scavenging method and Folin–Ciocalteu method, respectively. The ethanolic extract of Oscillatoria sp. possessed the highest antioxidant activity (69.13±0.35 %) and therefore could be potential source of natural antioxidants. The correlation coefficients between the antioxidant activities and the phenolic contents were very small in most of extracts (R2 = 0.0291-0.2934). However, the water extract demonstrated relatively higher value (R2= 0.7446). Thus, phenolic compounds may not be considered the major contributors for the antioxidant activities of these cyanobacterial species. So, other antioxidant substances may be present in the extract and share in this activity.

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Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000506 ◽

2017 ◽

Vol 87 (3-4) ◽

pp. 191-200 ◽

Cited By ~ 1

Author(s):

Nidhal Soualeh ◽

Aliçia Stiévenard ◽

Elie Baudelaire ◽

Rachid Soulimani ◽

Jaouad Bouayed

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Biological Activities ◽

Good Alternative ◽

Activity Levels ◽

Salix Alba ◽

Rosa Canina ◽

Free Process ◽

Plant Powders ◽

Sieving Process

Abstract. In this study, cytoprotective and antioxidant activities of Rosa canina (RC) and Salix alba (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, versus a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50–100 µm, 100–180 µm and 180–315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H2O2-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50–100 µm and 100–180 µm, both p < 0.001), and with the intermediate powder for RC (i. e., 100–180 µm, p < 0.05) versus HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H2O2, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.

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SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

Jurnal Natural ◽

10.30862/jn.v7i1.678 ◽

2008 ◽

Vol 7 (1) ◽

Author(s):

Bimo Budi Santoso

Keyword(s):

Antioxidant Activity ◽

Medicinal Plants ◽

Antitumor Activity ◽

Traditional Method ◽

Extraction Efficiency ◽

Phenolic Content ◽

Antioxidant Activities ◽

Total Phenolic ◽

Phenolic Contents ◽

Total Phenolic Contents

Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed. The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents, A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R2= 0,77) and methanol (R2 = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.

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Antioxidant activity of cookiessupplemented with sugarbeet dietary fiber

Sugar Industry ◽

10.36961/si11177 ◽

2011 ◽

pp. 151-157 ◽

Cited By ~ 2

Author(s):

Marijana B. Saka ◽

Julianna F. Gyura ◽

Aleksandra Mišan ◽

Zita I. Šereš ◽

Biljana S. Pajin ◽

...

Keyword(s):

Antioxidant Activity ◽

Shelf Life ◽

Dietary Fiber ◽

Wheat Flour ◽

Room Temperature ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Functional Characteristics

The antioxidant activity of cookies prepared by the addition of sugarbeet dietary fibers was investigated in order to estimate their influence on functional characteristics and shelf-life of cookies. Treated fiber (TF) was obtained from sugarbeet by extraction with sulfurous acid (75 °C at pH = 5.7during 60 min) and treatment with hydrogen peroxide (20 g/LH2O2 at pH = 11 during 24 h). The fiber obtained was dried (80 °C), ground and sieved. TF was investigated in comparison with commercially available Fibrex®. The cookies were prepared by the addition of 0, 7, 9 and 11% of sugarbeet dietary fiber as a substitute for wheat flour in the formulation of cookies. The antioxidant properties of cookies were tested every 7 days using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity test during 6 weeks of storage at room temperature (23 ± 1 ºC). The obtained results indicated that substitution of wheat flour with Fibrex® in the formulation of cookies upgraded the antioxidant activity, i.e. the functional characteristics of Fibrex®-enriched cookies and could prolong their shelf-life. In contrast, TF did not increase the antioxidant activity of TF-enriched cookies. The better antioxidant activities of Fibrex®-enriched cookies could be attributed to the presence of ferulic acid.

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