Effect of metals ions on thermostable alkaline phytase from Bacillus subtilis YCJS isolated from soybean rhizosphere soil

2013 ◽  
Vol 64 (3) ◽  
pp. 1123-1131 ◽  
Author(s):  
Ming-Ze Yao ◽  
Wen-Liang Lu ◽  
Ting-Gui Chen ◽  
Wei Wang ◽  
Yue-Jun Fu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Rhizosphere Soil ◽  
Alkaline Phytase

scholarly journals Complete Genome Sequence of Bacillus subtilis J-5, a Potential Biocontrol Agent

2017 ◽  
Vol 5 (23) ◽  
Author(s):  
Zhenhua Jia ◽  
Weiwei Jin ◽  
Yali Huang ◽  
Shuishan Song
Keyword(s):  
Bacillus Subtilis ◽  
Biocontrol Agent ◽  
Rhizosphere Soil ◽  
Whole Genome ◽  
Antimicrobial Compounds ◽  
Content Type ◽  
Regulatory Systems ◽  
Potential Biocontrol Agent ◽  
Genes Encoding ◽  
Shed Light

ABSTRACT Bacillus subtilis J-5 was isolated from tomato rhizosphere soil and exhibited strong inhibitory activity against Botrytis cinerea. To shed light on the molecular mechanism underlying the biological control on phytopathogens, the whole genome of this strain was sequenced. Genes encoding antimicrobial compounds and the regulatory systems were identified in the genome.

scholarly journals Variable survival ability of rhizobacteria in cumin (Cuminum cyminum L.) rhizosphere

2016 ◽  
Vol 8 (3) ◽  
pp. 1699-1703
Author(s):  
Anurag Yadav ◽  
Kusum Yadav
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Rhizosphere Soil ◽  
Population Decline ◽  
Dry Weight ◽  
Bulk Soil ◽  
Subtilis Strain ◽  
Initial Population ◽  
Final Population ◽  
Better Than

A study was undertaken to compare the survival efficacy of two native, previously characterized bacterial biovars viz. Bacillus subtilis BCU5 and Pseudomonas fluorescens PCU17 with Bacillus subtilis strain MTCC1789 and Pseudomonas fluorescens strain MTCC4828, procured from Institute of Microbial Technology, Chandigarh,India in cumin rhizosphere and bulk soil. All the four bacterial types were made rifampicin resistant and the mutants were applied as inoculants at the dosage of 6 log, 7 log and 8 log colony forming units (cfu) g-1 dry soil weight in pots containing cumin seedlings. The cfu of rhizosphere and bulk soil of pots was observed per week for four weeks. The results show that the initial population decline is a common feature of bioinoculants. In rhizosphere and bulk soil, the native bacterial biovars survived better than their procured counterparts. The population of P. fluorescens strain MTCC4828r in rhizosphere soil declined faster and reached below detection limit whereas the P. fluorescens biovar PCUr rhizosphere final population dropped to 3.1 log, 2.9 log and 2.13 log cfu g-1 soil dry weight with 8 log, 7 log and 6 log cfu g-1 soil dry weight inoculum treatment, respectively. In contrast to P. fluorescens strain MTCC4828r, the population of B. subtilis strain MTCC1789r stabilized after some decline and was comparable with B. subtilis biovar BCU5 population. Study concludes that the inoculant population decline in soil was the result of lower microbial load carrying capacity of soil than the provided inoculum densities. Also, the native bacteria survived better than procured ones in rhizosphere soil.

scholarly journals RHIZODEGRADATION OF PHENANTHRENE, ANTHRACENE AND PYRENE BY AUGMENTING BACILLUS CEREUS AND BACILLUS SUBTILIS STRAINS

2021 ◽  
Vol 9 (02) ◽  
pp. 89-96
Author(s):  
M. Poornachander Rao ◽  
◽  
Anitha Yerra ◽  
K. Satyaprasad ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Contaminated Soils ◽  
Rhizosphere Soil ◽  
Culture Method ◽  
Experimental Period ◽  
Bacterial Strains ◽  
Bacterial Populations ◽  
Metabolic Activities ◽  
Pot Culture

Rhizodegradation is one of the best methods for the effective removal of dangerous polycyclic aromatic hydrocarbons pollutants from soil. This is operative due to the high persistent, non-bioavailability nature of PAHs and combined, sequential reactions of bacteria present in rhizosphere of plants. We have conducted pot-culture method to study the degradation of three PAHs compounds namely phenanthrene, anthracene and pyrene in artificially contaminated soils of rhizosphere and non-rhizosphere soil treatments of blackgram(Vignamungo L.) that augmented by two potential PAHs degraders namely Bacillus cereus CPOU13 and Bacillus subtilis SPC14 isolated from naturally contaminated soils for 90days. HPLC studies revealed that degradation percentages of the three PAHs in treatments were more where selected strains augmented to the soil treatments over the non-augmented soils. The rhizosphere treatments that have augmented strains recorded more degradation percentages of phenanthrene, anthracene and pyrene over the rhizosphere treatments that were non-augmented. Pyrene, a high molecular weight PAHs degraded maximum to 96.24% in rhizosphere soil treatment that is augmented with the strains while moderate degradation of pyrene recorded in non-autoclaved soil treatments that contain natural microbial communities. The study of counting of bacterial populations during the experimental period revealed that the populations of the selected and other natural bacteria were gradually increased from the first day, reached maximum by 60days and became almost consistent in 90days in all the treatments. It was also observed that the populations of bacteria were high in rhizosphere treatments compared to the non-rhizosphere soil treatments. With these results it has been predicted that degradation of PAHs in rhizosphere soil treatments is closely associated with the increasing PAHs degrading bacterial populations of selected bacterial strains that may consume more quantity of PAHs for their metabolic activities in rhizosphere soils. Key words: Rhizodegradation, PAHs, HPLC, pot culture.

scholarly journals Characterization of two antimicrobial peptides produced by a halotolerant Bacillus subtilis strain SK.DU.4 isolated from a rhizosphere soil sample

AMB Express ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 2 ◽  
Author(s):  
Piyush Baindara ◽  
Santi M Mandal ◽  
Niharika Chawla ◽  
Pradip Kumar Singh ◽  
Anil Kumar Pinnaka ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Antimicrobial Peptides ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Subtilis Strain

Improved Production of 1-Deoxynojirmiycin via Mixed Cultivation of Bacillus subtilis and Fungi Isolated from Mulberry Rhizosphere

2012 ◽  
Vol 550-553 ◽  
pp. 1491-1495 ◽  
Author(s):  
Qian Huang ◽  
Qiang Li ◽  
Geng Sheng Ji ◽  
Ting Lu Fan ◽  
Xu Ding Gu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Soil Microorganisms ◽  
Rhizosphere Soil ◽  
Glucose Consumption ◽  
Dual Culture ◽  
Activity Assay ◽  
Rhizosphere Microorganisms ◽  
Mixed Cultivation ◽  
Dual Culture Assay

To help meet the challenge of 1-Deoxynojirmiycin (DNJ) manufacture from microbial fermentation, optimal condition for the fermentation has attracted substantial research interest. In the case, mix strategy was carried out to cultivate mulberry rhizosphere Bacillus Subtilis BJ-B121 (DNJ producing strain), Rhizopus spp.BJ-F13 and Aspergillus niger BJ-F8 according to the plate dual culture assay and inhibitory activity assay in vitro. We characterized mixed cultivation of Bacillus Subtilis BJ-B121 and mulberry rhizosphere microorganisms on the basis of biochemical data. Rhizosphere soil microorganisms may interact with each other and lead to improvement of DNJ production. Campared with mono culture, the glucose consumption and DNJ production were efficient. DNJ production was improved by 26-32% in mixed cultivation, 100 μg/mL and 308 μg/mL extra DNJ production were obtained. In addition, DNJ did not affect the growth of the mixed cells.

scholarly journals Degradación de oxalato por bacterias oxalotróficas asociadas a plantas del género Oxalis sp en regiones Andinas del departamento de Nariño, Colombia

2016 ◽  
Vol 18 (1) ◽  
pp. 69
Author(s):  
Roger David Castillo Arteaga ◽  
Edith Mariela Burbano-Rosero ◽  
Iván Darío Otero Ramirez ◽  
Pablo Fernández Izquierdo
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacterial Communities ◽  
Bacillus Amyloliquefaciens ◽  
Rhizosphere Soil ◽  
Acid Soils ◽  
Selective Medium ◽  
Daily Consumption ◽  
Promising Application ◽  
Bacillus Vallismortis

ResumenIntroducción: El ácido oxálico (H2C2O4) y las sales de oxalato son sustancias altamente oxidadas y consideradas tóxicas para algunos sistemas biológicos, incluido el humano, no obstante, pueden ser utilizadas como fuente de carbono y energía por algunas comunidades bacterianas, denominadas oxalotróficas, las cuales por su capacidad metabólica forman parte de la ruta biogeoquímica oxalato-carbonato (OCP, oxalate-carbonate pathway). Objetivo: Aislar y caracterizar bacterias oxalotróficas a partir de plantas del género Oxalis sp., de zonas alto-andina de Nariño- Colombia. Materiales y métodos: Se recolectaron muestras de suelo rizosférico de plantas oxalogénicas que fueron analizadas con parámetros fisicoquímicos y se utilizó un medio selectivo Schlegel para el aislamiento de bacterias oxalotróficas. Resultados: Las bacterias aisladas en medio selectivo Schlegel fueron caracterizadas bioquímicamente como: (Serratia fonticola, Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus vallismortis y Bacillus cereus). Estas especies fueron capaces de degradar oxalato e incrementar el pH producto de la degradación. Conclusión: Este tipo de bacterias pueden ser estudiadas en trabajos complementarios para evaluar su potencial como biofertilizantes y/o alternativas de bioremediación en suelos ácidos. El estudio a pesar de ser indicativo a nivel biológico, puede en un futuro y con base en mayores soportes en investigación, tornarse en una promisoria aplicación para reducir el oxalato de calcio en los alimentos de consumo diario que presentan un renglón de importancia agrícola en la región, potencialmente dañinos para la función renal.AbstractIntroduction: Oxalic acid (H2C2O4) and oxalate salts are highly oxidized substances, which are considered as toxic for some biological systems, including the human being; however, they can be used as a source of carbon and energy for some bacterial communities called oxalotrophic which are part of the so called oxalate-carbonate geochemistry pathway (OCP) due to its metabolic capacity. Objective: The aim of this research was to isolate and characterize oxalotrophic bacteria from plants of the genus Oxalis sp. in the high-andean zone of the department of Nariño, Colombia. Materials and methods: Samples of rhizosphere soil from oxalogenic plants were analyzed with physicochemical parameters and a Schlegel selective medium was used to isolate oxalotrophic bacteria. Results: The isolated bacteria through Schlegel selective medium were identified biochemically as: (Serratia fonticola, Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus vallismortis and Bacillus cereus). The identified species play an important role in the rhizosphere soil, principally for the capacity to increase the pH during oxalate consumption. Conclusion: This type of bacteria can be studied in additional studies to evaluate their potential as bio-fertilizers and/or bioremediation alternatives in acid soils. Despite that the study is indicative at a biological level, it can become a promising application, in the future and with greater support in research, to reduce the calcium oxalate in food of daily consumption which represent an agricultural important line in the region and are potentially harmful to kidney finction.

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