scholarly journals RHIZODEGRADATION OF PHENANTHRENE, ANTHRACENE AND PYRENE BY AUGMENTING BACILLUS CEREUS AND BACILLUS SUBTILIS STRAINS

2021 ◽  
Vol 9 (02) ◽  
pp. 89-96
Author(s):  
M. Poornachander Rao ◽  
◽  
Anitha Yerra ◽  
K. Satyaprasad ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Contaminated Soils ◽  
Rhizosphere Soil ◽  
Culture Method ◽  
Experimental Period ◽  
Bacterial Strains ◽  
Bacterial Populations ◽  
Metabolic Activities ◽  
Pot Culture

Rhizodegradation is one of the best methods for the effective removal of dangerous polycyclic aromatic hydrocarbons pollutants from soil. This is operative due to the high persistent, non-bioavailability nature of PAHs and combined, sequential reactions of bacteria present in rhizosphere of plants. We have conducted pot-culture method to study the degradation of three PAHs compounds namely phenanthrene, anthracene and pyrene in artificially contaminated soils of rhizosphere and non-rhizosphere soil treatments of blackgram(Vignamungo L.) that augmented by two potential PAHs degraders namely Bacillus cereus CPOU13 and Bacillus subtilis SPC14 isolated from naturally contaminated soils for 90days. HPLC studies revealed that degradation percentages of the three PAHs in treatments were more where selected strains augmented to the soil treatments over the non-augmented soils. The rhizosphere treatments that have augmented strains recorded more degradation percentages of phenanthrene, anthracene and pyrene over the rhizosphere treatments that were non-augmented. Pyrene, a high molecular weight PAHs degraded maximum to 96.24% in rhizosphere soil treatment that is augmented with the strains while moderate degradation of pyrene recorded in non-autoclaved soil treatments that contain natural microbial communities. The study of counting of bacterial populations during the experimental period revealed that the populations of the selected and other natural bacteria were gradually increased from the first day, reached maximum by 60days and became almost consistent in 90days in all the treatments. It was also observed that the populations of bacteria were high in rhizosphere treatments compared to the non-rhizosphere soil treatments. With these results it has been predicted that degradation of PAHs in rhizosphere soil treatments is closely associated with the increasing PAHs degrading bacterial populations of selected bacterial strains that may consume more quantity of PAHs for their metabolic activities in rhizosphere soils. Key words: Rhizodegradation, PAHs, HPLC, pot culture.

scholarly journals Enhanced Production of Biosurfactant from Bacillus subtilis Strain Al-Dhabi-130 under Solid-State Fermentation Using Date Molasses from Saudi Arabia for Bioremediation of Crude-Oil-Contaminated Soils

2020 ◽  
Vol 17 (22) ◽  
pp. 8446
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Galal Ali Esmail ◽  
Mariadhas Valan Arasu
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Crude Oil ◽  
Solid State Fermentation ◽  
Contaminated Soils ◽  
Quadratic Model ◽  
Subtilis Strain ◽  
Natural Environments ◽  
Bacterial Strains ◽  
Enhanced Production

Crude oil and its derivatives are the most important pollutants in natural environments. Bioremediation of crude oil using bacteria has emerged as a green cleanup approach in recent years. In this study, biosurfactant-producing Bacillus subtilis strain Al-Dhabi-130 was isolated from the marine soil sediment. This organism was cultured in solid-state fermentation using agro-residues to produce cost-effective biosurfactants for the bioremediation of crude-oil contaminated environments. Date molasses improved biosurfactant production and were used for further optimization studies. The traditional “one-variable-at-a-time approach”, “two-level full factorial designs”, and a response surface methodology were used to optimize the concentrations of date molasses and nutrient supplements for surfactant production. The optimum bioprocess conditions were 79.3% (v/w) moisture, 34 h incubation period, and 8.3% (v/v) glucose in date molasses. To validate the quadratic model, the production of biosurfactant was performed in triplicate experiments, with yields of 74 mg/g substrate. These findings support the applications of date molasses for the production of biosurfactants by B. subtilis strain Al-Dhabi-130. Analytical experiments revealed that the bacterial strain degraded various aromatic hydrocarbons and n-alkanes within two weeks of culture with 1% crude oil. The crude biosurfactant produced by the B. subtilis strain Al-Dhabi-130 desorbed 89% of applied crude oil from the soil sample. To conclude, biosurfactant-producing bacterial strains can increase emulsification of crude oil and support the degradation of crude oil.

scholarly journals Isolation of bacteria from diesel contaminated soil for diesel remediation

2019 ◽  
Vol 28 ◽  
pp. 33-41 ◽  
Author(s):  
OA Oyewole ◽  
SS Leh Togi Zobeashia ◽  
OE Oladoja ◽  
IO Musa ◽  
IT Terhemba
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Large Scale ◽  
Bacterial Species ◽  
Plastic Container ◽  
Hydrocarbon Concentration ◽  
Optimum Ph

This study is aimed at isolating bacterial species that inhabit diesel contaminated soil and also screened these isolates for the ability to be used for remediating diesel contaminated environment using their potential to degrade diesel as carbon and energy source. Top soil sample was collected from an ancient diesel-powered generator house in Minna, Nigeria, in a sterilized plastic container while diesel oil was obtained from local petrol bunk. Four bacterial isolates were isolated from the diesel contaminated soil sample and were screened for their ability to degrade diesel using mineral salt medium (MSM). The isolates with highest biodegradation potential were identified as Bacillus subtilis and Bacillus cereus. The optimum pH (5, 6, 7 and 8) and hydrocarbon concentration (1%, 2%, 5% and 10%) of the isolate was determined by spectrophotometry and the result revealed that the optimum pH for biodegradation of diesel by Bacillus subtilis and Bacillus cereus, was 7 (1.170) and 8 (1.745) respectively while the optimum hydrocarbon concentration degradation for both isolates was 5% (2.22) and 1% (2.37) respectively. The results of this study showed that these isolates were able to degrade diesel and can be useful for large scale bioremediation of diesel contaminated soils. J. bio-sci. 28: 33-41, 2020

scholarly journals Bacterial Tolerance to Heavy Metal Contents Present in Contaminated and Uncontaminated Soils

2016 ◽  
Vol 29 (2) ◽  
pp. 56-61
Author(s):  
FZ Tanu ◽  
S Hoque
Keyword(s):  
Heavy Metal ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Metal Tolerance ◽  
Micrococcus Luteus ◽  
Heavy Metal Tolerance ◽  
Subtilis Strain ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Metal Tolerant Bacteria

Present study dealt with identification of some heavy metal tolerant bacteria from contaminated industrial soils of Dhaka Export Processing Zone (DEPZ) at Savar, tannery area at Hazaribagh and uncontaminated agricultural soils of Dhamrai and Kushtia in Bangladesh and determination of their tolerance to chromium (Cr6+) and cadmium (Cd2+). A total of 15 isolates from four soil samples were provisionally identified as different species of Bacillus, Micrococcus and Pseudomonas based on their morphological, physiological, and biochemical characteristics. Among them eight colonies were separated based on high level of heavy metal tolerance and identified at molecular level by PCR technique and 16S rRNA gene sequencing as Micrococcus luteus strain P43 (E4), Bacillus pocheonensis strain TR2-6 (T6), Bacillus megaterium strain H2 (T8), Bacillus amyloliquefaciens strain SCSAAB0007 (D10), Bacillus cereus isolate PGBw4 (D11), Bacillus cereus strain ES-4a1 (K12), Bacillus subtilis strain 1320, (K13), and Bacillus subtilis strain DP14 (K14). The Maximum Tolerable Concentration (MTC) of bacterial strains to Cr6+ and Cd2+ ranged between 250-1250 ?g/ml and 30-150 ?g/ml, respectively in nutrient broth medium. From the metal tolerance investigation Bacillus was found as the most heavy metal tolerant to both Cr6+ and Cd2+ among the three genera. The identified heavy metal tolerant bacteria could be useful for the bioremediation of heavy metal contaminated environment.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 56-61

scholarly journals Biodegradation potential of bacterial isolates from dye wastewater at Marina, Sokoto metropolis

2020 ◽  
Vol 36 (2) ◽  
pp. 87-94
Author(s):  
M.A. Adegbite ◽  
K. Ibrahim ◽  
S.A. Yusif
Keyword(s):  
Waste Water ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacterial Species ◽  
Waste Water Treatment ◽  
Dye Wastewater ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Promising Tool ◽  
Degradation Ability

This study was carried out to determine the biodegradation potential of bacterial isolates from dye wastewater at Marina Area, Sokoto. Three (3) of the bacteria were identified; Bacillus subtilis, Luteimonasaestuarrii and Bacillus cereus and were used for further studies based on their degradation ability of green, red, yellow and blue dyes. Bacillus subtilis, Luteimonas aestuarii and Bacillus cereus have the potential to degrade all the four dyes used during the present investigation. However, efficacy of the various bacterial strains was found to vary at different concentration levels in degradation of a particular dye. Thus, by this study it can be concluded that Bacillus cereus, Bacillus subtilis and Luteimonas aestuarii can be used as a good microbial source for dye waste water treatment. The selected bacterial species represent a promising tool for application in biodegradation of dye waste water and the potential observed would allow for the application of the bacterial isolates for treatment of dye effluents before disposal. Keywords: effluents, isolates, biodegradation, dye and bacterial.

scholarly journals Degradación de oxalato por bacterias oxalotróficas asociadas a plantas del género Oxalis sp en regiones Andinas del departamento de Nariño, Colombia

2016 ◽  
Vol 18 (1) ◽  
pp. 69
Author(s):  
Roger David Castillo Arteaga ◽  
Edith Mariela Burbano-Rosero ◽  
Iván Darío Otero Ramirez ◽  
Pablo Fernández Izquierdo
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacterial Communities ◽  
Bacillus Amyloliquefaciens ◽  
Rhizosphere Soil ◽  
Acid Soils ◽  
Selective Medium ◽  
Daily Consumption ◽  
Promising Application ◽  
Bacillus Vallismortis

ResumenIntroducción: El ácido oxálico (H2C2O4) y las sales de oxalato son sustancias altamente oxidadas y consideradas tóxicas para algunos sistemas biológicos, incluido el humano, no obstante, pueden ser utilizadas como fuente de carbono y energía por algunas comunidades bacterianas, denominadas oxalotróficas, las cuales por su capacidad metabólica forman parte de la ruta biogeoquímica oxalato-carbonato (OCP, oxalate-carbonate pathway). Objetivo: Aislar y caracterizar bacterias oxalotróficas a partir de plantas del género Oxalis sp., de zonas alto-andina de Nariño- Colombia. Materiales y métodos: Se recolectaron muestras de suelo rizosférico de plantas oxalogénicas que fueron analizadas con parámetros fisicoquímicos y se utilizó un medio selectivo Schlegel para el aislamiento de bacterias oxalotróficas. Resultados: Las bacterias aisladas en medio selectivo Schlegel fueron caracterizadas bioquímicamente como: (Serratia fonticola, Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus vallismortis y Bacillus cereus). Estas especies fueron capaces de degradar oxalato e incrementar el pH producto de la degradación. Conclusión: Este tipo de bacterias pueden ser estudiadas en trabajos complementarios para evaluar su potencial como biofertilizantes y/o alternativas de bioremediación en suelos ácidos. El estudio a pesar de ser indicativo a nivel biológico, puede en un futuro y con base en mayores soportes en investigación, tornarse en una promisoria aplicación para reducir el oxalato de calcio en los alimentos de consumo diario que presentan un renglón de importancia agrícola en la región, potencialmente dañinos para la función renal.AbstractIntroduction: Oxalic acid (H2C2O4) and oxalate salts are highly oxidized substances, which are considered as toxic for some biological systems, including the human being; however, they can be used as a source of carbon and energy for some bacterial communities called oxalotrophic which are part of the so called oxalate-carbonate geochemistry pathway (OCP) due to its metabolic capacity. Objective: The aim of this research was to isolate and characterize oxalotrophic bacteria from plants of the genus Oxalis sp. in the high-andean zone of the department of Nariño, Colombia. Materials and methods: Samples of rhizosphere soil from oxalogenic plants were analyzed with physicochemical parameters and a Schlegel selective medium was used to isolate oxalotrophic bacteria. Results: The isolated bacteria through Schlegel selective medium were identified biochemically as: (Serratia fonticola, Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus vallismortis and Bacillus cereus). The identified species play an important role in the rhizosphere soil, principally for the capacity to increase the pH during oxalate consumption. Conclusion: This type of bacteria can be studied in additional studies to evaluate their potential as bio-fertilizers and/or bioremediation alternatives in acid soils. Despite that the study is indicative at a biological level, it can become a promising application, in the future and with greater support in research, to reduce the calcium oxalate in food of daily consumption which represent an agricultural important line in the region and are potentially harmful to kidney finction.

scholarly journals Intake and digestibility by lambs of a diet of tropical grass and Hyparrhenia rufa hays with a probiotic containing Bacillus licheniformis and Bacillus subtilis

1969 ◽  
Vol 98 (2) ◽  
pp. 147-168
Author(s):  
Abner A. Rodríguez ◽  
Enrique M. Martínez ◽  
Luis C. Solórzano ◽  
Paul F. Randel
Keyword(s):  
Bacillus Subtilis ◽  
Animal Health ◽  
Residual Effect ◽  
Live Weight ◽  
Basal Diet ◽  
Experimental Period ◽  
Neutral Detergent Fiber ◽  
Residual Effects ◽  
Bacterial Strains ◽  
Hyparrhenia Rufa

An experiment consisting of two periods (P1 and P2) was conducted to determine the effect of adding a probiotic of bacterial strains of Bacillus subtilis and B. licheniformis to a basal diet of low quality grass hays on voluntary consumption (VC) and digestibility. Ten Creole lambs were randomly assigned to one of two treatments: control (without additive) or probiotic (with additive In diet). The basal diet consisted of a dally forage offering equal to 4% of live weight (LW) on a dry matter (DM) basis. The forage offered was 50% native grass hay [71.7% neutral detergent fiber (NDF), 4.9% crude protein (CP) in P1; and 71.2% NDF, 5.4% CP in P2], and 50% of Hyparrhenia rufa hay (78.8% NDF, 3.5% CP in P1; and 75.6% NDF, 5.5% CP in P2). The lambs were supplemented with 225 g of commercial concentrate (CC) daily. The additive was mixed with the CC to supply 1.33 X ICcfu/head daily during the 49 days of P1. From day 50 to 84 (P2), the probiotic addition was suspended to determine possible residual effects. The lambs were weighed weekly. The VC and digestibility of DM, NDF and CP were determined from day 42 to 49 in P1 and from day 77 to 84 in P2. The variables related to parasitism and anemia: fecal egg count (FEC), FAMACHA® index score and packed cell volume (PCV) were determined every 21 days. Data from each experimental period were analyzed according to a completely randomized design with five replicas per treatment. During P1, the daily LW gain of the lambs was 23 vs. 20 g for control and probiotic treatments, respectively. The dietary addition of probiotic increased (p < 0.05) total DM intake (445 vs. 484 g/d), DM intake as a percentage of LW (2.04 vs. 2.37) and forage DM as a percentage of total DM intake (54.77 vs. 59.42). The digestibility coefficients of DM (59.98 vs. 62.62%) and CP (59.35 vs. 61.76%) did not differ between treatments, but there was a tendency (p = 0.09) to improve NDF digestibility (58.71 vs. 62.48%) with probiotic addition. The FEC observed in the control and probiotic groups were 820 vs. 1,380 eggs/g initially and increased more in the control (p < 0.05) to 2,390 vs. 2,780 eggs/g at day 21, then decreased less in this group to 1,830 vs. 1,480 eggs/g at day 42. The PCV values changed between days 0 and 42 from 24.4 to 17.9% in the control and from 20.6 to 22.6% in the probiotic group, but without significant effects (p > 0.05). The anemia level according to FAMACHA® score differed little between treatments and did not exceed a maximum of 2.6. During P2, the LW gain of the lambs of both control and previously probiotic treated groups was 48 g/d. Total DM intake was 587 vs. 562 g/d and digestibilities were: DM (58.46 vs. 57.59%), NDF (57.50 vs. 56.85%) and CP (60.78 vs. 62.11%) without significant differences (p > 0.05). The FEC decreased progressively to respective final values at 84 days of 1,230 vs. 440 eggs/g, whereas the PCV increased to 23.4 vs. 25.1% at day 84 without significant differences. The maximum FAMACHA® score was 2.8 vs. 2.2 in the two respective treatments. In summary, the addition of the probiotic in the diet improved VC and tended to increase NDF digestibility, but did not affect growth, even though there were signs of animal health benefiting. After suspending the use of the additive, no residual effect on the variables evaluated was observed.

scholarly journals Optimization and Molecular Characterization of Lipase Producing Bacillus subtilis Strain Rcps3 and Bacillus fumarioli Strain Rcps4 from Oil-contaminated Soils of Warangal

2020 ◽  
Vol 14 (3) ◽  
pp. 2063-2074
Author(s):  
Ramya Chouhan ◽  
Suresh Lapaka ◽  
Nagaraju Alpula ◽  
Srinivas Podeti
Keyword(s):  
Bacillus Subtilis ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Lipase Production ◽  
Subtilis Strain ◽  
Bacterial Strains ◽  
Almost All ◽  
Bacterial Sources ◽  
Hydrolysis Of

Microbial sources are regularly used as reliable biocatalysts sources which are often used in the process and production industry. Demands for such organisms with greater capacity of intended enzyme production are on the rise. Lipase is important enzyme used in the biotechnological process of hydrolysis of fats in almost all the relevant industries We have utilized the local oil-contaminated soil resources to search for efficacious bacterial strains that have excellent lipase activity. We were successful in identifying two such bacterial sources, namely, Bacillus subtilis strain RCPS3 and Bacillus fumarioli strain RCPS4, responsible for lipase production from oil effluent contaminated soil of Telangana. This is the first report of these two strains from this part of India that are involved in lipase production. The strains were isolated, optimized, and purified using standard microbiology protocols and were characterized at the molecular level using the biomarker 16s ribosomal RNA genes of the strains. The identified and isolated bacterial strains were confirmed as Bacillus subtilis strain RCPS3, and Bacillus fumarioli strain RCPS4 through molecular and computational characterization.

scholarly journals Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 912
Author(s):  
Shuming Liu ◽  
Hongmei Liu ◽  
Rui Chen ◽  
Yong Ma ◽  
Bo Yang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Translocation Factor ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Pgp Traits ◽  
Cd Tolerance ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Cd Translocation

Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.

scholarly journals Intracellular localization of the mycobacterial stressosome complex

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Malavika Ramesh ◽  
Ram Gopal Nitharwal ◽  
Phani Rama Krishna Behra ◽  
B. M. Fredrik Pettersson ◽  
Santanu Dasgupta ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fluorescence Microscopy ◽  
Bacillus Cereus ◽  
Intracellular Localization ◽  
Sigma Factors ◽  
Alternative Sigma Factors ◽  
Expression Of Genes ◽  
Molecular Machinery ◽  
Stress Signals ◽  
Activation Pathways

AbstractMicroorganisms survive stresses by alternating the expression of genes suitable for surviving the immediate and present danger and eventually adapt to new conditions. Many bacteria have evolved a multiprotein "molecular machinery" designated the "Stressosome" that integrates different stress signals and activates alternative sigma factors for appropriate downstream responses. We and others have identified orthologs of some of the Bacillus subtilis stressosome components, RsbR, RsbS, RsbT and RsbUVW in several mycobacteria and we have previously reported mutual interactions among the stressosome components RsbR, RsbS, RsbT and RsbUVW from Mycobacterium marinum. Here we provide evidence that "STAS" domains of both RsbR and RsbS are important for establishing the interaction and thus critical for stressosome assembly. Fluorescence microscopy further suggested co-localization of RsbR and RsbS in multiprotein complexes visible as co-localized fluorescent foci distributed at scattered locations in the M. marinum cytoplasm; the number, intensity and distribution of such foci changed in cells under stressed conditions. Finally, we provide bioinformatics data that 17 (of 244) mycobacteria, which lack the RsbRST genes, carry homologs of Bacillus cereus genes rsbK and rsbM indicating the existence of alternative σF activation pathways among mycobacteria.

Sign in / Sign up

Export Citation Format

Share Document