Genome-wide identification, annotation and characterization of novel thermostable cytochrome P450 monooxygenases from the thermophilic biomass-degrading fungi Thielavia terrestris and Myceliophthora thermophila

2014 ◽  
Vol 36 (3) ◽  
pp. 321-333 ◽  
Author(s):  
Khajamohiddin Syed ◽  
Karabo Shale ◽  
K. H. M. Nazmul Hussain Nazir ◽  
Nada Krasevec ◽  
Samson Sitheni Mashele ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Monooxygenases ◽  
Myceliophthora Thermophila ◽  
Thielavia Terrestris ◽  
P450 Monooxygenases ◽  
Genome Wide

Characterization of cytochrome P450-monooxygenases of Chinese hamsters with respect to aflatoxin B1 activation

Toxicology ◽  
1994 ◽  
Vol 93 (2-3) ◽  
pp. 165-173 ◽  
Author(s):  
Morio Fukuhara ◽  
Eric Antignac ◽  
Naomi Fukusen ◽  
Kazue Kato ◽  
Masanobu Kimura
Keyword(s):  
Cytochrome P450 ◽  
Aflatoxin B1 ◽  
Cytochrome P450 Monooxygenases ◽  
P450 Monooxygenases ◽  
Chinese Hamsters

scholarly journals Construction and Application of a Functional Library of Cytochrome P450 Monooxygenases from the Filamentous Fungus Aspergillus oryzae

2011 ◽  
Vol 77 (9) ◽  
pp. 3147-3150 ◽  
Author(s):  
K. H. M. Nazmul Hussain Nazir ◽  
Hirofumi Ichinose ◽  
Hiroyuki Wariishi
Keyword(s):  
Cytochrome P450 ◽  
Aspergillus Oryzae ◽  
Cytochrome P450 Monooxygenases ◽  
Functional Screening ◽  
Content Type ◽  
P450 Monooxygenases ◽  
Rapid Characterization ◽  
Catalytic Functions ◽  
First Time

ABSTRACTA functional library of cytochrome P450 monooxygenases fromAspergillus oryzae(AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase inSaccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs.

Cloning, Expression and Characterization of a Monooxygenase P450BM3 from Bacillus megaterium ALA2

2012 ◽  
Vol 518-523 ◽  
pp. 5533-5538
Author(s):  
Ting Wang ◽  
Liang Liang Wang ◽  
Xun Li
Keyword(s):  
Escherichia Coli ◽  
Cytochrome P450 ◽  
Linoleic Acid ◽  
Bacillus Megaterium ◽  
Cytochrome P450 Monooxygenases ◽  
P450 Monooxygenases ◽  
Nadph Oxidation ◽  
First Time ◽  
Optimal Ph

Cytochrome P450 monooxygenases are enzymes which are capable of oxidising saturated and unsaturated substrates. P450BM3 from Bacillus megaterium is one of this family. For the first time, the cyp gene for coding P450BM3 from B. megaterium ALA2 has been cloned and expressed in Escherichia coli. The recombinant enzyme is 120 kDa, containing 1049 aa. The highest activity of purified enzyme is 14.8 U/mg towards palmitic acid by monitoring the NADPH oxidation. The optimal pH and temperature were 9.0 and 40°C. The enzyme has higher activity towards linoleic acid, and 2-Methyl-7-octadecene can also be catalyzed which is a precursor of displar.

scholarly journals Biocatalytic Conversion of Avermectin to 4"-Oxo-Avermectin: Characterization of Biocatalytically Active Bacterial Strains and of Cytochrome P450 Monooxygenase Enzymes and Their Genes

2005 ◽  
Vol 71 (11) ◽  
pp. 6968-6976 ◽  
Author(s):  
Volker Jungmann ◽  
István Molnár ◽  
Philip E. Hammer ◽  
D. Steven Hill ◽  
Ross Zirkle ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cytochrome P450 ◽  
Recombinant Proteins ◽  
Oxidation Reaction ◽  
Cytochrome P450 Monooxygenases ◽  
Enzyme Kinetic ◽  
Bacterial Strains ◽  
P450 Monooxygenase ◽  
P450 Monooxygenases

ABSTRACT 4"-Oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin. Seventeen biocatalytically active Streptomyces strains with the ability to oxidize avermectin to 4"-oxo-avermectin in a regioselective manner have been discovered in a screen of 3,334 microorganisms. The enzymes responsible for this oxidation reaction in these biocatalytically active strains were found to be cytochrome P450 monooxygenases (CYPs) and were termed Ema1 to Ema17. The genes for Ema1 to Ema17 have been cloned, sequenced, and compared to reveal a new subfamily of CYPs. Ema1 to Ema16 have been overexpressed in Escherichia coli and purified as His-tagged recombinant proteins, and their basic enzyme kinetic parameters have been determined.

Functional characterization of cytochrome P450 monooxygenases in the cereal head blight fungusFusarium graminearum

2017 ◽  
Vol 19 (5) ◽  
pp. 2053-2067 ◽  
Author(s):  
Ji Young Shin ◽  
Duc-Cuong Bui ◽  
Yoonji Lee ◽  
Hyejin Nam ◽  
Soyun Jung ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Functional Characterization ◽  
Cytochrome P450 Monooxygenases ◽  
Head Blight ◽  
P450 Monooxygenases

scholarly journals Genome-Wide Annotation and Comparative Analysis of Cytochrome P450 Monooxygenases in Basidiomycete Biotrophic Plant Pathogens

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142100 ◽  
Author(s):  
Lehlohonolo Benedict Qhanya ◽  
Godfrey Matowane ◽  
Wanping Chen ◽  
Yuxin Sun ◽  
Elizabeth Mpholoseng Letsimo ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Comparative Analysis ◽  
Plant Pathogens ◽  
Cytochrome P450 Monooxygenases ◽  
P450 Monooxygenases ◽  
Genome Wide

scholarly journals Distribution and Diversity of Cytochrome P450 Monooxygenases in the Fungal Class Tremellomycetes

2019 ◽  
Vol 20 (12) ◽  
pp. 2889 ◽  
Author(s):  
Olufunmilayo Olukemi Akapo ◽  
Tiara Padayachee ◽  
Wanping Chen ◽  
Abidemi Paul Kappo ◽  
Jae-Hyuk Yu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Monooxygenases ◽  
The Novel ◽  
Human Pathogens ◽  
Fungal Cell ◽  
P450 Monooxygenase ◽  
Over Expression ◽  
P450 Monooxygenases ◽  
Genome Wide ◽  
Novel Drug

Tremellomycetes, a fungal class in the subphylum Agaricomycotina, contain well-known opportunistic and emerging human pathogens. The azole drug fluconazole, used in the treatment of diseases caused by some species of Tremellomycetes, inhibits cytochrome P450 monooxygenase CYP51, an enzyme that converts lanosterol into an essential component of the fungal cell membrane ergosterol. Studies indicate that mutations and over-expression of CYP51 in species of Tremellomycetes are one of the reasons for fluconazole resistance. Moreover, the novel drug, VT-1129, that is in the pipeline is reported to exert its effect by binding and inhibiting CYP51. Despite the importance of CYPs, the CYP repertoire in species of Tremellomycetes has not been reported to date. This study intends to address this research gap. Comprehensive genome-wide CYP analysis revealed the presence of 203 CYPs (excluding 16 pseudo-CYPs) in 23 species of Tremellomycetes that can be grouped into 38 CYP families and 72 CYP subfamilies. Twenty-three CYP families are new and three CYP families (CYP5139, CYP51 and CYP61) were conserved across 23 species of Tremellomycetes. Pathogenic cryptococcal species have 50% fewer CYP genes than non-pathogenic species. The results of this study will serve as reference for future annotation and characterization of CYPs in species of Tremellomycetes.

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