Effective detection of Lily Symptomless Virus using the reverse transcription loop–mediated isothermal amplification method

2019 ◽  
Vol 48 (4) ◽  
pp. 373-374 ◽  
Author(s):  
Xiao Zhao ◽  
Yanan Du ◽  
Yongchun Zhang ◽  
Jieling Liang ◽  
Youming Cai ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Isothermal Amplification ◽  
Lily Symptomless Virus ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method

scholarly journals Variability of Potato virus Y in Tomato Crops in Poland and Development of a Reverse-Transcription Loop-Mediated Isothermal Amplification Method for Virus Detection

2015 ◽  
Vol 105 (9) ◽  
pp. 1270-1276 ◽  
Author(s):  
Beata Hasiów-Jaroszewska ◽  
Joanna Stachecka ◽  
Julia Minicka ◽  
Mateusz Sowiński ◽  
Natasza Borodynko
Keyword(s):  
Potato Virus ◽  
Reverse Transcription ◽  
Potato Virus Y ◽  
Protein Gene ◽  
Virus Detection ◽  
Isothermal Amplification ◽  
Chain Reaction ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Polymerase Chain

A collection of 147 Potato virus Y (PVY) isolates from tomato, originating from several commercial fields and greenhouses in different regions of Poland, was tested for the presence of PVY by reverse-transcription polymerase chain reaction. However, in some cases, the results obtained were ambiguous. Therefore, a sensitive reverse-transcription loop-mediated isothermal amplification method was developed for rapid detection of PVY isolates. Phylogenetic and recombination analyses were performed based on sequences of the coat protein gene. In comparison with results obtained in 2008, the presence of other strains besides PVYNWi-P was confirmed. A novel recombinant between PVYNTN and PVYNWi-P strains was detected. Our results indicate an increasing distribution and variability of the PVY population on tomato in Poland.

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