scholarly journals A Mismatch-Tolerant Reverse Transcription Loop-Mediated Isothermal Amplification Method and Its Application on Simultaneous Detection of All Four Serotype of Dengue Viruses

2019 ◽  
Vol 10 ◽  
Author(s):  
Yi Zhou ◽  
Zhenzhou Wan ◽  
Shuting Yang ◽  
Yingxue Li ◽  
Min Li ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Isothermal Amplification ◽  
Dengue Viruses ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method

scholarly journals Variability of Potato virus Y in Tomato Crops in Poland and Development of a Reverse-Transcription Loop-Mediated Isothermal Amplification Method for Virus Detection

2015 ◽  
Vol 105 (9) ◽  
pp. 1270-1276 ◽  
Author(s):  
Beata Hasiów-Jaroszewska ◽  
Joanna Stachecka ◽  
Julia Minicka ◽  
Mateusz Sowiński ◽  
Natasza Borodynko
Keyword(s):  
Potato Virus ◽  
Reverse Transcription ◽  
Potato Virus Y ◽  
Protein Gene ◽  
Virus Detection ◽  
Isothermal Amplification ◽  
Chain Reaction ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Polymerase Chain

A collection of 147 Potato virus Y (PVY) isolates from tomato, originating from several commercial fields and greenhouses in different regions of Poland, was tested for the presence of PVY by reverse-transcription polymerase chain reaction. However, in some cases, the results obtained were ambiguous. Therefore, a sensitive reverse-transcription loop-mediated isothermal amplification method was developed for rapid detection of PVY isolates. Phylogenetic and recombination analyses were performed based on sequences of the coat protein gene. In comparison with results obtained in 2008, the presence of other strains besides PVYNWi-P was confirmed. A novel recombinant between PVYNTN and PVYNWi-P strains was detected. Our results indicate an increasing distribution and variability of the PVY population on tomato in Poland.

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