scholarly journals Different response of perennial ryegrass—Epichloë endophyte symbiota to the elevated concentration of heavy metals in soil

Author(s):  
Grzegorz Żurek ◽  
Barbara Wiewióra ◽  
Krystyna Rybka ◽  
Kamil Prokopiuk

Abstract The phenomenon of plant mutualistic symbiosis with microbes may have a positive effect on the improvement of plant tolerance to environmental stresses as well as on the ability of plants to accumulate heavy metal (HM) ions from soil. The influence of Epichloë fungal endophyte (Ascomycota, Clavicipitaceae) on perennial ryegrass (Lolium perenne L.) plants grown in the presence of elevated concentrations of HM ions (Cd2+, Pb2+, and Cu2+) in soil was studied. The presence of Epichloë in the host grass tissues resulted in different accumulation of HM ions in the aboveground parts of the plants. In some cases, endophyte infection positively affected ryegrass ability to accumulate HM ions from soil. In plants with (E +) and without (E −) endophytes, the hormesis effect was induced by the elevated concentration of Cu2+ ions, resulting in better growth and photosynthesis, as examined by measurements of Chl a fluorescence. The obtained results indicate that based on the laboratory evaluation of the efficiency of HM accumulation, we were able to choose the best associations of perennial ryegrass with endophytes for HM phytoremediation.

2021 ◽  
Author(s):  
Grzegorz Żurek ◽  
Barbara Wiewióra ◽  
Krystyna Rybka ◽  
Kamil Prokopiuk

Abstract The phenomenon of plant mutualistic symbiosis with microbes may have a positive effect on the improvement of plant tolerance to environmental stresses as well as on the ability of plants to accumulate heavy metal (HM) ions from soil. The influence of Epichloë fungal endophyte (Ascomycota, Clavicipitaceae) on perennial ryegrass (Lolium perenne L.) plants grown in the presence of elevated concentrations of HM ions (Cd2+, Pb2+ and Cu2+) in soil was studied. The presence of Epichloë in the host grass tissues resulted in different accumulation of HM ions in the aboveground parts of the plants. In some cases endophyte infection positively affected ryegrass ability to accumulate HM ions from soil. In plants with (E+) and without (E-) endophytes the hormesis effect was induced by the elevated concentration of Cu2+ ions, resulting in better growth and photosynthesis, as examined by measurements of Chl a fluorescence. The obtained results indicate that based on the laboratory evaluation of the efficiency of HM accumulation, we were able to choose the best associations of perennial ryegrass with endophytes for HM phytoremediation.


2020 ◽  
Author(s):  
Grzegorz Żurek ◽  
Barbara Wiewióra ◽  
Krystyna Rybka ◽  
Kamil Prokopiuk

Abstract Background: The phenomenon of plant mutualistic symbiosis with microbes may have a positive effect on the improvement of plant tolerance to environmental stresses. The influence of fungal endophyte of the Epichloë sp. (Clavicipitaceae) on perennial ryegrass (Lolium perenne L.) plants grown in presence of elevated concentration of heavy metal (HM) ions (Cd2+, Pb2+ and Cu2+) in soil was studied.Results: The presence of Epichloë in the host grass tissues resulted in increased accumulation of HM ions in aerial parts of plants and was dependent on host genotypes related to host plant origin. In plants with (E+) and without (E-) endophytes the hormesis effect was induced by the elevated concentration of Cu2+ ions, resulting in better growth and photosynthesis, as examined by measurements of Chl a fluorescence. The obtained results indicate that based on the laboratory evaluation of the efficiency of the symbiosis, we were able to choose the best associations of perennial ryegrass with endophytes for HM phytoremediation.Conclusions: The presence of Epichloë endophytes positively affected ryegrass ability to accumulate HM ions and this accumulation was associated with the origin of Epichloë-ryegrass symbionts.


2020 ◽  
Author(s):  
Grzegorz Żurek ◽  
Barbara Wiewióra ◽  
Krystyna Rybka ◽  
Kamil Prokopiuk

Abstract Background: The phenomenon of plant mutualistic symbiosis with microbes may have a positive effect on improvement of plant tolerance to environmental stresses. The influence of fungal endophyte of the Epichloë sp. (Clavicipitaceae) on perennial ryegrass (Lolium perenne L.) plants grown in presence of elevated concentration of heavy metal (HM) ions: Cd2+, Pb2+ and Cu2+, in soil was studied. Results: The presence of Epichloë in the host grass tissues resulted in increased accumulation of HM ions in aerial parts of plants and was dependent on host genotypes related to host plant origin. In plants with (E+) and without (E-) endophytes the hormesis effect was induced by elevated concentration of Cu2+ ions, resulting in better growth and photosynthesis, as examined by measurements of Chl a fluorescence. The obtained results indicate that based on the laboratory evaluation of the efficiency of the symbiosis, we were able to choose the best associations of perennial ryegrass with endophytes for HM phytoremediation. Conclusions: The presence of Epichloë endophytes positively affected ryegrass ability to accumulate HM ions and this accumulation was associated with the origin of Epichloë-ryegrass symbionts.


2020 ◽  
Author(s):  
Grzegorz Żurek ◽  
Barbara Wiewióra ◽  
Krystyna Rybka ◽  
Kamil Prokopiuk

Abstract Background: The phenomenon of plant mutualistic symbiosis with microbes may have apositive effect on improvement of plant tolerance to environmental stresses. The influence of fungal endophyte of the Epichloë sp. ( Clavicipitaceae ) on perennial ryegrass ( Lolium perenne L.) plants grown in presence of heavy metal (HM) ions, Cd, Pb and Cu, in soil was studied. Results : The presence of Epichloë in the host grass tissues resulted in increase of the chlorophyll content, efficiency of photosystem II and accumulation of HM ions in aerial parts of plants and was dependent on host genotypes related to host plant origin. In both, E+ and E- plants the hormesis effect was induced by elevated concentration of Cu ions, resulting in better growth and photosynthesis, as examined by measurements of Chl a fluorescence. In case of an application of Epichloë -colonized grasses for HM phytoremediation, the choice of most effective host-endophyte interactions should be based on laboratory evaluation of the efficiency of the symbiosis. Conclusions: The presence of Epichloë endophytes positively affected ryegrass ability to accumulate HM ions and this accumulation was related to origin of Epichloë -ryegrass symbionts.


2016 ◽  
Vol 141 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Liang Cheng ◽  
Ning Zhang ◽  
Bingru Huang

The accumulation of 1-aminocyclopropane-1-carboxylate (ACC), which is a precursor for ethylene production, in plant roots exposed to salinity stress can be detrimental to plant growth. The objectives of this study were to determine whether inoculating roots with bacteria containing deaminase enzymes that break down ACC (ACC-deaminase) could improve plant tolerance to salinity in perennial ryegrass (Lolium perenne) and to examine growth and physiological factors, as well as nutrition status of plants affected by the ACC-deaminase bacteria inoculation under salinity stress. Plants of perennial ryegrass (cv. Pangea) were inoculated with either Burkholderia phytofirmans PsJN or Burkholderia gladioli RU1 and irrigated with either fresh water (control) or a 250 mm NaCl solution to induce salinity stress. The bacterium-inoculated plants had less ACC content in shoots and roots under both nonstressed and salinity conditions. Salinity stress inhibited root and shoot growth, but the bacterium-inoculated plants exhibited higher visual turf quality (TQ), tiller number, root biomass, shoot biomass, leaf water content, and photochemical efficiency, as well as lower cellular electrolyte leakage (EL) under salinity stress. Plants inoculated with bacteria had lower sodium content and higher potassium to sodium ratios in shoots under salinity stress. Shoot and root nitrogen content and shoot potassium content increased, whereas shoot and root calcium, magnesium, iron, and aluminum content all decreased due to bacterial inoculation under salinity treatment. ACC-deaminase bacteria inoculation of roots was effective in improving salinity tolerance of perennial ryegrass and could be incorporated into turfgrass maintenance programs in salt-affected soils.


2020 ◽  
Vol 11 ◽  
Author(s):  
Flavia Pilar Forte ◽  
Jan Schmid ◽  
Paul P. Dijkwel ◽  
Istvan Nagy ◽  
David E. Hume ◽  
...  

2015 ◽  
Vol 35 (8) ◽  
Author(s):  
Marty J. Faville ◽  
Lyn Briggs ◽  
Mingshu Cao ◽  
Albert Koulman ◽  
M. Z. Zulfi Jahufer ◽  
...  

Author(s):  
Eline van Zijll de Jong ◽  
Nathaniel R. Bannan ◽  
Jacqueline Batley ◽  
Kathryn M. Guthridge ◽  
German C. Spangenberg ◽  
...  

2014 ◽  
Vol 53 (05) ◽  
pp. 406-415 ◽  
Author(s):  
I. C. M. Flinsenberg ◽  
E. J. van Loenen ◽  
R. P. G. Cuppen ◽  
R. J. E. Rajae-Joordens ◽  
E. M. L. Daemen

SummaryIntroduction: This article is part of the Focus Theme of Methods of Information in Medicine on “Pervasive Intelligent Technologies for Health”.Background: This paper addresses the evaluation with hospital staff of an in-patient environment that supports patients, family, nursing staff and medical specialists during the recovery process of neurology patients and especially patients recovering from a stroke. We describe the methods that were used to evaluate the Adaptive Daily Rhythm Atmospheres (ADRA), Artificial Skylight (AS) and Adaptive Stimulus Dosage (ASD) concepts.Objectives: The goal of this evaluation was to gather qualitative and quantitative feedback from hospital staff about the usefulness, the usability and desirability of the Adaptive Daily Rhythm Atmospheres (ADRA), Artificial Skylight (AS) and Adaptive Stimulus Dosage (ASD) concepts that were implemented as different phases of a novel healing patient room. This paper reports the effects of these concepts with regard to 1) the healing process of the patient and 2) the workflow of the staff. These results are part of a larger R&D project and provide the initial feedback in an iterative user-centered design methodology.Methods: After signing informed consents, the group of participants was taken to the laboratory environment where they were introduced to the Adaptive Healing Environment Patient Room and where they could also experience the room. Then, the participants were seated next to the patient bed so they had a similar viewing angle as the patients. The participants received a booklet with questionnaires. The items on this questionnaire addressed the influence on the healing process (i.e., the possible effect the concept/phase has on the healing process of the patient, meaning faster recovery, better sleep and enhanced well-being) and influence on the workflow (i.e., the possible effect of such a concept/ phase on the working activities of the staff in the ward). We presented every concept (AS and ASD) and all the phases of ADRA. After every presentation of the concept or phase of the ADRA system the participants rated the concept or phase anonymously on a 7-point Likert scale. In addition to rating the phase in the therefore designed booklets, they were also asked to motivate their ratings in writing. Subsequently, a focus group discussion took place. During the discussion the two note takers wrote down all the comments. Afterwards the quantitative results were analyzed with the non-parametric Kruskal-Wallis test. Significant effects were further analyzed in a post-hoc Mann-Whitney test.Results: The results show that hospital staff expects a positive effect on the healing process of the patient for the Artificial Skylight, the Adaptable Stimulus Dosage concept and the different ADRA phases that provide a clear daily rhythm structure during the day. In fact the staff members from different healthcare institutions and with different professional roles agreed on most aspects. In addition, the staff also expected a positive effect for almost all phases on the efficiency of the clinical workflow, also for the AS and ASD concepts. This is a very promising result as the phases were designed primarily with the healing effect of the patient in mind.Conclusions: The hospital staff evaluation in the laboratory setting gave us an indication of the likely impact of the Adaptive Healing Environment Patient Room on the healing progress of patients. Furthermore, this laboratory evaluation of the concepts was an important step that enabled to improve the shortcomings of the current concept before starting clinical trials. In addition, we generated feedback from different departments from different institutions, which suggest that they all see similar added values for the patient room


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