Production of an epigenetic mutant population of Populus nigra: DNA methylation and phenotype analyses

2020 ◽  
Author(s):  
Shanchen Zhong ◽  
Yanan Gao ◽  
Junqian Lu ◽  
Changjun Ding ◽  
Xiaohua Su ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Populus Nigra ◽  
Mutant Population

scholarly journals Genome-wide analysis of day/night DNA methylation differences in Populus nigra

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0190299 ◽  
Author(s):  
Chang-Jun Ding ◽  
Li-Xiong Liang ◽  
Shu Diao ◽  
Xiao-Hua Su ◽  
Bing-Yu Zhang
Keyword(s):  
Dna Methylation ◽  
Populus Nigra ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Assessment of Epigenetic and Phenotypic Variation in Populus nigra Regenerated via Sequential Regeneration

2021 ◽  
Vol 12 ◽  
Author(s):  
Weixi Zhang ◽  
Yanbo Wang ◽  
Shu Diao ◽  
Shanchen Zhong ◽  
Shu Wu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Phenotypic Variation ◽  
Populus Nigra ◽  
Utilization Efficiency ◽  
Plant Genome ◽  
Physiological Traits ◽  
Somatic Variation ◽  
Regenerated Plants ◽  
Significant Difference ◽  
Almost All

Somatic variation has been demonstrated in tissue culture regenerated plants of many species. In the genus Populus, phenotypic variation caused by changes in 5-methylcytosine within the plant genome have been reported. To date, the phenotypic and epigenetic stability of plants regenerated from sequential regeneration has not been tested in trees. In this study, we detected DNA methylation of CCGG sites in regenerated plants of five generations in Populus nigra using methylation-sensitive amplified polymorphisms, and evaluated their growth performance and physiological traits. About 10.86–26.80% of CCGG sites in the regenerated plant genome were demethylated and 5.50–8.45% were methylated, resulting in significantly lower DNA methylation levels among all regenerated plants than among donor plants. We detected a significant difference in methylation levels between first regeneration regenerated plants (G1) and those of the other four generations (G2–G5); there were no significant differences among the four later generations. Therefore, the dramatic decrease in DNA methylation levels occurred only in the first and second poplar regenerations; levels then stabilized later in the regeneration process, indicating that two regeneration events were sufficient to change the methylation statuses of almost all CCGG sites sensitive to regeneration. Differences in growth and physiological traits were observed between regenerated plants and donor plants, but were significant only among plants of certain generations. Significant correlations were detected between methylation level and transpiration rate, net photosynthetic rate, peroxidase activity, and instant water utilization efficiency, indicating the involvement of epigenetic regulation in this unpredictable phenotypic variation.

Narrow-sense heritability and PST estimates of DNA methylation in three Populus nigra L. populations under contrasting water availability

2018 ◽  
Vol 14 (5) ◽  
Author(s):  
Mamadou Dia Sow ◽  
Vincent Segura ◽  
Sylvain Chamaillard ◽  
Véronique Jorge ◽  
Alain Delaunay ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Water Availability ◽  
Populus Nigra ◽  
Narrow Sense ◽  
Narrow Sense Heritability

DNA methylation in satellite repeats disorders

2019 ◽  
Vol 63 (6) ◽  
pp. 757-771 ◽  
Author(s):  
Claire Francastel ◽  
Frédérique Magdinier
Keyword(s):  
Dna Methylation ◽  
Human Genome ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Satellite Repeats ◽  
Tremendous Progress ◽  
Genes Encoding ◽  
Dna Elements ◽  
Near Future

Abstract Despite the tremendous progress made in recent years in assembling the human genome, tandemly repeated DNA elements remain poorly characterized. These sequences account for the vast majority of methylated sites in the human genome and their methylated state is necessary for this repetitive DNA to function properly and to maintain genome integrity. Furthermore, recent advances highlight the emerging role of these sequences in regulating the functions of the human genome and its variability during evolution, among individuals, or in disease susceptibility. In addition, a number of inherited rare diseases are directly linked to the alteration of some of these repetitive DNA sequences, either through changes in the organization or size of the tandem repeat arrays or through mutations in genes encoding chromatin modifiers involved in the epigenetic regulation of these elements. Although largely overlooked so far in the functional annotation of the human genome, satellite elements play key roles in its architectural and topological organization. This includes functions as boundary elements delimitating functional domains or assembly of repressive nuclear compartments, with local or distal impact on gene expression. Thus, the consideration of satellite repeats organization and their associated epigenetic landmarks, including DNA methylation (DNAme), will become unavoidable in the near future to fully decipher human phenotypes and associated diseases.

15 LONGITUDINAL EVALUATION OF MUCOSAL DNA METHYLATION IN ULCERATIVE COLITIS SHOWS DISEASE-SPECIFIC CHANGES

2020 ◽  
Vol 158 (3) ◽  
pp. S50-S51
Author(s):  
Suresh Venkateswaran ◽  
Varun Kilaru ◽  
Hari Somineni ◽  
Jason Matthews ◽  
Jeffrey Hyams ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Dna Methylation ◽  
Longitudinal Evaluation ◽  
Disease Specific

Aberrant DNA Methylation Is Associated with Reduced Response to SRC Inhibition in Primary Human Vestibular Schwannoma Cells

2019 ◽  
Author(s):  
Christine Dinh ◽  
Juan Young ◽  
Olena Bracho ◽  
Rahul Mittal ◽  
Denise Yan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Vestibular Schwannoma ◽  
Aberrant Dna Methylation

Global DNA Methylation is influenced by smoking behavior

2007 ◽  
Vol 40 (05) ◽  
Author(s):  
MAN Muschler ◽  
T Hillemacher ◽  
H Frieling ◽  
S Moskau ◽  
A Semmler ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Smoking Behavior ◽  
Global Dna Methylation
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