Plexin domain containing protein 2 is more expressed within the invasive area of human colorectal cancer tissues

Human Cell ◽  
2021 ◽  
Author(s):  
Yasuhiko Hamada ◽  
Akiko Eguchi ◽  
Kyosuke Tanaka ◽  
Masaki Katsurahara ◽  
Noriyuki Horiki ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Human Colorectal Cancer ◽  
Cancer Tissues

scholarly journals miR-142-3p Modulates Cell Invasion and Migration via PKM2-Mediated Aerobic Glycolysis in Colorectal Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
JunYu Ren ◽  
Wenliang Li ◽  
Guoqing Pan ◽  
Fengchang Huang ◽  
Jun Yang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Pyruvate Kinase ◽  
Cancer Cell ◽  
Cell Invasion ◽  
Aerobic Glycolysis ◽  
Cancer Cell Invasion ◽  
Human Colorectal Cancer ◽  
Invasion And Migration ◽  
And Migration ◽  
Cancer Tissues

Decreased expression of miR-142-3p was observed in human cancers. However, the function and mechanism of miR-142-3p in human colorectal cancer remain obscure. The expressions of miR-142-3p in human colorectal cancer tissues and cell lines were measured by RT-qPCR. The effects of miR-142-3p on cell invasion and migration were detected by transwell assays. The efficiency of aerobic glycolysis was determined by glucose consumption and lactate production. Dual-luciferase reporter assays were performed to confirm the correlation between miR-142-3p and pyruvate kinase isozyme M2 (PKM2). The level of PKM2 was assessed by western blotting. Our results showed that the expression of miR-142-3p was decreased both in human colorectal cancer tissues and in cells. Overexpression of miR-142-3p in cell line attenuated colorectal cancer cell invasion and migration. About the underlying mechanism, we found that miR-142-3p modulated aerobic glycolysis via targeting pyruvate kinase M2 (PKM2). In addition, we demonstrated PKM2 and PKM2-mediated aerobic glycolysis contributes to miR-142-3p-mediated colorectal cancer cell invasion and migration. Hence, these data suggested that miR-142-3p was a potential therapeutic target for the treatment of human colorectal cancer.

CircABCC4 Regulates the Proliferation, Migration, and Invasion of Colorectal Cancer SW620 Cells by Targeting Micro RNA-216a-3p

2021 ◽  
Vol 11 (3) ◽  
pp. 548-552
Author(s):  
Yiqian Li ◽  
Haofeng Yuan ◽  
Yibin Chen ◽  
Baoqi Xu ◽  
Yanhong Zhang
Keyword(s):  
Colorectal Cancer ◽  
Micro Rna ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Human Colorectal Cancer ◽  
Cell Behaviors ◽  
Colorectal Cancer Cells ◽  
Sw620 Cells ◽  
Cancer Tissues ◽  
Dual Luciferase Reporter Assay

This work investigates the effect of circABCC4 on the proliferation, migration, and invasion of colorectal cancer SW620 cells; circABCC4’s regulation of miR-216a-3p is also studied. qRT-PCR was used to measure the levels of circABCC4 and miR-216a-3p in colorectal cancer and adjacent tissues. The human colorectal cancer SW620 cells were transfected with different constructs of circABCC4 or miR-216a-3p or both to study their interactions and combined effects on cell behavior. A dual-luciferase reporter experiment tested the targeted relationship between circABCC4 to miR-216a-3p. Furthermore, the behaviors of SW620 cells, such as cell viability, migration, and invasion, were investigated. Also, the proteins related to cell behaviors were investigated with western blotting. Our results showed that colorectal cancer tissues had a higher level of circABCC4 but a lower level miR-216a-3p. The increased level of circABCC4 and the reduced level of miR-216a-3p had analogous influences on the behaviors of SW620 cells, resulting in reduced cell proliferation, migration, and invasion; the levels of related protein were also decreased. Moreover, we found that disrupting miR-548c-3p could reverse the influence of inhibiting circABCC4 on SW620 cells. In addition, the dual-luciferase reporter assay results confirmed the targeting of miR-216a-3p by circABCC4. These data demonstrate that the silencing of circABCC4 may inhibit the proliferation, migration, and invasion of colorectal cancer cells by upregulating miR-548c-3p.

Analysis of gene expression in human colorectal cancer tissues by cDNA array

2002 ◽  
Vol 37 (S14) ◽  
pp. 83-86 ◽  
Author(s):  
Hiroyuki Yamamoto ◽  
Arisa Imsumran ◽  
Hiroshi Fukushima ◽  
Yasushi Adachi ◽  
Yongfen Min ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Cdna Array ◽  
Human Colorectal Cancer ◽  
Cancer Tissues

Prognostic significance of resident CD103+CD8+T cells in human colorectal cancer tissues

2019 ◽  
Vol 121 (5) ◽  
pp. 657-663 ◽  
Author(s):  
Wenwei Hu ◽  
Runzi Sun ◽  
Lujun Chen ◽  
Xiao Zheng ◽  
Jingting Jiang
Keyword(s):  
Colorectal Cancer ◽  
T Cells ◽  
Cd8 T Cells ◽  
Prognostic Significance ◽  
Human Colorectal Cancer ◽  
Cancer Tissues

scholarly journals Purification and identification of monoubiquitin-phosphoglycerate mutase B complex from human colorectal cancer tissues

2001 ◽  
Vol 94 (5) ◽  
pp. 662-668 ◽  
Author(s):  
Teruyuki Usuba ◽  
Yoshio Ishibashi ◽  
Yutaka Okawa ◽  
Tae Hirakawa ◽  
Koji Takada ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Phosphoglycerate Mutase ◽  
Human Colorectal Cancer ◽  
Cancer Tissues

Expression of EGFR,Grb2,p-mTOR and VEGF in human colorectal cancer tissues

2010 ◽  
Vol 29 (7) ◽  
pp. 775-779
Author(s):  
Hong-gang XIANG ◽  
Qiang WANG ◽  
Zhi-qian HU ◽  
Jian XU ◽  
Wei-jun WANG ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Human Colorectal Cancer ◽  
Cancer Tissues

Decoding the S-Nitrosoproteomic Atlas in Individualized Human Colorectal Cancer Tissues Using a Label-Free Quantitation Strategy

2014 ◽  
Vol 13 (11) ◽  
pp. 4942-4958 ◽  
Author(s):  
Yi-Ju Chen ◽  
Wei-Chieh Ching ◽  
Jinn-Shiun Chen ◽  
Tzong-Yi Lee ◽  
Cheng-Tsung Lu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Label Free ◽  
Human Colorectal Cancer ◽  
Cancer Tissues ◽  
Label Free Quantitation
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