Biogreen remediation of chromium-contaminated soil using Pseudomonas sp. (RPT) and neem (Azadirachta indica) oil cake

A regression analysis model was developed to examine the reliability and the acceptability of the sun-dried and room-dried Azadirachta indica leaves for the remediation crude oil contaminated soil in Niger Deltal, Nigeria. An experimental approach was used to monitor the concentration of the total petroleum hydrocarbon (TPH) degradation that was enhanced in the presence of the microbes in the reactor. A regression analysis was performed to evaluate the reaction behavior microbes in reactor for the remediation of the TPH in the presence of sun-dried and room-dried A. indica leaves in sandy and loamy soils contaminated with 100 ml of crude oil. The physicochemical properties of control loamy soil was as; pH (6.75), electric conductivity (10.36 μS/cm), total oxygen (2.99%), total Nitrogen (0.091%), potassium (36.82942%), phosphorus (15.36%) and total bacteria (2.15×102 cfu/g). Whereas, these values were as pH (6.82), electric conductivity (21.48 μS/cm), total oxygen content (1.18%), total nitrogen (0.036%), potassium (24.03681%), phosphorus (5.18%) and total bacteria count (1.86×102 cfu/g) for sandy soil. The inoculant was prepared by blending sun-dried and room-dried A. indica leaves. The blended A. indica leaves in 50 to 100 g range were applied on the polluted soils for 35 days and significant reduction in contaminating agents was observed. The highest remediation was observed in soils treated with 100 g room dried A. indica leaves. A 96% remediation recorded in sandy soil treated with 100 g room dried A. indica leaves as TPH depleted from 35818.69 mg/kg to 1349.109 mg/kg and 92% reduction was observed in loamy soil treated with 100 g dried A. indica leaves and TPH depleted from 48508.92 mg/kg to 3977.739 mg/kg. The regression model developed was successfully employed to predict TPH remediation behavior, which can be applied to monitor remediation of contaminated soil.

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Studies on decolorization of textile dye by using Pseudomonas and bacillus sp from the contaminated effluent soil samples of Kovilpatti, Thoothukudi district of Tamil Nadu

Journal of Applied and Natural Science ◽

10.31018/jans.v11i1.1980 ◽

2019 ◽

Vol 11 (1) ◽

pp. 134-137 ◽

Cited By ~ 1

Author(s):

Kannan D ◽

Renuga Devi ◽

A. G. Murugesan ◽

S. Rajan

Keyword(s):

Contaminated Soil ◽

Tamil Nadu ◽

Soil Samples ◽

Toxic Chemicals ◽

Textile Dye ◽

Pseudomonas Sp ◽

Bacillus Sp ◽

Bacterial Strains ◽

Textile Industries ◽

Source Of Pollution

Textile industries releasing large amount of effluent which contains textile dyes and toxic chemicals and it is one of the major source of pollution also contaminating water bodies. To remove that, bacteria have been of great attention because of their ability to treat effluent. The present study was undertaken to exploit the ability of Pseudomonassp and Bacillus sp from dye contaminated soil samples for bioremediation for dye effluent. Among the bacterial strains used in the study. Pseudomonas sp emerged out to be most potent decolorizer in comparison to Bacillus sp with the degree of decolorization of 90.0 %. Thus, it was concluded that the Pseudomonas sp had highest color removing capacity from contaminated effluent soil samples.

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Rhizobacteria (Pseudomonas sp. SB) assist phytoremediation of oily-sludge-contaminated soil by tall fescue (Testuca arundinacea L.)

Plant and Soil ◽

10.1007/s11104-013-1717-x ◽

2013 ◽

Vol 371 (1-2) ◽

pp. 533-542 ◽

Cited By ~ 33

Author(s):

Wuxing Liu ◽

Jianying Sun ◽

Linlin Ding ◽

Yongming Luo ◽

Mengfang Chen ◽

...

Keyword(s):

Tall Fescue ◽

Contaminated Soil ◽

Oily Sludge ◽

Pseudomonas Sp

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Bioaugmentation of polyethylene succinate-contaminated soil with Pseudomonas sp. AKS2 results in increased microbial activity and better polymer degradation

Environmental Science and Pollution Research ◽

10.1007/s11356-012-1080-0 ◽

2012 ◽

Vol 20 (3) ◽

pp. 1318-1326 ◽

Cited By ~ 25

Author(s):

Prosun Tribedi ◽

Alok K. Sil

Keyword(s):

Microbial Activity ◽

Contaminated Soil ◽

Polymer Degradation ◽

Pseudomonas Sp

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Biosurfactant-producing microorganism Pseudomonas sp. SB assists the phytoremediation of DDT-contaminated soil by two grass species

Chemosphere ◽

10.1016/j.chemosphere.2017.04.123 ◽

2017 ◽

Vol 182 ◽

pp. 137-142 ◽

Cited By ~ 21

Author(s):

Beibei Wang ◽

Qingling Wang ◽

Wuxing Liu ◽

Xiaoyan Liu ◽

Jinyu Hou ◽

...

Keyword(s):

Contaminated Soil ◽

Grass Species ◽

Pseudomonas Sp

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Penghilangan Senyawa Fenol Oleh Bakteri Yang Diisolasi dari Area Pertambangan Minyak Bumi

Jurnal Teknologi Lingkungan ◽

10.29122/jtl.v17i2.1067 ◽

2016 ◽

Vol 17 (2) ◽

pp. 126 ◽

Cited By ~ 1

Author(s):

Joko Prayitno

Keyword(s):

Removal Efficiency ◽

Contaminated Soil ◽

Phenol Removal ◽

Pseudomonas Sp ◽

Bacillus Sp ◽

Bacterial Strains

The aim of this research was to study the effectiveness of local bacterial strains from oil-contaminated soil to degrade phenol. The study consisted of two experiments, using six individual strains and using mix of strains. Bacterial strains used in the first experiment were 1.3, 3.3 dan 8.2.1 (Bacillus sp.), strain 3.2 (Propionibacterium), strain 3.4 (Pseudomonas sp.), and strain 8.1.2 (Enterobacter sp.).Bacterial strains used in the second experiment were mix of all six strains (K6) and mix of three strains (K3) consisted of strain 3.4, 8.1.2 and 8.2.1 with the same ratio. The experiments were conducted in 100 mL Bushnell and Haas medium containing 300-400 ppm phenol for three days.Three strains (strain 3.4, 8.1.2, dan 8.2.1) had the highest phenol removal efficiency at day 3, i.e. 99-100%. COD values were decreased to 345-393 mg/L or 56-61.3% by those three strains. Mix culture K6 effectively removed phenol form the medium, but COD value decreased to only 56.7%. The fate of COD decrease was not the same as phenol removal by these strains (either in idividual or mix cultures), because phenol was degraded into intermediate compounds.

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INDUCED DEGRADATION OF CRUDE OIL POLLUTED SOIL BY MICROBIAL AUGMENTATION

Journal of Engineering Studies and Research ◽

10.29081/jesr.v23i4.73 ◽

2017 ◽

Vol 23 (4) ◽

pp. 37-44

Author(s):

SYLVESTER UWADIAE ◽

EMIKE OMOAYENA

Keyword(s):

Crude Oil ◽

Soil Sample ◽

Contaminated Soil ◽

Polluted Soil ◽

Soil Samples ◽

The Other ◽

Pseudomonas Sp ◽

Hydrocarbon Content ◽

Percentage Removal

This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: Bacillus substilis and Pseudomonas sp. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5 (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.

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Интенсификация биоремедиации нефтезагрязненных почв с использованием природного консорциума микроорганизмов

SCIENCE & TECHNOLOGIES OIL AND OIL PRODUCTS PIPELINE TRANSPORTATION ◽

10.28999/2541-9595-2021-11-5-582-589 ◽

2021 ◽

pp. 582-589

Author(s):

Kalamkas B. Oralbayeva ◽

Aliya K. Yernazarova ◽

Medet E. Kundybayev ◽

Spandiar A. Seitkulov ◽

Aida S. Kistaubayeva

Keyword(s):

Pseudomonas Aeruginosa ◽

Contaminated Soil ◽

Contaminated Soils ◽

Climatic Conditions ◽

Mineral Fertilizers ◽

Pseudomonas Sp ◽

Bacterial Strains ◽

Biological Product ◽

Metabolic Potential ◽

Alcaligenes Denitrificans

Currently biological methods of cleaning oily soils significantly developed as the most accessible and effective. In their basis – the use of hydrocarbon-oxidizing microorganisms (HOM) metabolic potential. At the same time, as practice shows, existing preparations containing HOM are not effective enough in extreme soil-climatic conditions, in this connection the search for indigenous strains of oil-oxidizing bacteria and the development of new biological preparations are of current interest. The aim of this work is to study the effectiveness of HOM strains for restoring the biological activity of oil-contaminated soil. A study of the microflora of unpolluted and oil-contaminated soil taken from the experimental site of the “Shymkent” oil pumping station of “KazTransOil” JSC was carried out: the total number of microorganisms was 210·106cells/g and 130·106 cells/g, respectively. From 16 НOM strains isolated from oil-contaminated soil, 3 bacterial strains were selected to create an association and use as a biological product-oil destructor – P12, P16, A8. Based on the results of genotyping, strains P12 and P16 correspond to the species Pseudomonas aeruginosa, strain A8 belongs to the species Alcaligenes denitrificans. The associations of Pseudomonas sp. P16, Pseudomonas sp. P12 and Alcaligenes sp. A8 showed better growth in comparison with monocultures on a nutrient medium with hydrocarbons. Bioremediation of artificially oil polluted site process, which carried out for 120 days using a biological product created on the basis of the association of strains P12, P16 and A8, decreased the content of hydrocarbons 3.4 times, and with additional application of mineral fertilizers (ammophos) – 4.6 times. Thus, proved the expediency of using HOM strains P12, P16, A8 for the purpose of bioremediation of oil-contaminated soils. The results of the study can be used to improve economical and environmental efficiency of work when carrying out measures to eliminate the consequences of oil spills on the ground. Биологические методы очистки нефтесодержащих почв в настоящее время имеют существенное развитие как наиболее доступные и эффективные. В их основе – использование метаболического потенциала углеводородокисляющих микроорганизмов (УОМ). При этом, как показывает практика, существующие препараты, содержащие УОМ, недостаточно эффективны в экстремальных почвенно-климатических условиях, в связи с чем актуальными являются вопросы поиска аборигенных штаммов нефтеокисляющих бактерий и разработка новых биопрепаратов. Цель работы – исследование эффективности штаммов УОМ для восстановления биологической активности нефтезагрязненного почвогрунта. Проведено изучение микрофлоры незагрязненной и нефтезагрязненной почвы, отобранной с экспериментального участка ГНПС «Шымкент» АО «КазТрансОйл»: общее количество микроорганизмов составило 210·106 кл/г и 130·106кл/г, соответственно. Из выделенных из нефтезагрязненной почвы 16 штаммов УОМ были отобраны три штамма бактерий для создания ассоциации и применения в качестве биопрепарата-нефтедеструктора – Р12, Р16, А8. По итогам генотипирования установлено, что штаммы Р12 и Р16 соответствуют виду Pseudomonas aeruginosa, штамм А8 относится к виду Alcaligenes denitrificans. Установлено, что в составе ассоциаций штаммы Pseudomonas sp. P16, Pseudomonas sp. P12 и Alcaligenes sp. A8. показали лучший по сравнению с монокультурами рост на питательной среде с углеводородами. В процессе биоремедиации участка с искусственным нефтезагрязнением, проводимой в течение 120 суток с использованием биопрепарата, созданного на основе ассоциации штаммов P12, P16 и A8, содержание углеводородов снизилось в 3,4 раза, а при дополнительном внесении минеральных удобрений (аммофоса) – в 4,6 раза. Таким образом, доказана целесообразность использования штаммов УОМ Р12, Р16, А8 с целью биоремедиации нефтезагрязненных почв. Результаты исследования могут быть использованы для повышения экономической и экологической эффективности работ при проведении мероприятий по ликвидации последствий разливов нефти на почве.

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