Quantity and transmission efficiency of an isolate of the Potato virus Y–Wilga (PVYN−Wi) by aphid species reared on different host plants

2019 ◽  
Vol 126 (6) ◽  
pp. 529-534
Author(s):  
Saman Bahrami Kamangar ◽  
Clauvis Nji Tizi Taning ◽  
Kris De Jonghe ◽  
Guy Smagghe
Keyword(s):  
Potato Virus ◽  
Host Plants ◽  
Potato Virus Y ◽  
Transmission Efficiency ◽  
Aphid Species

scholarly journals Transmission Efficiency of Potato virus Y strains PVYO and PVYN-Wi by Five Aphid Species

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1279-1283 ◽  
Author(s):  
A. F. S. Mello ◽  
R. A. Olarte ◽  
S. M. Gray ◽  
K. L. Perry
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Rhopalosiphum Padi ◽  
Potato Production ◽  
Aphid Transmission ◽  
Transmission Efficiency ◽  
Aphid Species ◽  
Age And Growth ◽  
Potato Seed ◽  
True Potato Seed

Potato virus Y (PVY) is a reemerging problem in potato production in North America. Although the “ordinary” strain, PVYO, is still the dominant isolate in U.S. seed potatoes, the recombinant strain of the virus PVYN-Wi (= PVYN:O) has become widespread. An increase in the prevalence of a PVY strain could be due to differences in the efficiency of transmission by aphid vectors. The transmission efficiency by a clone of Myzus persicae was determined for five isolates each of PVYO and PVYN-Wi. An aphid transmission assay was developed based on the use of potato seedlings from true potato seed, allowing for greater control of plant age and growth stage. No apparent differences in transmission by M. persicae were observed. Single isolates of PVYO and PVYN-Wi were tested for their ability to be transmitted from potato to potato by five aphid species: Aphis glycines, A. gossypii, A. nasturtii, M. persicae, and Rhopalosiphum padi. Both PVY isolates showed a similar transmission phenotype in being transmitted efficiently by M. persicae but very poorly or not at all by A. glycines, A. gossypii, and R. padi. The aphid A. nasturtii transmitted both isolates with an intermediate level of efficiency. The data do not support a model for a differential aphid transmissibility being responsible for the increase in the prevalence of PVYN-Wi.

scholarly journals Modulation of Aphid Vector Activity by Potato virus Y on In Vitro Potato Plants

Plant Disease ◽  
2012 ◽  
Vol 96 (1) ◽  
pp. 82-86 ◽  
Author(s):  
S. Boquel ◽  
C. Delayen ◽  
A. Couty ◽  
P. Giordanengo ◽  
A. Ameline
Keyword(s):  
Feeding Behavior ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Aphid Species ◽  
Aphis Fabae ◽  
Plant Colonization ◽  
Electrical Penetration Graph ◽  
Vector Activity ◽  
Aphid Feeding

The effects of the infection of potato (Solanum tuberosum) plants by the nonpersistent Potato virus Y (PVY) were studied on the host plant colonization behavior of different colonizing (Myzus persicae) and noncolonizing (Aphis fabae, Brevicoryne brassicae, and Sitobion avenae) aphid species. The underlying questions of this study were to know how aphids respond when faced with PVY-infected plants and whether plant infection can modify the aphid behavior involved in PVY spread. Short-range orientation behavior was observed using a dual-choice set-up and aphid feeding behavior was monitored using the electrical penetration graph technique. None of the aphid species discriminated between healthy and PVY-infected plants. Nevertheless, most individuals of M. persicae landed on and probed only in one plant whereas noncolonizing aphid species exhibited interplant movements. Study of the aphid feeding behavior showed that PVY infection essentially modified phloem and xylem ingestion. M. persicae and S. avenae exhibited an increased duration of phloem phases on PVY-infected plants whereas A. fabae showed a decreased duration of phloem phases that benefited from an increased duration of xylem ingestion phases. None of these parameters were changed in B. brassicae. These data present evidence that aphids can respond to plants infected by nonpersistent viruses. Such behavioral modifications are discussed within the context of PVY spread in potato crops.

COMPARATIVE TRANSMISSION OF POTATO VIRUS Y BY FOUR APHID SPECIES THAT INFEST POTATO

1953 ◽  
Vol 31 (4) ◽  
pp. 333-341 ◽  
Author(s):  
R. H. E. Bradley ◽  
D. W. Rideout
Keyword(s):  
Feeding Behavior ◽  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Tobacco Plant ◽  
Aphid Species ◽  
Vector Efficiency

Single aphids of four species were observed with a hand-lens until each had touched its proboscis once on a tobacco plant infected with potato virus Y and then once on a healthy tobacco plant. The time that the proboscis remained touching the plants in each case was recorded. This procedure required only a few minutes for each aphid, and sometimes it was completed in less than a minute. During this process, the successful transmissions of potato virus Y by single aphids of Myzus persicae (Sulz.), Aphis abbreviata Patch, Macrosiphum solanifolii (Ashm), and Myzus solani (Kltb.) were 55, 31, 9, and 4% respectively. Vector efficiency was not due to difference in feeding behavior of these species. When single infective aphids were transferred to a series of five plants at five-minute intervals, M. persicae caused more infections and remained infective longer than A. abbreviata.

scholarly journals The use of test tube plantlets for the assessment of Potato virus Y transmission by Myzus persicae and Aphis glycines

2012 ◽  
Vol 91 ◽  
pp. 15-18 ◽  
Author(s):  
Xianzhou Nie ◽  
Marie-Andrée Giguère ◽  
Yvan Pelletier
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Transmission Rate ◽  
Transmission Efficiency ◽  
Test Tube ◽  
Aphis Glycines ◽  
Colonizing Species ◽  
Potted Plants

Test tube plantlets were used to assess the transmission efficiency of Potato virus Y (PVY) from and to potato plants by the potato colonizing species green peach aphid (Myzus persicae) and the non-potato colonizing species soybean aphid (Aphis glycines). Similar levels of transmission of PVY by M. persicae were observed in the test tube plantlets and potted plants, demonstrating the reliability of this test for PVY transmission efficiency assay for aphids. The assay was then used to assess the transmission of PVYO and PVYN:O by M. persicae and A. glycines with two virus acquisition regimes, one with 5-min continuous probing and the other with 1-h acquisition access. The M. persicae mediated-transmission rate was 24.1% and 51.7% for PVYO and PVYN:O, respectively, under the 5-min acquisition regime; under the same acquisition regime, A. glycines led to 0.0% and 1.7% infection rates for PVYO and PVYN:O, respectively. Under the 1-h acquisition regime, no infection was observed except for PVYN:O by M. persicae, which exhibited an infection rate of 3.4%.

Assessing aphids potato virus Y-transmission efficiency: A new approach

2011 ◽  
Vol 178 (1-2) ◽  
pp. 63-67 ◽  
Author(s):  
Sébastien Boquel ◽  
Arnaud Ameline ◽  
Philippe Giordanengo
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Transmission Efficiency ◽  
New Approach

scholarly journals Helper Component Mutations in Nonconserved Residues Associated with Aphid Transmission Efficiency of a Pepper Isolate of Potato Virus Y

1999 ◽  
Vol 89 (12) ◽  
pp. 1176-1181 ◽  
Author(s):  
César Llave ◽  
Belén Martínez ◽  
J. R. Díaz-Ruíz ◽  
D. López-Abella
Keyword(s):  
Amino Acid ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Aphid Transmission ◽  
Transmission Efficiency ◽  
Binding Assays ◽  
Helper Component ◽  
Rich Domain ◽  
Pepper Isolate

The aphid transmission properties of a pepper isolate of potato virus Y belonging to the pathotype 1–2 (PVY 1–2) have been characterized. PVY 1–2 was not transmitted in plant-to-plant experiments, although purified virus particles were efficiently transmitted when supplemented with heterologous helper component (HC) of the transmissible isolate PVY 0 AT through membrane acquisition assays, indicating that its coat protein was functional in transmission. Additionally, virions of PVY 1–2 were able to bind to different HCs in in vitro binding assays. Analysis of the sequence of the PVY 1–2 HC gene and comparison with that of PVY 0 AT revealed 19 nucleotide differences, but only 2 resulted in amino acid changes, one of which induced a change of charge. Neither of these two amino acid changes occurred within the cysteine-rich domain, nor did they coincide with conserved motifs of the HC protein known to be involved in aphid transmission and which are present in all known potyvi-ruses. However, both changes are located in positions highly conserved among PVY strains. The possible role of both mutations on the activity of the PVY 1–2 HC in aphid transmission is discussed.

scholarly journals Contribution to the study of potato aphids in Greece

2017 ◽  
Vol 4 ◽  
pp. 11 ◽  
Author(s):  
P.C. Panayotoy ◽  
N. Katis
Keyword(s):  
Potato Virus ◽  
High Resistance ◽  
Potato Virus Y ◽  
Aphid Species ◽  
Potato Plants ◽  
Potato Foliage ◽  
Potato Aphids ◽  
First Time

Four aphid species were identified in the Metsovo potato center, Greece. Rhopalosiphoninus latysiphon, infesting subterranean parts of potato, is reported for the first time in Greece, while Aphis frangulae, infesting the potato foliage, is reported for the first time on potato plants in Greece. High resistance to methamidophos was observed with the latter aphid species. Potato virus Y (PVY) was transmitted by all four species of aphids

Potato Virus Y transmission efficiency for some common aphids in Idaho

2003 ◽  
Vol 80 (2) ◽  
pp. 87-91 ◽  
Author(s):  
Susan E. Halbert ◽  
Dennis L. Corsini ◽  
Monica A. Wiebe
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Transmission Efficiency

scholarly journals Transmission of Potato virus Y in Tobacco Plants by Myzus persicae nicotianae and M. persicae s.str.

Plant Disease ◽  
2006 ◽  
Vol 90 (6) ◽  
pp. 777-782 ◽  
Author(s):  
Olympia M. Kanavaki ◽  
John T. Margaritopoulos ◽  
Nikolaos I. Katis ◽  
Panagiotis Skouras ◽  
John A. Tsitsipis
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Host Selection ◽  
Potato Virus Y ◽  
Virus Transmission ◽  
Transmission Efficiency ◽  
Behavioral Differences ◽  
Tobacco Plants ◽  
Probing Behavior ◽  
Inoculation Access Period

The transmission efficiency of Potato virus Y N (PVYN) from and to tobacco plants by Myzus persicae nicotianae, a tobacco specialist, and M. persicae s.str. (Hemiptera: Aphididae), a generalist, was evaluated. In direct transmission tests, 1,004 spring migrants collected as nymphs from peach and 1,434 wingless females from 12 clonal lineages of both taxa were examined. Both the winged and wingless females were starved before being transferred individually for a 3-min acquisition period on infected plants and then moved to healthy plants for a 10-min inoculation access period. Little variation in transmission efficiency was observed. Although not statistically significant, M. persicae nicotianae was slightly more effective. This may suggest similar genetic properties (e.g., virion receptors in mouthparts) between the taxa in relation to virus transmission. M. persicae s.str. transmitted the virus less efficiently when a shorter inoculation period was given. This may indicate differences in probing behavior during the early phase of host selection. Nevertheless, behavioral differences related to host selection have been found using arena tests with infected and healthy tobacco plants where 180 winged females from each taxon were tested. M. persicae s.str. had significantly higher propensity to transmit PVYN than M. persicae nicotianae (31.9 versus 15.3%).

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