scholarly journals The use of test tube plantlets for the assessment of Potato virus Y transmission by Myzus persicae and Aphis glycines

2012 ◽  
Vol 91 ◽  
pp. 15-18 ◽  
Author(s):  
Xianzhou Nie ◽  
Marie-Andrée Giguère ◽  
Yvan Pelletier
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Transmission Rate ◽  
Transmission Efficiency ◽  
Test Tube ◽  
Aphis Glycines ◽  
Colonizing Species ◽  
Potted Plants

Test tube plantlets were used to assess the transmission efficiency of Potato virus Y (PVY) from and to potato plants by the potato colonizing species green peach aphid (Myzus persicae) and the non-potato colonizing species soybean aphid (Aphis glycines). Similar levels of transmission of PVY by M. persicae were observed in the test tube plantlets and potted plants, demonstrating the reliability of this test for PVY transmission efficiency assay for aphids. The assay was then used to assess the transmission of PVYO and PVYN:O by M. persicae and A. glycines with two virus acquisition regimes, one with 5-min continuous probing and the other with 1-h acquisition access. The M. persicae mediated-transmission rate was 24.1% and 51.7% for PVYO and PVYN:O, respectively, under the 5-min acquisition regime; under the same acquisition regime, A. glycines led to 0.0% and 1.7% infection rates for PVYO and PVYN:O, respectively. Under the 1-h acquisition regime, no infection was observed except for PVYN:O by M. persicae, which exhibited an infection rate of 3.4%.

Role of Hairy Nightshade in the Transmission of Different Potato virus Y Strains on Solanum tuberosum (L.)

2010 ◽  
Vol 11 (1) ◽  
pp. 38 ◽  
Author(s):  
Felix A. Cervantes ◽  
Juan M. Alvarez
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Transmission Rate ◽  
Solanum Tuberosum L ◽  
Macrosiphum Euphorbiae ◽  
Invasive Weed ◽  
Potato Plants ◽  
Tuber Necrosis

The complexity of the Potato virus Y (PVY) (Potyviridae: Potyvirus) pathosystem is affected by the presence of several virus strains that differ in their ability to produce tuber necrosis and by the presence of an alternate host that could increase the amount of inoculum in potato fields. Solanum sarrachoides (Sendtner) is an invasive weed from South America present in Pacific Northwest potato agro-ecosystems. It serves as reservoir of PVY and its most efficient vectors: the green peach aphid, Myzus persicae (Sulzer), and the potato aphid, Macrosiphum euphorbiae (Thomas). The role of S. sarracoides as vector and virus reservoir in PVY epidemiology was investigated through a series of laboratory and greenhouse experiments. We studied the symptoms produced in S. sarracoides upon infection with necrotic and non-necrotic strains of PVY and looked at the percentage of infection and titer accumulation of these strains. PVY infection in S. sarrachoides produced symptoms similar to those produced in PVY-infected potato plants. Mottling and yellowing were the main symptoms of infection observed in S. sarrachoides plants, especially by PVYO and PVYNTN infection. Greenhouse transmission studies revealed that PVY-infected S. sarrachoides increased the transmission rate of PVY necrotic strains by M. persicae. The necrotic strain PVYNTN reached higher titer in S. sarrachoides than in potato plants when compared to PVYO and PVYN:O These findings have broadened our understanding of the role and importance of S. sarrachoides in the PVY epidemiology in the potato ecosystems and could potentially be included in the development or optimization of virus management programs. Accepted for publication 15 March 2010. Published 26 May 2010.

The cuticle protein MPCP2 is involved in Potato virus Y transmission in the green peach aphid Myzus persicae

2019 ◽  
Vol 126 (4) ◽  
pp. 351-357 ◽  
Author(s):  
Saman Bahrami Kamangar ◽  
Olivier Christiaens ◽  
Clauvis N. T. Taning ◽  
Kris De Jonghe ◽  
Guy Smagghe
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Cuticle Protein

STUDIES OF THE MECHANISM OF TRANSMISSION OF POTATO VIRUS Y BY THE GREEN PEACH APHID, MYZUS PERSICAE (SULZ.) (HOMOPTERA: APHIDAE)

1954 ◽  
Vol 32 (2) ◽  
pp. 64-73 ◽  
Author(s):  
R. H. E. Bradley
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Infected Plant ◽  
Test Plants

Wingless adults of Myzus persicae (Sulz.) usually ceased to be infective within minutes and always within hours after leaving tobacco infected with potato virus Y, the time being longer when the aphids were kept from feeding than when they fed, and aphids that were kept from feeding remained infective longer at 2 °C. than at 35 °C. After one to four hours without food, over 80% of M. persicae made initial feeding punctures that lasted less than a minute, and about 70% of these aphids transmitted potato virus Y after one such feeding puncture on an infected plant. Though virus was acquired by aphids during feeding punctures as brief as five seconds, those that made feeding punctures lasting 11–60 sec. were the most likely to become infective. The percentage of aphids that transmitted potato virus Y decreased when the stylets were inserted into infected plants for over a minute, and none of the aphids transmitted the virus after the stylets had been inserted over 20 min. The highest percentage of aphids transmitted potato virus Y when they were transferred to test plants immediately after a single brief feeding puncture on an infected plant. The percentage of aphids that transmitted the virus decreased when they spent 10 min. or longer on infected plants; also, the probability of their becoming infective during a single feeding puncture decreased by about one third during the first 10 min. they fed after one to four hours without food. Even when conditions were suitable, about 25% of M. persicae failed to transmit potato virus Y. Yet those that failed to transmit the virus in one infection trial transmitted it as readily in a second trial as those that transmitted it in the first.

scholarly journals Transmission of Potato virus Y in Tobacco Plants by Myzus persicae nicotianae and M. persicae s.str.

Plant Disease ◽  
2006 ◽  
Vol 90 (6) ◽  
pp. 777-782 ◽  
Author(s):  
Olympia M. Kanavaki ◽  
John T. Margaritopoulos ◽  
Nikolaos I. Katis ◽  
Panagiotis Skouras ◽  
John A. Tsitsipis
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Host Selection ◽  
Potato Virus Y ◽  
Virus Transmission ◽  
Transmission Efficiency ◽  
Behavioral Differences ◽  
Tobacco Plants ◽  
Probing Behavior ◽  
Inoculation Access Period

The transmission efficiency of Potato virus Y N (PVYN) from and to tobacco plants by Myzus persicae nicotianae, a tobacco specialist, and M. persicae s.str. (Hemiptera: Aphididae), a generalist, was evaluated. In direct transmission tests, 1,004 spring migrants collected as nymphs from peach and 1,434 wingless females from 12 clonal lineages of both taxa were examined. Both the winged and wingless females were starved before being transferred individually for a 3-min acquisition period on infected plants and then moved to healthy plants for a 10-min inoculation access period. Little variation in transmission efficiency was observed. Although not statistically significant, M. persicae nicotianae was slightly more effective. This may suggest similar genetic properties (e.g., virion receptors in mouthparts) between the taxa in relation to virus transmission. M. persicae s.str. transmitted the virus less efficiently when a shorter inoculation period was given. This may indicate differences in probing behavior during the early phase of host selection. Nevertheless, behavioral differences related to host selection have been found using arena tests with infected and healthy tobacco plants where 180 winged females from each taxon were tested. M. persicae s.str. had significantly higher propensity to transmit PVYN than M. persicae nicotianae (31.9 versus 15.3%).

scholarly journals Potato virus Y Transmission Efficiency from Potato Infected with Single or Multiple Virus Strains

2017 ◽  
Vol 107 (4) ◽  
pp. 491-498 ◽  
Author(s):  
Shaonpius Mondal ◽  
Yu-Hsuan Lin ◽  
Juliet E. Carroll ◽  
Erik J. Wenninger ◽  
Nilsa A. Bosque-Pérez ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Potato Crop ◽  
Potato Cultivar ◽  
Aphid Transmission ◽  
The United States ◽  
Transmission Efficiency ◽  
Recombinant Strains ◽  
Virus Strains

There has been a recent shift in the prevalence of Potato virus Y (PVY) strains affecting potato with the ordinary strain PVYO declining and the recombinant strains PVYNTN and PVYN:O emerging in the United States. Multiple PVY strains are commonly found in potato fields and even in individual plants. Factors contributing to the emergence of the recombinant strains are not well defined but differential aphid transmission of strains from single and mixed infections may play a role. We found that the transmission efficiencies by Myzus persicae, the green peach aphid, of PVYNTN, PVYN:O, and PVYO varied depending on the potato cultivar serving as the virus source. Overall transmission efficiency was highest from sources infected with three virus strains, whereas transmission from sources infected with one or two virus strains was not significantly different. Two strains were concomitantly transmitted by individual aphids from many of the mixed-source combinations, especially if PVYO was present. Triple-strain infections were not transmitted by any single aphid. PVYO was transmitted most efficiently from mixed-strain infection sources. The data do not support the hypothesis that differential transmission of PVY strains by M. persicae is a major contributing factor in the emergence of recombinant PVY strains in the U.S. potato crop.

SOME WAYS IN WHICH A PARAFFIN OIL IMPEDES APHID TRANSMISSION OF POTATO VIRUS Y

1963 ◽  
Vol 9 (3) ◽  
pp. 369-380 ◽  
Author(s):  
R. H. E. Bradley
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Laboratory Tests ◽  
Potato Virus Y ◽  
Aphid Transmission ◽  
The Third ◽  
Paraffin Oil

In laboratory tests with a light white paraffin oil, transmission of potato virus Y by viruliferous adults of Myzus persicae (Sulz.) was impeded by (a) allowing aphids to probe on a leaf coated with oil, (b) manually touching the end of the labium to an oil-coated leaf, or (c) inserting the bare stylets directly into the oil. The first two of these but not the third also impeded for some minutes the uptake and transmission of virus Y by nonviruliferous aphids. Oil sprayed on plants kept in the laboratory continued to cause these effects for some weeks. If infected plants, used as virus sources for aphids, were coated with oil, transmission from them was impeded. It is still not clear why oil impedes transmission. But the results give good reasons to believe that oil can be used in the field to prevent spread of certain aphid-borne viruses.

Dispersion of Myzus persicae and transmission of Potato virus Y under elevated CO2 atmosphere

2018 ◽  
Vol 166 (5) ◽  
pp. 380-385 ◽  
Author(s):  
Emilie Bosquee ◽  
Antoine Boullis ◽  
Morgane Bertaux ◽  
Frédéric Francis ◽  
François J. Verheggen
Keyword(s):  
Elevated Co2 ◽  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Co2 Atmosphere

scholarly journals Transmission Efficiency of Potato virus Y strains PVYO and PVYN-Wi by Five Aphid Species

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1279-1283 ◽  
Author(s):  
A. F. S. Mello ◽  
R. A. Olarte ◽  
S. M. Gray ◽  
K. L. Perry
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Rhopalosiphum Padi ◽  
Potato Production ◽  
Aphid Transmission ◽  
Transmission Efficiency ◽  
Aphid Species ◽  
Age And Growth ◽  
Potato Seed ◽  
True Potato Seed

Potato virus Y (PVY) is a reemerging problem in potato production in North America. Although the “ordinary” strain, PVYO, is still the dominant isolate in U.S. seed potatoes, the recombinant strain of the virus PVYN-Wi (= PVYN:O) has become widespread. An increase in the prevalence of a PVY strain could be due to differences in the efficiency of transmission by aphid vectors. The transmission efficiency by a clone of Myzus persicae was determined for five isolates each of PVYO and PVYN-Wi. An aphid transmission assay was developed based on the use of potato seedlings from true potato seed, allowing for greater control of plant age and growth stage. No apparent differences in transmission by M. persicae were observed. Single isolates of PVYO and PVYN-Wi were tested for their ability to be transmitted from potato to potato by five aphid species: Aphis glycines, A. gossypii, A. nasturtii, M. persicae, and Rhopalosiphum padi. Both PVY isolates showed a similar transmission phenotype in being transmitted efficiently by M. persicae but very poorly or not at all by A. glycines, A. gossypii, and R. padi. The aphid A. nasturtii transmitted both isolates with an intermediate level of efficiency. The data do not support a model for a differential aphid transmissibility being responsible for the increase in the prevalence of PVYN-Wi.

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