Detection of Tomato brown rugose fruit virus (ToBRFV) in solanaceous plants in Mexico

Author(s):  
Anuar Ahmed Magaña-Álvarez ◽  
Daisy Pérez-Brito ◽  
Brenda Yazmín Vargas-Hernández ◽  
José Abrahán Ramírez-Pool ◽  
Leandro Alberto Núñez-Muñoz ◽  
...  
Keyword(s):  
2011 ◽  
Vol 12 (1) ◽  
pp. 34 ◽  
Author(s):  
Craig G. Webster ◽  
William W. Turechek ◽  
H. Charles Mellinger ◽  
Galen Frantz ◽  
Nancy Roe ◽  
...  

To the best of our knowledge, this is the first report of GRSV infecting tomatillo and eggplant, and it is the first report of GRSV infecting pepper in the United States. This first identification of GRSV-infected crop plants in commercial fields in Palm Beach and Manatee Counties demonstrates the continuing geographic spread of the virus into additional vegetable production areas of Florida. This information indicates that a wide range of solanaceous plants is likely to be infected by this emerging viral pathogen in Florida and beyond. Accepted for publication 27 June 2011. Published 25 July 2011.


Parasitology ◽  
1945 ◽  
Vol 36 (3-4) ◽  
pp. 209-210 ◽  
Author(s):  
Kenneth M. Smith

It has been shown that Atropa belladonna acts as a symptomless carrier of Hyosoyamus Virus I. Some symptoms caused by this virus on other solanaceous plants are described.A naturally occurring virus complex in Hyoscyamus niger was found to consist of Solanum Virus I (potato virus X) and Brassica Virus I. During the course of the investigation it was found that potato virus X forms local lesions on the cotyledons of ridge cucumber without systemic infection and that Brassica Virus I behaves similarly on the inoculated leaves of Nicotiana sylvestris.


Tobacco mosaic was the first disease shown to be caused by a filter-passing virus, and Beijerinck (1898) suggested as its cause a “contagium vivum fluidum", Since then many other theories have been advanced, but there has been little positive evidence to indicate whether the virus more nearly resembled organisms such as small bacteria or chemical molecules such as the larger proteins. Recently, however, Stanley has isolated from tobacco (1936a) and tomato (Stanley and Loring 1936) plants suffering from mosaic a protein which he describes as crystalline and as possessing the properties of the virus. When susceptible plants were inoculated with this protein at a dilution of 10 -9 they developed typical symptoms of the disease. The protein was obtained from infective sap by repeated precipitation with 40% saturated ammonium sulphate solution, and by adsorption on and washing from celite. The “crystals” described by Stanley were small needles produced by precipitation with acid ammonium sulphate. A number of statements in Stanley’s earlier paper (1935 more especially those dealing with the nitrogen content and the serological activity, made us doubt the purity of his product, and preliminary experiments with methods similar to those used for the preparation of suspensions of potato virus “X” (Bawden and Pirie 1936) gave us products with much higher precipitation end-points with antisera than those claimed by Stanley. We have now exchanged material with Dr. Stanley and find no gross differences in the activities of our respective products. We have found, however, that by further purification the protein in neutral solution can be obtained in liquid crystalline states. Also, as will be shown later, there are considerable differences in the chemical descriptions given of the virus protein; some of these differences have already been resolved, and others presumably will be by future work.


2004 ◽  
Vol 17 (5) ◽  
pp. 475-483 ◽  
Author(s):  
Kappei Kobayashi ◽  
Thomas Hohn

Cauliflower mosaic virus (CaMV) transactivator/viroplasmin (Tav) is a multifunctional protein essential for basic replication of CaMV. It also plays a role in viral pathogenesis in crucifer and solanaceous host plants. Deletion mutagenesis revealed that N- and C-terminal parts of Tav are not essential for CaMV replication in transfected protoplasts. Two deletion mutants having only minimal defects in basic replication were infectious in turnips but only with highly attenuated virulence. This was shown to be due to delayed virus spread within the inoculated leaves and to the upper leaves. Unlike the wild-type virus, the mutant viruses successfully spread locally without inducing a host defense response in inoculated Datura stramonium leaves, but did not spread systemically. These results provide the first evidence that a Tav domain required for avirulence function in solanaceous plants is not essential for CaMV infectivity but has a role in viral virulence in susceptible hosts.


2005 ◽  
Vol 34 (4) ◽  
pp. 689-692 ◽  
Author(s):  
Alexsandra A. Rosa ◽  
Manoel G.C. Gondim Jr. ◽  
Komi K.M. Fiaboe ◽  
Gilberto J. de Moraes ◽  
Markus Knapp

Plant Disease ◽  
2021 ◽  
Author(s):  
Yi-Wen Tseng ◽  
Chien-Fu Wu ◽  
Chia-Hwa Lee ◽  
Chung Jan Chang ◽  
Yuh-Kun Chen ◽  
...  

A number of viruses and viroids infect solanaceous plants causing severe yield losses. Several seed-borne viroids are currently listed as quarantine pathogens in many countries. Among them, columnea latent viroid (CLVd), pepper chat fruit viroid (PCFVd), potato spindle tuber viroid (PSTVd), tomato apical stunt viroid (TASVd), tomato chlorotic dwarf viroid (TCDVd), and tomato planta macho viroid (TPMVd) are of major concerns. The objective of this study was to design and test universal primers that could be used to detect six viroids in solanaceous plants using one-step RT-PCR and reverse transcription loop-mediated isothermal amplification (RT-LAMP). Results revealed that a pair of degenerate primers could be used in a one-step RT-PCR to amplify six pospiviroids from Solanaceae seeds and plants. Moreover, five primers were designed and used in RT-LAMP to amplify six pospiviroids. The minimal concentration of viroid RNA required for a successful detection varied, ranging from one femtogram to 10 nanograms, depending on the species of viroid and detection method. In general, RT-LAMP was more sensitive than RT-PCR but both assays were rapid and highly sensitive tools to detect six pospiviroids. Detection methods currently in use for these viroids require at least two different sets of primers. The assays developed in this research could facilitate to screen a large number of solanaceous plants and seeds intended for import and export.


Author(s):  

Abstract A new distribution map is provided for Heliothis assulta[Helicoverpa assulta] Gn. (Lep., Noctuidae) (Cape Gooseberry Budworm). Host Plants: Maize, Physalis, tobacco, tomato, other solanaceous plants. Information is given on the geographical distribution in ASIA, Bhutan, Burma, Ceylon, China, Christmas Island, Cocos-Keeling Islands, India, Indonesia, Japan, Korea, Laos, Malaysia, Pakistan, Philippine Islands, Sikkim, Singapore, Taiwan, Thailand, Vietnam, AFRICA, Aldabra Islands, Angola, Cameroun, Central African Republic, Comoro Islands, Congo, Gambia, Ghana, Ivory Coast, Kenya, Liberia, Malawi, Mali, Nigeria, Rhodesia, Senegal, Sierra Leone, South Africa, Tanzania, Uganda, AUSTRALASIA and PACIFIC ISLANDS, Australia, Fiji, Gambier Islands, Mariana Islands, Papua & New Guinea, Samoa, Society Islands, Solomon Islands, Tubuai, Irian Jaya.


2011 ◽  
Vol 50 (No. 2) ◽  
pp. 70-74 ◽  
Author(s):  
P. Sedlák ◽  
M. Melounová ◽  
S. Skupinová ◽  
P. Vejl ◽  
J. Domkářová

Potato cyst nematodes (PCN) are the big problem in worldwide planting of potatoes and another Solanaceous plants. Identification of individual pathotypes according to international scheme is very demanding but a very important part of the phytosanitary process to control these pests. Molecular genetic identification of different plant and animal species or individuals is a very interesting way at the present time and let’s hope that it will be important in future. This report presents results of the RAPD study of nine different real PCN populations. There were five Globodera rostochiensis populations and four G. pallida populations. Pathotypes Ro2, Ro2/3, Ro4, Ro5, Pa2 and Pa3 were from European populations; population Ro1 and X were of Czech provenance. Genetics variable of these populations was described by a set of six decameric primers (OPA 07, OPG 03, OPG 05, OPG 08, OPG 10 and OPG 13). Genetic dissimilarity was by Gel Manager for Windows evaluated. Detectable differences behind all populations were found and the dendrogram was compiled. The unknown population X was sorted into group of Globodera pallida species subgroup of Pa2 consequently.


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