Effect of different inocula of Meloidogyne incognita and Pseudomonas syringae pv. pisi with and without Rhizobium leguminosarum on growth, chlorophyll, carotenoid and proline contents of pea

2020 ◽  
Vol 73 (3) ◽  
pp. 499-506
Author(s):  
Deeksha Kashyap ◽  
Zaki A. Siddiqui
Keyword(s):  
Pseudomonas Syringae ◽  
Meloidogyne Incognita ◽  
Rhizobium Leguminosarum

Effects of Meloidogyne incognita, Pseudomonas syringae pv. pisi and Rhizobium leguminosarum inoculated alone, simultaneously, and sequentially, on the growth and biochemical parameters of pea (Pisum sativum) in three soil types

2021 ◽  
Author(s):  
Deeksha Kashyap ◽  
Zaki Anwar Siddiqui
Keyword(s):  
Fly Ash ◽  
Pisum Sativum ◽  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Meloidogyne Incognita ◽  
Biochemical Parameters ◽  
Rhizobium Leguminosarum ◽  
Soil Types ◽  
Carotenoid Content ◽  
Loam Soil

Abstract Effects of Meloidogyne incognita, Pseudomonas syringae pv. pisi and Rhizobium leguminosarum were studied on growth and biochemical parameters of pea (Pisum sativum L.) in three soil types. Plants grown in 20% fly ash attained higher plant growth, chlorophyll and carotenoid followed by loam soil and 20% sand. Inoculation of R. leguminosarum resulted in increased plant growth, nodulation, chlorophyll and carotenoid over control. Root nodulation and proline contents were high in plants grown in 20% sand and least in 20% fly ash. Inoculation of M. incognita prior to P. syringae pv. pisi resulted in a greater reduction in plant growth, nodulation, chlorophyll and carotenoid content and least where P. syringae pv. pisi was inoculated prior to M. incognita. Inoculation of pathogens increased proline contents. Galling and population of M. incognita was high in 20% sand followed by loam soil and fly ash amended soil. P. syringae pv. pisi and R. leguminosarum had adverse effect on galling and nematode population. The principal component analysis identifies interaction of pathogens and showed segregation of various treatments in the plots.

scholarly journals EFFICACY OF RHIZOSPHERIC ORGANISM Rhizobium leguminosarum AGAINST Meloidogyne incognita IN SOYBEAN

2016 ◽  
Vol 53 (02) ◽  
pp. 377-381
Author(s):  
Abdul Qudeer Ahmad ◽  
Nazir Javed ◽  
Sajid Aleem Khan ◽  
Huma Abbas ◽  
Muhammad Kamran
Keyword(s):  
Meloidogyne Incognita ◽  
Rhizobium Leguminosarum

scholarly journals Formation of phthalates during the degradation of N-phenyl-2-naphthylamine by soil bacteria

2021 ◽  
Vol 11 (1) ◽  
pp. 107-115
Author(s):  
L. E. Makarova ◽  
A. S. Morits ◽  
N. A. Sokolova
Keyword(s):  
Ethyl Acetate ◽  
Pseudomonas Syringae ◽  
Soil Bacteria ◽  
High Performance ◽  
Rhizobium Leguminosarum ◽  
Culture Media ◽  
Degradation Products ◽  
Bacterial Species ◽  
Gas Chromatography Mass Spectrometry ◽  
Negative Effect

N-phenyl-2-naphthylamine (N-PNA) and phthalates are classified as antibiotic substances. The appearance and accumulation of these substances in the biosphere is associated with their technogenic and biogenic origin (metabolites of plants and bacteria). In this article, we compare the degrading action of such soil bacteria as Rhizobium leguminosarum bv. viceae, Bradyrhizobium japonicum, Pseudomonas syringae pv. pisi, Clavibacter michiganensis sps. Sepedonicus and Azotobacter chroococcum against N-PNA. These bacteria differ in their interaction with pea plants (Pisum sativum L.) synthesising N-PNA. The degradation products were studied using gas chromatography-mass spectrometry in ethyl acetate extracts obtained from culture liquid media, in which N-PNA at a concentration of 10 μM and the bacteria under study were introduced. The decrease in the N-PNA concentration in the extracts obtained using ethyl acetate from culture media, in which N-PNA had been added to a concentration of 100 μM, was monitored following two days of bacterial growth using the methods of high-performance liquid chromatography. It was shown that all the studied bacterial species are capable of degrading N-PNA with the formation of phthalates. The Rhizobium bacteria, endosymbionts of pea plants synthesising N-PNA, and free-living nitrogen-fixing bacteria of the Azotobacter genus showed the highest degrading activity. It was found that N-PNA reduced the viability of all types of bacteria, although to a varying degree. N-PNA had the most negative effect on the viability of the Azotobacter genus, although these bacteria showed a high degrading action against N-PNA. The dependence between the negative effect of NPNA on bacterial viability and the N-PNA concentration was mildly pronounced for Rhizobium and Pseudomonas, although being significant for Bradyrhizobium and Clavibacter.

scholarly journals The Nif3-Family Protein YqfO03 from Pseudomonas syringae MB03 Has Multiple Nematicidal Activities against Caenorhabditis elegans and Meloidogyne incognita

2018 ◽  
Vol 19 (12) ◽  
pp. 3915 ◽  
Author(s):  
Abdul Manan ◽  
Zahoor Bazai ◽  
Jin Fan ◽  
Huafu Yu ◽  
Lin Li
Keyword(s):  
Caenorhabditis Elegans ◽  
Pseudomonas Syringae ◽  
Virulence Factors ◽  
Meloidogyne Incognita ◽  
C Elegans ◽  
Physical Defense ◽  
Family Protein ◽  
Important Pest ◽  
Protein Discovery ◽  
Common Plant

The nematicidal activity of the common plant-pathogenic bacterium Pseudomonas syringae against certain nematodes has been recently identified, but little is known about its virulence factors. In the current study, predictive analysis of nematode-virulent factors in the genome of a P. syringae wild-type strain MB03 revealed a variety of factors with the potential to be pathogenic against nematodes. One of these virulence factors that was predicted with a high score, namely, YqfO03, was a protein with structural domains that are similar to the Nif3 superfamily. This protein was expressed and purified in Escherichia coli, and was investigated for nematicidal properties against the model nematode Caenorhabditis elegans and an agriculturally important pest Meloidogyne incognita. Our results showed that YqfO03 exhibits lethal activity toward C. elegans and M. incognita worms, and it also caused detrimental effects on the growth, brood size, and motility of C. elegans worms. However, C. elegans worms were able to defend themselves against YqfO03 via a physical defense response by avoiding contact with the protein. Discovery of the diverse nematicidal activities of YqfO03 provides new knowledge on the biological function of a bacterial Nif3-family protein and insight into the potential of this protein as a specific means of controlling agricultural nematode pests.

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