scholarly journals Fatty acid composition, antibacterial and antioxidant potential of Atropa belladonna and Thymus linearis seeds grown in Kashmir

2021 ◽  
Vol 3 (7) ◽  
Author(s):  
Mohammad Azeem ◽  
Zubair Rehman Nengroo ◽  
M. O. Ahamad Khan ◽  
Adil Shafi Ganie

AbstractIn this study fatty acid composition of petroleum ether (AP and TP) and biological potential (Antioxidant and antibacterial) of chloroform (AC and TC), methanol (AM and TM) extracts of Atropa belladonna L. and Thymus linearis Benth. respectively was obtained by Soxhlet extraction technique from seeds were investigated. Fatty acid profile was obtained by gas chromatography mass spectrometry, antioxidant potential (DPPH-2,2-di-phenyl-1-picrylhydrazyl; ABTS-2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and antibacterial activity against (Pseudomonas aeruginosa, Serratia marcescens, Escherichia coli and Klebesiella pneumonia) was also performed. Linoleic acid was dominantly found with 74.42% in AP and 84.39% in TP. In antioxidant assays, the dominant inhibition was shown by AM (84.98% and 83.90%) and TM (85.27% and 83.29%) as compared to BHT (93.72% and 90.87%) for DPPH and ABTS radical scavenging respectively at 200 µg/mL. Moreover, AM and TM extracts showed good antibacterial activity. In conclusion, these plants could be selected as an adequate species in agricultural system, in food and pharmaceutical industries. Graphic abstract

Author(s):  
Zhuowei Li ◽  
Shijie Ma ◽  
Huan Song ◽  
Zheng Yang ◽  
Cuizhu Zhao ◽  
...  

Abstract Nervonic acid (24:1) is a major component in nerve and brain tissues and it has important applications in food and pharmaceutical industries. Malania oleifera seeds contain about 40% nervonic acid. However, the mechanism of nervonic acid biosynthesis and accumulation in seeds of this endangered tree species remains unknown. In this study, developmental changes in fatty acid composition within embryos and their pericarps were investigated. Nervonic acid proportions steadily increased in developing embryos but 24:1 was not detected in pericarps at any stage. Two 3-ketoacyl-CoA synthase (KCS) homologs have been isolated from M. oleifera developing seeds by homologous cloning methods. Both KCSs are expressed in developing embryos but not detected in pericarps. Based on a phylogenetic analysis, these two KCSs were named as MoKCS4 and MoKCS11. Seed-specific expression of the MoKCS11 in Arabidopsis thaliana led to about 5% nervonic acid accumulation, while expression of the MoKCS4 did not show an obvious change in fatty acid composition. It is noteworthy that the transformation of the same MoKCS11 construct into two Brassica napus cultivars with high erucic acid did not produce the expected accumulation of nervonic acid, although expression of MoKCS11 was detected in the developing embryos of transgenic lines. In contrast, overexpression of MoKCS11 results in similar level of nervonic acid accumulation in camelina, a species which contains a similar level of 11Z-eicosenoic acid as does Arabidopsis thaliana. Taken together, the MoKCS11 may have a substrate preference for 11Z-eicosenoic acid, but not for erucic acid, in planta.


2010 ◽  
Vol 46 (1) ◽  
pp. 95-96 ◽  
Author(s):  
N. Z. Mamadalieva ◽  
N. T. Ul’chenko ◽  
N. K. Yuldasheva ◽  
D. R. Egamberdieva ◽  
A. A. Zhanibekov ◽  
...  

2021 ◽  
Vol 72 (1) ◽  
pp. e393
Author(s):  
Z.R. Nengroo ◽  
A. Rauf

Angelica glauca Edgew. and Chenopodium album Linn. are medicinally important plants with aromatic, nutritious and flavor-enhancing properties. In this study the fatty acid composition of petroleum ether seed extracts (PE) of processed plants were analyzed by gas chromatography-mass spectrometry (GC-MS). The PE consisted mainly of unsaturated fatty acids, such as petroselinic acid 74.26% and oleic acid 7.37% for A. glauca and linoleic acid 53.05% and oleic acid 20.74% for C. album. The de-fatted seed extracts were screened for their antioxidant activities using 2,2-di-phenyl-1-picrylhydrazyl (DPPH), nitroblue tetrazolium (NBT), hydrogen peroxide (H2O2) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. Almost all the assays resulted in defatted seed extracts showing powerful radical scavenging activity. These findings suggest that the processed plants could be used as ingredients (as a source of natural antioxidants and unsaturated fatty acids) for the formulation of functional foods.


2004 ◽  
Vol 71 (1) ◽  
pp. 46-50 ◽  
Author(s):  
Dorthe Kristensen ◽  
Rikke V Hedegaard ◽  
Jacob H Nielsen ◽  
Leif H Skibsted

Milk from cows fed a low-fat diet high in cereals designed to stimulate fat synthesis de novo was lower in unsaturated fatty acids (21·3%) than milk from cows fed a diet high in fat, mainly from roasted soy beans (41·3% unsaturated fatty acids). Buttermilk from the more unsaturated milk was less oxidatively stable during storage (at 4 °C, followed for 11 d) than buttermilk from the more saturated milk, as monitored both by primary lipid oxidation products (lipid hydroperoxides) and by the secondary lipid oxidation product, hexanal. Fat-soluble antioxidants, β-carotene and α-tocopherol, analysed by HPLC, were not consumed during storage for either of the two types of buttermilk. In contrast, the antioxidative capacity of the serum phase decreased during storage as evaluated in a radical scavenging assay based on the semi-stable water-soluble radical nitrosodisulphonate (Fremy's salt). The time course for the decrease in water-soluble antioxidants was very similar for the two types of buttermilk suggesting that oxidation is initiated in the serum phase independently of fatty acid composition.


2013 ◽  
Vol 4 (1) ◽  
pp. 82-82
Author(s):  
Jessinta D/O Sandanasamy ◽  
Azhari H. Nour ◽  
Abdurahman H. Nour

2009 ◽  
Vol 74 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Pravat Mishra ◽  
Ayinampudi Sree

The fatty acid composition as well as the volatiles and an antibacterial screening of the total lipids isolated from marine sponge Phycopsis sp. collected at two different locations from the Bay of Bengal of the Orissa coast having different morphological features were studied. The content of linear saturated acids was 30.25 % in Phycopsis sp. 1, while their content reached 50.33 % in Phycopsis sp. 2. The amount of monobranched, saturated acids was 44.87 % in Phycopsis sp.1 and 38.83 % in Phycopsis sp. 2. There was more phytanic acid (7.92 %) in Phycopsis sp. 2 than in Phycopsis sp. 1 (4.06 %). The amount of 5,9-pentacosadienoic acid was found to be 5.54 % in Phycopsis sp. 1, while it was absent in Phycopsis sp. 2. Both species showed differences in their fatty acid composition and volatiles as well as in the antibacterial screening of their lipid extracts.


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