scholarly journals First reported case of Gilbertella persicaria in human stool: outcome of a community study from Segamat, Johor, Malaysia

2020 ◽  
Vol 51 (4) ◽  
pp. 2067-2075
Author(s):  
Marie Andrea Laetitia Huët ◽  
Li Wen Wong ◽  
Calvin Bok Sun Goh ◽  
Kuan Shion Ong ◽  
Jacky Dwiyanto ◽  
...  
Keyword(s):  
Community Study ◽  
Gilbertella Persicaria ◽  
Human Stool

Hepatitis A Antigen in Liver, Bile and Stool

1975 ◽  
Vol 33 ◽  
pp. 340-341
Author(s):  
D.R. Jackson ◽  
J.H. Hoofnagle ◽  
A.N. Schulman ◽  
J.L. Dienstag ◽  
R.H. Purcell ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Liver Enzymes ◽  
Hepatitis A Virus ◽  
Type A ◽  
Initial Study ◽  
Virus Like Particle ◽  
Elevated Liver Enzymes ◽  
Ag Particles ◽  
Ag Particle ◽  
Human Stool

Using immune electron microscopy Feinstone et. al. demonstrated the presence of a 27 nm virus-like particle in acute-phase stools of patients with viral hepatitis, type A, These hepatitis A antigen (HA Ag) particles were aggregated by convalescent serum from patients with type A hepatitis but not by pre-infection serum. Subsequently Dienstag et. al. and Maynard et. al. produced acute hepatitis in chimpanzees by inoculation with human stool containing HA Ag. During the early acute disease, virus like particles antigenically, morphologically and biophysically identical to the human HA Ag particle were found in chimpanzee stool. Recently Hilleman et. al. have described similar particles in liver and serum of marmosets infected with hepatitis A virus (HAV). We have investigated liver, bile and stool from chimpanzees and marmosets experimentally infected with HAV. In an initial study, a chimpanzee (no.785) inoculated with HA Ag-containing stool developed elevated liver enzymes 21 days after exposure.

scholarly journals Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 188
Author(s):  
Tanja Hoffmann ◽  
Andreas Hahn ◽  
Jaco J. Verweij ◽  
Gérard Leboulle ◽  
Olfert Landt ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Pcr Assay ◽  
Bead Beating ◽  
Stool Samples ◽  
Pcr Assays ◽  
Human Stool

This study aimed to assess standard and harsher nucleic acid extraction schemes for diagnostic helminth real-time PCR approaches from stool samples. A standard procedure for nucleic acid extraction from stool and a procedure including bead-beating as well as proteinase K digestion were compared with group-, genus-, and species-specific real-time PCR assays targeting helminths and nonhelminth pathogens in human stool samples. From 25 different in-house and commercial helminth real-time PCR assays applied to 77 stool samples comprising 67 historic samples and 10 external quality assessment scheme samples positively tested for helminths, higher numbers of positive test results were observed after bead-beating-based nucleic acid extraction for 5/25 (20%) real-time PCR assays irrespective of specificity issues. Lower cycle threshold values were observed for one real-time PCR assay after the standard extraction scheme, and for four assays after the bead-beating-based scheme. Agreement between real-time PCR results after both nucleic acid extraction strategies according to Cohen’s kappa ranged from poor to almost perfect for the different assays. Varying agreement was observed in eight nonhelminth real-time PCR assays applied to 67 historic stool samples. The study indicates highly variable effects of harsh nucleic acid extraction approaches depending on the real-time PCR assay used.

scholarly journals A method for detection of SARS-CoV-2 RNA in healthy human stool: a validation study

2021 ◽  
Author(s):  
Michael P Coryell ◽  
Mikhail Iakiviak ◽  
Nicole Pereira ◽  
Pallavi P Murugkar ◽  
Jason Rippe ◽  
...  
Keyword(s):  
Validation Study ◽  
Healthy Human ◽  
Human Stool

EXPRESSION ANALYSIS OF THE NEP-1 AND CELL-WALL DEGRADING GENES OF Gilbertella persicaria DURING PATHOGENESIS IN PAPAYA (Carica papaya L.) FRUITS

2021 ◽  
pp. 101676
Author(s):  
Isabel Cruz-Lachica ◽  
Isidro Márquez-Zequera ◽  
Raúl Allende-Molar ◽  
Josefina León-Félix ◽  
Josefa Adriana Sañudo-Barajas ◽  
...  
Keyword(s):  
Cell Wall ◽  
Expression Analysis ◽  
Carica Papaya ◽  
Gilbertella Persicaria
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