scholarly journals Influence of the endocannabinoid system on the antidepressant activity of bupropion and moclobemide in the behavioural tests in mice

2020 ◽  
Vol 72 (6) ◽  
pp. 1562-1572
Author(s):  
Ewa Poleszak ◽  
Sylwia Wośko ◽  
Karolina Sławińska ◽  
Elżbieta Wyska ◽  
Aleksandra Szopa ◽  
...  
Keyword(s):  
Forced Swim Test ◽  
Antidepressant Drugs ◽  
Endocannabinoid System ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Cellular Level ◽  
Hplc Method ◽  
Receptor Function ◽  
Receptor Ligands ◽  
Behavioural Tests

Abstract Background Though there are several classes of antidepressant drugs available on the pharmaceutical market, depression that affects globally over 320 million people is still undertreated. Scientists have made attempts to develop novel therapeutical strategies to maximize effectiveness of therapy and minimize undesired reactions. One of the ideas is use of either dual-action agents or combined administration of two substances that affect diverse neurotransmissions. Thus, we investigated whether the selected CB receptor ligands (oleamide, AM251, JWH133, and AM630) can have an impact on the activity of bupropion and moclobemide. Bupropion belongs to the dual acting drugs, whereas moclobemide is an inhibitor of monoamine oxidase. Methods The mice forced swim test and the tail suspension test were applied in order to determine the potential antidepressant-like activity, whereas the HPLC method was used in order to assess the brain concentrations of the tested antidepressants. Results An intraperitoneal injection of sub-effective doses of oleamide (5 mg/kg), AM251 (0.25 mg/kg), and AM630 (0.25 mg/kg) increased activity of bupropion (10 mg/kg) in both behavioural tests. Effects of moclobemide (1.5 mg/kg) were potentiated only by AM251. These results were not influenced by the hypo- or hyperlocomotion of animals. Conclusion The outcomes of the present study revealed that particularly activation or inhibition of the CB1 receptor function may augment the antidepressant activity of bupropion, whereas only inhibition of the CB1 receptor function manages to increase activity of moclobemide. Most probably, an interplay between CB receptor ligands and bupropion or moclobemide takes place at the cellular level.

scholarly journals Anti-Depressant Activity of the Leaves of Zanthoxylum Armatum on Swiss Albino Mice

2020 ◽  
Vol 8 (02) ◽  
pp. 46-50
Author(s):  
Chandrajeet Kumar Yadav ◽  
Kamal Poudel ◽  
Roshan Mehta ◽  
Amit Kumar Shrivastava
Keyword(s):  
Methanolic Extract ◽  
Forced Swim Test ◽  
Antidepressant Drugs ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Medical Problem ◽  
Mild Stress ◽  
Swiss Albino Mice ◽  
Zanthoxylum Armatum ◽  
Albino Mice

INTRODUCTION Depression is a global mental disorder that has high incidence, high recurrence, and high self-mutilation and suicide rates. Although the antidepressant drugs are available for the treatment, depression still continues to be a major medical problem. The present study was designed to study the anti-depressant activity of the leaves extract of Zanthoxylum armatum using forced swim test and tail suspension test (TST) on Swiss albino mice. MATERIAL AND METHODS The anti-depressant activity of the leaves of Zanthoxylum armatum was assessed using chronic unpredictable mild-stress (CUMS) induced depression in mice. The animals were treated with the methanolic extract of leaves of Zanthoxylum armatum orally at two doses of 100, 200 mg/kg body weight for eight days after (CUMS) induced depression in mice. RESULTS The data were analyzed by one-way ANOVA followed by tukey multiple comparison test. The leaves extract presented significant antidepressant activity in mice (p<0.05), CONCLUSION The results demonstrate that methanolic extract of leaves of Zanthoxylum armatum has got significant antidepressant activity.

scholarly journals Design, Synthesis, and In Vivo and In Silico Evaluation of Coumarin Derivatives with Potential Antidepressant Effects

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5556
Author(s):  
Xuekun Wang ◽  
Hao Zhou ◽  
Xinyu Wang ◽  
Kang Lei ◽  
Shiben Wang
Keyword(s):  
Molecular Docking ◽  
High Resolution Mass Spectrometry ◽  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Homology Model ◽  
Coumarin Derivatives ◽  
Monitoring Methods ◽  
Discovery Studio

In this study, a series of coumarin derivatives were designed and synthesized, their structures were characterized using nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS) testing methods. In the pharmacological experiment, two behavior-monitoring methods, the forced swim test (FST) and the tail suspension test (TST), were used to determine the antidepressant activity of coumarin derivatives. Compounds that showed potential activity were analyzed for their effects on 5-hydroxytryptamine (5-HT) levels in the brains of mice. Molecular docking experiments to simulate the possible interaction of these compounds with the 5-HT1A receptor was also be predicted. The results of the pharmacological experiments showed that most coumarin derivatives exhibited significant antidepressant activity. Among these compounds, 7-(2-(4-(4-fluorobenzyl)piperazin-1-yl)-2-oxoethoxy)-2H-chromen-2-one (6i) showed the highest antidepressant activity. The results of the measurement of 5-HT levels in the brains of mice indicate that the antidepressant activity of coumarin derivatives may be mediated by elevated 5-HT levels. The results of molecular docking demonstrated that compound 6i had a significant interaction with amino acids around the active site of the 5-HT1A receptor in the homology model. The physicochemical and pharmacokinetic properties of the target compounds were also predicted using Discovery Studio (DS) 2020 and Chemdraw 14.0.

scholarly journals Evaluation of antidepressant activity of tramadol in comparison with imipramine in Swiss albino mice

2017 ◽  
Vol 6 (3) ◽  
pp. 695
Author(s):  
Chiranjeevi Bonda ◽  
Sudhir Pawar ◽  
Jaisen Lokhande
Keyword(s):  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Antidepressant Effect ◽  
Tail Suspension ◽  
Swim Test ◽  
Forced Swim ◽  
Group I ◽  
Immobility Time ◽  
Suspension Test

Background: The aim of the study was to evaluate the antidepressant effect of opioid analgesic tramadol using forced swim test and tail suspension test models.Methods: The antidepressant effect was assessed by recording the immobility time in Forced swim test (FST) and Tail suspension test (TST). The mice were randomly divided into five groups. Mice belonging to group I was given normal saline (0.1ml/kg) which acted as control. Group II received imipramine (15mg/kg) considered as the standard drug tramadol was given in graded dose (10, 20 and 40 mg/kg) to mice of groups III, IV, V respectively. All drugs were administered intraperitoneally for seven successive days; test was done on 7th day.Results: Tramadol and Imipramine showed antidepressant activity when compared to control. There is dose dependent increase in antidepressant activity of tramadol. The antidepressant activity of imipramine was significantly (P<0.05) more than tramadol at dose 10 and 20 mg/kg but antidepressant activity with tramadol 40mg/kg was comparable to imipramine treated mice.Conclusions: The results of this study indicated the presence of antidepressant activity of tramadol at 40mg/kg.

scholarly journals Antidepressant-Like Activity of the Ethanolic Extract fromUncaria lanosaWallich var.appendiculataRidsd in the Forced Swimming Test and in the Tail Suspension Test in Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Lieh-Ching Hsu ◽  
Yu-Jen Ko ◽  
Hao-Yuan Cheng ◽  
Ching-Wen Chang ◽  
Yu-Chin Lin ◽  
...  
Keyword(s):  
Forced Swimming Test ◽  
Antidepressant Drugs ◽  
Ethanolic Extract ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Probable Mechanism ◽  
Tail Suspension ◽  
Swimming Test ◽  
Mice Brain ◽  
Suspension Test

This study investigated the antidepressant activity of ethanolic extract ofU. lanosaWallich var.appendiculataRidsd (ULEtOH) for two-weeks administrations by using FST and TST on mice. In order to understand the probable mechanism of antidepressant-like activity of ULEtOHin FST and TST, the researchers measured the levels of monoamines and monoamine oxidase activities in mice brain, and combined the antidepressant drugs (fluoxetine, imipramine, maprotiline, clorgyline, bupropion and ketanserin). Lastly, the researchers analyzed the content of RHY in the ULEtOH. The results showed that ULEtOHexhibited antidepressant-like activity in FST and TST in mice. ULEtOHincreased the levels of 5-HT and 5-HIAA in cortex, striatum, hippocampus, and hypothalamus, the levels of NE and MHPG in cortex and hippocampus, the level of NE in striatum, and the level of DOPAC in striatum. Two-week injection of IMI, CLO, FLU and KET enhanced the antidepressant-like activity of ULEtOH. ULEtOHinhibited the activity of MAO-A. The amount of RHY in ULEtOHwas 17.12 mg/g extract. Our findings support the view that ULEtOHexerts antidepressant-like activity. The antidepressant-like mechanism of ULEtOHmay be related to the increase in monoamines levels in the hippocampus, cortex, striatum, and hypothalamus of mice.

scholarly journals EFFECT OF FLAVONE AND ITS MONOHYDROXY DERIVATIVES ON ANIMAL MODELS OF DEPRESSION IN SWISS ALBINO MICE

2019 ◽  
pp. 513-517
Author(s):  
SHANMUGAPRIYAN S ◽  
JAIKUMAR S ◽  
VISWANATHAN S ◽  
PARIMALA K ◽  
RAJESH M
Keyword(s):  
Animal Models ◽  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Tail Suspension ◽  
Swim Test ◽  
Forced Swim ◽  
Animal Models Of Depression ◽  
Pre Treatment ◽  
Suspension Test

Objectives: This research was designed to investigate the antidepressant activity of a few structurally related flavones (flavone, 3‑hydroxyflavone, and 7‑hydroxyflavone) and the possible mechanisms involved. Methods: Antidepressant activity was evaluated in mice by subjecting them to forced swim test and tail suspension test. The involvement of adrenergic, serotonergic, nitric oxide (NO), and opioid mechanisms was investigated using suitable interacting chemicals. Results: Flavone, 3‑hydroxyflavone, and 7‑hydroxyflavone exhibited a significant and dose‑dependent reduction in total time of immobility in the forced swim test and tail suspension test. Pre‑treatment with alpha‑methyl‑para‑tyrosine and parachlorophenyl alanine attenuated the reduction in immobility period produced by flavone and its derivatives in forced swim test. Naloxone pre‑treatment partially reversed the effect of flavone while L‑arginine pre‑treatment did not alter their effect. Conclusion: The investigated flavones exhibited promising antidepressant activity in both the animal models of depression. However, the flavone compounds did not alter the motor coordination and ambulatory behavior in the Rotarod and locomotor activity test. The participation of serotonergic, adrenergic, and opioid mechanism in the antidepressant activity of these compounds was elucidated from the results, and the role of NO pathway was excluded.

scholarly journals A comparative study of the antidepressant effect of ondansetron and granisetron on Albino mice

2017 ◽  
Vol 6 (12) ◽  
pp. 2922
Author(s):  
Mansi J. Shah ◽  
Geetha M. ◽  
Rahul H. D. ◽  
Shashikala G. H.
Keyword(s):  
Animal Studies ◽  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Experimental Models ◽  
Antidepressant Effect ◽  
Test Model ◽  
Potential Candidate ◽  
Test Drug ◽  
Standard Drug

Background: Ondansetron and granisetron are selective 5-HT receptor antagonists used as antiemetics. The present study as aimed at comparing the antidepressant activity of ondansetron and granisetron in animal experimental models.Methods: The study was done after obtaining approval from the institutional animal ethical committee of JJM Medical college, Davangere and CPCSEA. A total of 24 mice of either sex and of weight between 20-40g were included in the study. The antidepressant activity of ondansetron and granisetron was evaluated in mice using forced swim test model (FST) and tail suspension test model (TST). In both the experimental models animals were divided into 4 groups and received the following drugs- Group 1 (control) - normal saline 10mg/kg i.p, Group 2 (standard)-fluoxetine 10mg/kg i.p, Group 3(test drug 1), ondansetron 2mg/kg i.p, Group 4 (test drug 2)- granisetron 0.5mg/kg i.p. The duration of immobility was noted and compared amongst the 4 groups in both the models 60 min after drug administration. The observations were analysed using ANOVA (one way) and post hoc Tukey’s test.Results: The test drugs showed significant reduction in duration of immobility in both the models. In FST and TST models, granisetron (0.5mg/kg i.p) showed a significant reduction in immobility period of 10.33 sec and 67 secs respectively when compared to ondansetron (2mg/kg i.p) and the standard drug fluoxetine (10mg/kg i.p).Conclusions: The results of study suggest that granisetron may be useful as a potential candidate for treatment of depression. Hence further animal studies with different model for depression and clinical studies should be conducted in order to choose the better drug for treatment PONV which is often associated with depression.

Synthesis and Antidepressant activity of pyrazoline based MAO-inhibitors

2016 ◽  
Vol 3 (1) ◽  
pp. 1 ◽  
Author(s):  
Vishnu Nayak Badavath ◽  
Alok Kumar ◽  
Surender Singh Jadav ◽  
Ashok Kumar Pattnaik ◽  
Venkatesan Jayaprakash ◽  
...  
Keyword(s):  
Elevated Plus Maze ◽  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Mao Inhibitors ◽  
Tail Suspension ◽  
Swim Test ◽  
Plus Maze ◽  
Behavioral Despair Test ◽  
Suspension Test

A series of nine 3-(2-hydroxyphenyl)-5-aryl-N-phenyl-4,5-dihydropyrazole-1-carbothioamide derivative s (3a-3i) that were earlier reported as potent rMAO-A inhibitors were evaluated for their antidepressant activity in Porsolt's behavioral despair test (forced swim test) and Tail Suspension test activity, among them, compounds (3e and 3h) were found to have potent antidepressant activity. Reduction in duration of immobility was significant for all the compounds in Porsolts swim test compared with tail suspension test. 3h was further evaluated for anxiolytic activity in Elevated plus maze and was found to be devoid of it.

scholarly journals PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF COMMIPHORA MUKUL FOR ANTIDEPRESSANT ACTIVITY IN ALBINO MICE

2016 ◽  
Vol 10 (1) ◽  
pp. 360
Author(s):  
Vijetha Pendyala ◽  
Ramesh Babu Janga ◽  
Vidyadhara Suryadevara
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Phytochemical Screening ◽  
Tail Suspension ◽  
Swim Test ◽  
Commiphora Mukul ◽  
Suspension Test

ABSTRACTObjective: The main aim and objective of the present study is to investigate the effect of Commiphora mukul (Family: Burseraceae), on depression inmice using tail suspension test (TST) and forced swim test (FST).Methods: The oleo-gum resin of guggul was extracted with alcohol and fractionated with ethyl acetate and petroleum ether. All the fractions weresubjected for preliminary phytochemical screening, using various qualitative tests. Till date, no scientific data were available on the antidepressantactivity of this plant. So, in the present investigation, TST and FST are selected as animal models for evaluation of antidepressant activity in albinomice.Results: The preliminary phytochemical screening of guggul has revealed the presence of carbohydrates, proteins, tannins, and flavonoids inhydroalcoholic fraction. Ethyl acetate fraction showed positive results toward flavonoids, alkaloids, proteins, and steroids. Hydroalcoholic, ethylacetate, and petroleum ether fractions (50 and 100 mg/kg p.o.) of guggul administered orally for 14 successive days had decreased the immobilityperiods significantly in a dose-dependent manner in both TST and FST, showing significant antidepressant-like activity. The activities of the fractionswere found to be comparable to imipramine in both FST and TST.Conclusions: Although a number of synthetic drugs are being used as standard treatment for clinically depressed patient, they have adverse effectsthat can compromise the therapeutic treatment. In the traditional systems of medicine, many plants and formulations have been used to treatdepression for thousands of years. The results of this study indicate the potential for the use of guggul as an adjuvant in the treatment of depression.Keywords: Guggul, Commiphora mukul, Antidepressant activity, Forced swim test, Tail suspension test, Depression.

scholarly journals Tail suspension test to evaluate the antidepressant activity of experimental drugs

2016 ◽  
Vol 11 (2) ◽  
pp. 292 ◽  
Author(s):  
Muhammad Aslam
Keyword(s):  
Forced Swimming Test ◽  
Antidepressant Drugs ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Tail Suspension ◽  
Video Clips ◽  
Experimental Drugs ◽  
Behavioral Paradigm ◽  
Swimming Test ◽  
Suspension Test

<p>Tail suspension test (TST) is a common behavioral paradigm used to evaluate the antidepressant activity of experimental drugs. Like the forced swimming test, in this test the animals are set in an inescapable yet modestly distressing circumstance. In the tail suspension test, the extent of immobility is thought to be associated with the depressive-like condition of the animal and is significantly diminished by antidepressant drugs.</p><p><strong>Video clips</strong></p><p><a href="https://youtube.com/v/OqWrALPAGhA">Pre-experiment</a>:         53 sec</p><p><a href="https://youtube.com/v/T7sRbrn3xbQ">Experiment</a>:      7 min 31 sec</p><p> </p>

Sign in / Sign up

Export Citation Format

Share Document