Glucose Induction

2012 ◽  
Keyword(s):  
Glucose Induction

scholarly journals Potentiality of Self-Cloned Lactobacillus plantarum Taj-Apis362 for Enhancing GABA Production in Yogurt under Glucose Induction: Optimization and Its Cardiovascular Effect on Spontaneous Hypertensive Rats

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1826
Author(s):  
Farah Salina Hussin ◽  
Shyan Yea Chay ◽  
Mohammad Zarei ◽  
Anis Shobirin Meor Hussin ◽  
Wan Zunairah Wan Ibadullah ◽  
...  
Keyword(s):  
Dairy Product ◽  
Starter Culture ◽  
Hypertensive Rats ◽  
Fermentation Time ◽  
Spontaneously Hypertensive ◽  
Gaba Production ◽  
Pressure Lowering ◽  
Gaba Content ◽  
Glucose Induction

The current study evaluated the γ-aminobutyric acid (GABA) producing ability from three novel strains of lactic acid bacteria (L. plantarum Taj-Apis362, assigned as UPMC90, UPMC91, and UPMC1065) co-cultured with starter culture in a yogurt. A combination of UPMC90 + UPMC91 with starter culture symbiotically revealed the most prominent GABA-producing effect. Response surface methodology revealed the optimized fermentation conditions at 39.0 °C, 7.25 h, and 11.5 mM glutamate substrate concentration to produce GABA-rich yogurt (29.96 mg/100 g) with desirable pH (3.93) and water-holding capacity (63.06%). At 2% glucose to replace pyridoxal-5-phosphate (PLP), a cofactor typically needed during GABA production, GABA content was further enhanced to 59.00 mg/100 g. In vivo study using this sample revealed a blood pressure-lowering efficacy at 0.1 mg/kg GABA dosage (equivalent to 30 mg/kg GABA-rich yogurt) in spontaneously hypertensive rats. An improved method to produce GABA-rich yogurt has been established, involving shorter fermentation time and lower glutamate concentration than previous work, along with glucose induction that omits the use of costly PLP, fostering the potential of developing a GABA-rich functional dairy product through natural fermentation with desirable product quality and antihypertensive property.

scholarly journals Regulatory Network Connecting Two Glucose Signal Transduction Pathways in Saccharomyces cerevisiae

2004 ◽  
Vol 3 (1) ◽  
pp. 221-231 ◽  
Author(s):  
Aneta Kaniak ◽  
Zhixiong Xue ◽  
Daniel Macool ◽  
Jeong-Ho Kim ◽  
Mark Johnston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Signal Transduction ◽  
Transcription Factor ◽  
Regulatory Network ◽  
Target Genes ◽  
Glucose Repression ◽  
Signal Transduction Pathways ◽  
Glucose Signaling ◽  
Genes Encoding ◽  
Glucose Induction

ABSTRACT The yeast Saccharomyces cerevisiae senses glucose, its preferred carbon source, through multiple signal transduction pathways. In one pathway, glucose represses the expression of many genes through the Mig1 transcriptional repressor, which is regulated by the Snf1 protein kinase. In another pathway, glucose induces the expression of HXT genes encoding glucose transporters through two glucose sensors on the cell surface that generate an intracellular signal that affects function of the Rgt1 transcription factor. We profiled the yeast transcriptome to determine the range of genes targeted by this second pathway. Candidate target genes were verified by testing for Rgt1 binding to their promoters by chromatin immunoprecipitation and by measuring the regulation of the expression of promoter lacZ fusions. Relatively few genes could be validated as targets of this pathway, suggesting that this pathway is primarily dedicated to regulating the expression of HXT genes. Among the genes regulated by this glucose signaling pathway are several genes involved in the glucose induction and glucose repression pathways. The Snf3/Rgt2-Rgt1 glucose induction pathway contributes to glucose repression by inducing the transcription of MIG2, which encodes a repressor of glucose-repressed genes, and regulates itself by inducing the expression of STD1, which encodes a regulator of the Rgt1 transcription factor. The Snf1-Mig1 glucose repression pathway contributes to glucose induction by repressing the expression of SNF3 and MTH1, which encodes another regulator of Rgt1, and also regulates itself by repressing the transcription of MIG1. Thus, these two glucose signaling pathways are intertwined in a regulatory network that serves to integrate the different glucose signals operating in these two pathways.

scholarly journals Effect of Glucose Induction on Biofilm Density in Clinical Isolate Acinetobacter baumannii Patients in Intensive Care Unit of Dr. Soetomo Hospital, Surabaya

2020 ◽  
Vol 56 (2) ◽  
pp. 118
Author(s):  
Wira W Lindarto ◽  
Eddy Bagus Wasito ◽  
Kartuti Debora
Keyword(s):  
Tissue Culture ◽  
Acinetobacter Baumannii ◽  
Clinical Isolate ◽  
Growth Media ◽  
Plate Method ◽  
Culture Plate ◽  
Tissue Culture Plate ◽  
Glucose Induction ◽  
Sugar Levels ◽  
Effect Of Glucose

This study aimed to analyze the effect of glucose induction on the clinical isolate biofilm density of Acinetobacter baumannii. Thirteen clinical isolates of A. baumannii non biofilm forming were collected from non-DM patients who were treated at the ICU of Dr. Soetomo Hospital, Surabaya, was treated with the addition of 0.08% glucose, 0.15% glucose, 0.2% glucose, and 0.4% glucose in TSB growth media, followed by biofilm density examination with Tissue Culture Plate Method (TCPM) using 96 wells flatbottomed polyesterene tissue culture plate and read by autoreader ELISA with a wavelength of 630 nm (OD630). Biofilm density obtained was analyzed using ANOVA statistical analysis. The results of OD630 showed that the biofilm density increased significantly at the addition of 0.2% and 0.4% glucose. There was a significant increase in biofilm density at the addition of 0.2% and 0.4% glucose so that the management of blood sugar levels in ICU patients was needed before and when medical devices were installed.

scholarly journals Glucose induction pathway regulates meiosis inSaccharomyces cerevisiaein part by controlling turnover of Ime2p meiotic kinase

2008 ◽  
Vol 8 (5) ◽  
pp. 676-684 ◽  
Author(s):  
Misa Gray ◽  
Sarah Piccirillo ◽  
Kedar Purnapatre ◽  
Brandt L. Schneider ◽  
Saul M. Honigberg
Keyword(s):  
Glucose Induction

Sustained Wnt/β-Catenin Signaling Rescues High Glucose Induction of Transforming Growth Factor-β1-Mediated Renal Fibrosis

2012 ◽  
Vol 344 (5) ◽  
pp. 374-382 ◽  
Author(s):  
Cheng Ho ◽  
Pei-Hsien Lee ◽  
Yung-Chien Hsu ◽  
Yu-Ting Huang ◽  
Feng-Sheng Wang ◽  
...  
Keyword(s):  
Growth Factor ◽  
High Glucose ◽  
Renal Fibrosis ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Glucose Induction

scholarly journals Evidence for involvement ofSaccharomyces cerevisiaeprotein kinase C in glucose induction ofHXTgenes and derepression ofSUC2

2002 ◽  
Vol 2 (2) ◽  
pp. 93-102 ◽  
Author(s):  
R.L. Brandão ◽  
L Etchebehere ◽  
C.C. Queiroz ◽  
M.J. Trópia ◽  
J.R. Ernandes ◽  
...  
Keyword(s):  
Kinase C ◽  
Glucose Induction

scholarly journals High glucose induction of DNA-binding activity of the transcription factor NFκB in patients with diabetic nephropathy

2008 ◽  
Vol 1782 (5) ◽  
pp. 295-302 ◽  
Author(s):  
Bingmei Yang ◽  
Andrea Hodgkinson ◽  
Peter J. Oates ◽  
Beverley A. Millward ◽  
Andrew G. Demaine
Keyword(s):  
Transcription Factor ◽  
Diabetic Nephropathy ◽  
Dna Binding ◽  
High Glucose ◽  
Binding Activity ◽  
Dna Binding Activity ◽  
Glucose Induction ◽  
Transcription Factor Nfκb

scholarly journals A glycolytic burst drives glucose induction of global histone acetylation by picNuA4 and SAGA

2009 ◽  
Vol 37 (12) ◽  
pp. 3969-3980 ◽  
Author(s):  
R. Magnus N. Friis ◽  
Bob P. Wu ◽  
Stacey N. Reinke ◽  
Darren J. Hockman ◽  
Brian D. Sykes ◽  
...  
Keyword(s):  
Histone Acetylation ◽  
Glucose Induction
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