The uptake of 2-14C-5-hydroxytryptamine by uterine tissue of nonpregnant and pregnant rats

1971 ◽  
Vol 110 (7) ◽  
pp. 919-923
Author(s):  
Zeev Koren ◽  
Eric Bloch
Keyword(s):  
Uterine Tissue ◽  
Pregnant Rats

METABOLISM OF 20β-HYDROXY-4-PREGNEN-3-ONE IN UTERINE TISSUE OF NON-PREGNANT RATS IN VITRO

1976 ◽  
Vol 83 (3) ◽  
pp. 604-620 ◽  
Author(s):  
B. P. Lisboa ◽  
M. Holtermann
Keyword(s):  
Biological Activity ◽  
Adult Female ◽  
Female Rats ◽  
Uterine Tissue ◽  
Rat Uterus ◽  
Adult Rats ◽  
Pregnant Rats ◽  
Progesterone Metabolites ◽  
Ovariectomized Adult Rats

ABSTRACT In vitro experiments carried out with uterus preparations of ovariectomized adult rats indicate the presence in this tissue of a 20β-hydroxysteroid-oxidoreductase which catalyzes the conversion of 20β-hydroxy-4-pregnen-3-one to progesterone. Since a hepatic 20β-hydroxysteroid-oxidoreductase is absent in adult female rats, the myometrial enzyme can be responsible for the biological activity of 20β-hydroxy-4-pregnen-3-one in these animals. Besides progesterone five metabolites were isolated and identified after incubation of [4-14C]20β-hydroxy-4-pregnen-3-one with uterine tissue: 20β-hydroxy-5α-pregnan-3-one, 20β-hydroxy-5β-pregnan-3-one, 5α-pregnane-3α,20β-diol, 4-pregnene-3α,20β-diol and 4-pregnene-3β,20β-diol. The conversion of 20β-hydroxy-4-pregnen-3-one to progesterone permits us to regard all five steroids isolated as progesterone metabolites in the rat uterus. 20β-hydroxy-5β-pregnan-3-one is the first C21-metabolite with a 5β(H)-configuration isolated in the rat uterus, which indicates the presence of 5β-reductase in this tissue.

METABOLISM OF PROGESTERONE IN UTERINE TISSUE OF NON-PREGNANT RATS IN VITRO

1976 ◽  
Vol 83 (3) ◽  
pp. 583-603 ◽  
Author(s):  
B. P. Lisboa ◽  
M. Holtermann
Keyword(s):  
Quantitative Difference ◽  
Total Activity ◽  
Allylic Alcohols ◽  
Uterine Tissue ◽  
Oxidoreductase Activity ◽  
Pregnant Rats ◽  
Progesterone Metabolites ◽  
Influence Of Substrate ◽  
First Time

ABSTRACT The metabolism of labelled progesterone was studied in vitro in uterine tissue of non-pregnant rats with particular emphasis on the influence of substrate concentration. Neither a qualitative nor quantitative difference was found for a steroid tissue ratio between 15 × 10−6 and 4.2 × 10−9 to 1 g (substrate amounts between 57.73 and 0.02 nmol); with both concentrations 42 to 44 per cent of progesterone was metabolized to about 35 per cent monohydroxymonoketonic steroids and 4–6 per cent dihydroxylated C21O2-compounds. In both sets of incubations we have isolated and identified the following steroids: 3α-hydroxy-5α-pregnan-20-one, 3β-hydroxy-4-pregnen-20-one, 3α-hydroxy-4-pregnen-20-one, 20α-hydroxy-4-pregnen-3-one, 5α-pregnane-3α,20α-diol and 4-pregnene-3α,20α-diol. The most abundant metabolite formed in these incubations was 3α-hydroxy-4-pregnen-20-one -which corresponds to about 30 per cent of the total activity recovered. It is the first time that the presence of 20α-hydroxysteroid-oxidoreductase activity is definitely established in this type of tissue. The identification of three allylic alcohols as progesterone metabolites in the rat uterus confirms that Δ4-3-hydroxysteroids are important intermediates in the in vitro uterine metabolism of steroids.

Progesterone metabolism by uterine tissue of pregnant rats

Steroids ◽  
1972 ◽  
Vol 19 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Phillenore D. Howard ◽  
Walter G. Wiest
Keyword(s):  
Uterine Tissue ◽  
Pregnant Rats ◽  
Progesterone Metabolism

scholarly journals The effects of the aromatase inhibitor fadrozole hydrochloride on fetuses and uteri in late pregnant rats

2004 ◽  
Vol 180 (2) ◽  
pp. 337-345 ◽  
Author(s):  
H Tamada ◽  
Y Shimizu ◽  
T Inaba ◽  
N Kawate ◽  
T Sawada
Keyword(s):  
Physical Properties ◽  
Aromatase Inhibitor ◽  
Mrna Expression ◽  
Lysyl Oxidase ◽  
Late Pregnancy ◽  
Estrogen Deficiency ◽  
Uterine Tissue ◽  
Pregnant Rats ◽  
Toxicological Effects ◽  
Matrix Metalloproteinase 1

It is well known that progesterone and estrogen are essential hormones for maintaining pregnancy in most mammals. Some specific roles of progesterone for the maintenance of pregnancy have been clarified, but the role of estrogen is not well known. This study examines the effects of the aromatase inhibitor, fadrozole hydrochloride (Fad), on fetuses, uterine physical properties and the mRNA expression of the uterine enzymes that are related to collagen metabolism during late pregnancy in rats. Continuous s.c. infusion with 300 micro g/day Fad from day 14 of pregnancy (day 1=the day of sperm detection) reduced the concentration of plasma estradiol-17beta (E(2)), and did not change that of plasma progesterone, compared with controls. The treatment increased the intrauterine pressure and reduced the size and compliance of the uterine tissue framework. It also caused injuries (hematomata on the extremities) in about one-quarter of fetuses by day 20. The collagen content of the uterine ampullae was not changed by the treatment. Uterine mRNA expressions of matrix metalloproteinase-1 (MMP-1), which degrades collagens, and of lysyl oxidase (LO), which is necessary for the formation of intra- and inter-molecular cross-links of collagen, were examined by quantitative RT-PCR. The treatment with Fad had no effect on the expression of MMP-1 mRNA and increased that of LO mRNA. Daily s.c. injection with 0.2 micro g E(2) restored the changes in uterine physical properties and the mRNA expression of LO caused by the Fad treatment, and prevented fetal injury, indicating that the influences of Fad treatment are due to estrogen deficiency but not to toxicological effects of Fad. These results imply that estrogen deficiency during late pregnancy in rats obstructs development of the uterine tissue framework so as to cause fetal injury. It is possible that an increase in the uterine expression of LO gene may be involved in this obstruction.

PROGESTERONE IN THE UTERUS

1974 ◽  
Vol 77 (1) ◽  
pp. 160-170 ◽  
Author(s):  
D. Egert ◽  
H. Maass
Keyword(s):  
Extracellular Fluid ◽  
Uterine Tissue ◽  
Rat Uterus ◽  
Pregnant Rats ◽  
Pregnant Rat

ABSTRACT Radio-metabolites of progesterone and progesterone itself were found in the uteri of intact pregnant rats 20 min after injection of [1,2-3H]progesterone. However, after evisceration and removal of the foeto-placental unit, and following injection of the labelled steroid, the progesterone content in the extract from the pregnant rat uterus markedly increased while the metabolite levels correspondingly decreased. An analogous change was observed in the plasma. Thus it appears probable that progesterone is not metabolized in the uterus of pregnant rats, and that metabolites found in this tissue originate predominantly in the foetoplacental system and appear in the plasma and extracellular fluid present in the uterine tissue.

Modification of Pineal Ultrastructure by Exogenous Hormones

1967 ◽  
Vol 25 ◽  
pp. 170-171
Author(s):  
R. A. Turner ◽  
A. E. Rodin ◽  
D. K. Roberts
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Estradiol Benzoate ◽  
Female Rats ◽  
List Type ◽  
Type Number ◽  
Pregnant Rats ◽  
Gonadal Atrophy ◽  
Pineal Ultrastructure

There have been many reports which establish a relationship between the pineal and sexual structures, including gonadal hypertrophy after pinealectomy, and gonadal atrophy after injection of pineal homogenates or of melatonin. In order to further delineate this relationship the pineals from 5 groups of female rats were studied by electron microscopy:ControlsPregnant ratsAfter 4 weekly injections of 0.1 mg. estradiol benzoate.After 8 daily injections of 150 mcgm. melatonin (pineal hormone).After 8 daily injections of 3 mg. serotonin (melatonin precursor).No ultrastructural differences were evident between the control, and the pregnancy and melatonin groups. However, the estradiol injected animals exhibited a marked increase in the amount and size of rough endoplasmic reticulum within the pineal cells.

Effect of Matricaria chamomilla L. extract on fetal absorption, placenta structure and liver of diabetic pregnant rats.

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
F Namjooyan ◽  
M Panahi ◽  
F Ahmadpour ◽  
A Darvish ◽  
M Azemi ◽  
...  
Keyword(s):  
Matricaria Chamomilla ◽  
Pregnant Rats

Separation of Plasminogen Activators from Human Uterine Tissue and a Comparison with Activators from Human Urine and Porcine Tissue

1979 ◽  
Vol 41 (04) ◽  
pp. 718-733 ◽  
Author(s):  
Preben Kok
Keyword(s):  
Plasminogen Activator ◽  
Human Urine ◽  
Gel Filtration ◽  
Ammonium Thiocyanate ◽  
Plasminogen Activators ◽  
Particle Sizes ◽  
Uterine Tissue ◽  
Porcine Tissue ◽  
Major Activity ◽  
Proliferative Phase

SummaryThree types of plasminogen activator could be distinguished in extracts from human uterine tissue. The activators differed in thermostability or in mode of inhibition by EACA.All the extracts contained stable as well as labile activators. The saline extracts were uniformly inhibited by increasing concentrations of EACA. Extracts made with 2 M ammonium thiocyanate were either uniformly inhibited by EACA or showed deflections indicating contamination with an activator, which was inhibited in a biphasic manner. It was possible to distinguish between: (1) An activator, abundantly present in the tissue, which was uniformly inhibited and stable. (2) Another uniformly inhibited activator, which was labile. (3) An activator, inhibited in a biphasic manner, similar to urokinase, which was present in varying amounts in uteri with the endometrium in the proliferative phase.Gel filtration of the uterine extracts showed two major activity peaks corresponding to particle sizes of 60,000 dalton and about 10,000 dalton.Antiserum to purified plasminogen activator, prepared from porcine ovaries, inhibited the activity of the human uterine extracts, but not the activities of human urokinase or urine. Urokinase antiserum in a concentration completely inhibiting human urine or urokinase, inhibited only 10% or less of the activities of human uterine extracts.

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