Stimulation of pisatin production in Pisum sativum by actinomycin D and other compounds

1968 ◽  
Vol 126 (2) ◽  
pp. 731-733 ◽  
Author(s):  
Martin E. Schwochau ◽  
Lee A. Hadwiger
Keyword(s):  
Pisum Sativum ◽  
Actinomycin D ◽  
Stimulation Of

EFFECT OF ACTINOMYCIN D ON TSH-INDUCED STIMULATION OF THYROIDAL PROTEIN SYNTHESIS IN THE RAT

1974 ◽  
Vol 77 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Gustav Wägar
Keyword(s):  
Protein Synthesis ◽  
Actinomycin D ◽  
The Other ◽  
Leucine Incorporation ◽  
Short Term ◽  
Other Hand ◽  
Thyroid Glands ◽  
Translational Level ◽  
Stimulation Of

ABSTRACT Whether the short-term regulation of thyroidal protein synthesis by TSH occurs at the transcriptional or the translational level was tested by measuring the effect of actinomycin D (act D) on the TSH-induced stimulation of L-14C-leucine incorporation into the thyroidal proteins of rats. TSH was injected 6 h before the rats were killed. The thyroid glands were then removed and incubated in vitro in the presence of L-14C-leucine for 2 h. The pronounced stimulation of leucine incorporation in the TSH-treated animals was depressed as compared with controls but still significant even when the animals had been pre-treated with 100 μg act D 24 and 7 h before sacrifice. On the other hand, act D strongly decreased incorporation of 3H-uridine into RNA. Short-term regulation of thyroidal protein synthesis by TSH appears to be partly but not wholly dependent on neosynthesis of RNA. Hence regulation may partly occur at the translation level of protein synthesis.

scholarly journals Biosynthesis of growth hormone in the rat anterior pituitary gland. Stimulation of biosynthesis in vitro by insulin

1973 ◽  
Vol 134 (4) ◽  
pp. 1103-1113 ◽  
Author(s):  
A. Betteridge ◽  
M. Wallis
Keyword(s):  
Growth Hormone ◽  
Anterior Pituitary ◽  
Rna Synthesis ◽  
Glucose Utilization ◽  
Actinomycin D ◽  
Hormone Synthesis ◽  
Pituitary Glands ◽  
Hormone Biosynthesis ◽  
Stimulation Of

The effect of insulin on the incorporation of radioactive leucine into growth hormone was investigated by using rat anterior pituitary glands incubated in vitro. A 50% stimulation over control values was observed at insulin concentrations above 2μm (280munits/ml). The effect was specific for growth hormone biosynthesis, over the range 1–5μm-insulin (140–700munits/ml). Lower more physiological concentrations had no significant effect in this system. Above 10μm (1.4 units/ml) total protein synthesis was also increased. The stimulation of growth hormone synthesis could be partially blocked by the addition of actinomycin D, suggesting that RNA synthesis was involved. Insulin was found to stimulate the rate of glucose utilization in a similar way to growth hormone synthesis. 2-Deoxyglucose and phloridzin, which both prevented insulin from stimulating glucose utilization, also prevented the effect of insulin on growth hormone synthesis. If glucose was replaced by fructose in the medium, the effect of insulin on growth hormone synthesis was decreased. We conclude that the rate of utilization of glucose may be an important step in mediating the effect of insulin on growth hormone synthesis.

Stimulation of Leptin Release by Actinomycin D in Rat Adipocytes

1998 ◽  
Vol 55 (8) ◽  
pp. 1309-1314 ◽  
Author(s):  
John N. Fain ◽  
Suleiman W. Bahouth
Keyword(s):  
Actinomycin D ◽  
Rat Adipocytes ◽  
Stimulation Of

scholarly journals The effect of cytokinins and other plant hormones on the growth of cotyledonary axilars of flax (Linum usitatissimum), sunflower (Helianthus annuus) and pea (Pisum sativum)

2011 ◽  
Vol 50 (No. 4) ◽  
pp. 182-187 ◽  
Author(s):  
Š. Klíčová ◽  
J. Šebánek ◽  
T. Vlašic
Keyword(s):  
Pisum Sativum ◽  
Helianthus Annuus ◽  
Plant Hormones ◽  
Linum Usitatissimum ◽  
The Other ◽  
Pea Seedlings ◽  
Significant Stimulation ◽  
The Difference ◽  
Intensive Growth ◽  
Stimulation Of

Flax seedlings were decapitated above the cotyledons. After one cotyledon was removed the growth of the bud of the remaining cotyledon was stronger in 90% of the plants. However, the application of the cytokinin benzyladenine (BA) to the bud of the removed cotyledon caused a growth correlative reversal and, by contrast, in 65% of the plants the bud of the removed cotyledon grew out. On the other hand, in sunflower seedlings, which have epigeal cotyledons similar to flax, after the removal of one cotyledon the growth of the axillary of the removed cotyledon was more intensive in 59% of the plants. Not even an application of BA to the remaining cotyledon of sunflower resulted in more intensive growth of the axillary of this cotyledon. When both cotyledons were left on the seedling, BA applied to one of the cotyledons of decapitated flax plants resulted in a highly significant stimulation of growth of the axillary of this cotyledon; in sunflower; however, the effect of the BA was insignificant. After decapitation of the stem of pea seedlings where both cotyledons remained, both axillaries grew out, but after a certain period of time one of them (the dominant one) achieved a growth correlative dominance over the other (inhibited). The present study is focused on whether an application of plant hormones onto the inhibited shoot is able to cause a growth correlative reversal, i.e. to change the inhibited shoot into a dominant one. The application of 0.12% BA can cause such a reversal virtually in all plants if the original difference in the length between the inhibited and dominating axillaries is 12–24 mm. A 0.12–0.5% concentration of gibberellin causes a reversal in 13–75% of the plants, but only if the difference between the dominant and inhibited shoot is 1–12 mm. A 0.03–0.25% concentration of IAA causes a reversal in 34–57% of the plants, if the difference in the length of the axillaries is 1–4 mm.

Mechanisms of CCK regulation of monitor peptide mRNA expression in pancreatic acinar AR42J cells

1997 ◽  
Vol 272 (4) ◽  
pp. G794-G801 ◽  
Author(s):  
T. Kinouchi ◽  
S. Tsuzuki ◽  
C. Minami ◽  
Y. Hayashi ◽  
E. Sugimoto ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Actinomycin D ◽  
Mrna Level ◽  
Chain Reaction ◽  
Independent Manner ◽  
Ar42j Cells ◽  
Kinase C ◽  
A 23187 ◽  
Polymerase Chain ◽  
Stimulation Of

We explored the mechanism(s) by which cholecystokinin (CCK) stimulation of AR42J rat pancreatoma cells results in increased mRNA expression of a CCK-releasing peptide [monitor peptide (MP)]. With the use of a newly established reverse transcription-polymerase chain reaction assay system, CCK was shown to increase the level of MP mRNA by about ninefold. When protein synthesis was blocked by addition of cycloheximide, the MP mRNA level remained unchanged in the presence of CCK. Inhibition of transcription with actinomycin D resulted in a half-life for MP mRNA of approximately 17 h, and this rate remained unchanged after CCK treatment, suggesting that CCK may regulate the MP mRNA level by influencing gene transcription. A-23187, bombesin, substance P, and carbachol increased the MP mRNA level. CoCl(2) abolished actions of both CCK and A-23187 on MP mRNA expression. Dibutyryl-adenosine 3',5'-cyclic monophosphate, forskolin, secretin, and vasoactive intestinal polypeptide had no effect on MP mRNA expression. 12-O-tetradecanoylphorbol-13-acetate and phorbol 12,13-dibutyrate also failed to increase MP mRNA. It was therefore proposed that CCK stimulates MP mRNA expression of AR42J cells in a Ca2+-dependent and protein kinase C-independent manner.

EFFECTS OF AMYGDALOID LESIONS ON REGIONAL BRAIN RNA BASE RATIOS

1969 ◽  
Vol 45 (2) ◽  
pp. 207-214 ◽  
Author(s):  
B. E. ELEFTHERIOU ◽  
R. L. CHURCH ◽  
A. J. ZOLOVICK ◽  
R. L. NORMAN ◽  
M. L. PATTISON
Keyword(s):  
Actinomycin D ◽  
Normal Animal ◽  
Adenosine Monophosphate ◽  
Amygdaloid Complex ◽  
Guanosine Monophosphate ◽  
Neural Function ◽  
Regional Brain ◽  
Bilateral Lesions ◽  
Uridine Monophosphate ◽  
Stimulation Of

SUMMARY Bilateral lesions produced by electrocoagulation in the basolateral and medial amygdaloid nuclei, subtotal amygdalectomy as well as implants of actinomycin D and cholesterol were all found to have profound effects on brain ribonucleic acid (RNA) base percentages and ratios. The most consistent effect was that on the pituitary and was produced by all treatments. Generally, the hypothalamus and frontal cortex showed varying effects due to the treatments, but the cerebellum uniformly showed no responses to the various treatments. Lesions placed in the medial amygdaloid complex and subtotal amygdalectomy had the greatest effect in changing the RNA base ratio. The bases adenosine monophosphate (AMP), cytidine monophosphate (CMP), guanosine monophosphate (GMP), and uridine monophosphate (UMP) were found to be the most affected. The newly synthesized RNA was found to be rich in UMP and deficient in AMP and CMP when compared to normal animal brain tissue. The assumption is made that lesions in the amygdala bring about an activation or stimulation of a genomic nature to produce specific RNA for a particular neural function.

scholarly journals ENZYME-MEMBRANE RELATIONSHIP IN PHENOBARBITAL INDUCTION OF SYNTHESIS OF DRUG-METABOLIZING ENZYME SYSTEM AND PROLIFERATION OF ENDOPLASMIC MEMBRANES

1966 ◽  
Vol 28 (2) ◽  
pp. 181-198 ◽  
Author(s):  
Sten Orrenius ◽  
Jan L. E. Ericsson
Keyword(s):  
Actinomycin D ◽  
Enzyme System ◽  
Drug Metabolizing Enzyme ◽  
Induction Process ◽  
Autophagic Activity ◽  
Metabolizing Enzyme ◽  
Parenchymal Cells ◽  
Phenobarbital Induction ◽  
Stimulation Of

The enzyme-membrane relationship in phenobarbital induction of synthesis of drug-metabolizing enzyme system and proliferation of endoplasmic membranes has been further studied. Ultrastructural observations suggest that newly formed endoplasmic membranes in rat liver parenchymal cells arise through continuous outgrowth and budding off from pre-existing cisternae and tubules of rough-surfaced endoplasmic reticulum. The membranes induced by phenobarbital treatment persist in the cytoplasm of the hepatocyte for up to 15 days after the last of a series of 5 phenobarbital injections; the phase of regression of the induced enzymes lasts for only 5 days. Disappearance of the membranes is gradual and does not seem to be associated with increased autophagic activity in the cell. A second series of injections of phenobarbital to previously induced rats—exhibiting normal drug-hydroxylating activity but an excess of liver endoplasmic membranes—is associated with a stimulation of the rate of Pi32 incorporation into microsomal phospholipid in vivo, similar to that found during the original induction process. Administration of Actinomycin D following a single phenobarbital injection delays the regression of the enhanced drug-hydroxylating activity. Finally, the effects of Actinomycin D and puromycin on the stimulated membrane formation are discussed.

scholarly journals Digestion by pigs of non-starch polysaccharides in wheat and raw peas (Pisum sativum) fed in mixed diets

1991 ◽  
Vol 65 (2) ◽  
pp. 259-270 ◽  
Author(s):  
J. S. Goodlad ◽  
J. C. Mathers
Keyword(s):  
Fatty Acids ◽  
Linear Regression ◽  
Pisum Sativum ◽  
Multiple Linear Regression ◽  
Volatile Fatty Acids ◽  
Bacterial Biomass ◽  
Diaminopimelic Acid ◽  
Inclusion Rate ◽  
Linear Regression Technique ◽  
Stimulation Of

The digestion by pigs of non-starch polysaccharides (NSP) in wheat and raw peas (Pisum sativum) fed in mixed diets was measured. In the four experimental diets, wheat was included at a constant 500 g/kg whilst peas contributed 0–300 g/kg and these were the only dietary sources of NSP. Separate estimates of digestibility for wheat and peas were obtained by using a multiple linear regression technique which also tested the possibility that the presence of peas might influence the digestibility of wheat NSP. There was little evidence of the latter and it was found that the digestibility of peas NSP (0.84) was considerably greater than that of wheat (0.65). The non-cellulosic polysaccharides (NCP) had twofold greater digestibilities than had cellulose for both foods with essentially all the peas NCP being digested. Faecal α-diaminopimelic acid concentration increased with feeding of peas, suggesting stimulation of bacterial biomass production in the large intestine using the readily fermented peas NSP. All three major volatile fatty acids produced by large intestinal fermentation were detected in jugular blood and increased significantly with increasing peas inclusion rate in the diet.

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