Human salivary proteins and artificial calculus formation in vitro

1965 ◽  
Vol 10 (2) ◽  
pp. 227-235 ◽  
Author(s):  
J.D. Mlddleton
Keyword(s):  
Salivary Proteins ◽  
Calculus Formation

A predictive model for astringency based on in vitro interactions between salivary proteins and (−)-Epigallocatechin gallate

2021 ◽  
Vol 340 ◽  
pp. 127845 ◽  
Author(s):  
Qing-Qing Ye ◽  
Gen-Sheng Chen ◽  
Weichun Pan ◽  
Qing-Qing Cao ◽  
Liang Zeng ◽  
...  
Keyword(s):  
Predictive Model ◽  
Epigallocatechin Gallate ◽  
Salivary Proteins ◽  
In Vitro Interactions

In Vitro Studies of the Calculus-Inhibiting Properties of Tooth Surface Polishing Agents and Chelating Agents

1971 ◽  
Vol 50 (4) ◽  
pp. 966-975 ◽  
Author(s):  
George P. Barnes ◽  
George K. Stookey ◽  
Joseph C. Muhler
Keyword(s):  
Chelating Agents ◽  
Magnesium Silicate ◽  
In Vitro Studies ◽  
Tooth Surface ◽  
Surface Polishing ◽  
Zirconium Silicate ◽  
Human Dentin ◽  
Calculus Formation ◽  
Bovine Enamel

A series of in vitro studies was conducted to determine the effects of flour of pumice, magnesium silicate-zirconium silicate, and zirconium silicate on the deposition of calculuslike material on bovine enamel sections or human dentin-cementum sections. Flour of pumice used as a "prophylaxis type" agent did not have any effect on the deposition of calculuslike material. Zirconium silicate used as a prophylactic agent on bovine enamel reduced in vitro calculus formation by 32.0 to 38.0%. Daily rinses with a 5.0% aqueous solution of an amidopolyphosphate reduced calculus formation significantly on bovine enamel by 57.0 to 68.2% when compared with nontreated enamel sections.

In vitro hydroxyapatite adsorbed salivary proteins

2004 ◽  
Vol 320 (2) ◽  
pp. 342-346 ◽  
Author(s):  
Rui Vitorino ◽  
Maria João C Lobo ◽  
José Duarte ◽  
António J Ferrer-Correia ◽  
Kenneth B Tomer ◽  
...  
Keyword(s):  
Salivary Proteins

Salivary Proteins Interact with Dietary Constituents to Modulate Tooth Staining

2005 ◽  
Vol 84 (1) ◽  
pp. 73-78 ◽  
Author(s):  
G.B. Proctor ◽  
R. Pramanik ◽  
G.H. Carpenter ◽  
G.D. Rees
Keyword(s):  
Red Wine ◽  
Black Tea ◽  
Salivary Protein ◽  
Salivary Proteins ◽  
Parotid Saliva ◽  
Grape Skin ◽  
Dietary Components ◽  
Black Tea Polyphenols ◽  
Proline Rich Protein

Dietary components rich in polyphenols—for example, tea and red wine—are thought to cause tooth staining. In the present study, hydroxyapatite was used as a model of enamel for study of the influence of salivary proteins on the binding of different polyphenols to hydroxyapatite in vitro. Neither salivary protein pellicles nor salivary proteins in solution significantly altered the binding of the small polyphenol epigallocatechin to hydroxyapatite. However, hydroxyapatite binding of anthocyanin, a small grape-skin-derived polyphenol, or the larger polyphenols of black tea was increased by the presence of salivary proteins, either as a pellicle or in solution. Proline-rich proteins were enriched from parotid saliva and found to increase binding of anthocyanin and black tea polyphenols to hydroxyapatite, while enriched histatins did not increase binding. It is concluded that some salivary proteins, including proline-rich protein, can mediate increased staining of enamel by red-wine- and black-tea-derived polyphenols.

In Vitro Calculus Formation

1960 ◽  
Vol 39 (5) ◽  
pp. 1041-1048 ◽  
Author(s):  
Frithjof Von der Fehr ◽  
Finn Brudevold
Keyword(s):  
Calculus Formation

Saliva and Dental Pellicle-A Review

2000 ◽  
Vol 14 (1) ◽  
pp. 22-28 ◽  
Author(s):  
U. Lendenmann ◽  
J. Grogan ◽  
F.G. Oppenheim
Keyword(s):  
Biochemical Characterization ◽  
Tooth Enamel ◽  
Selective Adsorption ◽  
Salivary Proteins ◽  
Single Donor ◽  
Composition And Structure ◽  
Acquired Enamel Pellicle

The acquired enamel pellicle is an organic film covering the surfaces of teeth. When this film was first discovered, it was thought to be of embryologic origin. Only in the middle of this century did it become clear that it was acquired after tooth eruption. Initially, the small amounts of material that could be obtained have virtually limited the investigation of pellicle proteins to amino acid analysis. Nevertheless, this technique revealed that the pellicle is mainly proteinaceous and is formed by selective adsorption of salivary proteins on tooth enamel. Later, immunologic techniques allowed for the identification of many salivary and fewer non-salivary proteins as constituents of pellicle. However, to this date, isolation and direct biochemical characterization of in vivo pellicle protein have not been possible, because only a few micrograms can be obtained from a single donor. Therefore, the composition and structure of the acquired enamel pellicle are still essentially unknown. Information on the functions of pellicle has been obtained mainly from in vitro experiments carried out with saliva-coated hydroxyapatite and enamel discs. It was found that pellicle protects enamel by reducing demineralization upon acid challenge. Improved pellicle harvesting procedures and analysis by state-of-the-art proteomics with mass spectroscopy approaches promise to make major inroads into the characterization of enamel pellicle.

scholarly journals In Vitro Salivary Protein Adsorption Profile on Titanium and Ceramic Surfaces and the Corresponding Putative Immunological Implications

2020 ◽  
Vol 21 (9) ◽  
pp. 3083 ◽  
Author(s):  
Chen-Xuan Wei ◽  
Michael Francis Burrow ◽  
Michael George Botelho ◽  
Henry Lam ◽  
Wai Keung Leung
Keyword(s):  
Immune Responses ◽  
Interaction Network ◽  
Salivary Protein ◽  
Microbial Interactions ◽  
Salivary Proteins ◽  
Material Surface ◽  
Go Annotation ◽  
Implant Abutment ◽  
Adsorbed Proteins

Immune responses triggered by implant abutment surfaces contributed by surface-adsorbed proteins are critical in clinical implant integration. How material surface-adsorbed proteins relate to host immune responses remain unclear. This study aimed to profile and address the immunological roles of surface-adsorbed salivary proteins on conventional implant abutment materials. Standardized polished bocks (5 × 5 × 1 mm3) were prepared from titanium and feldspathic ceramic. Salivary acquired pellicle formed in vitro was examined by liquid chromatography-tandem mass spectrometry and gene ontology (GO) analysis to identify and characterize the adsorbed proteins. Out of 759 proteins identified from pooled saliva samples, 396 were found to be attached to the two materials tested—369 on titanium and 298 on ceramic, with 281 common to both. GO annotation of immune processes was undertaken to form a protein–protein interaction network, and 14 hub proteins (≥6 interaction partners) (coding genes: B2M, C3, CLU, DEFA1, HSP90AA1, HSP90AB1, LTF, PIGR, PSMA2, RAC1, RAP1A, S100A8, S100A9, and SLP1) were identified as the key proteins connecting multiple (6–9) immune processes. The results offered putative immunological prospects of implant abutment material surface-adsorbed salivary proteins, which could potentially underpin the dynamic nature of implant–mucosal/implant–microbial interactions.

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