Growth hormone effects on histidine incorporation into protein by adipose tissue from hypophysectomized rats

1968 ◽  
Vol 169 (2) ◽  
pp. 556-558 ◽  
Author(s):  
Norbert I. Swislocki
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Hormone Effects ◽  
Hypophysectomized Rats

DOSE REGIMENS OF HUMAN GROWTH HORMONE: EFFECTS OF CONTINUOUS INFUSION AND OF A GELATIN VEHICLE ON GROWTH IN RATS AND RATE OF ABSORPTION IN RABBITS

1980 ◽  
Vol 87 (2) ◽  
pp. 303-312 ◽  
Author(s):  
P. MARY COTES ◽  
W. A. BARTLETT ◽  
ROSE E. GAINES DAS ◽  
P. FLECKNELL ◽  
R. TERMEER
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Plasma Levels ◽  
Time Course ◽  
Human Growth ◽  
Daily Injection ◽  
Glycine Buffer ◽  
Hormone Effects ◽  
Rate Of Absorption ◽  
Hypophysectomized Rats

Different methods for administration of human growth hormone (hGH) have been examined with a view to efficient use of the limited amounts of hGH at present available for clinical use. We found that in hypophysectomized rats (1) hGH administered by continuous subcutaneous infusion induced a greater increase in body weight (referred to throughout as growth) than hGH administered by intermittent (daily) injection and (2) intermittent injections of hGH dissolved in 16% gelatin induced more growth than hGH dissolved in a glycine buffer. It was further found that (1) hGH dissolved in 16% gelatin compared with hGH dissolved in a glycine buffer induced lower maximal levels of immunoreactive plasma hGH and between 7 and 9 h after treatment higher plasma levels when injected subcutaneously in rabbits, (2) 125I-labelled hGH added as a tracer to hGH in gelatin was removed more slowly from subcutaneous injection sites in rabbits than 125I-labelled hGH given with hGH in glycine buffer and (3) changes in the ratio of hGH to gelatin had little effect on the time-course of plasma levels of hGH in the rabbit. Addition of the protease inhibitors aprotinin or 6-aminohexanoic acid, to injection of hGH in gelatin or glycine did not induce any consistent increase in plasma levels of hGH.

scholarly journals Influence of hormones on the nicotinamide nucleotide coenzymes of adipose tissue

1967 ◽  
Vol 105 (3) ◽  
pp. 1013-1018 ◽  
Author(s):  
Patricia McLean ◽  
A. L. Greenbaum ◽  
J. Brown ◽  
K. R. Greenslade
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Lipid Synthesis ◽  
Total Content ◽  
Diabetic Rats ◽  
Tissue Protein ◽  
Significant Difference ◽  
Hypophysectomized Rats ◽  
Reduced Forms ◽  
Fat Pads

The concentrations of the oxidized and reduced forms of the nicotinamide nucleotides were measured in the epididymal fat pads of normal, alloxan-diabetic and hypophysectomized rats. In both alloxan-diabetic rats and hypophysectomized rats the weight of the adipose tissue fell, as did the total content of NADH and NADPH; in addition, NAD+ was decreased in the alloxan-diabetic group. Of these changes the most marked was in NADPH and this was the only significant difference when the results were expressed as nicotinamide nucleotides/mg. of tissue protein. The concentration of NADPH in the hypophysectomized rats was not altered by treatment with growth hormone but was restored to normal by treatment with thyroxine. These results are discussed in relation to the known effect of these hormonal conditions on lipid synthesis in adipose tissue.

Effect of Insulin Treatment of Hypophysectomized Rats on Adipose Tissue Responsiveness to Insulin and Growth Hormone*

Endocrinology ◽  
1985 ◽  
Vol 116 (3) ◽  
pp. 945-951 ◽  
Author(s):  
INGRID GAUSE ◽  
OLLE ISAKSSON ◽  
ANDERS LINDAHL ◽  
STAFFAN EDÉN
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Insulin Treatment ◽  
Hypophysectomized Rats

Glucose transport in adipocytes and its control by growth hormone in vivo

1982 ◽  
Vol 242 (6) ◽  
pp. E368-E372 ◽  
Author(s):  
E. Schoenle ◽  
J. Zapf ◽  
E. R. Froesch
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Adipose Tissue ◽  
Glucose Transport ◽  
Human Growth ◽  
Limiting Factor ◽  
Glucose Transport System ◽  
Hypophysectomized Rats ◽  
Tibial Epiphyseal

Previous results showed maximally enhanced basal glucose transport in adipocytes of hypophysectomized rats and restoration to normal after human growth hormone (hGH) administration. The data suggested a hGH-dependent "limiting factor" for glucose transport in the adipocyte membrane, which is acutely inhibited by insulin resulting in enhanced glucose transport. In this study the effect of hGH was investigated with respect to dose and time dependence. hGH was administered by continuous infusion from subcutaneously implanted Alzet minipumps. A significant decrease of basal glucose transport was obtained at the lowest hGH dose of 50 mU/day for 6 days. This effect of hGH was strictly correlated to the effects on growth (tibial epiphyseal width, DNA synthesis, body weight, serum level of insulin-like growth factor). The effect of hGH on basal glucose transport was already observed after 12 h of infusion, and it increased to a maximum after 3 days. The data support the concept that GH regulates the glucose transport system in adipose tissue in vivo.

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