The release of endonuclease I from Escherichia coli by a new cold shock procedure

When cells of Escherichia coli were suspended in dilute artificial seawater and cooled to various subzero temperatures, a maximum lethal effect occurred around −40 °C. In addition, rapid cooling to −26 °C of bacteria, suspended in concentrated, non-freezing seawater caused a pronounced decrease in viability ("cold shock"). The loss in viability was accompanied by a proportional release from the cells of ultraviolet-absorbing material and by an increase in the ribose and organic phosphorus contents of the suspending liquid. It seems possible that the released material, at least partly, consisted of nucleotides or nucleic acids.

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Major cold shock protein of Escherichia coli.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.87.1.283 ◽

1990 ◽

Vol 87 (1) ◽

pp. 283-287 ◽

Cited By ~ 320

Author(s):

J. Goldstein ◽

N. S. Pollitt ◽

M. Inouye

Keyword(s):

Escherichia Coli ◽

Cold Shock ◽

Cold Shock Protein

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Characterization of cspB, a cold-shock-inducible gene from Lactococcus lactis, and evidence for a family of genes homologous to the Escherichia coli cspA major cold shock gene.

Journal of Bacteriology ◽

10.1128/jb.179.17.5589-5593.1997 ◽

1997 ◽

Vol 179 (17) ◽

pp. 5589-5593 ◽

Cited By ~ 20

Author(s):

M P Chapot-Chartier ◽

C Schouler ◽

A S Lepeuple ◽

J C Gripon ◽

M C Chopin

Keyword(s):

Escherichia Coli ◽

Lactococcus Lactis ◽

Cold Shock ◽

Shock Gene ◽

Inducible Gene

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Solution NMR Structure of Ribosome-binding Factor A (RbfA), A Cold-shock Adaptation Protein from Escherichia coli

Journal of Molecular Biology ◽

10.1016/s0022-2836(03)00061-5 ◽

2003 ◽

Vol 327 (2) ◽

pp. 521-536 ◽

Cited By ~ 49

Author(s):

Yuanpeng Janet Huang ◽

G.V.T. Swapna ◽

P.K. Rajan ◽

Haiping Ke ◽

Bing Xia ◽

...

Keyword(s):

Escherichia Coli ◽

Cold Shock ◽

Nmr Structure ◽

Solution Nmr ◽

Ribosome Binding ◽

Binding Factor

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Cold Shock Induces Chromosomal qnr in Vibrio Species and Plasmid-Mediated qnrS1 in Escherichia coli

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01472-19 ◽

2019 ◽

Vol 63 (12) ◽

Author(s):

Hee-Chang Jang ◽

Yin Wang ◽

Chunhui Chen ◽

Laura Vinué ◽

George A. Jacoby ◽

...

Keyword(s):

Escherichia Coli ◽

Bacterial Community ◽

Vibrio Parahaemolyticus ◽

Cold Shock ◽

Vibrio Vulnificus ◽

Wild Type ◽

Induced Expression ◽

Content Type ◽

E Coli ◽

Dna Damaging Agents

ABSTRACT qnr genes are found in aquatic bacteria and were present in the bacterial community before the introduction of synthetic quinolones. Their natural functions are unknown. We evaluated expression of chromosomal qnr in Vibrio species in response to environmental stresses and DNA-damaging agents. Subinhibitory concentrations of quinolones, but not other DNA-damaging agents, increased expression of chromosomal qnr by more than five times in Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio mytili. Cold shock also induced expression of qnr in V. parahaemolyticus, V. vulnificus, and V. mytili, as well as expression of qnrS1 in Escherichia coli. qnrS1 induction by cold shock was not altered in ΔihfA or ΔihfB mutants or in a strain overexpressing dnaA, all of which otherwise directly modulate qnrS1 induction by ciprofloxacin. In contrast, the level of qnrS1 induction by cold shock was reduced in a ΔcspA mutant in the cold shock regulon compared to the wild type. In conclusion, cold shock and quinolones induce expression of chromosomal qnr in Vibrio species and of the related qnrS1 gene in E. coli.

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