Immunocytochemical detection in eel corpuscles of stannius of a mammalian parathyroid-like hormone

1984 ◽  
Vol 53 (1) ◽  
pp. 28-36 ◽  
Author(s):  
Evelyne Lopez ◽  
Eva-Maria Tisserand-Jochem ◽  
André Eyquem ◽  
Christian Milet ◽  
Carmel Hillyard ◽  
...  
Keyword(s):  
Corpuscles Of Stannius ◽  
Immunocytochemical Detection

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

NeuN As a Neuronal Nuclear Antigen and Neuron Differentiation Marker

Acta Naturae ◽  
2015 ◽  
Vol 7 (2) ◽  
pp. 42-47 ◽  
Author(s):  
V. V. Gusel’nikova ◽  
D. E. Korzhevskiy
Keyword(s):  
Experimental Data ◽  
Intracellular Localization ◽  
Nuclear Antigen ◽  
Structure And Properties ◽  
Immunocytochemical Detection ◽  
Perinuclear Cytoplasm ◽  
Diagnosis Of Cancer ◽  
Morphological Diagnosis ◽  
The Central Nervous System ◽  
Differentiation Marker

The NeuN protein is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. Monoclonal antibodies to the NeuN protein have been actively used in the immunohistochemical research of neuronal differentiation to assess the functional state of neurons in norm and pathology for more than 20 years. Recently, NeuN antibodies have begun to be applied in the differential morphological diagnosis of cancer. However, the structure of the protein, which can be revealed by antibodies to NeuN, remained unknown until recently, and the functions of the protein are still not fully clear. In the present mini-review, data on NeuN accumulated so far are summarized and analyzed. Data on the structure and properties of the protein, its isoforms, intracellular localization, and hypothesized functions are reported. The application field of immunocytochemical detection of NeuN in scientific and clinical studies, as well as the difficulties in the interpretation of the obtained experimental data and their possible causes, is described in details.

Immunocytochemical detection of Fcγ receptors in human atherosclerotic lesions

2001 ◽  
Vol 77 (3) ◽  
pp. 169-174 ◽  
Author(s):  
Nora R Ratcliffe ◽  
Susan M Kennedy ◽  
Peter M ◽  
Morganelli
Keyword(s):  
Fcγ Receptors ◽  
Atherosclerotic Lesions ◽  
Immunocytochemical Detection

Presence of brain spectrin in dendrites of mammalian brain: Technical factors involved in immunocytochemical detection

Synapse ◽  
1988 ◽  
Vol 2 (3) ◽  
pp. 329-333 ◽  
Author(s):  
Gwen O. Ivy ◽  
Peter Seubert ◽  
Michel Baudry ◽  
Gary Lynch
Keyword(s):  
Mammalian Brain ◽  
Immunocytochemical Detection ◽  
Technical Factors ◽  
Brain Spectrin
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