Low-frequency electromagnetic fields induce premature terminal differentiation of in-vitro cultured human skin and lung fibroblasts

1992 ◽  
Vol 342 (3) ◽  
pp. 269-280 ◽  
Author(s):  
H.Peter Rodemann ◽  
Petra Wecke ◽  
Monika Jaeschke
Keyword(s):  
Human Skin ◽  
Electromagnetic Fields ◽  
Low Frequency ◽  
Terminal Differentiation ◽  
Lung Fibroblasts

scholarly journals Human Gingival Fibroblasts Exposed to Extremely Low-Frequency Electromagnetic Fields: In Vitro Model of Wound-Healing Improvement

2019 ◽  
Vol 20 (9) ◽  
pp. 2108 ◽  
Author(s):  
Erica Costantini ◽  
Bruna Sinjari ◽  
Chiara D’Angelo ◽  
Giovanna Murmura ◽  
Marcella Reale ◽  
...  
Keyword(s):  
Wound Healing ◽  
Electromagnetic Field ◽  
Electromagnetic Fields ◽  
Wound Repair ◽  
Healing Process ◽  
Low Frequency ◽  
Gingival Fibroblasts ◽  
Field Exposure ◽  
Extremely Low Frequency

Several clinical studies have suggested the impact of sinusoidal and pulsed electromagnetic fields in quickening wound repair processes and tissue regeneration. The clinical use of extremely low-frequency electromagnetic fields could represent a novel frontier in tissue repair and oral health, with an interesting clinical perspective. The present study aimed to evaluate the effect of an extremely low-frequency sinusoidal electromagnetic field (SEMF) and an extremely low-frequency pulsed electromagnetic field (PEMF) with flux densities of 1 mT on a model of oral healing process using gingival fibroblasts. An in vitro mechanical injury was produced to evaluate wound healing, migration, viability, metabolism, and the expression of selected cytokines and protease genes in fibroblasts exposed to or not exposed to the SEMF and the PEMF. Interleukin 6 (IL-6), transforming growth factor beta 1 (TGF-β), metalloproteinase 2 (MMP-2), monocyte chemoattractant protein 1 (MCP-1), inducible nitric oxide synthase (iNOS), and heme oxygenase 1 (HO-1) are involved in wound healing and tissue regeneration, favoring fibroblast proliferation, chemotaxis, and activation. Our results show that the exposure to each type of electromagnetic field increases the early expression of IL-6, TGF-β, and iNOS, driving a shift from an inflammatory to a proliferative phase of wound repair. Additionally, a later induction of MMP-2, MCP-1, and HO-1 was observed after electromagnetic field exposure, which quickened the wound-healing process. Moreover, electromagnetic field exposure influenced the proliferation, migration, and metabolism of human gingival fibroblasts compared to sham-exposed cells. This study suggests that exposure to SEMF and PEMF could be an interesting new non-invasive treatment option for wound healing. However, additional studies are needed to elucidate the best exposure conditions to provide the desired in vivo treatment efficacy.

scholarly journals CT2A Cell Viability Modulated by Electromagnetic Fields at Extremely Low Frequency under No Thermal Effects

2019 ◽  
Vol 21 (1) ◽  
pp. 152 ◽  
Author(s):  
Olga García-Minguillán ◽  
Raquel Prous ◽  
Maria del Carmen Ramirez-Castillejo ◽  
Ceferino Maestú
Keyword(s):  
Cell Viability ◽  
Frequency Dependence ◽  
Electromagnetic Fields ◽  
Thermal Effects ◽  
Low Frequency ◽  
Human Beings ◽  
Cellular Mechanisms ◽  
Low Frequencies ◽  
Hsp90 Expression

The effects produced by electromagnetic fields (EMFs) on human beings at extremely low frequencies (ELFs) have being investigated in the past years, across in vitro studies, using different cell lines. Nevertheless, the effects produced on cells are not clarified, and the cellular mechanisms and cell-signaling processes involved are still unknown. This situation has resulted in a division among the scientific community about the adequacy of the recommended level of exposure. In this sense, we consider that it is necessary to develop long-term exposure studies and check if the recommended levels of EMFs are under thermal effects. Hence, we exposed CT2A cells to different EMFs at different ELFs at short and long times. Our results showed frequency dependence in CT2A exposed during 24 h to a small EMF of 30 μT equal to those originated by the Earth and frequency dependence after the exposure during seven days to an EMF of 100 µT at different ELFs. Particularly, our results showed a remarkable cell viability decrease of CT2A cells exposed to EMFs of 30 Hz. Nevertheless, after analyzing the thermal effects in terms of HSP90 expression, we did not find thermal damages related to the differences in cell viability, so other crucial cellular mechanism should be involved.

scholarly journals Induction of Cell Activation Processes by Low Frequency Electromagnetic Fields

2004 ◽  
Vol 4 ◽  
pp. 4-22 ◽  
Author(s):  
Myrtill Simkó
Keyword(s):  
Electromagnetic Fields ◽  
Cellular Response ◽  
Cell Activation ◽  
Childhood Leukemia ◽  
Mechanistic Model ◽  
Cell Cycle Control ◽  
Low Frequency ◽  
Cellular Systems ◽  
Increased Risk

Electromagnetic fields (EMF) such as those from electric power transmission and distribution lines (50/60 Hz) have been associated with increased risk of childhood leukemia, cancer of the nervous system, and lymphomas. Severalin vitrostudies on EMF effects were performed to clarify the existing controversies, define the risks, and determine the possible mechanisms of adverse effects. In some of these reports, the effects were related to other mechanisms of carcinogenesis. Modification in cell proliferation was observed after EMF exposure and a few reports on cytotoxic effects have also been published. This limited review gives an overview of the current results of scientific research regardingin vitrostudies on the effects of power line frequency EMF, but also cell biological mechanisms and their potential involvement in genotoxicity and cytotoxicity are discussed. Cell cycle control and signal transduction processes are included to elucidate the biochemical background of possible interactions. Exposure to EMF has been also linked to the incidence of leukemia and other tumors in some epidemiological studies and is considered as “possibly carcinogenic to humans”, but there is no well-established biological mechanism that explains such a relation. Furthermore, EMF is also shown as a stimulus for immune relevant cells (e.g., macrophages) to release free radicals. It is known that chronic activation of macrophages is associated with the onset of phagocytosis and leads to increased formation of reactive oxygen species, which themselves may cause DNA damage and are suggested to lead to carcinogenesis. To demonstrate a possible interaction between EMF and cellular systems, we present a mechanistic model describing cell activation as a major importance for cellular response.

Effect of extremely low frequency electromagnetic fields on thromboxane B2-release by ionophore-stimulated neutrophils in vitro

1984 ◽  
Vol 27 ◽  
pp. 96 ◽  
Author(s):  
P. Conti ◽  
G.E. Gigante ◽  
M.G. Cifone ◽  
E. Alesse ◽  
G. Ianni ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Low Frequency ◽  
Thromboxane B2 ◽  
Extremely Low Frequency

Extremely low-frequency electromagnetic fields promote in vitro neurogenesis via upregulation of Cav1-channel activity

2008 ◽  
Vol 215 (1) ◽  
pp. 129-139 ◽  
Author(s):  
Roberto Piacentini ◽  
Cristian Ripoli ◽  
Daniele Mezzogori ◽  
Gian Battista Azzena ◽  
Claudio Grassi
Keyword(s):  
Electromagnetic Fields ◽  
Low Frequency ◽  
Channel Activity ◽  
Extremely Low Frequency

Therapeutic Application of Musically Modulated Electromagnetic Fields in the Treatment of Musculoskeletal Disorders

2012 ◽  
Vol 10 (3) ◽  
pp. 257-267
Author(s):  
C. Corallo ◽  
M. Rigato ◽  
E. Battisti ◽  
A. Albanese ◽  
S. Gonnelli ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Musculoskeletal Disorders ◽  
Electromagnetic Fields ◽  
Experimental Studies ◽  
Low Frequency ◽  
Experimental Point ◽  
Therapeutic Application ◽  
Extremely Low Frequency ◽  
Positive Effects

Different studies have demonstrated the efficacy of extremely low frequency electromagnetic fields (ELF EMFs) in the treatment of pain. In particular, the positive effects of ELF EMFs seems to depend on their respective codes, such as frequency, intensity and waveform, even if the exact mechanism of interaction is still debated. The most commonly used for extremely low frequency magnetotherapy is a 100Hz sinusoidal field (ELF) with a mean of induction of few Gauss. This article reviews the therapeutic application of a musically modulated electromagnetic field (TAMMEF), a new-generation of electromagnetic field used for extremely low frequency magnetotherapy characterized by variable frequencies, intensities and waveforms. Both clinical and experimental studies, performed by authors of the present review, have demonstrated the efficacy of ELF and the new TAMMEF systems in several musculoskeletal disorders such as osteoarthritis, rheumatoid arthritis, carpal tunnel syndrome, shoulder periarthritis and cervical spondylosis. Moreover, it has been demonstrated that ELF and TAMMEF systems are not only effective, but also safe, from clinical and experimental point of view. In fact, clinical trials did not reported any undesired side effect, while in vitro studies showed that ELF EMFs did not induce uncontrolled cell proliferation, did not affect cell viability and did not induce apoptosis. With their efficacy and safety, ELF and even more the new TAMMEF systems represent a valid complementary or alternative treatment to standard pharmacological therapies in reducing both pain and inflammation of patients affected by musculoskeletal disorders.

Sign in / Sign up

Export Citation Format

Share Document