Burn shock in untreated and saline-resuscitated guinea pigs development of a model

1976 ◽  
Vol 21 (4) ◽  
pp. 269-276 ◽  
Author(s):  
Robert R. Wolfe ◽  
Harvey I. Miller
Keyword(s):  
Guinea Pigs ◽  
Burn Shock

Cardiovascular and metabolic responses during burn shock in the guinea pig

1976 ◽  
Vol 231 (3) ◽  
pp. 892-897 ◽  
Author(s):  
RR Wolfe ◽  
HI Miller
Keyword(s):  
Cardiac Output ◽  
Oxygen Consumption ◽  
Guinea Pig ◽  
Core Temperature ◽  
Plasma Glucose ◽  
Guinea Pigs ◽  
Tissue Perfusion ◽  
Metabolic Responses ◽  
Burn Shock ◽  
Lactate Levels

The hemodynamic and metabolic responses of fatally burned, nonfatally burned, and unburned control guinea pigs were compared. The burns were induced in temporarily anesthetized animals by immersion to either the xyphoid process (70% fatal) or the midabdomen (100%survival) in boiling water for 3 s. Although cardiac output was reduced in all animals postburn, the survivors (MAG) has higher cardiac outputs at lower arterial pressures than the nonsurvivors (XPN). The postburn lactate levels in the XPN were higher than in the MAG, and the postburn values for pH, oxygen consumption, and core temperature were lower in the XPN. In each group, hyperglycemia was evident for 8 h postburn and terminal plasma glucose concentrations were usually elevated or similar to the prevalue. It was concluded that fatal and nonfatal burn shock were distinguished primarily by differences in tissue perfusion.

scholarly journals Microscopic and submicroscopic structure of the heart atria and auricles in condition of the experimental thermal trauma

2020 ◽  
Vol 10 (2) ◽  
pp. 5237-5242
Keyword(s):  
Guinea Pigs ◽  
Muscle Fibers ◽  
Relative Volume ◽  
The Body ◽  
Burn Shock ◽  
Structural Components ◽  
Ether Anesthesia ◽  
Submicroscopic Structure ◽  
Thermal Trauma ◽  
Heart Atria

Burns is the damage of the skin or mucous membranes (often with underlying tissues), because of high-temperature influence (thermal burns), chemical, electrical, radiation, combined, thermochemical and electrothermal factors influence. According to the investigations, burns rank third among other injuries, and in some countries, second, second only to transport injuries. Although the level of survival after burns is increasing, the problem of their treatment remains relevant in modern medicine and pharmacy. The aim was to study the peculiarities and the patterns of morphofunctional changes in the structural components of the heart atria and auricles in case of the experimental thermal trauma. The study involves 30 adult guinea pigs with a bodyweight of 630-670 g. The burn was applied under general ether anesthesia with water vapor at a temperature of 96-97 0C on the surface of the skin of the animal's back for 60 seconds. The size of the lesion area was 18-20% of the body surface. The depth of the lesion corresponded to the degree ІІІА -IIIB. The experimental animals were divided into two groups: the first - intact guinea pigs (6 heads); second - animals with thermal trauma (24 heads). Were used a number of methods of investigation, including macrometric and massometric - to establish the structural restructuring of the heart and its parts, microscopic and electron microscopic to detect changes in the structural components of the atrium and auricle of the heart; morphometric - to obtain quantitative parameters of the morphological components of the heart; mathematical and statistical - to ensure the analysis of the reliability of the research results. In intact guinea pigs, the morphological organization of the heart auricles differs from the atria by the direction of the muscle fibers, the ratio of morphometric parameters of their structural components and the content of muscle endocrine cells. Submicroscopically, endocrine myocytes of the auricles of the heart contain more hormonal granules than atria. In the ears of the heart, the relative volume of connective tissue is 1,34 times greater, and the relative volume of muscle fibers is 1,03 times smaller than in the atria. Submicroscopically, endocrine muscle cells of the heart auricle contain more hormonal granules than atria. It has been established that thermal trauma causes significant morphofunctional changes in the atria and auricles of the heart at different levels of their structural organization. The degree of damage depends on the duration of the experiment and develops against the background of increased plasma toxicity. In the stages of burn shock and early toxemia, adaptive-compensatory processes develop in the structures of the heart, and irreversible destructive changes occur in the stages of late toxemia and septicotoxemia.

Investigation of the relaxant effects of propofol on ovalbumin-induced asthma in guinea pigs

2006 ◽  
pp. 1
Author(s):  
I. Bagcivan ◽  
O. Cevit ◽  
M. K. Yildirim ◽  
S. Gursoy ◽  
S. Yildirim ◽  
...  
Keyword(s):  
Guinea Pigs

Hepatocyte Alterations in Guinea Pigs FED Polychlorinated Biphenyls (PCBs), Dibenzodioxins (PCDD), AND DIBENZOFURANS (PCDF)

1982 ◽  
Vol 40 ◽  
pp. 56-57
Author(s):  
J. N. Turner ◽  
D. N. Collins
Keyword(s):  
Guinea Pigs ◽  
Room Temperature ◽  
Dose Group ◽  
Methyl Cellulose ◽  
Uranyl Acetate ◽  
Target Organ ◽  
Office Building ◽  
Lead Citrate ◽  
Control Groups ◽  
Cooling Fluid

A fire involving an electric service transformer and its cooling fluid, a mixture of PCBs and chlorinated benzenes, contaminated an office building with a fine soot. Chemical analysis showed PCDDs and PCDFs including the highly toxic tetra isomers. Guinea pigs were chosen as an experimental animal to test the soot's toxicity because of their sensitivity to these compounds, and the liver was examined because it is a target organ. The soot was suspended in 0.75% methyl cellulose and administered in a single dose by gavage at levels of 1,10,100, and 500mgm soot/kgm body weight. Each dose group was composed of 6 males and 6 females. Control groups included 12 (6 male, 6 female) animals fed activated carbon in methyl cellulose, 6 males fed methyl cellulose, and 16 males and 10 females untreated. The guinea pigs were sacrificed at 42 days by suffocation in CO2. Liver samples were immediately immersed and minced in 2% gluteraldehyde in cacadylate buffer at pH 7.4 and 4°C. After overnight fixation, samples were postfixed in 1% OsO4 in cacodylate for 1 hr at room temperature, embedded in epon, sectioned and stained with uranyl acetate and lead citrate.

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

1983 ◽  
Vol 41 ◽  
pp. 726-727
Author(s):  
Corazon D. Bucana
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Electron Density ◽  
Peritoneal Cavity ◽  
Ultrastructural Study ◽  
Guinea Pigs ◽  
Random Distribution ◽  
Glycogen Content ◽  
Polymorphonuclear Neutrophils ◽  
Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Annulate lamellae in the Sertoli cells of guinea pig testis after unilateral torsion of the spermatic cord

1984 ◽  
Vol 42 ◽  
pp. 196-197
Author(s):  
J. Chakraborty ◽  
A. P. Sinha Hikim ◽  
J. S. Jhunjhunwala
Keyword(s):  
Sertoli Cells ◽  
Guinea Pigs ◽  
Spermatic Cord ◽  
Present Report ◽  
Cell Types ◽  
Annulate Lamellae ◽  
Spermatogenic Cells ◽  
Electron Microscopic ◽  
Structural Details ◽  
First Time

Although the presence of annulate lamellae was noted in many cell types, including the rat spermatogenic cells, this structure was never reported in the Sertoli cells of any rodent species. The present report is based on a part of our project on the effect of torsion of the spermatic cord to the contralateral testis. This paper describes for the first time, the fine structural details of the annulate lamellae in the Sertoli cells of damaged testis from guinea pigs.One side of the spermatic cord of each of six Hartly strain adult guinea pigs was surgically twisted (540°) under pentobarbital anesthesia (1). Four months after induction of torsion, animals were sacrificed, testes were excised and processed for the light and electron microscopic investigations. In the damaged testis, the majority of seminiferous tubule contained a layer of Sertoli cells with occasional spermatogonia (Fig. 1). Nuclei of these Sertoli cells were highly pleomorphic and contained small chromatinic clumps adjacent to the inner aspect of the nuclear envelope (Fig. 2).

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