Relationship between stage of follicular development and RNA synthesis in the mouse oocyte

RNA synthesis, previously shown to take place during oocyte growth, has been demonstrated throughout the growth-quiescent period preceding ovulation of the mouse oocyte. In the final 7-day preovulatory period, the level of incorporation of (5,6-3H)uridine into ovulated oocytes decreased as the interval between exposure to precursor and ovulation decreased; significant incorporation was detectable within 2 days before ovulation. Analysis of the frequency and density of label in ovarian oocytes at successive stages of meiosis in relation to the interval between adminstration of labeled precursor and collection of oocytes revealed that RNA synthesis continues up to within 2 h before GVBD.

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Morphological Changes in the Rat Granulosa Cell Surface During Differentiation Induced by Estradiol and Gonadotropins

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079851 ◽

1977 ◽

Vol 35 ◽

pp. 484-485

Author(s):

P. Bagavandoss ◽

JoAnne S. Richards ◽

A. Rees Midgley

Keyword(s):

Cell Surface ◽

Granulosa Cell ◽

Morphological Changes ◽

Follicular Development ◽

Female Rats ◽

Functional Changes ◽

Group 4 ◽

Group 3 ◽

Group 5 ◽

Group 2

During follicular development in the mammalian ovary, several functional changes occur in the granulosa cells in response to steroid hormones and gonadotropins (1,2). In particular, marked changes in the content of membrane-associated receptors for the gonadotropins have been observed (1).We report here scanning electron microscope observations of morphological changes that occur on the granulosa cell surface in response to the administration of estradiol, human follicle stimulating hormone (hFSH), and human chorionic gonadotropin (hCG).Immature female rats that were hypophysectcmized on day 24 of age were treated in the following manner. Group 1: control groups were injected once a day with 0.1 ml phosphate buffered saline (PBS) for 3 days; group 2: estradiol (1.5 mg/0.2 ml propylene glycol) once a day for 3 days; group 3: estradiol for 3 days followed by 2 days of hFSH (1 μg/0.1 ml) twice daily, group 4: same as in group 3; group 5: same as in group 3 with a final injection of hCG (5 IU/0.1 ml) on the fifth day.

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Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066796 ◽

1971 ◽

Vol 29 ◽

pp. 548-549

Author(s):

Awtar Krishan ◽

Dora Hsu

Keyword(s):

Granular Material ◽

Rna Synthesis ◽

Culture Medium ◽

Actinomycin D ◽

Metabolic Inhibitors ◽

Protein And Rna Synthesis ◽

Apparent Reduction ◽

Plant Alkaloids ◽

In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

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Mono-follicular development is mediated via inhihin B pulses

Journal of the Society for Gynecologic Investigation ◽

10.1016/s1071-5576(97)86347-2 ◽

1998 ◽

Vol 5 (1) ◽

pp. 106A-106A

Author(s):

G LOCKWOOD ◽

S MUTTUKRISHNA ◽

N GROOME ◽

W LEDGER

Keyword(s):

Follicular Development

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Protein Synthesis DNA Synthesis and Repair RNA Synthesis Energy-Linked ATPases Synthetases

10.1016/s1874-6047(08)x6008-1 ◽

1974 ◽

Keyword(s):

Protein Synthesis ◽

Dna Synthesis ◽

Rna Synthesis

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THE NUCLEAR RNA-SYNTHESIS IN THE ANTERIOR PITUITARY OF RATS

Acta Endocrinologica ◽

10.1530/acta.0.049s160 ◽

1965 ◽

Vol 49 (3_Suppl) ◽

pp. S160 ◽

Cited By ~ 2

Author(s):

E. Stöcker ◽

G. Dhom

Keyword(s):

Anterior Pituitary ◽

Rna Synthesis ◽

Nuclear Rna Synthesis ◽

Nuclear Rna

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FSH AND LH LEVELS IN THE INTACT AND UNILATERALLY OVARIECTOMIZED CYCLING RAT

Acta Endocrinologica ◽

10.1530/acta.0.0690267 ◽

1972 ◽

Vol 69 (2) ◽

pp. 267-280 ◽

Cited By ~ 7

Author(s):

Richard D. Peppler

Keyword(s):

Plasma Concentrations ◽

Follicular Development ◽

Slight Modification ◽

Oestrous Cycle ◽

Successive Cycle ◽

Unilateral Ovariectomy ◽

Subsequent Cycle ◽

Intact Rats

ABSTRACT Intact 5-day cycling rats were killed between 8–10 a. m. on each day of the oestrous cycle; experimental rats were unilaterally ovariectomized (ULO) at 9 a. m. on day 1 (oestrus) and killed between 8 and 10 a. m. on days 2, 3, 4 or 1 of the subsequent cycle. Pituitary and plasma concentrations of FSH and LH were measured in both groups of rats. Pituitary FSH concentration was measured by the Steelman-Pohley method with slight modification; plasma FSH by the Igarashi-McCann assay and pituitary and plasma LH concentration by the OAAD method. In intact rats, pituitary FSH values remained constant for the first three days of the cycle, increased on day 4 and reverted to early cycle values by day 5. Plasma FSH increased between days 2 and 3 and days 5 and 1. Pituitary LH concentration remained the same for days 1 and 2; increased two-fold on days 3 and 4, and increased further by day 5. Plasma LH increased between days 2 and 3; other differences between successive cycle days were not apparent. Following ULO on day 1, pituitary FSH increased steadily, but not significantly, for the remaining cycle. Plasma FSH did not change from day 2 through day 1 of the subsequent cycle. Pituitary LH remained low on day 2, increased sharply by day 3 and decreased (50 %) by day 4. Plasma LH also increased between days 2 and 3. Other differences between successive days following unilateral ovariectomy on day 1 were not apparent. Correlation of gonadotrophin activity with follicular development suggests that the mechanism of compensatory ovulation in the rat may be one of an increase in time of exposure to a constant gonadotrophic level for the duration of the oestrous cycle rather than to increased levels of the gonadotrophin.

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FEMALE ENDOCRINE CONTROL MECHANISMS DURING THE NEONATAL PERIOD

Acta Endocrinologica ◽

10.1530/acta.0.0700396 ◽

1972 ◽

Vol 70 (2) ◽

pp. 396-408 ◽

Cited By ~ 1

Author(s):

K.-D. Schulz ◽

H. Haarmann ◽

A. Harland

Keyword(s):

Protein Synthesis ◽

Reproductive System ◽

Rna Synthesis ◽

Neonatal Period ◽

High Dose ◽

Female Reproductive System ◽

Endocrine Control ◽

Hypothalamic Region ◽

Rna And Protein Synthesis ◽

Physiological Dose

ABSTRACT The present investigation deals with the oestrogen-sensitivity of the female reproductive system during the neonatal period. Newborn female guinea pigs were used as test animals. At different times after a single subcutaneous injection of a physiological dose of 0.1 μg or an unphysiologically high dose of 10 μg 17β-oestradiol/100 g body weight, the RNA- and protein-synthesis was examined in the hypothalamic region, pituitary, cerebral cortex, liver, adrenal gland, ovary and uterus. With a physiological dose an increase in organ weight, protein content, RNA-and protein-synthesis was found only in the uterus. These alterations turned out to be dose-dependent. In addition to the findings in the uterus an inhibition of the aminoacid incorporation rate occurred in the liver following the injection of the high oestradiol dose. As early as 1 hour after the administration of 0.1 μg 17β-oestradiol an almost 100% increase in uterine protein synthesis was detectable. This result demonstrates a high oestrogen-sensitivity of this organ during the neonatal period. All the other organs of the female reproductive system such as the hypothalamus, pituitary and ovary did not show any oestrogen response. Therefore the functional immaturity of the uterus during post partem life is not the result of a deficient hormone sensitivity but is correlated with the absence of a sufficient hormonal stimulus at this time. The investigation on the effects of actinomycin resulted in different reactions in the uterus and liver. In contrast to the liver a paradoxical actinomycin effect was found in the uterus after treatment with actinomycin alone. This effect is characterized by a small inhibition of RNA-synthesis and a 50% increase in protein synthesis. The treatment of the newborn test animals with actinomycin and 17β-oestradiol together abolished the oestrogen-induced stimulation of the uterine RNA-and protein-synthesis. Consequently, the effect of oestrogens during the neonatal period is also connected with the formation of new proteins via an increased DNA-directed RNA-synthesis.

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