Age-dependent stimulation or inhibition of calcium release from bone cultures by interleukin-1β

The response to caffeine was studied in mouse muscles [diaphragm, soleus, and extensor digitorum longus (EDL)] with different ryanodine receptor isoform (RyR1, RyR3) composition and in single permeabilized muscle fibers dissected from diaphragm of wild-type (WT) and RyR3-deficient (RyR3−/−) mice at 1, 15, 30, and 60 postnatal days (PND). The caffeine response decreased during development, and, in adult mice, was greater in diaphragm, lower in EDL, and intermediate in soleus. This suggests a direct relation between response to caffeine and RyR3 expression. The lack of RyR3 reduced caffeine response in young, but not in adult mice, and did not abolish the age-dependent variation and the intermuscle differences. In diaphragm single fibers, the response to caffeine increased during development and was reduced in fibers lacking RyR3 both at 15 and 60 PND. A population of fibers highly responsive to caffeine was present in adult WT and disappeared in RyR3−/−. The results confirm the contribution of RyR3 to calcium release for contractile response and clarify the contribution of RyR3 to developmental changes and intermuscle differences.

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Retinoic acid induced proteoglycan release and cartilage resorption in rat bone cultures are age dependent and inhibited by EDTA

Wilhelm Roux s Archives of Developmental Biology ◽

10.1007/bf00848167 ◽

1979 ◽

Vol 187 (1) ◽

pp. 59-71 ◽

Cited By ~ 16

Author(s):

Andreas Kistler ◽

Brigitta Galli

Keyword(s):

Retinoic Acid ◽

Cartilage Resorption ◽

Age Dependent ◽

Proteoglycan Release ◽

And Cartilage ◽

Rat Bone ◽

Bone Cultures

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Hsp90 mediates insulin-like growth factor 1 and interleukin-1β signaling in an age-dependent manner in equine articular chondrocytes

Arthritis & Rheumatism ◽

10.1002/art.22664 ◽

2007 ◽

Vol 56 (7) ◽

pp. 2335-2343 ◽

Cited By ~ 20

Author(s):

Amber K. Boehm ◽

Mayank Seth ◽

Karen G. Mayr ◽

Lisa A. Fortier

Keyword(s):

Growth Factor ◽

Articular Chondrocytes ◽

Interleukin 1Β ◽

Dependent Manner ◽

Insulin Like Growth Factor ◽

Age Dependent

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Membrane Changes in Polymorphonuclear Leukocytes During Ionophore (A23187) - Induced Lysosomal Release

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010007984x ◽

1977 ◽

Vol 35 ◽

pp. 482-483

Author(s):

P.L. Moore ◽

P.L. Sannes ◽

H.L. Bank ◽

S.S. Spicer

Keyword(s):

Structural Changes ◽

Calcium Release ◽

Clear Understanding ◽

Ionophore A23187 ◽

Enzyme Release ◽

Extracellular Medium ◽

Pmn Leukocytes ◽

Intracellular Ion Concentrations ◽

Membrane Changes ◽

Pmn Leukocyte

It is thought that calcium and/or magnesium may play important roles in polymorphonuclear (PMN) leukocyte functions such as chemotaxis, adhesion and phagocytosis. Yet, a clear understanding of the biological roles of these ions has awaited the development of techniques which permit a selective alteration of intracellular ion concentrations. Recently, treatment of cells with the ionophore A23187 has been used to alter intracellular divalent cation concentrations. This ionophore is a lipid soluble antibiotic produced by Streptomyces chartreusensis that complexes with both calcium and magnesium (3) and is believed to carry these ions across biological membranes (4). Biochemical investigations of human PMN leukocytes demonstrate that cells treated with A23187 and extracellular calcium release their lysosomal enzymes into the extracellular medium without rupturing and releasing their soluble cytoplasmic enzymes (5,6). The aim of the present study and and a companion report (7) was to investigate the structural changes that occur in leukocytes during ionophore-induced lysosomal enzyme release.

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Effects of Hyperoxia on Lung Utrastructure

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100067121 ◽

1970 ◽

Vol 28 ◽

pp. 26-27

Author(s):

Gladys Harrison

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Light Microscopy ◽

Oxygen Effects ◽

Age Dependent ◽

The Central Nervous System ◽

The Subject ◽

Space Age ◽

Alveolar Septa ◽

Extensive Damage

With the advent of the space age and the need to determine the requirements for a space cabin atmosphere, oxygen effects came into increased importance, even though these effects have been the subject of continuous research for many years. In fact, Priestly initiated oxygen research when in 1775 he published his results of isolating oxygen and described the effects of breathing it on himself and two mice, the only creatures to have had the “privilege” of breathing this “pure air”.Early studies had demonstrated the central nervous system effects at pressures above one atmosphere. Light microscopy revealed extensive damage to the lungs at one atmosphere. These changes which included perivascular and peribronchial edema, focal hemorrhage, rupture of the alveolar septa, and widespread edema, resulted in death of the animal in less than one week. The severity of the symptoms differed between species and was age dependent, with young animals being more resistant.

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