Effect of somatic cell type on bovine embryonic development in co-culture

1991 ◽  
Vol 35 (1) ◽  
pp. 269 ◽  
Author(s):  
J.M. Scodras ◽  
J.W. Pollard ◽  
K.J. Betteridge
Keyword(s):  
Embryonic Development ◽  
Somatic Cell ◽  
Cell Type ◽  
Somatic Cell Type

scholarly journals Nuclear reprogramming in cell–free extracts

2003 ◽  
Vol 358 (1436) ◽  
pp. 1389-1395 ◽  
Author(s):  
Philippe Collas
Keyword(s):  
Somatic Cell ◽  
Adult Stem Cells ◽  
Regulatory Function ◽  
Nuclear Reprogramming ◽  
Cell Type ◽  
Differentiation Potential ◽  
Chromatin Remodelling Complex ◽  
Therapeutic Applications ◽  
Somatic Cell Type

Methods for directly turning a somatic cell type into another type (a process referred to as transdifferentiation) would be beneficial for producing replacement cells for therapeutic applications. Adult stem cells have been shown to display a broader differentiation potential than anticipated and may contribute to tissues other than those in which they reside. In addition, novel transdifferentiation strategies are being developed. I report recent results on the functional reprogramming of a somatic cell using a nuclear and cytoplasmic extract derived from another somatic cell type. The reprogramming of 293T fibroblasts in an extract from T cells is evidenced by nuclear uptake and the assembly of transcription factors, induction of activity of a chromatin remodelling complex, changes in chromatin composition and activation of lymphoid cell–specific genes. The reprogrammed cells express T–cell–specific surface molecules and a complex regulatory function. Reprogramming cells in cell–free extracts may create possibilities for producing replacement cells for therapeutic applications. The system may also constitute a powerful tool to examine the mechanisms of nuclear reprogramming, at least as they occur in vitro .

Regeneration of a Complete Hydra from a Single, Differentiated Somatic Cell Type

1973 ◽  
pp. 255-267 ◽  
Author(s):  
ALLISON L. BURNETT ◽  
RALPH LOWELL ◽  
MARSHALL N. CYRLIN
Keyword(s):  
Somatic Cell ◽  
Cell Type ◽  
Somatic Cell Type

scholarly journals Somatic cell type specific gene transfer reveals a tumor-promoting function for p21Waf1/Cip1

2007 ◽  
Vol 26 (22) ◽  
pp. 4683-4693 ◽  
Author(s):  
Yuhui Liu ◽  
Nancy Yeh ◽  
Xin-Hua Zhu ◽  
Margaret Leversha ◽  
Carlos Cordon-Cardo ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Somatic Cell ◽  
Specific Gene ◽  
Cell Type ◽  
Cell Type Specific ◽  
Somatic Cell Type

scholarly journals GC-1 spg cells are most similar to Leydig cells, a testis somatic cell-type, and not germ cells

2021 ◽  
Author(s):  
Andrew R Norman ◽  
Lauren Byrnes ◽  
Jeremy R Reiter
Keyword(s):  
Germ Cell ◽  
Somatic Cell ◽  
Cell Line ◽  
Germ Cells ◽  
Leydig Cells ◽  
Adult Mouse ◽  
Somatic Cells ◽  
Cell Type ◽  
Immortalized Cell ◽  
Somatic Cell Type

GC-1 spg is an immortalized cell line derived from an adult mouse testis and reported to be most similar to spermatocytes, a male germ cell-type. However, immunofluorescence indicates that GC-1 spg cells express WT1, a marker of testis somatic cells, and do not express markers of germ cells. Transcriptomic profiling indicate GC-1 cells are most similar to Leydig cells. Therefore, we conclude that GC-1 spg cells are most similar to testis somatic cells.

The Efficiency of Cell Fusion-Based Reprogramming Is Affected by the Somatic Cell Type and the In Vitro Age of Somatic Cells

2011 ◽  
Vol 13 (4) ◽  
pp. 331-344 ◽  
Author(s):  
Pollyanna Agnes Tat ◽  
Huseyin Sumer ◽  
Daniele Pralong ◽  
Paul John Verma
Keyword(s):  
Somatic Cell ◽  
Cell Fusion ◽  
Somatic Cells ◽  
Cell Type ◽  
Somatic Cell Type

scholarly journals Effect of D-Glucuronic Acid and N-acetyl-D-Glucosamine Treatment during In Vitro Maturation on Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1034
Author(s):  
Joohyeong Lee ◽  
Eunhye Kim ◽  
Seon-Ung Hwang ◽  
Lian Cai ◽  
Mirae Kim ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
In Vitro Maturation ◽  
Glucuronic Acid ◽  
Cumulus Cell ◽  
Cortical Granule ◽  
Oocyte Diameter

This study aimed to examine the effects of treatment with glucuronic acid (GA) and N-acetyl-D-glucosamine (AG), which are components of hyaluronic acid (HA), during porcine oocyte in vitro maturation (IVM). We measured the diameter of the oocyte, the thickness of the perivitelline space (PVS), the reactive oxygen species (ROS) level, and the expression of cumulus cell expansion and ROS-related genes and examined the cortical granule (CG) reaction of oocytes. The addition of 0.05 mM GA and 0.05 mM AG during the first 22 h of oocyte IVM significantly increased oocyte diameter and PVS size compared with the control (non-treatment). The addition of GA and AG reduced the intra-oocyte ROS content and improved the CG of the oocyte. GA and AG treatment increased the expression of CD44 and CX43 in cumulus cells and PRDX1 and TXN2 in oocytes. In both the chemically defined and the complex medium (Medium-199 + porcine follicular fluid), oocytes derived from the GA and AG treatments presented significantly higher blastocyst rates than the control after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT). In conclusion, the addition of GA and AG during IVM in pig oocytes has beneficial effects on oocyte IVM and early embryonic development after PA and SCNT.

Glucose in a maturation medium with reduced NaCl improves oocyte maturation and embryonic development after somatic cell nuclear transfer and in vitro fertilization in pigs

Zygote ◽  
2021 ◽  
pp. 1-8
Author(s):  
Yongjin Lee ◽  
Hanna Lee ◽  
Joohyeong Lee ◽  
Seung Tae Lee ◽  
Geun-Shik Lee ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Somatic Cell ◽  
Oocyte Maturation ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Blastocyst Formation ◽  
Nuclear Maturation ◽  
Maturation Medium ◽  
Glucose Supplementation

Summary This study was conducted to examine whether glucose in maturation medium containing reduced NaCl could improve oocyte maturation and embryonic development in pigs. The base medium was bovine serum albumin-free porcine zygote medium (PZM)-3 containing 10% (v/v) pig follicular fluid (FPZM) or 0.1% (w/v) polyvinyl alcohol (PPZM). Using each medium, the effects of NaCl concentrations (108 and 61.6 mM) and 5.56 mM glucose supplementation (designated as PZM108N, PZM108G, PZM61N, and PZM61G, respectively) were examined using a 2 × 2 factorial arrangement. When oocytes were matured in FPZM, glucose supplementation improved nuclear maturation compared with no supplementation, regardless of the NaCl concentrations. FPZM61G showed a higher blastocyst formation compared with FPZM108N and FPZM108G after parthenogenesis (PA). Blastocyst formations of somatic cell nuclear transfer (SCNT) embryos derived from FPZM61N and FPZM61G were higher compared with those of oocytes from FPZM108N. When oocytes were matured in PPZM, glucose added to PPZM108 and PPZM61 increased nuclear maturation compared with no supplementation. However, glucose added to PPZM108 did not alter embryonic development after PA. Additionally, oocytes matured in PPZM61G showed a higher blastocyst formation compared with those from PPZM61N. In SCNT, blastocyst formation was not influenced by glucose supplementation of PPZM108, but was increased by maturation in glucose-supplemented PPZM61. In embryonic development of in vitro fertilization (IVF), oocytes matured in medium with reduced NaCl and glucose showed significantly higher blastocyst formation compared with those matured in PPZM108G. Our results demonstrated that glucose in maturation medium containing 61.6 mM NaCl increased oocyte maturation and embryonic development after PA, SCNT, and IVF.

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