The effect of free radicals and anti-oxidant during in vitro maturation and fertilization of porcine oocytes

AbstractFree radicals are important mediators in a number of neurodegenerative diseases and molecules capable of scavenging reactive oxygen species (ROS) may be a feasible strategy for protecting neuronal cells. In this sense, polyphenols have been studied for their antioxidant effects, KM-34 (5-(3, 4-dydroxyl-benzylidene)-2, 2-dimethyl-1, 3-dioxane-4, 6-Dione) is a novel synthetic catechol with potential neuroprotective and antioxidant properties. We have assessed the antioxidant (as scavenging and iron-chelating compound) and neuroprotectant in vitro (in PC12 cell injury induced by H2O2, glutamate or FeSO4/AA) of KM-34. KM-34 was found to be a potent antioxidant, as shown by (i) inhibition of iron induced-brain lipid peroxidation, (ii) inhibition of 2-deoxyribose degradation, (iii) inhibition of superoxide radicals generation (IC50=11.04 μM) and (iv) inhibition of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical reduction (IC50=16.26 μM). The overall anti-oxidant action of KM-34 appears to be a combination of a direct reaction with free radicals and chelating the metal ions responsible for the production of ROS. Our work suggests that the antioxidant properties of KM-34 may provide future therapeutic approaches for neurodegenerative disorders.

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Protective effect of Aurelia aurita against free radicals and streptozotocin-induced diabetes

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v13i3.36907 ◽

2018 ◽

Vol 13 (3) ◽

pp. 287 ◽

Cited By ~ 1

Author(s):

Mallikarjuna Rao Talluri ◽

Alekhya Ketha ◽

Ganga Rao Battu ◽

Rajananda Swamy Tadi ◽

Vinay Bharadwaj Tatipamula

Keyword(s):

Free Radicals ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Aurelia Aurita ◽

Biologically Active ◽

Chemical Profiling ◽

Streptozotocin Induced Diabetes ◽

Anti Oxidant ◽

Profiling Analysis

The present work was carried out to identify the anti-oxidant and anti-diabetic activities of Aurelia aurita. The chemical profiling analysis showed that it possess different biologically active secondary metabolites like phenols, alakoids, steroids etc. The methanolic extract showed different free radical scavenging activity as ascorbic acid with IC50 values 202, 205, 153 µg on DPPH, hydroxyl and superoxide free radicals. The extract significantly reduced the hyperglycemic conditions with percentage of reduction 18.7 ± 1.3 to 53.5 ±1.5 of streptozotocin-induced animals and the positive result of in-vitro aldose reductase enzyme inhibition with IC50 value 163 µg suggests that A. aurita have potential to cure the diabetic complications.

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Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenetic protein 15 and follicle stimulating hormone

Molecular Reproduction and Development ◽

10.1002/mrd.22470 ◽

2015 ◽

Vol 82 (4) ◽

pp. 281-294 ◽

Cited By ~ 29

Author(s):

Melanie L. Sutton-McDowall ◽

Malcolm Purdey ◽

Hannah M. Brown ◽

Andrew D. Abell ◽

David G. Mottershead ◽

...

Keyword(s):

Bone Morphogenetic Protein ◽

In Vitro Maturation ◽

Follicle Stimulating Hormone ◽

Morphogenetic Protein ◽

Anti Oxidant ◽

Bone Morphogenetic Protein 15 ◽

Stimulating Hormone

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Mechanisms of oxidative stress in porcine oocytes and the role of anti-oxidants

Reproduction Fertility and Development ◽

10.1071/rd08037 ◽

2008 ◽

Vol 20 (6) ◽

pp. 694 ◽

Cited By ~ 28

Author(s):

B. D. Whitaker ◽

J. W. Knight

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Free Radicals ◽

Dna Fragmentation ◽

Reduced Glutathione ◽

Sod Activity ◽

Anti Oxidant ◽

Gpx Activity

The mechanisms of oxidative stress in in vitro maturing porcine oocytes and the effects of anti-oxidant supplementation of the medium in ameliorating these effects were investigated in the present study. In addition to intracellular reduced glutathione (GSH) concentrations and DNA fragmentation, the present study focused on superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase activity. The anti-oxidants used were N-acetylcysteine (NAC) and its derivative NAC-amide (NACA). The results indicate that when SOD is inhibited, supplementation of the maturarion medium with 1.5 mm NAC or NACA compensates for the decrease in SOD activity by reducing the degree of DNA fragmentation (P < 0.05). When GPx is inhibited, supplementation of the maturarion medium with 1.5 mm NAC alleviates the effects of no GPx activity, as indicated by a decrease in the degree of DNA fragmentation (P < 0.05). When the maturarion medium was supplemented with 1.5 mm NACA, intracellular GSH concentrations decreased (P < 0.05) and SOD and catalase activities increased (P < 0.05) along with the degree of DNA fragmentation. These results indicate that the mechanisms of alleviating oxidative stress in porcine oocytes are very complex and supplementing maturing oocytes with anti-oxidants may enhance enzyme activities and eliminate free radicals.

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GC-MS, phytochemical analysis and in vitro antioxidant activities of leaves of Canavalia cathartica Thouars

The Journal of Phytopharmacology ◽

10.31254/phyto.2018.7306 ◽

2018 ◽

Vol 7 (3) ◽

pp. 263-269

Author(s):

Saraswathi K ◽

◽

Rajesh V ◽

Saranya R ◽

Arumugam P ◽

...

Keyword(s):

Free Radicals ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Phytochemical Analysis ◽

Nitric Oxide Radical ◽

Effective Antioxidant ◽

Anti Oxidant ◽

Reducing Power Assay

Plants have been used for thousands of years to flavour and conserve food, to treat health disorders and to prevent diseases including epidemics. The knowledge of the anti-oxidant properties has been a promising method of assessing free radicals damage. The aim of the present study was to evaluate the antioxidant activities of leaves of Canavalia cathartica and to identify the bioactive compounds by performing GC-MS analysis resulting in the presence of volatile and semi volatile compounds. The IC50 of DPPH˙ radical, ABTS˙+ radical cation, Nitric oxide radical scavenging assays were 84.03, 51.18 and 351.78µg/mL concentration respectively. Also, the IC50 of Phosphomolybdenum reduction and ferric reducing power assay were 81.53 and 87.64µg/mL concentration respectively. The results of this study portray the effective antioxidant activity of Canavalia cathartica and further studies are required to isolate the active compounds from various parts of this species and their mode of action. From the study it can be concluded that the plant might be promising as a curative for many diseases associated with free radicals

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In vitro maturation of primate oocytes retrieved at the time of cesarean section

Journal of the Society for Gynecologic Investigation ◽

10.1016/s1071-5576(97)86351-4 ◽

1998 ◽

Vol 5 (1) ◽

pp. 106A-106A

Author(s):

R BRZYSKI ◽

M LELAND ◽

C EDDY

Keyword(s):

Cesarean Section ◽

In Vitro Maturation

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Effect of isoflavone compounds on the in vitro maturation of sheep oocytes

Planta Medica ◽

10.1055/s-0035-1565604 ◽

2015 ◽

Vol 81 (16) ◽

Author(s):

AA Amir ◽

GB Martin ◽

JM Kelly ◽

DO Kleemann ◽

Z Durmic ◽

...

Keyword(s):

In Vitro Maturation

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Protective Effect of Vitamin E on Immune Triggered, Granulocyte Mediated Endothelial Injury

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661027 ◽

1984 ◽

Vol 51 (01) ◽

pp. 089-092 ◽

Cited By ~ 24

Author(s):

M A Boogaerts ◽

J Van de Broeck ◽

H Deckmyn ◽

C Roelant ◽

J Vermylen ◽

...

Keyword(s):

Vitamin E ◽

Protective Effect ◽

Umbilical Vein ◽

Endothelial Damage ◽

Endothelial Injury ◽

Mediated Effects ◽

Anti Oxidant ◽

Endothelial Cell Cultures ◽

Vitro Model

SummaryThe effect of alfa-tocopherol on the cell-cell interactions at the vessel wall were studied, using an in vitro model of human umbilical vein endothelial cell cultures (HUEC). Immune triggered granulocytes (PMN) will adhere to and damage HUEC and platelets enhance this PMN mediated endothelial injury. When HUEC are cultured in the presence of vitamin E, 51Cr-leakage induced by complement stimulated PMN is significantly decreased and the enhanced cytotoxicity by platelets is completely abolished (p <0.001).The inhibition of PMN induced endothelial injury is directly correlated to a diminished adherence of PMN to vitamin E- cultured HUEC (p <0.001), which may be mediated by an increase of both basal and stimulated endogenous prostacyclin (PGI2) from alfa-tocopherol-treated HUEC (p <0.025). The vitamin E-effect is abolished by incubation of HUEC with the irreversible cyclo-oxygenase inhibitor, acetylsalicylic acid, but the addition of exogenous PGI2 could not reproduce the vitamin E-mediated effects.We conclude that vitamin E exerts a protective effect on immune triggered endothelial damage, partly by increasing the endogenous anti-oxidant potential, partly by modulating intrinsic endothelial prostaglandin production. The failure to reproduce vitamin E-protection by exogenously added PGI2 may suggest additional, not yet elucidated vitamin E-effects on endothelial metabolism.

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